Rapid stimulation of diacylglycerol production in Xenopus oocytes by microinjection of H-ras p21 - PubMed (original) (raw)
Comparative Study
. 1987 Oct 23;238(4826):533-6.
doi: 10.1126/science.2821623.
Affiliations
- PMID: 2821623
- DOI: 10.1126/science.2821623
Comparative Study
Rapid stimulation of diacylglycerol production in Xenopus oocytes by microinjection of H-ras p21
J C Lacal et al. Science. 1987.
Abstract
The p21 products of ras proto-oncogenes are thought to be important components in pathways regulating normal cell proliferation and differentiation. These proteins acquire transforming properties as a result of activating lesions that convert ras genes to oncogenes in a wide array of malignancies. In Xenopus laevis oocytes, microinjection of transforming ras p21 is a potent inducer of maturation, whereas microinjection of a monoclonal antibody to ras p21 inhibits normal maturation induced by hormones. The phosphoinositide pathway is a ubiquitous system that appears to play a key role in diverse cellular functions. By use of the Xenopus oocyte system, it was possible to quantitate the effects of ras p21 microinjection on individual components of the phosphoinositide pathway. Within 20 minutes of microinjection, levels of phosphatidylinositol 4,5-bisphosphate, inositol 1-phosphate, and inositol bisphosphate increased 1.5- to 2-fold. The most striking effects were on diacylglycerol, which increased 5-fold under the same conditions. In contrast, the normal ras p21 protein induced no detectable alteration in any of the metabolites analyzed. The earliest effects of the transforming p21 on phosphoinositol turnover were observable within 2 minutes, implying a very rapid effect of ras p21 on the enzymes involved in phospholipid metabolism.
Similar articles
- Requirement of phospholipase C-catalyzed hydrolysis of phosphatidylcholine for maturation of Xenopus laevis oocytes in response to insulin and ras p21.
García de Herreros A, Dominguez I, Diaz-Meco MT, Graziani G, Cornett ME, Guddal PH, Johansen T, Moscat J. García de Herreros A, et al. J Biol Chem. 1991 Apr 15;266(11):6825-9. J Biol Chem. 1991. PMID: 2016297 - Role of phosphatidylinositide metabolism in ras-induced Xenopus oocyte maturation.
Pan BT, Cooper GM. Pan BT, et al. Mol Cell Biol. 1990 Mar;10(3):923-9. doi: 10.1128/mcb.10.3.923-929.1990. Mol Cell Biol. 1990. PMID: 2154685 Free PMC article. - Pathways for activation of the ras-oncogene-encoded p21 protein.
Pincus MR, Chung D, Dykes DC, Brandt-Rauf P, Weinstein IB, Yamaizumi Z, Nishimura S. Pincus MR, et al. Ann Clin Lab Sci. 1992 Sep-Oct;22(5):323-42. Ann Clin Lab Sci. 1992. PMID: 1524403 Review. - ras proteins: biological effects and biochemical targets (review).
Bar-Sagi D. Bar-Sagi D. Anticancer Res. 1989 Sep-Oct;9(5):1427-37. Anticancer Res. 1989. PMID: 2686537 Review.
Cited by
- Ras oncogenes: split personalities.
Karnoub AE, Weinberg RA. Karnoub AE, et al. Nat Rev Mol Cell Biol. 2008 Jul;9(7):517-31. doi: 10.1038/nrm2438. Nat Rev Mol Cell Biol. 2008. PMID: 18568040 Free PMC article. Review. - Diacylglycerol Levels Unchanged during Auxin-Stimulated Growth of Excised Hypocotyl Segments of Soybean.
Morré DJ, Pfaffmann H, Drobes B, Wilkinson FE, Hartmann E. Morré DJ, et al. Plant Physiol. 1989 May;90(1):275-9. doi: 10.1104/pp.90.1.275. Plant Physiol. 1989. PMID: 16666748 Free PMC article. - Cyclic AMP decreases chemotaxis, invasiveness and lung colonization of H-ras transformed mouse fibroblasts.
Iwamoto Y, Reich R, Nemeth G, Yamada Y, Martin GR. Iwamoto Y, et al. Clin Exp Metastasis. 1993 Nov;11(6):492-501. doi: 10.1007/BF00054940. Clin Exp Metastasis. 1993. PMID: 7693388 - NIH-3T3 cells transformed by the EJ-ras oncogene exhibit reduced platelet-derived growth factor-mediated Ca2+ mobilization.
Benjamin CW, Connor JA, Tarpley WG, Gorman RR. Benjamin CW, et al. Proc Natl Acad Sci U S A. 1988 Jun;85(12):4345-9. doi: 10.1073/pnas.85.12.4345. Proc Natl Acad Sci U S A. 1988. PMID: 3288991 Free PMC article. - Desensitization of the Ca2+-mobilizing system to serum growth factors by Ha-ras and v-mos.
Maly K, Doppler W, Oberhuber H, Meusburger H, Hofmann J, Jaggi R, Grunicke HH. Maly K, et al. Mol Cell Biol. 1988 Oct;8(10):4212-6. doi: 10.1128/mcb.8.10.4212-4216.1988. Mol Cell Biol. 1988. PMID: 3141785 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
Miscellaneous