The v-mos and H-ras oncogene expression represses glucocorticoid hormone-dependent transcription from the mouse mammary tumor virus LTR - PubMed (original) (raw)
The v-mos and H-ras oncogene expression represses glucocorticoid hormone-dependent transcription from the mouse mammary tumor virus LTR
R Jaggi et al. EMBO J. 1986 Oct.
Abstract
We have subjected the viral mos oncogene (v-mos), the activated human H-ras oncogene [H-ras (A)] and the normal human H-ras protooncogene [H-ras (N)] to the transcriptional regulation of glucocorticoid hormones by in vitro recombination with the promoter region of the mouse mammary tumor virus long terminal repeat (MMTV LTR) and transfection into NIH 3T3 cells. Cell clones were selected which exhibit a transformed phenotype strictly dependent on the presence of hormone in the growth medium. The expression of the chimeric genes as a function of time after hormone stimulation was studied at the level of transcriptional rate, mRNA and protein accumulation. Oncogene expression was stimulated rapidly to high levels, after hormone addition, but declined in the continuous presence of hormone. Measurements of the transcriptional rates in nuclei from LTR v-mos and LTR H-ras (A) transfected cells showed a repression of LTR v-mos and LTR H-ras (A) transcription after the initial stimulation by hormone. LTR H-ras (N) transcription was not affected. An independently transfected LTR H-2Ld construct in LTR v-mos or LTR H-ras (A) containing cells is also transcriptionally repressed. These experiments demonstrated a transcriptional repression effect of the oncogene products on the glucocorticoid hormone-dependent MMTV LTR transcription.
Similar articles
- Tumor-promoting phorbol ester and ras oncogene expression inhibit the glucocorticoid-dependent transcription from the mouse mammary tumor virus long terminal repeat.
Vacca A, Screpanti I, Maroder M, Petrangeli E, Frati L, Gulino A. Vacca A, et al. Mol Endocrinol. 1989 Oct;3(10):1659-65. doi: 10.1210/mend-3-10-1659. Mol Endocrinol. 1989. PMID: 2558300 - Transcription initiation of transfected mouse mammary tumor virus LTR DNA is regulated by glucocorticoid hormones.
Groner B, Hynes NE, Rahmsdorf U, Ponta H. Groner B, et al. Nucleic Acids Res. 1983 Jul 25;11(14):4713-25. doi: 10.1093/nar/11.14.4713. Nucleic Acids Res. 1983. PMID: 6308557 Free PMC article. - Oncogene mediated repression of glucocorticoid hormone response elements and glucocorticoid receptor levels.
Jaggi R, Höck W, Ziemiecki A, Klemenz R, Friis R, Groner B. Jaggi R, et al. Cancer Res. 1989 Apr 15;49(8 Suppl):2266s-2274s. Cancer Res. 1989. PMID: 2564809 - Cooperation between structural elements in hormono-regulated transcription from the mouse mammary tumor virus promoter.
Gouilleux F, Sola B, Couette B, Richard-Foy H. Gouilleux F, et al. Nucleic Acids Res. 1991 Apr 11;19(7):1563-9. doi: 10.1093/nar/19.7.1563. Nucleic Acids Res. 1991. PMID: 1851294 Free PMC article. - Oncogenes modulate cellular gene expression and repress glucocorticoid regulated gene transcription.
Jaggi R, Friis R, Groner B. Jaggi R, et al. J Steroid Biochem. 1988 May;29(5):457-63. doi: 10.1016/0022-4731(88)90179-3. J Steroid Biochem. 1988. PMID: 3379954
Cited by
- Attenuation of glucocorticoid receptor levels by the H-ras oncogene.
Martins VR, Brentani MM, Housley PR. Martins VR, et al. Endocrine. 1995 Apr;3(4):305-12. doi: 10.1007/BF03021410. Endocrine. 1995. PMID: 21153179 - Construction and characterization of a hybrid mouse mammary tumor virus/murine leukemia virus-based retroviral vector.
Saller RM, Oztürk F, Salmons B, Günzburg WH. Saller RM, et al. J Virol. 1998 Feb;72(2):1699-703. doi: 10.1128/JVI.72.2.1699-1703.1998. J Virol. 1998. PMID: 9445081 Free PMC article. - Mitogen-activated and cyclin-dependent protein kinases selectively and differentially modulate transcriptional enhancement by the glucocorticoid receptor.
Krstic MD, Rogatsky I, Yamamoto KR, Garabedian MJ. Krstic MD, et al. Mol Cell Biol. 1997 Jul;17(7):3947-54. doi: 10.1128/MCB.17.7.3947. Mol Cell Biol. 1997. PMID: 9199329 Free PMC article. - Steroid hormone receptors activate transcription in glial cells of intact retina but not in primary cultures of retinal glial cells.
Li YC, Hayes S, Young AP. Li YC, et al. J Mol Neurosci. 1997 Apr;8(2):145-58. doi: 10.1007/BF02736779. J Mol Neurosci. 1997. PMID: 9188043 - Alpha B-crystallin expression in mouse NIH 3T3 fibroblasts: glucocorticoid responsiveness and involvement in thermal protection.
Aoyama A, Fröhli E, Schäfer R, Klemenz R. Aoyama A, et al. Mol Cell Biol. 1993 Mar;13(3):1824-35. doi: 10.1128/mcb.13.3.1824-1835.1993. Mol Cell Biol. 1993. PMID: 8441415 Free PMC article.
References
- Cell. 1983 Sep;34(2):557-67 - PubMed
- Mol Cell Biol. 1985 Nov;5(11):2984-92 - PubMed
- Cell. 1983 Dec;35(2 Pt 1):381-92 - PubMed
- Cell. 1984 Feb;36(2):403-11 - PubMed
- Cell. 1984 Mar;36(3):577-9 - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials
Miscellaneous