Partial purification and characterization of a binding protein for insulin-like activity (ILAs) in human amniotic fluid: a possible inhibitor of insulin-like activity - PubMed (original) (raw)
Partial purification and characterization of a binding protein for insulin-like activity (ILAs) in human amniotic fluid: a possible inhibitor of insulin-like activity
S L Drop et al. Acta Endocrinol (Copenh). 1979 Mar.
Abstract
An insulin radioreceptor assay (INS-RRA) and an insulin-like activity radioreceptor assay (ILAs-RRA) have been utilized to partially purify and characterize a protein from human amniotic fluid with ILAs-RRA reactivity. An acid-ethanol soluble protein with an apparent molecular weight of 34,500 daltons by calibrated Sephadex chromatography and an isoelectric point (pI) of 4.7 accounts for all of the ILA'S-RRA reactivity present in human amniotic fluid. Since this protein has been found to be a binding protein for ILAs, but not insulin, it has been termed amniotic fluid binding protein or AFBP. AFBP is reactive in a non-parallel manner in the ILAs-RRA and totally inactive in the INS-RRA. The activity of AFBP in the ILAs-RRA is thus to the competition of AFBP with the placental membrane receptor for the [125I]ILAs tracer employed in the ILA'S-RRA. AFBP inhibits the activity of employed insulin, in the INS-RRA, presumably by binding ILAs, while being inactive itself. In two biological assays studied to date, the rat epididymal fat pad assay and the rabbit chondrocyte sulphation assay, AFBP also inhibits the activity of added ILAs. These observations raise the possibility that binding protein(s) for insulin-like peptides may function as inhibitors of their bioactivity in different physiologic and pathologic states. The relation of AFBP to binding protein(s) in human plasma remains to be clarified.
Similar articles
- Partial purification, characterization, and assay of a slightly acidic insulin-like peptide (ILAs) from human plasma.
Posner BI, Guyda HJ, Corvol MT, Rappaport R, Harley C, Goldstein S. Posner BI, et al. J Clin Endocrinol Metab. 1978 Dec;47(6):1240-50. doi: 10.1210/jcem-47-6-1240. J Clin Endocrinol Metab. 1978. PMID: 400741 - Isolation of a somatomedin-binding protein from preterm amniotic fluid. Development of a radioimmunoassay.
Drop SL, Kortleve DJ, Guyda HJ. Drop SL, et al. J Clin Endocrinol Metab. 1984 Nov;59(5):899-907. doi: 10.1210/jcem-59-5-899. J Clin Endocrinol Metab. 1984. PMID: 6207199 - [Purification and characterization of insulin-like growth factor binding proteins (IGF-BP) in amniotic fluid--existence of a 160K IGF-BP].
Iwashita M, Watanabe M, Adachi T, Takeda Y, Sakamoto S. Iwashita M, et al. Nihon Naibunpi Gakkai Zasshi. 1990 Jun 20;66(6):636-48. doi: 10.1507/endocrine1927.66.6_636. Nihon Naibunpi Gakkai Zasshi. 1990. PMID: 1696916 Japanese. - Immunoassay of a somatomedin-binding protein from human amniotic fluid: levels in fetal, neonatal, and adult sera.
Drop SL, Kortleve DJ, Guyda HJ, Posner BI. Drop SL, et al. J Clin Endocrinol Metab. 1984 Nov;59(5):908-15. doi: 10.1210/jcem-59-5-908. J Clin Endocrinol Metab. 1984. PMID: 6207200 - Characterization of a protein in mid-term human amniotic fluid which reacts in the somatomedin-C radioreceptor assay.
Chochinov RH, Mariz IK, Hajek AS, Daughaday WH. Chochinov RH, et al. J Clin Endocrinol Metab. 1977 May;44(5):902-8. doi: 10.1210/jcem-44-5-902. J Clin Endocrinol Metab. 1977. PMID: 870516
Cited by
- Insulin-like growth factor binding protein-3 protease activity in the urine of children with chronic renal failure.
Lee DY, Cohen P, Krensky AM, Rosenfeld RG, Yorgin PD. Lee DY, et al. Pediatr Nephrol. 1993 Aug;7(4):416-23. doi: 10.1007/BF00857555. Pediatr Nephrol. 1993. PMID: 7691141 - Inhibition of biological activity of multiplication-stimulating activity by binding to its carrier protein.
Knauer DJ, Smith GL. Knauer DJ, et al. Proc Natl Acad Sci U S A. 1980 Dec;77(12):7252-6. doi: 10.1073/pnas.77.12.7252. Proc Natl Acad Sci U S A. 1980. PMID: 7012835 Free PMC article. - An insulin-like growth factor (IGF) binding protein enhances the biologic response to IGF-I.
Elgin RG, Busby WH Jr, Clemmons DR. Elgin RG, et al. Proc Natl Acad Sci U S A. 1987 May;84(10):3254-8. doi: 10.1073/pnas.84.10.3254. Proc Natl Acad Sci U S A. 1987. PMID: 2953025 Free PMC article. - Somatomedins: chemical and functional characteristics of the different molecular forms.
Barreca A, Minuto F. Barreca A, et al. J Endocrinol Invest. 1989 Apr;12(4):279-93. doi: 10.1007/BF03349988. J Endocrinol Invest. 1989. PMID: 2545770 Review. No abstract available. - Somatomedin-binding proteins: what role do they play in the growth process?
Cianfarani S, Holly JM. Cianfarani S, et al. Eur J Pediatr. 1989 Nov;149(2):76-9. doi: 10.1007/BF01995850. Eur J Pediatr. 1989. PMID: 2480240 Review.
MeSH terms
Substances
LinkOut - more resources
Other Literature Sources
Research Materials
Miscellaneous