Purification of a new neurotrophic factor from mammalian brain - PubMed (original) (raw)

Purification of a new neurotrophic factor from mammalian brain

Y A Barde et al. EMBO J. 1982.

Abstract

We report the purification from pig brain of a factor supporting the survival of, and fibre outgrowth from, cultured embryonic chick sensory neurons. The purified factor migrates as one single band, mol. wt. 12 300, on gel electrophoresis in the presence of sodium dodecylsulphate (SDS) and is a basic molecule (pI greater than or equal to 10.1). Approximately 1 microgram factor was isolated from 1.5 kg brain. The final degree of purification was estimated to be 1.4 X 10(6)-fold, and the specific activity 0.4 ng/ml/unit, which is similar to that of nerve growth factor (NGF) using the same assay system. This factor is the first neurotrophic factor to be purified since NGF, from which it is clearly distinguished because it has different antigenic and functional properties.

PubMed Disclaimer

Similar articles

• Ciliary neurotrophic factor stimulates neurite outgrowth from spinal cord neurons.
  Oyesiku NM, Wigston DJ. Oyesiku NM, et al. J Comp Neurol. 1996 Jan 1;364(1):68-77. doi: 10.1002/(SICI)1096-9861(19960101)364:1<68::AID-CNE6>3.0.CO;2-Q. J Comp Neurol. 1996. PMID: 8789276
• Purification of adult rat sciatic nerve ciliary neuronotrophic factor.
  Manthorpe M, Skaper SD, Williams LR, Varon S. Manthorpe M, et al. Brain Res. 1986 Mar 5;367(1-2):282-6. doi: 10.1016/0006-8993(86)91603-3. Brain Res. 1986. PMID: 3697702
• Brain-derived proteins that rescue spinal motoneurons from cell death in the chick embryo: comparisons with target-derived and recombinant factors.
  Johnson JE, Wei YQ, Prevette D, Oppenheim RW. Johnson JE, et al. J Neurobiol. 1995 Aug;27(4):573-89. doi: 10.1002/neu.480270411. J Neurobiol. 1995. PMID: 7561835
• Purification and initial characterization of rat B49 glial cell line-derived neurotrophic factor.
  Lin LF, Zhang TJ, Collins F, Armes LG. Lin LF, et al. J Neurochem. 1994 Aug;63(2):758-68. doi: 10.1046/j.1471-4159.1994.63020758.x. J Neurochem. 1994. PMID: 8035200
• Neurotrophic factors and neuronal death.
  Thoenen H, Barde YA, Davies AM, Johnson JE. Thoenen H, et al. Ciba Found Symp. 1987;126:82-95. doi: 10.1002/9780470513422.ch6. Ciba Found Symp. 1987. PMID: 3556091 Review.

Cited by

• Deconstructing brain-derived neurotrophic factor actions in adult brain circuits to bridge an existing informational gap in neuro-cell biology.
  Bowling H, Bhattacharya A, Klann E, Chao MV. Bowling H, et al. Neural Regen Res. 2016 Mar;11(3):363-7. doi: 10.4103/1673-5374.179031. Neural Regen Res. 2016. PMID: 27127458 Free PMC article. Review.
• Custom genotyping for substance addiction susceptibility genes in Jordanians of Arab descent.
  Al-Eitan LN, Jaradat SA, Hulse GK, Tay GK. Al-Eitan LN, et al. BMC Res Notes. 2012 Sep 10;5:497. doi: 10.1186/1756-0500-5-497. BMC Res Notes. 2012. PMID: 22963930 Free PMC article.
• Chondroitinase administration and pcDNA3.1-BDNF-BMSC transplantation promote motor functional recovery associated with NGF expression in spinal cord-transected rat.
  Xiong LL, Li Y, Shang FF, Chen SW, Chen H, Ju SM, Zou Y, Tian HL, Wang TH, Luo CZ, Wang XY. Xiong LL, et al. Spinal Cord. 2016 Dec;54(12):1088-1095. doi: 10.1038/sc.2016.55. Epub 2016 Jun 28. Spinal Cord. 2016. PMID: 27349609
• Fingolimod, a sphingosine-1 phosphate receptor modulator, increases BDNF levels and improves symptoms of a mouse model of Rett syndrome.
  Deogracias R, Yazdani M, Dekkers MP, Guy J, Ionescu MC, Vogt KE, Barde YA. Deogracias R, et al. Proc Natl Acad Sci U S A. 2012 Aug 28;109(35):14230-5. doi: 10.1073/pnas.1206093109. Epub 2012 Aug 13. Proc Natl Acad Sci U S A. 2012. PMID: 22891354 Free PMC article.
• Protective effect of Lactobacillus salivarius Li01 on thioacetamide-induced acute liver injury and hyperammonaemia.
  Yang L, Bian X, Wu W, Lv L, Li Y, Ye J, Jiang X, Wang Q, Shi D, Fang D, Wu J, Wang K, Wang Q, Xia J, Xie J, Lu Y, Li L. Yang L, et al. Microb Biotechnol. 2020 Nov;13(6):1860-1876. doi: 10.1111/1751-7915.13629. Epub 2020 Jul 11. Microb Biotechnol. 2020. PMID: 32652882 Free PMC article.

References

1. 1. Proc Natl Acad Sci U S A. 1969 Oct;64(2):787-94 - PubMed
2. 1. Nature. 1962 Jul 21;195:281-3 - PubMed
3. 1. J Biol Chem. 1971 Jul 25;246(14):4504-9 - PubMed
4. 1. J Biol Chem. 1975 Apr 10;250(7):2515-20 - PubMed
5. 1. J Neurochem. 1976 Jun;26(6):1207-11 - PubMed

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Europe PubMed Central
  • Nature Publishing Group
  • PubMed Central
  • Wiley

• Other Literature Sources

  • The Lens - Patent Citations Database

• Molecular Biology Databases

  • Mouse Genome Informatics (MGI)

• Research Materials

  • NCI CPTC Antibody Characterization Program

• Miscellaneous

  • NCI CPTAC Assay Portal