Insulin-stimulated GLUT4 translocation is mediated by a divergent intracellular signaling pathway - PubMed (original) (raw)
. 1995 Nov 24;270(47):27991-4.
doi: 10.1074/jbc.270.47.27991.
Affiliations
- PMID: 7499278
- DOI: 10.1074/jbc.270.47.27991
Free article
Insulin-stimulated GLUT4 translocation is mediated by a divergent intracellular signaling pathway
T Haruta et al. J Biol Chem. 1995.
Free article
Abstract
Insulin stimulates glucose transport largely by mediating translocation of the insulin-sensitive glucose transporter (GLUT4) from an intracellular compartment to the plasma membrane. Using single cell microinjection of 3T3-L1 adipocytes, coupled with immunofluorescence detection of GLUT4 proteins, we have determined that inhibition of endogenous p21ras or injection of oncogenic p21ras has no effect on insulin-stimulated GLUT4 translocation. On the other hand, microinjection of anti-phosphotyrosine antibodies or inhibition of endogenous phosphatidylinositol 3-kinase by microinjection of a GST-p85 SH2 fusion protein markedly inhibits this biologic effect of insulin. These data suggest that the p21ras/mitogen-activated protein kinase pathway is not involved in this metabolic effect of insulin, whereas tyrosine phosphorylation and stimulation of phosphatidylinositol 3-kinase activity are critical components of this signaling pathway.
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