Cdk-activating kinase complex is a component of human transcription factor TFIIH - PubMed (original) (raw)
. 1995 Mar 16;374(6519):283-7.
doi: 10.1038/374283a0.
Affiliations
- PMID: 7533895
- DOI: 10.1038/374283a0
Cdk-activating kinase complex is a component of human transcription factor TFIIH
R Shiekhattar et al. Nature. 1995.
Abstract
Transcription factor IIH (TFIIH) contains a kinase capable of phosphorylating the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II (RNAPII). Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography. We find that affinity-purified antibodies directed against cyclin H inhibit TFIIH-dependent transcription and that both cyclin H and Cdk7 antibodies inhibit phosphorylation of the CTD of the largest subunit of the RNAPII in the preinitiation complex. Cak is present in at least two distinct complexes, TFIIH and a smaller complex that is unable to phosphorylate RNAPII in the preinitiation complex. Both Cak complexes, as well as recombinant Cak, phosphorylate a CTD peptide. Finally, TFIIH was shown to phosphorylate both Cdc2 and Cdk2, suggesting that there could be a link between transcription and the cell cycle machinery.
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