Phosphatidylinositol 3-kinase encoded by yeast VPS34 gene essential for protein sorting - PubMed (original) (raw)

Comparative Study

. 1993 Apr 2;260(5104):88-91.

doi: 10.1126/science.8385367.

Affiliations

• PMID: 8385367
• DOI: 10.1126/science.8385367

Comparative Study

Phosphatidylinositol 3-kinase encoded by yeast VPS34 gene essential for protein sorting

P V Schu et al. Science. 1993.

Abstract

The VPS34 gene product (Vps34p) is required for protein sorting to the lysosome-like vacuole of the yeast Saccharomyces cerevisiae. Vps34p shares significant sequence similarity with the catalytic subunit of bovine phosphatidylinositol (PI) 3-kinase [the 110-kilodalton (p110) subunit of PI 3-kinase], which is known to interact with activated cell surface receptor tyrosine kinases. Yeast strains deleted for the VPS34 gene or carrying vps34 point mutations lacked detectable PI 3-kinase activity and exhibited severe defects in vacuolar protein sorting. Overexpression of Vps34p resulted in an increase in PI 3-kinase activity, and this activity was specifically precipitated with antisera to Vps34p. VPS34 encodes a yeast PI 3-kinase, and this enzyme appears to regulate intracellular protein trafficking decisions.

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