NARENDRA NYOLA | Rajasthan University of Health Sciences, Jaipur (original) (raw)

Papers by NARENDRA NYOLA

Pharmacognosy Research, 2017

Journal of Biologically Active Products from Nature, 2019

Pharmacognosy research, 2017

Indian traditional system of medicine uses Pithecellobium dulce for the treatment of diabetes mel... more Indian traditional system of medicine uses Pithecellobium dulce for the treatment of diabetes mellitus. This study aims to develop an extract rich in saponins derived from seeds of the plant and to evaluate its antihyperglycemic potential in vitro and in vivo. Defatted seeds were extracted with methanol and processed to afford saponin-enriched fraction (Pithecellobium dulce saponin-enriched fraction [PDSEF]). This fraction was evaluated for its potential to inhibit enzymes such as α-glucosidase and α-amylase, in vitro. The fraction was subjected to oral toxicity study followed by in vivo sucrose tolerance test. An analytical high-performance liquid chromatography method was developed for fingerprinting of the fraction. The method adopted for enrichment of saponins was robust enough to enrich saponin content to 96.37% ±1.21% w/w. PDSEF displayed superior inhibition of enzymes (α-glucosidase and α-amylase with IC50 of 5.12 ± 0.15 μg/ml and 17.28 ± 0.23 μg/ml, respectively) compared to...

A new simple, accurate, precise and reproducible RP-HPLC method has been developed for the simult... more A new simple, accurate, precise and reproducible RP-HPLC method has been developed for the simultaneous estimation of Saxagliptin and Metformin in bulk drug form using C 18 column (Phenomenex, 250 x 4.6 mm, 5 μm) in isocratic mode. The mobile phase consisted of 0.02M Potassium dihydrogen phosphate (KH 2 PO 4), Acetonitrile, Methanol in the ratio of 50:25:25 (v/v/v) at pH 4.3. The detection wavelength was carried out at 240 nm. The method was linear over the concentration range for Saxagliptin 10-50μg/ml and for Metformin 5-25 μg/ml. The recoveries of Saxagliptin and Metformin were found to be 100.48and 101.1% respectively. The validation of method was carried out utilizing ICH-guidelines. The described HPLC method was successfully employed for the analysis of pharmaceutical formulations containing combined dosage form.

Plan: An analytical method for estimation of Methodology: A rapid, sensitive and specific uv Cefi... more Plan: An analytical method for estimation of Methodology: A rapid, sensitive and specific uv Cefixime in tablet dosage form. The method was validated in terms of linearity, accuracy, precision, specificity and limit of quantitation. The optimum conditions for the analysis of the drug were established. The maximum wavelength (of Azithromycin and Cefixime were found to be 235 nm and 288nm respectively. The percentage recovery of Cefixime were 100.28-100.33 and 99.68-100.29 for Azithromycin and 2-10µg/ml for Cefixime 0.0143, r² = 0.9996 and y = 0.0917x + 0.02 accuracy, precision, LOD and LOQ. Outcome: The proposed method was successfully applied for the quantitative determination of tablet dosage form.

An analytical method for estimation of Saxagliptin and Metformin in active pharmaceutical ingride... more An analytical method for estimation of Saxagliptin and Metformin in active pharmaceutical ingrident.The method was validated in terms of linearity, accuracy, precision, specificity, limit of detection and limit of quantitation. The optimum conditions for the analysis of the drug were established. The maximum wavelength (λ max) of Saxagliptin and Metformin were found to be 274 nm and 231 nm respectively. The percentage recovery of Saxagliptin and Metformin were 100.1 and 99.98 respectively. Beer’s laws were obeyed in the concentration range 50-90μg/ml for Saxagliptin and 2-10μg/ml for Metformin. The linear equation for Saxagliptin and Metformin were found to be y = 0.012x- 0.462, r² = 0.987 and y = 0.055x - 0.031, r² = 0.990 respectively. Validation was performed as per ICH guidelines.

Estimation of balofloxacin in API and pharmaceutical formulation by different analytical methods ... more Estimation of balofloxacin in API and pharmaceutical formulation by different analytical methods zero order, first order, second order derivative spectroscopy and RP-HPLC methods. The absorbance value were measure in zero order ,first order, second order derivative spectroscopy and RP-HPLC methods at 289nm, 276nm,300nm and 289nm respectively .Retention time for HPLC was found 4.83 min. Calibration curves were linear between the concentration range of 10-30g/ml, 3-15g/ml ,5-25g/ml, and 10-50g/ml respectively. The % RSD value is less than 2% and the recovery were near 100% for all methods. All the developed methods were applied on tablet formulation and the results were found within the limit as per ICH guideline.

An analytical method for estimation of Metformin and Sitagliptine in bulk and tablet formulation.... more An analytical method for estimation of Metformin and Sitagliptine in bulk and tablet formulation. A rapid, sensitive and specific uv-vis method was developed and validated for the estimation of Metformin and Sitagliptine in tablet dosage form. The method was validated in terms of linearity, accuracy, precision, specificity, limit of detection and limit of quantitation. The optimum conditions for the analysis of the drug were established. The maximum wavelength (λ max) of Metformin and Sitagliptine were found to be 231 nm and 267 nm respectively. The percentage recovery of Metformin and Sitagliptin were 99.4 and 99.78 respectively. Beer's laws were obeyed in the concentration range of 2-10µg/ml for Metformin and 20-60µg/ml for Sitagliptin. The linear equation for Metformin and Sitagliptin were found to be y = 0.055x -0.031, r² = 0.990 and y = 0.007x + 0.089, r² = 0.999 respectively. Validation was performed as ICH guidelines for linearity, accuracy, precision, LOD and LOQ. The pr...

International Journal of Research in Pharmaceutical and Biomedical Sciences

A new simple, accurate, precise and reproducible RP-HPLC method has been developed for the simult... more A new simple, accurate, precise and reproducible RP-HPLC method has been developed for the simultaneous estimation of Sitagliptin and Metformin in bulk and pharmaceutical dosage form using C18 column (Phenomenex, 250 x 4.6 mm, 5 μm) in isocratic mode. The mobile phase consisted of 0.02M potassium dihydrogen phosphate (KH2PO4) and acetonitrile in the ratio of 55:45(v/v) at pH 4.3. The detection was carried out at 252 nm. The method was linear over the concentration range for Sitagliptin 4-20μg/ml and for Metformin 10-50μg/ml. The recoveries of Sitagliptin and Metformin were found to be 100.27 and 100.73% respectively. The validation of method was carried out utilizing ICH-guidelines. The described HPLC method was successfully employed for the analysis of pharmaceutical formulations containing combined dosage form.

A new combination of cefixime and azithromycin are used for the treatment of upper and lower resp... more A new combination of cefixime and azithromycin are used for the treatment of upper and lower respiratory tract infection. A simple, accurate, precise and reproducible RP-HPLC method has been developed for the simultaneous estimation of cefixime and azithromycin in pharmaceutical dosage form .The mobile phase consisted of 0.02M Potassium dihydrogen phosphate (KH 2 PO 4): Acetonitrile in the ratio of 65:35 (v/v). The method obeys Beer's law in the concentration range of 40-60 μg/ml (R 2 = 0.998) for cefixime and 50-70μg/ml (R 2 = 0.999) for azithromycin. The LOD and LOQ were found to be 1.06μg/ml and 3.21μg/ml for cefixime and 2.48μg/ml and 7.50μg/ml for azithromycin respectively. The recovery of cefixime and azithromycin were found to be 99.84% and 100.76% respectively showing accuracy of the method. The method was validated statistically as per ICH guidelines. The method showed good reproducibility and recovery with % RSD less than 2. So, the proposed method was found to be simp...

Plan: Stability indicating RP-HPLC method for the ceftaroline fosamil acetate. Preface: The devel... more Plan: Stability indicating RP-HPLC method for the ceftaroline fosamil acetate. Preface: The developed method is simple, fast and accurate and can be used for routine analysis of Market formulations. Methodology: Stability indicating reverse phase high performance liquid chromatography method was developed and validated for the analysis of ceftaroline fosamil in bulk form. Chromatographic separation was achieved on a X-Bridge shield RP (4.6×100mm, 5µ) column, maintained at 30 0 c with a mobile phase consisting of acetonitrile and ammonium dihydrogen ortho phosphate buffer in the ratio of 10: 90. The mobile phase pumped at a rate of 1 ml/ min. and the detection was carried out at 242.6nm by PDA detector. The retention time was obtained 4.312.The peak area plot was linear over the concentration range of 50µg/ml to 250µg/ml. The different experimental parameters affecting the stability were optimized. The method was validated for accuracy, precision, specificity, robustness, LOD and LOQ in accordance with International Conference on Harmonization (ICH) guidelines. Outcome: The proposed method was successfully applied for the analysis of ceftaroline fosamil in bulk form.

… Research Journal of …

Abstract: The aim of this work was to develop a new uv-vis spectrophotometric method for the dete... more Abstract: The aim of this work was to develop a new uv-vis spectrophotometric method for the determination of Ibuprofen and famotidine in combination dosage form. A simple, selective, accurate spectroscopic method was developed and validated for the analysis of ...

Pharmacognosy Research, 2017

Journal of Biologically Active Products from Nature, 2019

Pharmacognosy research, 2017

Indian traditional system of medicine uses Pithecellobium dulce for the treatment of diabetes mel... more Indian traditional system of medicine uses Pithecellobium dulce for the treatment of diabetes mellitus. This study aims to develop an extract rich in saponins derived from seeds of the plant and to evaluate its antihyperglycemic potential in vitro and in vivo. Defatted seeds were extracted with methanol and processed to afford saponin-enriched fraction (Pithecellobium dulce saponin-enriched fraction [PDSEF]). This fraction was evaluated for its potential to inhibit enzymes such as α-glucosidase and α-amylase, in vitro. The fraction was subjected to oral toxicity study followed by in vivo sucrose tolerance test. An analytical high-performance liquid chromatography method was developed for fingerprinting of the fraction. The method adopted for enrichment of saponins was robust enough to enrich saponin content to 96.37% ±1.21% w/w. PDSEF displayed superior inhibition of enzymes (α-glucosidase and α-amylase with IC50 of 5.12 ± 0.15 μg/ml and 17.28 ± 0.23 μg/ml, respectively) compared to...

A new simple, accurate, precise and reproducible RP-HPLC method has been developed for the simult... more A new simple, accurate, precise and reproducible RP-HPLC method has been developed for the simultaneous estimation of Saxagliptin and Metformin in bulk drug form using C 18 column (Phenomenex, 250 x 4.6 mm, 5 μm) in isocratic mode. The mobile phase consisted of 0.02M Potassium dihydrogen phosphate (KH 2 PO 4), Acetonitrile, Methanol in the ratio of 50:25:25 (v/v/v) at pH 4.3. The detection wavelength was carried out at 240 nm. The method was linear over the concentration range for Saxagliptin 10-50μg/ml and for Metformin 5-25 μg/ml. The recoveries of Saxagliptin and Metformin were found to be 100.48and 101.1% respectively. The validation of method was carried out utilizing ICH-guidelines. The described HPLC method was successfully employed for the analysis of pharmaceutical formulations containing combined dosage form.

Plan: An analytical method for estimation of Methodology: A rapid, sensitive and specific uv Cefi... more Plan: An analytical method for estimation of Methodology: A rapid, sensitive and specific uv Cefixime in tablet dosage form. The method was validated in terms of linearity, accuracy, precision, specificity and limit of quantitation. The optimum conditions for the analysis of the drug were established. The maximum wavelength (of Azithromycin and Cefixime were found to be 235 nm and 288nm respectively. The percentage recovery of Cefixime were 100.28-100.33 and 99.68-100.29 for Azithromycin and 2-10µg/ml for Cefixime 0.0143, r² = 0.9996 and y = 0.0917x + 0.02 accuracy, precision, LOD and LOQ. Outcome: The proposed method was successfully applied for the quantitative determination of tablet dosage form.

An analytical method for estimation of Saxagliptin and Metformin in active pharmaceutical ingride... more An analytical method for estimation of Saxagliptin and Metformin in active pharmaceutical ingrident.The method was validated in terms of linearity, accuracy, precision, specificity, limit of detection and limit of quantitation. The optimum conditions for the analysis of the drug were established. The maximum wavelength (λ max) of Saxagliptin and Metformin were found to be 274 nm and 231 nm respectively. The percentage recovery of Saxagliptin and Metformin were 100.1 and 99.98 respectively. Beer’s laws were obeyed in the concentration range 50-90μg/ml for Saxagliptin and 2-10μg/ml for Metformin. The linear equation for Saxagliptin and Metformin were found to be y = 0.012x- 0.462, r² = 0.987 and y = 0.055x - 0.031, r² = 0.990 respectively. Validation was performed as per ICH guidelines.

Estimation of balofloxacin in API and pharmaceutical formulation by different analytical methods ... more Estimation of balofloxacin in API and pharmaceutical formulation by different analytical methods zero order, first order, second order derivative spectroscopy and RP-HPLC methods. The absorbance value were measure in zero order ,first order, second order derivative spectroscopy and RP-HPLC methods at 289nm, 276nm,300nm and 289nm respectively .Retention time for HPLC was found 4.83 min. Calibration curves were linear between the concentration range of 10-30g/ml, 3-15g/ml ,5-25g/ml, and 10-50g/ml respectively. The % RSD value is less than 2% and the recovery were near 100% for all methods. All the developed methods were applied on tablet formulation and the results were found within the limit as per ICH guideline.

An analytical method for estimation of Metformin and Sitagliptine in bulk and tablet formulation.... more An analytical method for estimation of Metformin and Sitagliptine in bulk and tablet formulation. A rapid, sensitive and specific uv-vis method was developed and validated for the estimation of Metformin and Sitagliptine in tablet dosage form. The method was validated in terms of linearity, accuracy, precision, specificity, limit of detection and limit of quantitation. The optimum conditions for the analysis of the drug were established. The maximum wavelength (λ max) of Metformin and Sitagliptine were found to be 231 nm and 267 nm respectively. The percentage recovery of Metformin and Sitagliptin were 99.4 and 99.78 respectively. Beer's laws were obeyed in the concentration range of 2-10µg/ml for Metformin and 20-60µg/ml for Sitagliptin. The linear equation for Metformin and Sitagliptin were found to be y = 0.055x -0.031, r² = 0.990 and y = 0.007x + 0.089, r² = 0.999 respectively. Validation was performed as ICH guidelines for linearity, accuracy, precision, LOD and LOQ. The pr...

International Journal of Research in Pharmaceutical and Biomedical Sciences

A new simple, accurate, precise and reproducible RP-HPLC method has been developed for the simult... more A new simple, accurate, precise and reproducible RP-HPLC method has been developed for the simultaneous estimation of Sitagliptin and Metformin in bulk and pharmaceutical dosage form using C18 column (Phenomenex, 250 x 4.6 mm, 5 μm) in isocratic mode. The mobile phase consisted of 0.02M potassium dihydrogen phosphate (KH2PO4) and acetonitrile in the ratio of 55:45(v/v) at pH 4.3. The detection was carried out at 252 nm. The method was linear over the concentration range for Sitagliptin 4-20μg/ml and for Metformin 10-50μg/ml. The recoveries of Sitagliptin and Metformin were found to be 100.27 and 100.73% respectively. The validation of method was carried out utilizing ICH-guidelines. The described HPLC method was successfully employed for the analysis of pharmaceutical formulations containing combined dosage form.

A new combination of cefixime and azithromycin are used for the treatment of upper and lower resp... more A new combination of cefixime and azithromycin are used for the treatment of upper and lower respiratory tract infection. A simple, accurate, precise and reproducible RP-HPLC method has been developed for the simultaneous estimation of cefixime and azithromycin in pharmaceutical dosage form .The mobile phase consisted of 0.02M Potassium dihydrogen phosphate (KH 2 PO 4): Acetonitrile in the ratio of 65:35 (v/v). The method obeys Beer's law in the concentration range of 40-60 μg/ml (R 2 = 0.998) for cefixime and 50-70μg/ml (R 2 = 0.999) for azithromycin. The LOD and LOQ were found to be 1.06μg/ml and 3.21μg/ml for cefixime and 2.48μg/ml and 7.50μg/ml for azithromycin respectively. The recovery of cefixime and azithromycin were found to be 99.84% and 100.76% respectively showing accuracy of the method. The method was validated statistically as per ICH guidelines. The method showed good reproducibility and recovery with % RSD less than 2. So, the proposed method was found to be simp...

Plan: Stability indicating RP-HPLC method for the ceftaroline fosamil acetate. Preface: The devel... more Plan: Stability indicating RP-HPLC method for the ceftaroline fosamil acetate. Preface: The developed method is simple, fast and accurate and can be used for routine analysis of Market formulations. Methodology: Stability indicating reverse phase high performance liquid chromatography method was developed and validated for the analysis of ceftaroline fosamil in bulk form. Chromatographic separation was achieved on a X-Bridge shield RP (4.6×100mm, 5µ) column, maintained at 30 0 c with a mobile phase consisting of acetonitrile and ammonium dihydrogen ortho phosphate buffer in the ratio of 10: 90. The mobile phase pumped at a rate of 1 ml/ min. and the detection was carried out at 242.6nm by PDA detector. The retention time was obtained 4.312.The peak area plot was linear over the concentration range of 50µg/ml to 250µg/ml. The different experimental parameters affecting the stability were optimized. The method was validated for accuracy, precision, specificity, robustness, LOD and LOQ in accordance with International Conference on Harmonization (ICH) guidelines. Outcome: The proposed method was successfully applied for the analysis of ceftaroline fosamil in bulk form.

… Research Journal of …

Abstract: The aim of this work was to develop a new uv-vis spectrophotometric method for the dete... more Abstract: The aim of this work was to develop a new uv-vis spectrophotometric method for the determination of Ibuprofen and famotidine in combination dosage form. A simple, selective, accurate spectroscopic method was developed and validated for the analysis of ...