Dr. Akeel H Al-Assie | Tikrit University (original) (raw)

Papers by Dr. Akeel H Al-Assie

Tikrit Journal of Pure Science

300 samples from different infection sourcel for a patients and revisers in Azadi hospital, Kirku... more 300 samples from different infection sourcel for a patients and revisers in Azadi hospital, Kirkuk generic hospital, and children hospital, from various ages for both genders, to isolate Escherichia coli bacteria and diagnosis it, and study the genetic variety which distribution to clinical samples by (140) urine samples, (45) diarrhea samples, (40) wound swab, (40) burn swab, and (35) from otitis media infection. The growth isolation on the different cultural media was diagnosed from the appearance, microscopical, and cultural characteristics. The results the diagnosis showed that (75) clinical isolation about (38.65%) refers to E. coli bacteria distributed by (9) isolation at ratio (31%) from the burn swab, (6) isolation at ratio (26.1) from wound swab, (4) isolation from otitis media infection (19%), (13) isolation from the stool about (40.6%), and (43) isolation (84.3%) from the urinary tract infection (UTI). The sensitivity of the clinical and ecological isolations was studie...

Tikrit Journal of Pure Science

The current study Included the collection of samples (68) grit kidneys of patients undergoing sur... more The current study Included the collection of samples (68) grit kidneys of patients undergoing surgery for lifting gravel in hospitals in the city of Tikrit and for the period from the month of April 2012 to the month of May 2013. The results of the study that people with the proportion of males (64.71%) while she was infected with the proportion of females (35.29 %) outweigh any male female ratio (1.83:1), diagnostic results showed depending on the biochemical tests and API-20system showed that E.coli and Ps.aeruginosa were the most frequent among other bacterial species and by isolating (30.76%) and (21.22%), respectively, while the bacteria P.mirabilis has given isolate percentage (15.38%) Followed by bacteria and Kl.pneumoniae Citrobacter.freundii by insolation (9.6%) and (5.67%) respectively, while giving the rest of the bacterial species (Staph.aureus,, Acinetobacter. freundii, E.faecalis Serratia .marscenes) isolation rate equal (3.84%), while isolating the bacteria Staph.e...

Tikrit Journal of Pure Science

This study aimed to use the Random Amplified Polymorphic DNA (RAPD) markers based on the PCR tech... more This study aimed to use the Random Amplified Polymorphic DNA (RAPD) markers based on the PCR technique in the analysis of genetic variation among seven olive grown cultivar Olea europaea L. cultivars in Saladin province in Iraq Eighteen primers were used in RAPD reactions ,Results of RAPD experiments showed different loci of the bands. The total number of loci defined by the primer was 99 loci, of which 18 were general sites for all samples and 81 different locations. (OPP-01, OPM-15, OPK-13,1700bp) highest, and the lowest partial size in the primers [100 bpOPH-14, OPG-05] The variance between the samples studied was highest, as OPZ-08 (9.0), and less than in OPM-03 (2.1) The ability to discriminate for the primers was characterized by the OPZ-08 primer with a maximum discriminating higher of 9.0. The OPM-03 primer was characterized by the least discriminating of 2.1A total of 429 total bands bundles were produced, of which 18 were general main bands and 411 were polymorphic bands, ...

Tikrit Journal for Agricultural Sciences, 2014

Iraqi Journal of Cancer and Medical Genetics, 2016

Journal of Tikrit University For Agriculture Sciences, 2014

Genotypes of 13 soft wheat (Triticum aestivum) L. were grown in the (Tikrit Meteorological Statio... more Genotypes of 13 soft wheat (Triticum aestivum) L. were grown in the (Tikrit Meteorological Station) during growth season 2017-2018 by planting the seeds in plastic pots. DNA was isolated from the young leaves. PCR reactions were performed using five specialized primers to identify the Dreb1 genes found in the three genomes of wheat (A, B and D). Previous studies have indicated their significant role in giving plants resistance against stress Abiotic stress and in particular drought stress. The P21F / P21R and P25F / PRa primers were designed to detect the downstream and upstream regions of the Dreb1 gene in genome A. The P18F / P18R primer was specifically designed for the Dreb1 gene in genome B. The pair of P20F / P20R and P22F / PRa primers were designed to amplify Dreb1 sequences in genome D. The results were positive with most of the primer interactions where the required bands were found in most genotypes, except for the P21F / P21R primer reactions were negative, Multiplex PCR...

This study includes isolation, purification, and identification of Aspergillus niger from differe... more This study includes isolation, purification, and identification of Aspergillus niger from different sources (soil, Seeds, powdered milk and factory waste water) by traditional methods (macroscopic and microscopically). and study the relationships of genetic among the A. niger isolates based PCR (RAPD) technique (find the DNA fingerprint). The results of this study revealed that genetic diversity and relationships among twenty four isolates were determined by using the random amplified polymorphic DNA (RAPD) technique. Genomic DNA of each isolates was extract at final concentration of (115-959) μg /l per (2-3) gm of wet mycelium, and at a purity (1.7-1.9). Each DNA of isolates were amplified with each 24 primers and the products were resolved electrophoretically on 1.2% agarose gel, stained with ethidium bromide and photographed under UV light. Ten primers failed to amplify DNA of A. niger isolates but the rest 14 primers produced 367 bands, of these bands 99.4% (365) bands were poly...

Research on Crops, 2019

This study aimed at determining the genotoxic effects of different environmental pollutants in Ba... more This study aimed at determining the genotoxic effects of different environmental pollutants in Bazian cement factory in Bazian district, Iraq. Three species of plants viz., Platycladus orientalis, Eucalyptus spp. and Melia azedarach were studied. These plants were collected from areas in and near the cement factory, while the control of this study was one collected from area far from the pollution sources. Atomic Absorption Spectrophotometry (AAS) was used for estimation of the pollution status of some heavy metal concentrations (Pb, Cd, Cr, Ni, Fe, Cu and Zn). Random Amplified Polymorphic DNA (RAPD) revealed new bands and/or disappearing of normal bands in comparison with control samples. In this study, the mean of heavy metal concentrations for all species of plants was below 100 mg/kg and resembled sample results of control. Statistical results for heavy metals in plant leaves indicated significant differences only among sites for most metals. The lowest was level of Genomic Template Stability (GTS) ratios which are related to CCR samples followed by Gate3 and Out. This is in agreement with distances from main stack, respectively, and might have led to a high level of genotoxic effect on three plants' species (68.36, 69.38 and 89.79%; 79.2, 83.16 and 89.1% and 79.12, 87.91 and 99.94), respectively. Different primers (25) were examined. Twenty primers only resulted in reproducible and clear bands. The current study showed that the samples of the selected plants were suitable for the detection of genetic toxicity. The RAPD technique used was suitable for the study of genetic toxicity. The use of plants, which are indicated as biomarker indicator in the early detection of genetic toxicity factors, was highly sensitive in assessing the effect of air pollution.

Journal of University of Anbar for Pure Science

The aim of the present study was the molecular characterization and the evaluation of variability... more The aim of the present study was the molecular characterization and the evaluation of variability and genetic relationship of six Pseudomonas aeruginosa isolates using PCR-based Randomly Amplified Polymorphic DNA (RAPD) technique. A total number of 86 samples were collected from patients that hospitalized in Tikrit Teaching Hospital in Tikrit city. These samples were taken from patients basing on the sources of infections, the isolates were taken from: wounds, ear, burns, urine, sputum, and eyes infections. Using enrichment, selective media, and biochemical tests, that characterized and identified as P. aeruginosa. Genomic DNA was extracted from six P. aeruginosa isolates isolated from these different sources. These genomic DNA samples were found to have a suitable concentration and purity for RAPD-PCR analysis. RAPD-PCR technique was performed using 15 different Operon random primers. Eleven primers gave successful amplification results in repeated experiments. As a result, the total number of amplified bands was 270 and the total number of polymorphic bands was 234. The highest number of polymorphic bands (39 bands) was produced by primer OPX-01. The primer efficiency ranged from 3.70 (primer OPA-11) to 14.44 (primer OPX-01) and the discriminatory value ranged from 1.70% (primer OPA-11) to 16.66% (primer OPX-01). In addition, genetic distance and cluster analysis among different P. aeruginosa isolates were estimated by using UPGMA computer program basing on RAPD-PCR banding patterns that obtained in this study. These results suggesting that possible and frequent occurrence of mutations in DNA sequencing P. aeruginosa bacteria from different sources and locations. This study has proved existence genetic differences (DNA polymorphism) among the six P. aeruginosa isolates isolated from different sources. Therefore, we can say that RAPD technique could be an efficient technique for studying the molecular characterization and the epidemiology of P. aeruginosa bacteria.

Tikrit Journal for Agricultural Sciences

This study was carried out to study some morphological parts of adult male of Liriomyza spp, incl... more This study was carried out to study some morphological parts of adult male of Liriomyza spp, including the antenna, the femur and the middle vein (Cu Al), as well as the shape of mines that larvae made them on the leaves. Results showed that there were two species of L.bryoinae on tomato crop and L.sativae on climbing beans crop, this was agreed with the molecular study that it was conducted using the RAPD Random Amplification of Polymorphic DNA based on the PCR technique to determine genetic variability of DNA that it was extraction from adults and pupae and obtained a quantity of it ranged from (35-556.9) mg and purity ranged between (1.7-2). The RAPD markers were studied using 19 random primers, 13 of which showed a bands and 6 of them didn’t show any bands, the total number of bands loci were 53 loci, 23 of which were main bands and 30 were polymorphism and genetic analysis was performed based on these results. The genetic distance was found and lowest was (0...

Journal for Research in Applied Sciences and Biotechnology

In the current study, the gene expression of FLS gene prefixes was studied in the leaves of the p... more In the current study, the gene expression of FLS gene prefixes was studied in the leaves of the plants of Zingiber officinale, Vitis vinifera, Phoenix dactylifera. This was done using a sample of Artemisia annua leaves as a control sample and using the reference gene 18s rRNA. Relative Quantification gene expression was relied upon as an approved method to extract results. This gene is responsible for encoding Flavonol synthase, which encodes Flavonol compounds in the flavonoid metabolism chain in various plant kingdom plants that perform multiple plant functions as well as directly related to human health. Ginger Z. officinale was proved to have the highest gene expression at CT= 31.6, while V. vinifera and date palm P. dactylifera followed respectively with values 33.78 and 38.31.

Journal of University of Anbar for Pure Science

Familial Hypercholesterolemia (FH) is a genetic disorder, an expression of a defect in the gene t... more Familial Hypercholesterolemia (FH) is a genetic disorder, an expression of a defect in the gene that is responsible for the production of LDL-C receptor. The current study was designed to determine the FH patients in the city of Sulaymaniyah for both sexes. The study included 213 samples, the number of males was 99 and the number of females was 114, both sexes ranged from (30-79) years. The results of cholesterol, triglyceride, high density lipoprotein (HDL-C), and lowdensity lipoprotein (LDL-C) in patients with hypercholesterolemia showed highly significant (P ˂ 0.01) for cholesterol and triglyceride in patients with compared healthy people. (HDL-C and LDL-C) showed a significant decrease (P ˂ 0.01) in the concentration of HDL-C in patients with hypercholesterolemia compared to their level of concentration in healthy individuals, in relation to low-density lipoprotein (LDL-C)-C is the other (P ˂ 0.01) in the concentration level in patients with hypercholesterolemia compared to the level of concentration of healthy. As concerns the effect of hypercholesterolemia on coronary heart disease, the results showed a significant increase (P ˂ 0.01) in cholesterol, triglycerides and LDL-C with a significant decrease in HDL-C concentration in people with heart disease compared with healthy people.

College Of Basic Education Research Journal

Seed treatment with a DBD isolation barrier vacuum reactor. Using the drainage of the insulating ... more Seed treatment with a DBD isolation barrier vacuum reactor. Using the drainage of the insulating barrier in the air at atmospheric pressure and room temperature, the effect of non-thermal plasma treatment of seeds on the development rate of seven broccoli cultivars cultivated in Mosul (Al Rashidiya) was investigated. The use of DBD at atmospheric pressure had a significant influence on germination and plant growth of the seven cultivars of broccoli from the seeds used for a period of 0.5, 1, 3, 5, 10, 15 minutes as compared to the control group. In comparison to the

IRAQI JOURNAL OF AGRICULTURAL SCIENCES, Dec 1, 2018

This study was carried out to assess impacts of industrial activities on environmental quality in... more This study was carried out to assess impacts of industrial activities on environmental quality in Bazian cement factory, Sulaimani. Environmental impact of factory was carried out from September 2015 to August 2016 at studied area located at north west of city with coordinates of 35º36'242"-045º04'470".Pollutant particles can have as consequence reduction of biodiversity and quality of water, soil and whole ecosystems. Especially, cement dust can be emitted at every stage of cement production which affects on photosynthetic process, leaf stomata, discoloration, enzymatic malfunction, growth reduction and productivity of plants. In this study, water, soil, air and three plants (Platycladus orientalis , Eucalyptus spp. and Melia azedarach) samples were collected. Ten water sources including 6 wells, 3 tanks and control in and around factory were selected in order to evaluate some physicochemical characteristics of water. Concentrations of some heavy metals including cadmium, chromium, lead, copper, nickel, iron and zinc were determined to evaluate pollution status. Soil samples include twelve different sites nine of them are cement dust exposed with three control sites. Same work for them had done. Heavy metal analysis and chlorophyll content for plants also determined. Air gases results had taken online from computer fixed on main stack monthly. Also dust from main stack had collected and analyzed for physicochemical parameters and heavy metals. Results showed that were within normal standards.

The study aimed at diagnostic FMV virus from infected Ficus carica from some Saladin nurseries ba... more The study aimed at diagnostic FMV virus from infected Ficus carica from some Saladin nurseries based on the technique of Reverse Transcription RT-PCR. A band 300 bp was given. The result showed FMV similar to that found in Costa Rica, Iran, Lurstan, Japan, and also gave a band of 367pb of FMV RNA1 and the absence of other species. FLMAV-1 isolation was detected in fig leaf with a 352pb band and is similar in some countries. The study also included the use of Bacillus subtilis, Spirulina platensis, Ganoderma lucidum, (G. lucidum +S. platensis), (S. platensis+B. subtilis), (G. lucidum+B. subtilis). The treatment of B. subtilis + S. platensis for yellow and black to study the percentage of the severity of the effect of the injury which is the lowest and reached 23.0% compared with a control treatment, which gave 99.6%. The results of chlorophyll the treatment of mushrooms with moss (G + Sp) with a value of Spad 43.15 which is the highest compared to the treatment of control amounted to 30.77 Spad.

Asia Pacific Journal of Molecular Biology and Biotechnology, 2021

Short tandem repeats (STRs) have been recommended as the highest polymorphic loci among the human... more Short tandem repeats (STRs) have been recommended as the highest polymorphic loci among the humana DNA regions. Therefore, STRs are agreeable to many genetic fields like forensic, population genetics and anthropological studies. The main aim of this research is to evaluate the autosomal STRs in Tikrit city-Iraq, to expand the human genetics database and forensic genetics analysis. The DNA database was obtained from 306 unrelated volunteers from native Tikrit population-Iraq, using 15 autosomal STR loci. The current study determined the allele frequencies in the Tikrit population and then compared them with other national Iraqi populations as well as with populations in the Middle East, Africa, and Europe. The highest level of heterozygosity was observed in D8S1179 and TH01 loci (0.797), while the less level was shown by CSF1PO (0.48). The departure from HWE Equilibrium was recorded in only 3 STR loci from a total of 15 loci analyzed (p<0.003). The Combined Match Probability (CMP)...

International Journal for Research in Applied Sciences and Biotechnology, 2021

Introns qualify as Noncoding nucleotide sequences. In splicing, some segments of the RNA transcri... more Introns qualify as Noncoding nucleotide sequences. In splicing, some segments of the RNA transcript (introns) are eliminated, the other segments (exons) are joining together in the formation of the coding RNAs (mRNA, rRNA and tRNA). Also, Non-coding RNA genes are parts of the intronic. On average, there are 7.8 introns and 8.8 exons per human gene. Single nucleotide polymorphisms (SNPs) are existed in the various positions through the human gene, promoters, alternating regions of exons and introns, terminator, in addition to UTRs, untranslated regions (5'- and 3'-).Therefore, many diseases have been associated with SNPs through different mechanisms. In the current review, we will discuss the several genetic and epigenetic regulations included in identifying disease susceptibility linked to numerous SNPs existing in the intronic region.

Kirkuk University Journal-Scientific Studies, 2015

This study was performed on ten varieties of Iraqi dates palm (Barhi, kiara Hamra, sugary, Zuhdi,... more This study was performed on ten varieties of Iraqi dates palm (Barhi, kiara Hamra, sugary, Zuhdi, Khstawi, Khadrawi, Tbrzel, Sayer (osta omran), Prem, Maktoum) to devise specific DNA finger print for a given class using three specialized primers within the SSR markers and then use three restricted enzymes within PCR-RFLP technique to reach the goal. one band was result from all varieties with molecular size 320bp for mpdIRD28 and 200bp for mpdIRD46 , mpdIRD01 locus respectively after performing the PCR reaction within SSR markers. The PCR-RFLP techniqae was used with three restricted enzymes HinfI, TaqI, EcoRI The results reveals the presence or absence of Restriction sites for hybrid alleles (haplotypes) in date palm.

RESEARCH JOURNAL OF PHARMACY AND TECHNOLOGY, 2020

Live attenuated Brucella melitensis Rev.1 strain is currently used in Iraq against caprine and ov... more Live attenuated Brucella melitensis Rev.1 strain is currently used in Iraq against caprine and ovine brucellosis. Vaccination with Rev.1 may lead to the adverse effects of strain persistence in the vaccinated animals and its occasionally spread horizontally. Moreover, in some countries it was shown that the vaccine strain caused human infection, demonstrating the zoonotic hazards of its virulence. Thirty Sheep milk samples and 30 Goat milk samples were taken from animals given a positive result with rose Bengal test. Also 12 Vaginal swabs from aborted ewes and 20 Vaginal swabs from aborted goats were taken in period didn't exceed 10 days after the abortion. At the same time, 50 blood samples taken from patients with Malta fever. The samples cultured on trypton soya broth and then sub-cultured on Brucella Basel agar. Biochemical test, PCR-RELP, and DNA sequencing were used to distinguish Vaccine Rev.1 strain from field strains. Out of 92 samples from goats and sheep, Brucella isolated from 43 (46.7%) case. The results revealed that vaginal discharges are better than milk samples for isolation of the Brucella. In human, the bacteria isolated from 11 (22%) of Malta fever patients. PCR-RELP and DNA sequencing revealed that Rev.1 strain isolated from 8 (21%) animals and 1 (11.1%) of human patients.

Tikrit Journal of Pure Science

300 samples from different infection sourcel for a patients and revisers in Azadi hospital, Kirku... more 300 samples from different infection sourcel for a patients and revisers in Azadi hospital, Kirkuk generic hospital, and children hospital, from various ages for both genders, to isolate Escherichia coli bacteria and diagnosis it, and study the genetic variety which distribution to clinical samples by (140) urine samples, (45) diarrhea samples, (40) wound swab, (40) burn swab, and (35) from otitis media infection. The growth isolation on the different cultural media was diagnosed from the appearance, microscopical, and cultural characteristics. The results the diagnosis showed that (75) clinical isolation about (38.65%) refers to E. coli bacteria distributed by (9) isolation at ratio (31%) from the burn swab, (6) isolation at ratio (26.1) from wound swab, (4) isolation from otitis media infection (19%), (13) isolation from the stool about (40.6%), and (43) isolation (84.3%) from the urinary tract infection (UTI). The sensitivity of the clinical and ecological isolations was studie...

Tikrit Journal of Pure Science

The current study Included the collection of samples (68) grit kidneys of patients undergoing sur... more The current study Included the collection of samples (68) grit kidneys of patients undergoing surgery for lifting gravel in hospitals in the city of Tikrit and for the period from the month of April 2012 to the month of May 2013. The results of the study that people with the proportion of males (64.71%) while she was infected with the proportion of females (35.29 %) outweigh any male female ratio (1.83:1), diagnostic results showed depending on the biochemical tests and API-20system showed that E.coli and Ps.aeruginosa were the most frequent among other bacterial species and by isolating (30.76%) and (21.22%), respectively, while the bacteria P.mirabilis has given isolate percentage (15.38%) Followed by bacteria and Kl.pneumoniae Citrobacter.freundii by insolation (9.6%) and (5.67%) respectively, while giving the rest of the bacterial species (Staph.aureus,, Acinetobacter. freundii, E.faecalis Serratia .marscenes) isolation rate equal (3.84%), while isolating the bacteria Staph.e...

Tikrit Journal of Pure Science

This study aimed to use the Random Amplified Polymorphic DNA (RAPD) markers based on the PCR tech... more This study aimed to use the Random Amplified Polymorphic DNA (RAPD) markers based on the PCR technique in the analysis of genetic variation among seven olive grown cultivar Olea europaea L. cultivars in Saladin province in Iraq Eighteen primers were used in RAPD reactions ,Results of RAPD experiments showed different loci of the bands. The total number of loci defined by the primer was 99 loci, of which 18 were general sites for all samples and 81 different locations. (OPP-01, OPM-15, OPK-13,1700bp) highest, and the lowest partial size in the primers [100 bpOPH-14, OPG-05] The variance between the samples studied was highest, as OPZ-08 (9.0), and less than in OPM-03 (2.1) The ability to discriminate for the primers was characterized by the OPZ-08 primer with a maximum discriminating higher of 9.0. The OPM-03 primer was characterized by the least discriminating of 2.1A total of 429 total bands bundles were produced, of which 18 were general main bands and 411 were polymorphic bands, ...

Tikrit Journal for Agricultural Sciences, 2014

Iraqi Journal of Cancer and Medical Genetics, 2016

Journal of Tikrit University For Agriculture Sciences, 2014

Genotypes of 13 soft wheat (Triticum aestivum) L. were grown in the (Tikrit Meteorological Statio... more Genotypes of 13 soft wheat (Triticum aestivum) L. were grown in the (Tikrit Meteorological Station) during growth season 2017-2018 by planting the seeds in plastic pots. DNA was isolated from the young leaves. PCR reactions were performed using five specialized primers to identify the Dreb1 genes found in the three genomes of wheat (A, B and D). Previous studies have indicated their significant role in giving plants resistance against stress Abiotic stress and in particular drought stress. The P21F / P21R and P25F / PRa primers were designed to detect the downstream and upstream regions of the Dreb1 gene in genome A. The P18F / P18R primer was specifically designed for the Dreb1 gene in genome B. The pair of P20F / P20R and P22F / PRa primers were designed to amplify Dreb1 sequences in genome D. The results were positive with most of the primer interactions where the required bands were found in most genotypes, except for the P21F / P21R primer reactions were negative, Multiplex PCR...

This study includes isolation, purification, and identification of Aspergillus niger from differe... more This study includes isolation, purification, and identification of Aspergillus niger from different sources (soil, Seeds, powdered milk and factory waste water) by traditional methods (macroscopic and microscopically). and study the relationships of genetic among the A. niger isolates based PCR (RAPD) technique (find the DNA fingerprint). The results of this study revealed that genetic diversity and relationships among twenty four isolates were determined by using the random amplified polymorphic DNA (RAPD) technique. Genomic DNA of each isolates was extract at final concentration of (115-959) μg /l per (2-3) gm of wet mycelium, and at a purity (1.7-1.9). Each DNA of isolates were amplified with each 24 primers and the products were resolved electrophoretically on 1.2% agarose gel, stained with ethidium bromide and photographed under UV light. Ten primers failed to amplify DNA of A. niger isolates but the rest 14 primers produced 367 bands, of these bands 99.4% (365) bands were poly...

Research on Crops, 2019

This study aimed at determining the genotoxic effects of different environmental pollutants in Ba... more This study aimed at determining the genotoxic effects of different environmental pollutants in Bazian cement factory in Bazian district, Iraq. Three species of plants viz., Platycladus orientalis, Eucalyptus spp. and Melia azedarach were studied. These plants were collected from areas in and near the cement factory, while the control of this study was one collected from area far from the pollution sources. Atomic Absorption Spectrophotometry (AAS) was used for estimation of the pollution status of some heavy metal concentrations (Pb, Cd, Cr, Ni, Fe, Cu and Zn). Random Amplified Polymorphic DNA (RAPD) revealed new bands and/or disappearing of normal bands in comparison with control samples. In this study, the mean of heavy metal concentrations for all species of plants was below 100 mg/kg and resembled sample results of control. Statistical results for heavy metals in plant leaves indicated significant differences only among sites for most metals. The lowest was level of Genomic Template Stability (GTS) ratios which are related to CCR samples followed by Gate3 and Out. This is in agreement with distances from main stack, respectively, and might have led to a high level of genotoxic effect on three plants' species (68.36, 69.38 and 89.79%; 79.2, 83.16 and 89.1% and 79.12, 87.91 and 99.94), respectively. Different primers (25) were examined. Twenty primers only resulted in reproducible and clear bands. The current study showed that the samples of the selected plants were suitable for the detection of genetic toxicity. The RAPD technique used was suitable for the study of genetic toxicity. The use of plants, which are indicated as biomarker indicator in the early detection of genetic toxicity factors, was highly sensitive in assessing the effect of air pollution.

Journal of University of Anbar for Pure Science

The aim of the present study was the molecular characterization and the evaluation of variability... more The aim of the present study was the molecular characterization and the evaluation of variability and genetic relationship of six Pseudomonas aeruginosa isolates using PCR-based Randomly Amplified Polymorphic DNA (RAPD) technique. A total number of 86 samples were collected from patients that hospitalized in Tikrit Teaching Hospital in Tikrit city. These samples were taken from patients basing on the sources of infections, the isolates were taken from: wounds, ear, burns, urine, sputum, and eyes infections. Using enrichment, selective media, and biochemical tests, that characterized and identified as P. aeruginosa. Genomic DNA was extracted from six P. aeruginosa isolates isolated from these different sources. These genomic DNA samples were found to have a suitable concentration and purity for RAPD-PCR analysis. RAPD-PCR technique was performed using 15 different Operon random primers. Eleven primers gave successful amplification results in repeated experiments. As a result, the total number of amplified bands was 270 and the total number of polymorphic bands was 234. The highest number of polymorphic bands (39 bands) was produced by primer OPX-01. The primer efficiency ranged from 3.70 (primer OPA-11) to 14.44 (primer OPX-01) and the discriminatory value ranged from 1.70% (primer OPA-11) to 16.66% (primer OPX-01). In addition, genetic distance and cluster analysis among different P. aeruginosa isolates were estimated by using UPGMA computer program basing on RAPD-PCR banding patterns that obtained in this study. These results suggesting that possible and frequent occurrence of mutations in DNA sequencing P. aeruginosa bacteria from different sources and locations. This study has proved existence genetic differences (DNA polymorphism) among the six P. aeruginosa isolates isolated from different sources. Therefore, we can say that RAPD technique could be an efficient technique for studying the molecular characterization and the epidemiology of P. aeruginosa bacteria.

Tikrit Journal for Agricultural Sciences

This study was carried out to study some morphological parts of adult male of Liriomyza spp, incl... more This study was carried out to study some morphological parts of adult male of Liriomyza spp, including the antenna, the femur and the middle vein (Cu Al), as well as the shape of mines that larvae made them on the leaves. Results showed that there were two species of L.bryoinae on tomato crop and L.sativae on climbing beans crop, this was agreed with the molecular study that it was conducted using the RAPD Random Amplification of Polymorphic DNA based on the PCR technique to determine genetic variability of DNA that it was extraction from adults and pupae and obtained a quantity of it ranged from (35-556.9) mg and purity ranged between (1.7-2). The RAPD markers were studied using 19 random primers, 13 of which showed a bands and 6 of them didn’t show any bands, the total number of bands loci were 53 loci, 23 of which were main bands and 30 were polymorphism and genetic analysis was performed based on these results. The genetic distance was found and lowest was (0...

Journal for Research in Applied Sciences and Biotechnology

In the current study, the gene expression of FLS gene prefixes was studied in the leaves of the p... more In the current study, the gene expression of FLS gene prefixes was studied in the leaves of the plants of Zingiber officinale, Vitis vinifera, Phoenix dactylifera. This was done using a sample of Artemisia annua leaves as a control sample and using the reference gene 18s rRNA. Relative Quantification gene expression was relied upon as an approved method to extract results. This gene is responsible for encoding Flavonol synthase, which encodes Flavonol compounds in the flavonoid metabolism chain in various plant kingdom plants that perform multiple plant functions as well as directly related to human health. Ginger Z. officinale was proved to have the highest gene expression at CT= 31.6, while V. vinifera and date palm P. dactylifera followed respectively with values 33.78 and 38.31.

Journal of University of Anbar for Pure Science

Familial Hypercholesterolemia (FH) is a genetic disorder, an expression of a defect in the gene t... more Familial Hypercholesterolemia (FH) is a genetic disorder, an expression of a defect in the gene that is responsible for the production of LDL-C receptor. The current study was designed to determine the FH patients in the city of Sulaymaniyah for both sexes. The study included 213 samples, the number of males was 99 and the number of females was 114, both sexes ranged from (30-79) years. The results of cholesterol, triglyceride, high density lipoprotein (HDL-C), and lowdensity lipoprotein (LDL-C) in patients with hypercholesterolemia showed highly significant (P ˂ 0.01) for cholesterol and triglyceride in patients with compared healthy people. (HDL-C and LDL-C) showed a significant decrease (P ˂ 0.01) in the concentration of HDL-C in patients with hypercholesterolemia compared to their level of concentration in healthy individuals, in relation to low-density lipoprotein (LDL-C)-C is the other (P ˂ 0.01) in the concentration level in patients with hypercholesterolemia compared to the level of concentration of healthy. As concerns the effect of hypercholesterolemia on coronary heart disease, the results showed a significant increase (P ˂ 0.01) in cholesterol, triglycerides and LDL-C with a significant decrease in HDL-C concentration in people with heart disease compared with healthy people.

College Of Basic Education Research Journal

Seed treatment with a DBD isolation barrier vacuum reactor. Using the drainage of the insulating ... more Seed treatment with a DBD isolation barrier vacuum reactor. Using the drainage of the insulating barrier in the air at atmospheric pressure and room temperature, the effect of non-thermal plasma treatment of seeds on the development rate of seven broccoli cultivars cultivated in Mosul (Al Rashidiya) was investigated. The use of DBD at atmospheric pressure had a significant influence on germination and plant growth of the seven cultivars of broccoli from the seeds used for a period of 0.5, 1, 3, 5, 10, 15 minutes as compared to the control group. In comparison to the

IRAQI JOURNAL OF AGRICULTURAL SCIENCES, Dec 1, 2018

This study was carried out to assess impacts of industrial activities on environmental quality in... more This study was carried out to assess impacts of industrial activities on environmental quality in Bazian cement factory, Sulaimani. Environmental impact of factory was carried out from September 2015 to August 2016 at studied area located at north west of city with coordinates of 35º36'242"-045º04'470".Pollutant particles can have as consequence reduction of biodiversity and quality of water, soil and whole ecosystems. Especially, cement dust can be emitted at every stage of cement production which affects on photosynthetic process, leaf stomata, discoloration, enzymatic malfunction, growth reduction and productivity of plants. In this study, water, soil, air and three plants (Platycladus orientalis , Eucalyptus spp. and Melia azedarach) samples were collected. Ten water sources including 6 wells, 3 tanks and control in and around factory were selected in order to evaluate some physicochemical characteristics of water. Concentrations of some heavy metals including cadmium, chromium, lead, copper, nickel, iron and zinc were determined to evaluate pollution status. Soil samples include twelve different sites nine of them are cement dust exposed with three control sites. Same work for them had done. Heavy metal analysis and chlorophyll content for plants also determined. Air gases results had taken online from computer fixed on main stack monthly. Also dust from main stack had collected and analyzed for physicochemical parameters and heavy metals. Results showed that were within normal standards.

The study aimed at diagnostic FMV virus from infected Ficus carica from some Saladin nurseries ba... more The study aimed at diagnostic FMV virus from infected Ficus carica from some Saladin nurseries based on the technique of Reverse Transcription RT-PCR. A band 300 bp was given. The result showed FMV similar to that found in Costa Rica, Iran, Lurstan, Japan, and also gave a band of 367pb of FMV RNA1 and the absence of other species. FLMAV-1 isolation was detected in fig leaf with a 352pb band and is similar in some countries. The study also included the use of Bacillus subtilis, Spirulina platensis, Ganoderma lucidum, (G. lucidum +S. platensis), (S. platensis+B. subtilis), (G. lucidum+B. subtilis). The treatment of B. subtilis + S. platensis for yellow and black to study the percentage of the severity of the effect of the injury which is the lowest and reached 23.0% compared with a control treatment, which gave 99.6%. The results of chlorophyll the treatment of mushrooms with moss (G + Sp) with a value of Spad 43.15 which is the highest compared to the treatment of control amounted to 30.77 Spad.

Asia Pacific Journal of Molecular Biology and Biotechnology, 2021

Short tandem repeats (STRs) have been recommended as the highest polymorphic loci among the human... more Short tandem repeats (STRs) have been recommended as the highest polymorphic loci among the humana DNA regions. Therefore, STRs are agreeable to many genetic fields like forensic, population genetics and anthropological studies. The main aim of this research is to evaluate the autosomal STRs in Tikrit city-Iraq, to expand the human genetics database and forensic genetics analysis. The DNA database was obtained from 306 unrelated volunteers from native Tikrit population-Iraq, using 15 autosomal STR loci. The current study determined the allele frequencies in the Tikrit population and then compared them with other national Iraqi populations as well as with populations in the Middle East, Africa, and Europe. The highest level of heterozygosity was observed in D8S1179 and TH01 loci (0.797), while the less level was shown by CSF1PO (0.48). The departure from HWE Equilibrium was recorded in only 3 STR loci from a total of 15 loci analyzed (p<0.003). The Combined Match Probability (CMP)...

International Journal for Research in Applied Sciences and Biotechnology, 2021

Introns qualify as Noncoding nucleotide sequences. In splicing, some segments of the RNA transcri... more Introns qualify as Noncoding nucleotide sequences. In splicing, some segments of the RNA transcript (introns) are eliminated, the other segments (exons) are joining together in the formation of the coding RNAs (mRNA, rRNA and tRNA). Also, Non-coding RNA genes are parts of the intronic. On average, there are 7.8 introns and 8.8 exons per human gene. Single nucleotide polymorphisms (SNPs) are existed in the various positions through the human gene, promoters, alternating regions of exons and introns, terminator, in addition to UTRs, untranslated regions (5'- and 3'-).Therefore, many diseases have been associated with SNPs through different mechanisms. In the current review, we will discuss the several genetic and epigenetic regulations included in identifying disease susceptibility linked to numerous SNPs existing in the intronic region.

Kirkuk University Journal-Scientific Studies, 2015

This study was performed on ten varieties of Iraqi dates palm (Barhi, kiara Hamra, sugary, Zuhdi,... more This study was performed on ten varieties of Iraqi dates palm (Barhi, kiara Hamra, sugary, Zuhdi, Khstawi, Khadrawi, Tbrzel, Sayer (osta omran), Prem, Maktoum) to devise specific DNA finger print for a given class using three specialized primers within the SSR markers and then use three restricted enzymes within PCR-RFLP technique to reach the goal. one band was result from all varieties with molecular size 320bp for mpdIRD28 and 200bp for mpdIRD46 , mpdIRD01 locus respectively after performing the PCR reaction within SSR markers. The PCR-RFLP techniqae was used with three restricted enzymes HinfI, TaqI, EcoRI The results reveals the presence or absence of Restriction sites for hybrid alleles (haplotypes) in date palm.

RESEARCH JOURNAL OF PHARMACY AND TECHNOLOGY, 2020

Live attenuated Brucella melitensis Rev.1 strain is currently used in Iraq against caprine and ov... more Live attenuated Brucella melitensis Rev.1 strain is currently used in Iraq against caprine and ovine brucellosis. Vaccination with Rev.1 may lead to the adverse effects of strain persistence in the vaccinated animals and its occasionally spread horizontally. Moreover, in some countries it was shown that the vaccine strain caused human infection, demonstrating the zoonotic hazards of its virulence. Thirty Sheep milk samples and 30 Goat milk samples were taken from animals given a positive result with rose Bengal test. Also 12 Vaginal swabs from aborted ewes and 20 Vaginal swabs from aborted goats were taken in period didn't exceed 10 days after the abortion. At the same time, 50 blood samples taken from patients with Malta fever. The samples cultured on trypton soya broth and then sub-cultured on Brucella Basel agar. Biochemical test, PCR-RELP, and DNA sequencing were used to distinguish Vaccine Rev.1 strain from field strains. Out of 92 samples from goats and sheep, Brucella isolated from 43 (46.7%) case. The results revealed that vaginal discharges are better than milk samples for isolation of the Brucella. In human, the bacteria isolated from 11 (22%) of Malta fever patients. PCR-RELP and DNA sequencing revealed that Rev.1 strain isolated from 8 (21%) animals and 1 (11.1%) of human patients.