Renato Santos | UFMG - The Federal University of Minas Gerais (original) (raw)

Papers by Renato Santos

Arquivo Brasileiro De Medicina Veterinaria E Zootecnia, Jul 1, 2023

Chronic lead intoxication in a jaguar (Panthera onca) shot with round lead pelletscase report [In... more Chronic lead intoxication in a jaguar (Panthera onca) shot with round lead pelletscase report [Intoxicação crônica por chumbo em uma onça pintada (Panthera onca) alvejada com projéteis de chumborelato de caso]

Vaccines

Visceral leishmaniasis (VL) is a fatal disease caused by the protozoa Leishmania infantum for whi... more Visceral leishmaniasis (VL) is a fatal disease caused by the protozoa Leishmania infantum for which dogs are the main reservoirs. A vaccine against canine visceral leishmaniasis (CVL) could be an important tool in the control of human and CVL by reducing the infection pressure of L. infantum. Despite the CVL vaccine available on the market, the Brazilian Ministry of Health did not implement the use of it in their control programs. In this sense, there is an urgent need to develop more efficient vaccines. In this study, the association between two polymeric nanoformulations, (poly (D, L-lactic) acid (PLA) polymer) loading Leishmania amazonensis antigens, was evaluated as a potential immunobiological agent against VL using golden hamsters as an experimental model. The results indicated that no significant adverse reactions were observed in animals vaccinated with LAPSmP. LAPSmP presented similar levels of total anti-Leishmania IgG as compared to LAPSmG. The LAPSmP and LAPSmG groups sh...

Vaccines

Leishmaniasis is a widespread vector-borne disease in Brazil, with Leishmania (Leishmania) infant... more Leishmaniasis is a widespread vector-borne disease in Brazil, with Leishmania (Leishmania) infantum as the primary etiological agent of visceral leishmaniasis (VL). Dogs are considered the main reservoir of this parasite, whose treatment in Brazil is restricted to the use of veterinary medicines, which do not promote a parasitological cure. Therefore, efficient vaccine development is the best approach to Canine Visceral Leishmaniasis (CVL) control. With this in mind, this study used hamsters (Mesocricetus auratus) as an experimental model in an anti-Leishmania preclinical vaccine trial to evaluate the safety, antigenicity, humoral response, and effects on tissue parasite load. Two novel formulations of nanoparticles made from poly(D, L-lactic) acid (PLA) polymer loading Leishmania braziliensis crude antigen (LB) exhibiting two different particle sizes were utilized: LBPSmG (570 nm) and LBPSmP (388 nm). The results showed that the nanoparticles were safe and harmless to hamsters and ...

PLOS Neglected Tropical Diseases

Toxoplasmosis is an important zoonotic disease that affects a wide range of warm-blooded host spe... more Toxoplasmosis is an important zoonotic disease that affects a wide range of warm-blooded host species. Neotropical primates (New World Primates; NWP) are highly susceptible, developing a lethal acute systemic disease. Toxoplasmosis in free-ranging NWP is poorly described, with only a few studies based on serosurveys. Herein we performed a retrospective study focusing on the epidemiology and pathology of toxoplasmosis among 1,001 free-ranging marmoset (Callithrix spp.) deaths from the Brazilian Atlantic Forest. This study included marmosets necropsied at the Instituto Municipal de Medicina Veterinária Jorge Vaitsman (IJV) from January 2017 to July 2019, which were found dead from all regions in the State of Rio de Janeiro. Histopathology, immunohistochemistry, and transmission electron microscopy were performed to better characterize toxoplasmosis in this free-ranging population. All samples were also tested for Yellow Fever Virus (YFV) RT-qPCR by the official diagnostic service. A t...

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

Porcine respiratory disease complex is a major health concern for the porcine industry, causing s... more Porcine respiratory disease complex is a major health concern for the porcine industry, causing significant economic loss. In this study, a total of 156 samples from pigs referred to a diagnostic laboratory in Brazil for 15 months were analyzed by histopathology, bacterial isolation, PCR, and immunohistochemistry. Multiple infections were common, so 42.3% of the pigs had more than one pathogen detected in the lungs. Swine influenza virus was detected in 25.0% of the cases. Porcine circovirus type 2 was detected in 7.1% of the pigs, which was often associated with Pasteurella multocida. In addition, one case of porcine circovirus type 3 infection associated with granulomatous pneumonia was diagnosed. Bacteria were isolated in 125 cases, namely Pasteurella multocida (34.0%), Glaesserella (Haemophilus) parasuis (35.2%), Streptococcus suis (13.5%), and Actinobacillus pleuropneumoniae (7.7%). Mycoplasma hyopneumoniae was identified in 7.0% of the cases, and 18.6% of pigs carried Salmonel...

Pesquisa Veterinária Brasileira, 2017

ABSTRACT: This study evaluated the antimicrobial activity and acute or chronic toxicity of the ex... more ABSTRACT: This study evaluated the antimicrobial activity and acute or chronic toxicity of the extract of Stryphnodendron adstringens. The stem bark dry extract was obtained by static maceration with ethanol. Quantification of tannins was performed by the Folin-Denis method, which indicated a total tannin content of 32.7%. The antimicrobial activity of the dry extract of S. adstringens was evaluated by agar-based disk diffusion assay with Escherichia coli (ATCC 25922) and Staphylococcus aureus (ATCC 25923) in the concentration of 200, 400 and 600μL/mL. The results indicated that 600μL/mL inhibited microbial growth, i.e. had antimicrobial activity against these species. Acute and chronic toxic effects of S. adstringens was evaluated in Wistar rats treated with 200, 400, 600 and 800mg/kg of extract, administrated by gavage. Liver degeneration was observed in the group of rats receiving 800mg/kg in chronic exposure, what may indicate some degree of toxicity at this concentration. Howev...

Brazilian Journal of Veterinary Pathology, 2016

This study was based on analysis of the central nervous system from cattle that died after develo... more This study was based on analysis of the central nervous system from cattle that died after developing neurological disease in the State of Minas Gerais from 2004 to 2010. Samples were analyzed by histopathology, histochemistry, and anti- Listeria monocytogenes immunohistochemistry. The frequency of positivity for the techniques used and distribution were determined, and the results were analyzed by Fisher's exact test. Microscopic changes were observed in 29.5% (290/982) of bovine central nervous system samples examined. Inflammatory changes were the most frequent lesions (87.2% - 253/290) with 80% of cases (232/290) presenting non suppurative inflammation. Through histopathologic analyses, presumptive or conclusive etiology was obtained in 25.2% (73/290) of the samples. Etiologic diagnosis included inflammation by viruses (bovine herpesvirus infection in six samples; ovine herpesvirus-2 infection in eight samples); and bacteria (bacterial infection in 34 samples; and tuberculos...

Arquivo Brasileiro De Medicina Veterinaria E Zootecnia, 2021

Dioctophymosis is the disease caused by Dioctophyma renale, a large nematode, popularly known as ... more Dioctophymosis is the disease caused by Dioctophyma renale, a large nematode, popularly known as giant kidney worm, and whose definitive hosts are the domestic dog and many wild mammal species. There are some reports of maned wolf parasitism by D. renale, however, in most cases the reports are restricted to the finding of the parasite during necropsy, without clinical assessment. The present report aimed to describe the clinical-therapeutic, ultrasonographic and pathological aspects of D. renale parasitism in a free-ranging maned wolf, emphasizing the treatment with an association of doramectin, praziquantel, pyrantel pamoate, and febantel that resulted in complete elimination of the parasite.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2020

A captive 7-year-old male bush dog (Speothos venaticus) was diagnosed with lymphoma affecting the... more A captive 7-year-old male bush dog (Speothos venaticus) was diagnosed with lymphoma affecting the kidneys, adrenal glands, liver, and spleen. The animal developed renal failure and was euthanized due to poor prognosis. Grossly, both kidneys were enlarged with multiple nodules. Histologically, the neoplasm was an infiltrative and poorly demarcated round cell tumor. Two morphologically distinct cell populations were observed, smaller cells with a lymphocytic morphology, and another population of larger and pleomorphic cells. Most of the smaller cell population, approximately 40% of the population within the neoplasm, were CD3 positive. Neoplastic cells were CD45, CD11d, and granzime B positive, and negative for CD20, CD79a, PAX5, CD163, and myeloperoxidase. This is the first reported case of lymphoma in a bush dog. This report demonstrated the suitability of several cell surface markers for differential diagnosis of round cell tumors in this species.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2018

A captive 12-year-old female maned wolf (Chrysocyon brachyurus), previously diagnosed with viscer... more A captive 12-year-old female maned wolf (Chrysocyon brachyurus), previously diagnosed with visceral leishmaniasis, developed severe myiasis in the right ear, followed by prostration and death. A firm solid nodule, measuring 1 x 3cm, was grossly observed in the pancreas. Histologically, there was neoplastic proliferation in the pancreas and pancreatic lymph node. Neoplastic cells had a neuroendocrine pattern, and were positive for gastrin and pancreatic polypeptide by immunohistochemistry. The wolf had also ulcerative gastritis. To the best of our knowledge is the first reported case gastrinoma in this species.

Brazilian Journal of Veterinary Pathology, 2018

Reported cases of neoplasia affecting hippopotamus are very scarce. This report describes a metas... more Reported cases of neoplasia affecting hippopotamus are very scarce. This report describes a metastatic oral squamous cell carcinoma in a captive 44-year-old male common hippopotamus (Hippopotamus amphibius) that developed progressive loss of appetite, fibrinohemorrhagic oral discharge, and progressive weight loss. Gross, cytological, and histopathological findings were compatible with a conclusive diagnosis of an invasive oral squamous cell carcinoma with metastasis in the lung, adrenal, and spleen. To the best of our knowledge this is the first report of squamous cell carcinoma in a common hippopotamus.

Brazilian Journal of Veterinary Pathology, 2018

Squamous cell carcinoma (SCC) is a common oral neoplasm in domestic cats, but there are only a fe... more Squamous cell carcinoma (SCC) is a common oral neoplasm in domestic cats, but there are only a few reports in wild felids. A captive 10-years-old, female Siberian tiger (Panthera tigris altaica), with history of chronic renal disease and serologically positive for Leishmania sp. developed a lytic mandibular nodule that was histologically diagnosed as SCC. At necropsy was also observed an exocrine pancreatic carcinoma, which was considered an incidental finding, and a chronic lympho-plasmocytic interstitial nephritis associated with a membranous glomerulopathy, compatible with the chronic renal disease presented by the animal. To the best of our knowledge this is the first report of an invasive and non-metastatic mandibular SCC in a Siberian tiger.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2017

Zebu and Holstein x Zebu crossbred have low incidence of uterine infection when compared to Holst... more Zebu and Holstein x Zebu crossbred have low incidence of uterine infection when compared to Holstein cows. Resistance to uterine infections may be associated with the ability to recognize invading microorganisms. Endometrial transcription of microbial molecular patterns receptors has been investigated in the postpartum period of Holstein cows, but it is completely unknown in Zebu or Holstein x Zebu cows. In this study, 9 Gyr and 12 F1 Holstein x Gyr cows were submitted to endometrial biopsies at the first and seventh days postpartum, with the objective to measure transcription levels of toll-like receptors (TLRs) 1/6, 2, 4, 5, and 9; nucleotide-binding oligomerization domain (NOD)-like receptors 1 and 2; and coreceptors cluster of differentiation 14 (CD14) and myeloid differentiation protein-2 (MD-2). There was a significant (P<0.05) decrease in transcription of TLR5 in Gyr, and an increase in transcription of TLR9 in F1 cows, between the first and seventh day postpartum. Both gr...

Brazilian Journal of Veterinary Research and Animal Science, 2017

Um crocodilo macho adulto (Crocodylus niloticus) foi diagnosticado com infecção fúngica sustêmica... more Um crocodilo macho adulto (Crocodylus niloticus) foi diagnosticado com infecção fúngica sustêmica. Histologicamente, havia extensas áreas de necrose no pulmão, que estavam associadas com infiltrado inflamatório linfo-plasmo-histiocitário, com numerosas células gigantes multinucleadas e miríade de pseudo-hifas e organismos leveduriformes intralesionais, dentro de favéolas distendidas. Focos necróticos também foram observados na mucosa do trato digestório, traquéia, túnica íntima de artérias, fígado e coração, com acentuado infiltrado inflamatório linfo-histiocitário, com grande número de macrófagos epitelioides e células gigantes e hifas e organismos leveduriformes intralesionais e intravasculares. Cortes corados por PAS e GMS evidenciaram estruturas leveduriformes ovais com 4 a 6 μm de diâmetro e pseudo-hifas de paredes espessas e paralelas com 5 a 8 μm. PCR realizado com DNA extraído de material parafinizado amplificou os domínios D1/D2 da subunidade maior do gene rRNA, cuja sequen...

Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2016

Journal of Comparative Pathology, 2016

Mycobacterial infections are rare in equines. Mycobacterium bovis (M. bovis) is an important zoon... more Mycobacterial infections are rare in equines. Mycobacterium bovis (M. bovis) is an important zoonotic bacterial pathogen causing disease in a wide range of animal species and sporadically causes severe disseminated disease in horses. This report describes the clinical, gross post-mortem examination and histopathological findings in a case of disseminated M. bovis infection in a donkey which to the authors' knowledge has not been previously documented in the scientific literature.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2015

Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2015

Dermatosparaxis is a genetic disease that affects collagen maturation. This disease is characteri... more Dermatosparaxis is a genetic disease that affects collagen maturation. This disease is characterized by marked impairment of the resistance of collagen fibers that leads to skin fragility, and it may affect several species. This is the first report of dermatosparaxis in sheep in Brazil. Clinically, two White Dorper lambs had multiple skin lacerations in the neck, dorsum and limbs. Transmission microscopy demonstrated irregular collagen fibers arranged in hieroglyphic shape and scanning electron microscopy demonstrated thin collagen fibrils that were not arranged in bundles as observed in the normal dermis. These findings are consistent with the diagnosis of dermatosparaxis.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2015

O Brasil possui o quarto maior rebanho equino do mundo, e o Estado de Minas Gerais detém o maior ... more O Brasil possui o quarto maior rebanho equino do mundo, e o Estado de Minas Gerais detém o maior número de equinos do país. Portanto, um diagnóstico preciso das doenças neurológicas dos equinos é prioridade no estado. Sendo assim, o objetivo deste estudo foi identificar, utilizando a Reação em Cadeia pela Polimerase (PCR), os agentes infecciosos responsáveis por enfermidades que afetam o sistema nervoso central (SNC) de equinos. De janeiro de 2009 a janeiro de 2011, foi realizado um levantamento dos casos de encefalites e encefalomielites em equinos no Estado de Minas Gerais, utilizando-se amostras de SNC de equinos que morreram com sinais neurológicos. Das 217 amostras de SNC, 47 (21,7%) foram positivas para o vírus da raiva pelo método de imunofluorescência indireta e inoculação em camundongos. Nas 170 amostras negativas para o vírus da raiva, o herpes-vírus equino-1 (EHV-1) foi diagnosticado em 20 (11,8%) e o herpes-vírus suíno-1 (SHV-1), em uma amostra por meio de PCR, e o vírus...

A case of chronic renal failure associated with septic polyarthritis affecting a 39-year-old male... more A case of chronic renal failure associated with septic polyarthritis affecting a 39-year-old male Western lowland gorilla (Gorilla gorilla gorilla) is described. The gorilla developed a chronic interstitial nephritis associated with severe diffuse renal fibrosis, which was associated with several extra-renal uremic lesions, including uremic pneumopathy and gastropathy. Several joints presented gross and microscopic changes compatible with chronic active arthritis and athrosis, which were associated with inflammation of adjacent soft tissues. Staphylococcus aureus was cultured from sites of phlegmon and cellulitis, whereas Enterobacter sp. and Proteus mirabilis were cultured from osteoarticular lesions. Additional conditions, including testicular atrophy and leydigocytoma, a large cell lung carcinoma, calcinosis circunscripta, among others, have also been diagnosed in this senile gorilla.

Arquivo Brasileiro De Medicina Veterinaria E Zootecnia, Jul 1, 2023

Chronic lead intoxication in a jaguar (Panthera onca) shot with round lead pelletscase report [In... more Chronic lead intoxication in a jaguar (Panthera onca) shot with round lead pelletscase report [Intoxicação crônica por chumbo em uma onça pintada (Panthera onca) alvejada com projéteis de chumborelato de caso]

Vaccines

Visceral leishmaniasis (VL) is a fatal disease caused by the protozoa Leishmania infantum for whi... more Visceral leishmaniasis (VL) is a fatal disease caused by the protozoa Leishmania infantum for which dogs are the main reservoirs. A vaccine against canine visceral leishmaniasis (CVL) could be an important tool in the control of human and CVL by reducing the infection pressure of L. infantum. Despite the CVL vaccine available on the market, the Brazilian Ministry of Health did not implement the use of it in their control programs. In this sense, there is an urgent need to develop more efficient vaccines. In this study, the association between two polymeric nanoformulations, (poly (D, L-lactic) acid (PLA) polymer) loading Leishmania amazonensis antigens, was evaluated as a potential immunobiological agent against VL using golden hamsters as an experimental model. The results indicated that no significant adverse reactions were observed in animals vaccinated with LAPSmP. LAPSmP presented similar levels of total anti-Leishmania IgG as compared to LAPSmG. The LAPSmP and LAPSmG groups sh...

Vaccines

Leishmaniasis is a widespread vector-borne disease in Brazil, with Leishmania (Leishmania) infant... more Leishmaniasis is a widespread vector-borne disease in Brazil, with Leishmania (Leishmania) infantum as the primary etiological agent of visceral leishmaniasis (VL). Dogs are considered the main reservoir of this parasite, whose treatment in Brazil is restricted to the use of veterinary medicines, which do not promote a parasitological cure. Therefore, efficient vaccine development is the best approach to Canine Visceral Leishmaniasis (CVL) control. With this in mind, this study used hamsters (Mesocricetus auratus) as an experimental model in an anti-Leishmania preclinical vaccine trial to evaluate the safety, antigenicity, humoral response, and effects on tissue parasite load. Two novel formulations of nanoparticles made from poly(D, L-lactic) acid (PLA) polymer loading Leishmania braziliensis crude antigen (LB) exhibiting two different particle sizes were utilized: LBPSmG (570 nm) and LBPSmP (388 nm). The results showed that the nanoparticles were safe and harmless to hamsters and ...

PLOS Neglected Tropical Diseases

Toxoplasmosis is an important zoonotic disease that affects a wide range of warm-blooded host spe... more Toxoplasmosis is an important zoonotic disease that affects a wide range of warm-blooded host species. Neotropical primates (New World Primates; NWP) are highly susceptible, developing a lethal acute systemic disease. Toxoplasmosis in free-ranging NWP is poorly described, with only a few studies based on serosurveys. Herein we performed a retrospective study focusing on the epidemiology and pathology of toxoplasmosis among 1,001 free-ranging marmoset (Callithrix spp.) deaths from the Brazilian Atlantic Forest. This study included marmosets necropsied at the Instituto Municipal de Medicina Veterinária Jorge Vaitsman (IJV) from January 2017 to July 2019, which were found dead from all regions in the State of Rio de Janeiro. Histopathology, immunohistochemistry, and transmission electron microscopy were performed to better characterize toxoplasmosis in this free-ranging population. All samples were also tested for Yellow Fever Virus (YFV) RT-qPCR by the official diagnostic service. A t...

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

Porcine respiratory disease complex is a major health concern for the porcine industry, causing s... more Porcine respiratory disease complex is a major health concern for the porcine industry, causing significant economic loss. In this study, a total of 156 samples from pigs referred to a diagnostic laboratory in Brazil for 15 months were analyzed by histopathology, bacterial isolation, PCR, and immunohistochemistry. Multiple infections were common, so 42.3% of the pigs had more than one pathogen detected in the lungs. Swine influenza virus was detected in 25.0% of the cases. Porcine circovirus type 2 was detected in 7.1% of the pigs, which was often associated with Pasteurella multocida. In addition, one case of porcine circovirus type 3 infection associated with granulomatous pneumonia was diagnosed. Bacteria were isolated in 125 cases, namely Pasteurella multocida (34.0%), Glaesserella (Haemophilus) parasuis (35.2%), Streptococcus suis (13.5%), and Actinobacillus pleuropneumoniae (7.7%). Mycoplasma hyopneumoniae was identified in 7.0% of the cases, and 18.6% of pigs carried Salmonel...

Pesquisa Veterinária Brasileira, 2017

ABSTRACT: This study evaluated the antimicrobial activity and acute or chronic toxicity of the ex... more ABSTRACT: This study evaluated the antimicrobial activity and acute or chronic toxicity of the extract of Stryphnodendron adstringens. The stem bark dry extract was obtained by static maceration with ethanol. Quantification of tannins was performed by the Folin-Denis method, which indicated a total tannin content of 32.7%. The antimicrobial activity of the dry extract of S. adstringens was evaluated by agar-based disk diffusion assay with Escherichia coli (ATCC 25922) and Staphylococcus aureus (ATCC 25923) in the concentration of 200, 400 and 600μL/mL. The results indicated that 600μL/mL inhibited microbial growth, i.e. had antimicrobial activity against these species. Acute and chronic toxic effects of S. adstringens was evaluated in Wistar rats treated with 200, 400, 600 and 800mg/kg of extract, administrated by gavage. Liver degeneration was observed in the group of rats receiving 800mg/kg in chronic exposure, what may indicate some degree of toxicity at this concentration. Howev...

Brazilian Journal of Veterinary Pathology, 2016

This study was based on analysis of the central nervous system from cattle that died after develo... more This study was based on analysis of the central nervous system from cattle that died after developing neurological disease in the State of Minas Gerais from 2004 to 2010. Samples were analyzed by histopathology, histochemistry, and anti- Listeria monocytogenes immunohistochemistry. The frequency of positivity for the techniques used and distribution were determined, and the results were analyzed by Fisher's exact test. Microscopic changes were observed in 29.5% (290/982) of bovine central nervous system samples examined. Inflammatory changes were the most frequent lesions (87.2% - 253/290) with 80% of cases (232/290) presenting non suppurative inflammation. Through histopathologic analyses, presumptive or conclusive etiology was obtained in 25.2% (73/290) of the samples. Etiologic diagnosis included inflammation by viruses (bovine herpesvirus infection in six samples; ovine herpesvirus-2 infection in eight samples); and bacteria (bacterial infection in 34 samples; and tuberculos...

Arquivo Brasileiro De Medicina Veterinaria E Zootecnia, 2021

Dioctophymosis is the disease caused by Dioctophyma renale, a large nematode, popularly known as ... more Dioctophymosis is the disease caused by Dioctophyma renale, a large nematode, popularly known as giant kidney worm, and whose definitive hosts are the domestic dog and many wild mammal species. There are some reports of maned wolf parasitism by D. renale, however, in most cases the reports are restricted to the finding of the parasite during necropsy, without clinical assessment. The present report aimed to describe the clinical-therapeutic, ultrasonographic and pathological aspects of D. renale parasitism in a free-ranging maned wolf, emphasizing the treatment with an association of doramectin, praziquantel, pyrantel pamoate, and febantel that resulted in complete elimination of the parasite.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2020

A captive 7-year-old male bush dog (Speothos venaticus) was diagnosed with lymphoma affecting the... more A captive 7-year-old male bush dog (Speothos venaticus) was diagnosed with lymphoma affecting the kidneys, adrenal glands, liver, and spleen. The animal developed renal failure and was euthanized due to poor prognosis. Grossly, both kidneys were enlarged with multiple nodules. Histologically, the neoplasm was an infiltrative and poorly demarcated round cell tumor. Two morphologically distinct cell populations were observed, smaller cells with a lymphocytic morphology, and another population of larger and pleomorphic cells. Most of the smaller cell population, approximately 40% of the population within the neoplasm, were CD3 positive. Neoplastic cells were CD45, CD11d, and granzime B positive, and negative for CD20, CD79a, PAX5, CD163, and myeloperoxidase. This is the first reported case of lymphoma in a bush dog. This report demonstrated the suitability of several cell surface markers for differential diagnosis of round cell tumors in this species.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2018

A captive 12-year-old female maned wolf (Chrysocyon brachyurus), previously diagnosed with viscer... more A captive 12-year-old female maned wolf (Chrysocyon brachyurus), previously diagnosed with visceral leishmaniasis, developed severe myiasis in the right ear, followed by prostration and death. A firm solid nodule, measuring 1 x 3cm, was grossly observed in the pancreas. Histologically, there was neoplastic proliferation in the pancreas and pancreatic lymph node. Neoplastic cells had a neuroendocrine pattern, and were positive for gastrin and pancreatic polypeptide by immunohistochemistry. The wolf had also ulcerative gastritis. To the best of our knowledge is the first reported case gastrinoma in this species.

Brazilian Journal of Veterinary Pathology, 2018

Reported cases of neoplasia affecting hippopotamus are very scarce. This report describes a metas... more Reported cases of neoplasia affecting hippopotamus are very scarce. This report describes a metastatic oral squamous cell carcinoma in a captive 44-year-old male common hippopotamus (Hippopotamus amphibius) that developed progressive loss of appetite, fibrinohemorrhagic oral discharge, and progressive weight loss. Gross, cytological, and histopathological findings were compatible with a conclusive diagnosis of an invasive oral squamous cell carcinoma with metastasis in the lung, adrenal, and spleen. To the best of our knowledge this is the first report of squamous cell carcinoma in a common hippopotamus.

Brazilian Journal of Veterinary Pathology, 2018

Squamous cell carcinoma (SCC) is a common oral neoplasm in domestic cats, but there are only a fe... more Squamous cell carcinoma (SCC) is a common oral neoplasm in domestic cats, but there are only a few reports in wild felids. A captive 10-years-old, female Siberian tiger (Panthera tigris altaica), with history of chronic renal disease and serologically positive for Leishmania sp. developed a lytic mandibular nodule that was histologically diagnosed as SCC. At necropsy was also observed an exocrine pancreatic carcinoma, which was considered an incidental finding, and a chronic lympho-plasmocytic interstitial nephritis associated with a membranous glomerulopathy, compatible with the chronic renal disease presented by the animal. To the best of our knowledge this is the first report of an invasive and non-metastatic mandibular SCC in a Siberian tiger.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2017

Zebu and Holstein x Zebu crossbred have low incidence of uterine infection when compared to Holst... more Zebu and Holstein x Zebu crossbred have low incidence of uterine infection when compared to Holstein cows. Resistance to uterine infections may be associated with the ability to recognize invading microorganisms. Endometrial transcription of microbial molecular patterns receptors has been investigated in the postpartum period of Holstein cows, but it is completely unknown in Zebu or Holstein x Zebu cows. In this study, 9 Gyr and 12 F1 Holstein x Gyr cows were submitted to endometrial biopsies at the first and seventh days postpartum, with the objective to measure transcription levels of toll-like receptors (TLRs) 1/6, 2, 4, 5, and 9; nucleotide-binding oligomerization domain (NOD)-like receptors 1 and 2; and coreceptors cluster of differentiation 14 (CD14) and myeloid differentiation protein-2 (MD-2). There was a significant (P<0.05) decrease in transcription of TLR5 in Gyr, and an increase in transcription of TLR9 in F1 cows, between the first and seventh day postpartum. Both gr...

Brazilian Journal of Veterinary Research and Animal Science, 2017

Um crocodilo macho adulto (Crocodylus niloticus) foi diagnosticado com infecção fúngica sustêmica... more Um crocodilo macho adulto (Crocodylus niloticus) foi diagnosticado com infecção fúngica sustêmica. Histologicamente, havia extensas áreas de necrose no pulmão, que estavam associadas com infiltrado inflamatório linfo-plasmo-histiocitário, com numerosas células gigantes multinucleadas e miríade de pseudo-hifas e organismos leveduriformes intralesionais, dentro de favéolas distendidas. Focos necróticos também foram observados na mucosa do trato digestório, traquéia, túnica íntima de artérias, fígado e coração, com acentuado infiltrado inflamatório linfo-histiocitário, com grande número de macrófagos epitelioides e células gigantes e hifas e organismos leveduriformes intralesionais e intravasculares. Cortes corados por PAS e GMS evidenciaram estruturas leveduriformes ovais com 4 a 6 μm de diâmetro e pseudo-hifas de paredes espessas e paralelas com 5 a 8 μm. PCR realizado com DNA extraído de material parafinizado amplificou os domínios D1/D2 da subunidade maior do gene rRNA, cuja sequen...

Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2016

Journal of Comparative Pathology, 2016

Mycobacterial infections are rare in equines. Mycobacterium bovis (M. bovis) is an important zoon... more Mycobacterial infections are rare in equines. Mycobacterium bovis (M. bovis) is an important zoonotic bacterial pathogen causing disease in a wide range of animal species and sporadically causes severe disseminated disease in horses. This report describes the clinical, gross post-mortem examination and histopathological findings in a case of disseminated M. bovis infection in a donkey which to the authors' knowledge has not been previously documented in the scientific literature.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2015

Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2015

Dermatosparaxis is a genetic disease that affects collagen maturation. This disease is characteri... more Dermatosparaxis is a genetic disease that affects collagen maturation. This disease is characterized by marked impairment of the resistance of collagen fibers that leads to skin fragility, and it may affect several species. This is the first report of dermatosparaxis in sheep in Brazil. Clinically, two White Dorper lambs had multiple skin lacerations in the neck, dorsum and limbs. Transmission microscopy demonstrated irregular collagen fibers arranged in hieroglyphic shape and scanning electron microscopy demonstrated thin collagen fibrils that were not arranged in bundles as observed in the normal dermis. These findings are consistent with the diagnosis of dermatosparaxis.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2015

O Brasil possui o quarto maior rebanho equino do mundo, e o Estado de Minas Gerais detém o maior ... more O Brasil possui o quarto maior rebanho equino do mundo, e o Estado de Minas Gerais detém o maior número de equinos do país. Portanto, um diagnóstico preciso das doenças neurológicas dos equinos é prioridade no estado. Sendo assim, o objetivo deste estudo foi identificar, utilizando a Reação em Cadeia pela Polimerase (PCR), os agentes infecciosos responsáveis por enfermidades que afetam o sistema nervoso central (SNC) de equinos. De janeiro de 2009 a janeiro de 2011, foi realizado um levantamento dos casos de encefalites e encefalomielites em equinos no Estado de Minas Gerais, utilizando-se amostras de SNC de equinos que morreram com sinais neurológicos. Das 217 amostras de SNC, 47 (21,7%) foram positivas para o vírus da raiva pelo método de imunofluorescência indireta e inoculação em camundongos. Nas 170 amostras negativas para o vírus da raiva, o herpes-vírus equino-1 (EHV-1) foi diagnosticado em 20 (11,8%) e o herpes-vírus suíno-1 (SHV-1), em uma amostra por meio de PCR, e o vírus...

A case of chronic renal failure associated with septic polyarthritis affecting a 39-year-old male... more A case of chronic renal failure associated with septic polyarthritis affecting a 39-year-old male Western lowland gorilla (Gorilla gorilla gorilla) is described. The gorilla developed a chronic interstitial nephritis associated with severe diffuse renal fibrosis, which was associated with several extra-renal uremic lesions, including uremic pneumopathy and gastropathy. Several joints presented gross and microscopic changes compatible with chronic active arthritis and athrosis, which were associated with inflammation of adjacent soft tissues. Staphylococcus aureus was cultured from sites of phlegmon and cellulitis, whereas Enterobacter sp. and Proteus mirabilis were cultured from osteoarticular lesions. Additional conditions, including testicular atrophy and leydigocytoma, a large cell lung carcinoma, calcinosis circunscripta, among others, have also been diagnosed in this senile gorilla.