Trina Tallei | Universitas Sam Ratulangi (original) (raw)

Conference Presentations by Trina Tallei

A compilation of abstracts and articles presented in Seminar & Workshop: women and Health: Towar... more A compilation of abstracts and articles presented in Seminar & Workshop: women and Health: Towards a better understanding and environment in the future. The event organized by the Faculty of Medicine - UKI< Jakarta, German Academic Exchange Service (DAAD), German-Indonesian Medical Association (DIGM) and Indostaff on 21-24 November 2011.

Papers by Trina Tallei

This paper discusses the current issues on achieving sustainable food security through understand... more This paper discusses the current issues on achieving sustainable food security through understanding the role of indigenous knowledge in conserving plant and genetic diversity of local agricultural product in Minangkabau. Minangkabau is an ethnic group indigenous to the highlands of West Sumatera (Indonesia). It is amongst the world’s largest matrilineal society. In Minangkabau ceremonies, food plays a central role which honour religious and life cycle rites. The food ingredients and spices are rich in biodiversity, creating a demand for genetic diversity conservation for supporting food security in the future. Conservation of genetic diversity of the ingredients and spices are passed through indigenous knowledge as verbal communications which consist of beliefs, customs, tools, techniques, and rituals. These kinds of knowledge are in sense of producing, choosing, and preparing foods’ ingredients and spices. The Minangkabau maintains their farmland in a unique way in order to achiev...

HAYATI Journal of Biosciences, 2016

Areca vestiaria Giseke (Pinang Yaki) is an original palm plant of Sulawesi which spread over Lore... more Areca vestiaria Giseke (Pinang Yaki) is an original palm plant of Sulawesi which spread over Lore Lindu National Park, Bogani Nani Wartabone National Park, Ambang Mount Nature Reserve, slope of Soputan and of Mahawu Mountains. This plant can also be found in Maluku, spread over Halmahera and Seram islands and recognized there as Pinang Merah. The plant has been used by Bolaang Mongondow tribe in North Sulawesi for treating diabetic and means of contraception. Principally this research aimed at exploring the potential of Pinang Yaki as a medicinal plant. Result of proximate analysis indicated that extract of Pinang Yaki contained water (5.78%,) organic solvent (16.46%), and ash (0,70%). Analysis of phytochemistry showed that its extract contained tannin, flavonoid, hydroquinone, triterpenoid, and saponin.

Damselfly (Zygoptera) plays important roles in the environment and this organism ia able to be us... more Damselfly (Zygoptera) plays important roles in the environment and this organism ia able to be used as biocontrol and bioindicator. This research was aimed to analyze the abundance of damselfly that werelocated at three different habitats in Bogani Nani Wartabone National Park, North Sulawesi. The purposive random sampling method was used to collect the samples. Damselflies were taken by using sweeping technique following 500 m transect line in each habitat. There were three transect lines in each habitat located along the river. The results showed that there were thirteen species from 4 families (Coenagrionidae, Chlorocypidae, Calopterygidae, and Platycnemididae) of damselfly. Most species were members of Coenagrionidae whereas the others werePlatycnemididae Family. Based on the types of habitat, the highest number of species was found in the primary forest, whereas the smallest number was in the secondary forest. Greatest the abundance of damselfly was the largest in the agricultu...

Forest stratification refers to the different layers of plants in a forest. In a mature forest, t... more Forest stratification refers to the different layers of plants in a forest. In a mature forest, there are typically several distinct layers of vegetation rising from the forest floor to the tree canopy. This research, which conducted at Tangkoko-Batuangus Nature Reserve from October 2006 to January 2007, focused on utilization of five stratification of tropical rain forest by crested black Macaques (Macaca nigra) during the daily activities of the species. Data were collected from two habituated groups called Rambo Group I and Rambo Group II. Total samples were 21 individuals of Rambo Group I and 17 individuals of Rambo Group II. Daily activities ranged from feeding, foraging, moving, resting, and social interaction that collected by focal animal sampling with 2-minute intervals. Forest was divided vertically into 5 layers namely Layer-A (40-50 m), Layer-B (18-39 m), Layer-C (4-17), Layer-D (1-3 m), and Layer E (0-1 m). Total observation was 12,829 in Rambo Group I and 12,609 in Ram...

This paper discusses about the action of Sam Ratulangi University in term of implementing worksho... more This paper discusses about the action of Sam Ratulangi University in term of implementing workshop and field training about Sulawesi Biodiversity and It’s Conservation. The objective of this program was to train high school Biology teachers about Sulawesi Biodiversity and Conservation and followed by construction of biology curriculum based on Sulawesi Biodiversity and Conservation for North Sulawesi High schools. The topics for this workshop included : Sulawesi biodiversity, threats to biodiversity, conservation of biodiversity, biodiversity and conservation under Indonesian law, evaluation of Biology curriculum in high schools, and construction of Biology curriculum based on biodiversity and conservation. The materials of the training were Conservation Areas, Management of Conservation Areas, Recognition on various kinds of Ecosystem, Identification and Population Survey on Birds and Animals, and Individual Task. To evaluate the success of this training, pretest and post-test were...

Sulawesi crested black macaques (Macaca nigra) is one of monkey species that is endemic to the No... more Sulawesi crested black macaques (Macaca nigra) is one of monkey species that is endemic to the Northern Peninsula of Sulawesi Island. They live in a social group in tropical rain forest, either in primary or in secondary forest. A highest safety site of their range is Tangkoko-Batuangus Nature Reserve in North Sulawesi Province. In 1994 a group, called Rambo, was split into two smaller groups, namely Rambo I and Rambo II. Up to 1994, the group size of Rambo was 97 individuals. After being split, each of the Rambo groups has a stable population within the group which consisted of 50-60 individuals. Rambo had 406 ha of home range and 3.1 ± 0.5 km of day range. On the other hand, Rambo I has 315 ha of home range and 2.8 ± 0,7 km of day range, and Rambo II has 395 ha of home range and 3.7 ± 0,9 km of day range. Comparing their daily activities, Rambo had to move shorter distance than their offspring groups. This can be explained by habitat destruction perspective so that they had to div...

Climate Change Management, 2011

Bitung is a city located in North Sulawesi province in Indonesia. It is a unique city due to havi... more Bitung is a city located in North Sulawesi province in Indonesia. It is a unique city due to having three conserved forests. Covering an area of 304 km 2 with a population of about 175,000 inhabitants, the need for clean water for Bitung is high. In the midst of the difficulties of having clean water available, there are people who have an important role in maintaining clean water resources for the community needs in the city. These are people from Danowudu sub-district who still retain 21.5 ha of forest to maintain the availability of clean water for Bitung. Local wisdom is the community's local knowledge used by the community in carrying out environmental conservation for the sake of harmonious interaction between man and environment. To retain the sustainability of the forest and a clean water source, the indigenous community implements customary rules that entail customary sanctions to offenders. These include prohibition of the destruction and taking of forest products, as well as the obligation to maintain the forest's sustainability. Local wisdom of Danowudu community has been proved to be very effective in preserving the city forests so that the source of clean water for the city of Bitung is well maintained.

This study describes a study on waste management in Indonesia. Wastes produced by the inhabitants... more This study describes a study on waste management in Indonesia. Wastes produced by the inhabitants of Bunaken Island and transported by sea from Manado city, contribute to the destruction of the marine ecosystem in Bunaken National Marine Park. Urgent and smart steps need to be taken in order to manage the waste on the island and its sea shore. This study elaborates on a multiparty collaboration program in environmentally sound waste management as an effort to mitigate the impact of waste on the Bunaken sea garden ecosystem. The program covers waste management, community empowerment and a common vision to reduce waste from its source. Its flagship program is the community-based “Rubbish Bank”. The program included socialization of the Rubbish Bank, setting up of a waste management hierarchy and feedback from the community. Due to its approach and scope, this study provides a best-practice example of local community-based initiatives to manage waste in a developing country context, by...

Tarsius spectrum is an endemic primate of Sulawesi. This primate is categorized into lower risk a... more Tarsius spectrum is an endemic primate of Sulawesi. This primate is categorized into lower risk and recommended as vulnerable and protected species. They have a small round body with nocturnal activity. The research was conducted on primary forest of Tangkoko-Batuangus Nature Reserve (TBNR) in North Sulawesi, 1°30'-1°34'N and 125°10'-125°81'E, in May 2011-August 2012. This research was aimed to know tarsiers' population density by census and estimation methods. A 121 of 100 m 2 -sized plot was used for population census. Distance of plots was 100 m each and 16-circle plots (r = 100 m) were used for population estimation. Distance between central point plots was 200 m. There were 57 duet call locations inhabited by 229 animals. The population density was 47 groups/km 2 , consisted of 209 adults, 11 sub-adults and 9 infants. Population density was 189 animals/km 2 . The individual in each group was 4.01±1.83 with modus 4. This research showed that population densit...

We characterized the molecular structure of chitin and chitosan isolated from the biomass of cult... more We characterized the molecular structure of chitin and chitosan isolated from the biomass of cultivated rotifer (B. rotundiformis). Chitin and chitosan are potential biomaterials for biotechnological industries due to their structural and functional characteristics. Zooplankton seems to be a better source of chitin, associated with the very low degree of calcification and sclerotization of the cuticles. Cultivation of local strain rotifer from North Sulawesi waters has been successfully conducted in a very simple medium with raw fish input without aeration in fiber tanks. This mass production technology has enabled to harvest 45-48 million individuals with estimated 5 to 48 g rotifer biomass in each production cycle. From this rotifer biomass, 4.6% chitin was extracted and 52.7% chitosan could be derived by deacetylation of chitin. Structural characteristics of the rotifer chitin and chitosan were discussed on the basis of the X-ray and infrared analysis data. X-ray analysis was done using X-Ray Defraction (XRD) with CuK radiation at a voltage of 40 kV and 30 m A. X-ray diffraction diagram of chitin indicated the molecular form at three strongest peaks, 8.1, 9.2 and 19 2θ, differed from that of chitosan at peaks, 9.6, 19.5 and 21.1. Christalinity of chitosan (47.06%), was higher than that of chitin (33.94%). Molecular structure of chitin, C 18 H 26 N 2 O 10 , where the hydroxyl group on the second carbon replaced by acetyl amide, was shown by the infrared spectra. In the infrared spectra, the rotifer chitin indicated the amide II band at 1558.48 cm -1 , a typical one for marine chitin. A marked difference was observed for the amide I bands, at 1651.07 cm -1 . Chitosan showed no amide band, but hydroxyl and amino bands at the ranged spectra up to 3500 cm -1 . The yields of chitin isolated from rotifer biomass and its deacetylated products (chitosan) were relatively small and as a polymer of N-acetyl D-glucosamine, both molecules showed difference in specific functional groups. Chitin is composed of 2-acetamido-2-deoxy-D-glucose, while chitosan composed primarily of glucosamine, 2-amino-2-deoxy-D-glucose. Some treatments are necessary to confirm the molecular conformation and deacetylation behavior. Chitosan could be more accessible for structural modifications to develop biocompatible materials for pharmaceutical purposes.

Chitin and chitosan from rotifer have not been previously explored due to the problem of high bio... more Chitin and chitosan from rotifer have not been previously explored due to the problem of high biomass required for extraction. This study aimed to obtain the chitin yield from rotifer biomass produced in mass culture, and to characterize the basic properties of chitin and chitosan, especially, nitrogen content and dye binding capacity. Methods of extraction and deacetylation of chitin were adopted from Chandumpai et al. (2004) with modification. The nitrogen content was analyzed using the semi-micro Kjeldahl method (AOAC, 1984), and dye binding capacity using the method developed by Cho et al. (1998). Results show that the yield of chitin obtained from the rotifer sample was relatively small (4.64%), and the yield of chitosan was even smaller, only 2,62%. The proportion of chitosan over chitin was 52,4%. The nitrogen content of chitin and chitosan of rotifer were 4.23 to 4.36% and 7.12-7.23%, respectively. The capacity of chitin to maintain the bonded dye was relatively stronger tha...

Nutmeg (family: Myristicaceae) is a plant that originated from Banda islands and is widely cultiv... more Nutmeg (family: Myristicaceae) is a plant that originated from Banda islands and is widely cultivated in several places in the world. Secondary metabolites of this plant have a high value because of their benefits for the health, food, and beauty industries. This study aims at developing DNA barcode for nutmeg (Myristica fragrans) using standard recommended fragment of matK (maturase K) gene. Universal matK primer pairs were used to amplify 889 bp DNA fragment. BLAST search from NCBI site showed that Sangihe nutmeg has 100% identity with Myristica fatua, M. maingayi, and M. globosa. It also has 3 nucleotides difference with Rivola sebifera (identity 99.58%) and 4 nucleotides difference with Knema laurina (identity 99.43%). It can be inferred from this study that single locus of matK gene cannot be used to differentiate species in Myristica; it can only be used to differentiate the genus level within family Myristicaceae.

The UNISTAFF program from ISOS, University Kassel Germany has brought together sixty scholars fro... more The UNISTAFF program from ISOS, University Kassel Germany has brought together sixty scholars from various universities in Indonesia. These scholars come from different scientific backgrounds. Upon their return they are expected to become agents-of-change and create a multiplier effect to contribute improvement of quality in higher education in their respective countries. One example of this multiplier effect has arisen from the implementation of an alumni project in Sam Ratulangi University, Manado, Indonesia. During the course of preparations for the project, contact was established with a professor of Genetics in University Kassel, Germany. The initial involvement of the German professor was as a resource person for a Molecular Biology seminar cum workshop developed at Sam Ratulangi University, Manado. The active involvement of the INDOSTAFF (Indonesian Unistaff Alumni) Country Coordinator has led to further developments in terms of developing a network in Biodiversity Teaching, ...

HAYATI Journal of Biosciences, 2016

Climate Change Management, 2011

Petroleum contamination and its derivate in ecosystem are considered as environmental threat all ... more Petroleum contamination and its derivate in ecosystem are considered as environmental threat all over the world. Some microorganisms exhibit potential to degrade hydrocarbon in contaminated environments. This study aims at identifying potential diesel oil-degrading bacteria grown on artificial media. Bacteria isolated from Manado Sea port were grown in nutrient agar containing artificial diesel oil plus salt water and diesel oil only, respectively. The growing bacteria were isolated and each of them was grown separately to obtain pure isolate. Three bacterial isolates namely AO2, OA3 and OA4 were identified using 16S rRNA gene as Pseudomonas aeroginosa, Klebsiella oxytoca, and Citrobacter sp, respectively. Keywords: diesel oil, diesel oil-degrading bacteria, Manado Sea Port, 16S rRNA gene IDENTIFIKASI BAKTERI YANG BERPOTENSI SEBAGAI PENDEGRADASI MINYAK DIESEL DI ISOLASI DARI PELABUHAN LAUT MANADO ABSTRAK Kontaminasi minyak bumi dan turunannya dalam ekosistem dianggap sebagai ancaman...