MARIO E ARENA | Universidad Nacional de Tucuman (original) (raw)

Papers by MARIO E ARENA

Annals of Microbiology, 2007

Abstract This work was carried out to determine the activity of enzymes involved in arginine meta... more Abstract This work was carried out to determine the activity of enzymes involved in arginine metabolism inLactobacillus plantarum isolated from wine and previously characterised at molecular level. The activity of the enzymes arginine deiminase and ornithine ...

Inorganica Chimica Acta, 2015

a b s t r a c t The reaction in alkaline aqueous solution between sulfamethoxazole (SMX) and mang... more a b s t r a c t The reaction in alkaline aqueous solution between sulfamethoxazole (SMX) and manganese (II) chloride produces colorless crystals with formula [Mn(H 2 O) 6 ] 0.5 [Mn(SMX) 3 ], which was characterized by UV-Vis, IR and Raman spectroscopy and thermal analysis. The crystal structure of [Mn(H 2 O) 6 ] 0.5 [Mn(SMX) 3 ] complex has been solved by X-ray diffraction methods. It crystallizes in the cubic Pa-3 space group with a = 19.5476(1) Å, and Z = 8 molecules per unit cell. [Mn(SMX) 3 ] À complex is at a crystallographic special position of C 3 symmetry with the Mn(II) ion cis-coordinated to three equivalent sulfamethoxazole molecules acting as bidentate ligands in a propeller-like conformation. [Mn(H 2 O) 6 ] 2+ hydrate ion is at crystal special position of S 6 symmetry with the metal in an almost perfect octahedral coordination with six water molecules. At 100 lg/mL Mn(H 2 O) 6 ] 0.5 [Mn(SMX) 3 ] and SMX, inhibited the Staphylococcus aureus biofilm formation by 92% and 54%, respectively. However, at the same concentration Mn(H 2 O) 6 ] 0.5 [Mn(SMX) 3 ] and SMX inhibited the planktonic bacterial growth by 52% and 81%, respectively. The Mn(II) complex inhibited the biofilm formation in values higher than 35% at the concentration 0.5 lg/mL. These results suggest that the metal complex [Mn(H 2 O) 6 ] 0.5 [Mn(SMX) 3 ] is a good candidate for the development of new antimicrobial agent acting in part as bactericidal but mainly as antipathogenic agent.

Journal of Natural Products, Nov 1, 2010

Seven new prenylated acylphloroglucinols, yungensins A-G, were isolated from the diethyl ether ex... more Seven new prenylated acylphloroglucinols, yungensins A-G, were isolated from the diethyl ether extract of the scaly rhizomes and roots of an Argentine collection of the fern Elaphoglossum yungense. All the compounds contained a geranyl group attached to the filicinic acid-type residue. The diethyl ether extract and yungensins A-F displayed antibacterial effects against Staphylococcus aureus and Pseudomonas aeruginosa with MICs ranging from 10 to 200 μg/mL. All tested acylphloroglucinols, except for yungensin D, caused bacteriolysis of S. aureus at MBC or higher doses (50-200 μg/mL). At 10 μg/mL, the ether extract as well as yungensins A, B, and D-F altered the biofilm production in both microoganisms.

Vii Jornadas Cientificas De Los Grupos De Investigacion Enologica Gienol Logrono 21 22 Y 23 De Mayo De 2003 2003 Isbn 84 8125 201 8 Pags 276 278, 2003

Sugarcane is rich in dietary fibers that have beneficial properties in gastrointestinal tract. In... more Sugarcane is rich in dietary fibers that have beneficial properties in gastrointestinal tract. In addition the sugarcane is well known for its protective role in mammalian health. However, the sugarcane industry produces vast quantities of residues. Sugarcane blunting (waste produced by the sugarcane industry) was fermented under different conditions: using native microbiota (control), using specific goat probiotics, including Lactobacillus reuteri DDL19 (Treatment Nº1), Lactobacillus alimentarius DDL 48 (Treatment Nº2), Enterococcus faecium DDE 39 (Treatment Nº3), Bifidobacterium bifidum DDBA (Treatment Nº4) and a probiotic mixture containing equal amounts of the four probiotics (Treatment N°5). The fermented sugarcane is a synbiotic supplement for goats because it contains goat probiotic as well as dietary fibers considered as prebiotic. Firstly, the ensilages were microbiologically examined and then administered simultaneously to different batches of six goats each immediately af...

Global Journal of Agricultural Innovation, Research & Development, 2014

Polygonum punctatum Elliot (water pepper) is a pungent herb ancestrally employed as a disinfectan... more Polygonum punctatum Elliot (water pepper) is a pungent herb ancestrally employed as a disinfectant in traditional medicine by Toba Indians of the north-eastern region of Argentina and also commonly used as spice in Japanese cuisine. GC-MS of whole diethyl ether extract (EE) from aerial parts allowed to identify 14 volatile terpenoids such as sesquiterpenes: α-bisabolol (3.4 %), polygodial and isotadeonal (34.0%); various methylated phenol like αtocopherol or vitamin E (3.6 %), and phytosterols: stigmasterol (2.1%) and β-sitosterol (29.9 %). Thus, water pepper is a promising source of drimane sesquiterpenes and phytoestrogens with important bioactivities.

Phytomedicine : international journal of phytotherapy and phytopharmacology, Jan 15, 2015

Five dammarane-type triterpenoids, five pentacyclic triterpenoids (three of them carrying a carbo... more Five dammarane-type triterpenoids, five pentacyclic triterpenoids (three of them carrying a carboxylic acid group), and two aromadendrane-type sesquiterpenoids were isolated from an Argentinian collection of the liverwort Lepidozia chordulifera. Compounds were characterized by comparison of their spectral data with those previously reported and tested in their ability to control bacterial growth, biofilm formation, bacterial Quorum Sensing process (QS), and elastase activity of Pseudomonas aeruginosa, as well as bacterial growth and biofilm formation of Staphylococcus aureus. The key role played by biofilm and elastase activity in bacterial virulence make them a potential target for the development of antibacterial agents. The aromadendrane-type sesquiterpenoid viridiflorol was the most potent biofilm formation inhibitor, producing 60% inhibition in P. aeruginosa and 40% in S. aureus at 50µg/ml. Ursolic and betulinic acids (two of the pentacyclic triterpenoids isolated) were able to...

Public Health Microbiology, 2004

Biogenic amines can be formed and degraded as a result of normal metabolic activity in animals, p... more Biogenic amines can be formed and degraded as a result of normal metabolic activity in animals, plants, and microorganisms and are usually produced by the decarboxylation of amino acids. Recent trends in food security are promoting an increasing search for trace compounds that can affect human health. Although they are present in fermented foods and beverages in low quantities, they exhibit interactions with normal human metabolism (e.g., having vasoactive or psychoactive properties) that justify the research on their presence in foods and the possible related toxicological effects that they may cause. Estimation of the biogenic amines histamine, tyramine, agmatine, putrescine, and cadaverine is important not only from the point of view of their toxicity, but also because they can be used as indicators of the degree of freshness or spoilage of food. Until recently, because of the difficulty in detecting and quantifying amines reliably we have had insufficient information about their occurrence in different types of foods and beverages. These problems are related to matrix interference (e.g., the presence of free amino acids) and the low levels at which the amines are found. Early techniques for the determination of biogenic amines in foods were based on thin-layer chromatography. More modern analytical techniques have since been developed that allow the acquisition of reliable quantitative data and better separation/resolution of various amines. The quantitative determination of biogenic amines is generally accomplished by overpressure-layer chromatography, high-performance liquid chromatography (HPLC), and gas chromatography. The use of reverse-phase column and precolumn derivatization was more efficient and faster than the conventional ion-exchange techniques. This study was conducted to evaluate two HPLC derivatization methods for quantitative determination of biogenic amines: the method described by Gonzales de Llano et al. for amino acid analysis and the method described by Eerola et al.

Food and Nutrition Sciences, 2013

Different peppers fermentations (Capsicum annum, grossum variety) were assayed: spontaneous, nati... more Different peppers fermentations (Capsicum annum, grossum variety) were assayed: spontaneous, native microflora supplemented individually with Lactobacillus plantarum N8, Leuconostoc mesentereroides L. or Pediococcus pentosaceus 12p and by pure or combined cultures of these lactic acid bacteria (LAB). In order to eliminate the native flora, different kinds of heat treatment were assayed. The treatment selected was heating in autoclaved after research 3/4 atmosphere and to turn off. Fermentations were carried out at 22˚C and 30˚C and the culture media contained 2% or 0.2% glucose and 4% NaCl. Sugar consumption, pH reduction and acid production were higher at 30˚C than at 22˚C. At both temperatures, spontaneous fermentation showed a slower rate reduction in pH than inoculated samples. Diminution in pH in presence of 2% glucose was faster than at 0.2%, but minimum pH was in both case lower than 3.0. Maximum growth was reached between 2 and 5 days of fermentation in all the samples assayed. After 30 days of incubation in presence of 2% glucose the survival of LAB was nearly 5 log ufc/ml. The survival was higher at the lower temperature assayed for both glucose concentrations. Organoleptic properties of peppers fermented with a mixed culture of Leuconostoc mesenteroides and Pediococcus pentosaceus were found best by a human panel. This sample has a relation lactic acid/acetic acid of nearly 3 in the conditions assayed.

Journal of Dairy Science, 2015

The effect of a mixture of potentially probiotic bacteria (MPPB; Lactobacillus reuteri DDL 19, La... more The effect of a mixture of potentially probiotic bacteria (MPPB; Lactobacillus reuteri DDL 19, Lactobacillus alimentarius DDL 48, Enterococcus faecium DDE 39, and Bifidobacterium bifidum strains) on the milk fatty acid (FA) profile, with emphasis on cis-9,trans-11 conjugated linoleic acid (CLA) in the middle stage of goat lactation, was determined. In addition, the effects of MPPB feeding on the FA profile in intestinal content and intestinal morphology in weaned goats were analyzed. The probiotic supplement was able to modify FA composition of milk and intestinal content. The unsaturated FA concentrations in milk (g of FA/L of milk) increased from 4.49 to 7.86 for oleic (18:1), from 0.70 to 1.39 for linoleic (18:2), from 0.063 to 0.187 for linolenic (18:3) acid, and from 0.093 to 0.232 for CLA. The atherogenicity index diminished 2-fold after MPPB ingestion. In the intestinal content of the weaned goats, no significant difference in saturated FA concentration compared with the control was observed. However, oleic acid, linolenic acid, CLA, and docosahexaenoic acid concentrations increased by 81, 23, 344, and 74%, respectively, after probiotic consumption. The ruminal production of CLA was increased by the MPPB. However, bacterial strains of MPPB were unable to produce CLA in culture media. By histological techniques, it was observed that the treated group had intestinally more conserved morphological structures than the control group. The results obtained in this study indicate that the MPPB administration in lactating and weaned goats allows for the production of milk with improved concentrations of beneficial compounds, and also produces a protective effect in the goat intestine. The results obtained in this study reinforce the strategy of probiotics application to enhance goat health with the production of milk with higher concentrations of polyunsaturated FA.

Handbook of Plant-Based Fermented Food and Beverage Technology, Second Edition, 2012

Anaerobe, 2014

The mutagen binding ability of the goat probiotics (Lactobacillus reuteri DDL 19, Lactobacillus a... more The mutagen binding ability of the goat probiotics (Lactobacillus reuteri DDL 19, Lactobacillus alimentarius DDL 48, Enterococcus faecium DDE 39, and Bifidobacterium bifidum DDBA) was evaluated. The oral administration of these probiotics reduced fecal mutagens and intestinal cancer markers in goats. Secondly, the effects of probiotics against the mutagenesis induced by sodium azide (SA), and Benzopyrene (B[α]P) by performing the modified Ames test using Salmonella typhimurium TA 100 was investigated. The capacity to bind benzopyrene and the stability of the bacterial-mutagen complex was analyzed by HPLC. The dismutagenic potential against both mutagens was proportional to probiotic concentration. Results showed that probiotic antimutagenic capacity against SA was ranging from 13 to 78%. The mixture of four goat probiotics (MGP) displayed higher antimutagenic activity against SA than any individual strains at the same cell concentration. This study shows that the highest diminution of mutagenicity in presence of B[α]P (74%) was observed in presence of MGP. The antimutagenic activity of nearly all the individual probiotic and the MGP were in concordance with the B[α]P binding determined by HPLC. According to our results, the B[α]P binding to probiotic was irreversible still after being washed with DMSO solution. The stability of the toxic compounds-bacterial cell binding is a key consideration when probiotic antimutagenic property is evaluated. MGP exhibits the ability to bind and detoxify potent mutagens, and this property can be useful in supplemented foods for goats since it can lead to the removal of potent mutagens and protect and enhance ruminal health and hence food safety of consumers.

Pharmaceutical Biology, 2015

Context: It is necessary to advance the field of alternative treatments for chronic wounds that a... more Context: It is necessary to advance the field of alternative treatments for chronic wounds that are financially accessible to the least economically developed countries. Previously we demonstrated that topical applications of Lactobacillus plantarum culture supernatants (LAPS) on human-infected chronic wounds reduce the pathogenic bioburden, the amount of necrotic tissue, and the wound area, as well as promote debridement, granulation tissue, and wound healing. Objective: To study LAPS chemically and biologically and to find potential molecules responsible for its pro-healing and anti-pathogenic properties in chronic wounds. Materials and methods: (1) Chemical analysis: extracts were subjected to a column chromatography and the fractions obtained were studied by GCMS. (2) Quantification: DL-lactic acid (commercial kit), phenolic compounds (Folin-Ciocalteu), H 2 O 2 (micro-titration), and cations (flame photometry). (3) Biological analysis: autoinducers type 2 (AI-2) (Vibrio harveyi BB170 bioassay), DNAase activity (Agar DNAase), and Pseudomonas aeruginosa biofilm inhibition (crystal violet technique). Results: According to its biological activity, the most significant molecules found by GCMS were the following: antimicrobials (mevalonolactone, 5-methyl-hydantoine, benzoic acid, etc.); surfactants (di-palmitin, distearin, and 1,5-monolinolein); anesthetics (barbituric acid derivatives), and AI-2 precursors (4,5-dihydroxy-2,3-pentanedione and 2-methyl-2,3,3,4tetrahydroxytetrahydrofurane). Concentrations measured (mg/mL): DL-lactic acid (11.71 ± 1.53) and H 2 O 2 (36 ± 2.0); phenolic compounds (485.2 ± 15.20); sodium (370 ± 17); potassium 920 ± 24); calcium (20 ± 4); and magnesium (15 ± 3). DNAase from LAPS had activity on genomic DNA from PMNs and P. aeruginosa. Discussion and conclusion: The molecules and biological activities found in LAPS could explain the observed effects in human chronic wounds.

Phytotherapy research : PTR, 2011

Acanthospermal B (AcB), the major sesquiterpene lactone (SL) of Acanthospermum hispidum, an herb ... more Acanthospermal B (AcB), the major sesquiterpene lactone (SL) of Acanthospermum hispidum, an herb widely spread in Argentina, is a selective antibacterial agent against Enterococcus faecalis and Staphylococcus aureus, but inactive on Gram-negative and Lactobacillus. Methicillin-resistant Staphylococcus aureus (MRSA) is one of the main microorganisms involved in human chronic infection. A balb\c mouse skin infection model was developed to reproduce the lesions caused by acute and chronic infections produced by MRSA. After determination of the maximum concentration of AcB unable to produce tissular injury after intradermal injection, the anti-MRSA effect of AcB was evaluated on skin, liver and spleen tissues of infected mice. AcB, at doses of 2.5 mg/kg, produced a ten times decrease of MRSA growth in skin infection. In addition, the same dose prevented the dissemination to liver and/or spleen. AcB also displayed a bacteriostatic effect, in vitro, on MRSA cultures at 50 µg/mL that seems...

Journal of the Science of Food and Agriculture, 2013

BACKGROUND: Lactobacillus hilgardii, a wine lactic acid bacterium, is able to use arginine, throu... more BACKGROUND: Lactobacillus hilgardii, a wine lactic acid bacterium, is able to use arginine, through the arginine deiminase pathway with the formation of citrulline, a precursor of the carcinogen ethyl carbamate. The influence of different Argentine wine varieties (Merlot, Cabernet Sauvignon and Malbec), on bacterial growth and arginine metabolism was examined. Furthermore, the effect of different components normally present in wines on the enzyme activities of the arginine deiminase system was determined.

Journal of Pest Science, 2014

ABSTRACT Aspergillus is a promising source of bioactive secondary metabolites. The addition of in... more ABSTRACT Aspergillus is a promising source of bioactive secondary metabolites. The addition of insect-derived material in the broth culture could trigger the biosynthesis of insecticidal and antimicrobial compounds by entomopathogenic fungus (EF). Insects inhabit diverse niches and interact with various bacteria, for this reason, when a fungus kills an insect should inhibit the insect?s gut bacteria to prevent the insect consumptionby bacteria. According with this hypothesis, the EF is able to produce substances that inhibit bacteria growth or bacteria virulence strategies. In the present, investigation is demonstrated that the addition of Tribolium castaneum Herbst (Coleoptera: Tenebrionidae) components (2 % w/v) in a culture of saprophytic fungus Aspergillus parasiticus MOR 3 induces the production of a novel compound present inside mycelium 2-(4-bromophenyl)-2-oxoethyl benzoate, that is reported here for the first time as a natural product of A. parasiticus. In addition, increased significantly the fungal extracellular production of undecyl 4-fluorobenzoate. The mycelium extract (ME) at 250 ìg per g of diet on adult population of T. castaneum produced an alteration of the feeding behavior of coleopteran insects (Repellency index = +30). The fraction derived from ME (Fr2) that contained the organobromine compound, 2-(4-bromophenyl)-2-oxoethylbenzoate had the highest inhibition of the Pseudomonas aeruginosa virulence factors like elastase enzyme (49 %) at 5 ìg/ml and biofilm formation (43 %) at 100 ìg/ml. The results suggest that the extract from mycelium of a non-aflatoxigenic A. parasiticus MOR 3 strain is a potential candidate as food coleopteran repellent as well as an anti-virulence strategy of P. aeruginosa.

Journal of Natural Products, 2010

Seven new prenylated acylphloroglucinols, yungensins A-G, were isolated from the diethyl ether ex... more Seven new prenylated acylphloroglucinols, yungensins A-G, were isolated from the diethyl ether extract of the scaly rhizomes and roots of an Argentine collection of the fern Elaphoglossum yungense. All the compounds contained a geranyl group attached to the filicinic acid-type residue. The diethyl ether extract and yungensins A-F displayed antibacterial effects against Staphylococcus aureus and Pseudomonas aeruginosa with MICs ranging from 10 to 200 μg/mL. All tested acylphloroglucinols, except for yungensin D, caused bacteriolysis of S. aureus at MBC or higher doses (50-200 μg/mL). At 10 μg/mL, the ether extract as well as yungensins A, B, and D-F altered the biofilm production in both microoganisms.

Journal of Natural Products, 2011

Journal of Environmental Management, 2013

The current trend of increasing air, water, and soil pollution is, in part, due to inadequate man... more The current trend of increasing air, water, and soil pollution is, in part, due to inadequate management of municipal solid waste (MSW). The relationship between public health and the collection, storage and improper disposal of solid waste has encouraged several studies and the results were attributed to the spread of over twenty human and animal diseases due to this interrelationship. The term "single cell protein" (SCP) refers to microbial biomass used as a dietary additive. It has high nutritional value because of its high content of vitamins, lipids, and proteins of biological quality (the presence of all essential amino acids) (Lal, 2005). The aim of this work was to design a culture media for microbiological assays and to produce SCP for animal feeding, using nutrients contained in organic waste. In order to compare the effectiveness of food waste (FW) and LAPTg media, different strains of Lactobacillus, Enterococcus, Staphylococcus, Shigella, Salmonella, Saccharomyces and Schizosaccharomyces were studied. In all cases, the growth obtained from FW and LAPTg culture media were not significantly different (p > 0.05). In addition, the growth of Saccharomyces cerevisiae was studied in order to produce SCP for animal feeding. Comparative experiments involving molasses broth, FW broth, and basal broth were carried out. The biomass yield calculated at 24 h from FW broth was 13% lower than from molasses broth. The FW broth provided a significantly lower biomass yield; however, it can be very useful in areas where molasses are not available. FW broth can be elaborated at low cost, in any populated region of the world because its ingredients are wastes generated by humans. It has great versatility, allowing the development of a wide variety of microorganisms, both Gram negative and Gram positive bacteria as well as yeasts. The production of safe protein additives, with high biological quality and low cost, is necessary due to the increasing global demand for food for humans and animals.

Brazilian Journal of Microbiology, 2012

The influence of seven phenolic compounds, normally present in wine, on the growth and arginine d... more The influence of seven phenolic compounds, normally present in wine, on the growth and arginine deiminase system (ADI) of Lactobacillus hilgardii X 1 B, a wine lactic acid bacterium, was established. This system provides energy for bacterial growth and produces citrulline that reacts with ethanol forming the carcinogen ethyl carbamate (EC), found in some wines. The influence of phenolic compounds on bacterial growth was compound dependent. Growth and final pH values increased in presence of arginine. Arginine consumption decreased in presence of protocatechuic and gallic acids (31 and 17%, respectively) and increased in presence of quercetin, rutin, catechin and the caffeic and vanillic phenolic acids (between 10 and 13%, respectively). ADI enzyme activities varied in presence of phenolic compounds. Rutin, quercetin and caffeic and vanillic acids stimulated the enzyme arginine deiminase about 37-40%. Amounts of 200 mg/L gallic and protocatechuic acids inhibited the arginine deiminase enzyme between 53 and 100%, respectively. Ornithine transcarbamylase activity was not modified at all concentrations of phenolic compounds. As gallic and protocatechuic acids inhibited the arginine deiminase enzyme that produces citrulline, precursor of EC, these results are important considering the formation of toxic compounds.

Annals of Microbiology, 2007

Abstract This work was carried out to determine the activity of enzymes involved in arginine meta... more Abstract This work was carried out to determine the activity of enzymes involved in arginine metabolism inLactobacillus plantarum isolated from wine and previously characterised at molecular level. The activity of the enzymes arginine deiminase and ornithine ...

Inorganica Chimica Acta, 2015

a b s t r a c t The reaction in alkaline aqueous solution between sulfamethoxazole (SMX) and mang... more a b s t r a c t The reaction in alkaline aqueous solution between sulfamethoxazole (SMX) and manganese (II) chloride produces colorless crystals with formula [Mn(H 2 O) 6 ] 0.5 [Mn(SMX) 3 ], which was characterized by UV-Vis, IR and Raman spectroscopy and thermal analysis. The crystal structure of [Mn(H 2 O) 6 ] 0.5 [Mn(SMX) 3 ] complex has been solved by X-ray diffraction methods. It crystallizes in the cubic Pa-3 space group with a = 19.5476(1) Å, and Z = 8 molecules per unit cell. [Mn(SMX) 3 ] À complex is at a crystallographic special position of C 3 symmetry with the Mn(II) ion cis-coordinated to three equivalent sulfamethoxazole molecules acting as bidentate ligands in a propeller-like conformation. [Mn(H 2 O) 6 ] 2+ hydrate ion is at crystal special position of S 6 symmetry with the metal in an almost perfect octahedral coordination with six water molecules. At 100 lg/mL Mn(H 2 O) 6 ] 0.5 [Mn(SMX) 3 ] and SMX, inhibited the Staphylococcus aureus biofilm formation by 92% and 54%, respectively. However, at the same concentration Mn(H 2 O) 6 ] 0.5 [Mn(SMX) 3 ] and SMX inhibited the planktonic bacterial growth by 52% and 81%, respectively. The Mn(II) complex inhibited the biofilm formation in values higher than 35% at the concentration 0.5 lg/mL. These results suggest that the metal complex [Mn(H 2 O) 6 ] 0.5 [Mn(SMX) 3 ] is a good candidate for the development of new antimicrobial agent acting in part as bactericidal but mainly as antipathogenic agent.

Journal of Natural Products, Nov 1, 2010

Seven new prenylated acylphloroglucinols, yungensins A-G, were isolated from the diethyl ether ex... more Seven new prenylated acylphloroglucinols, yungensins A-G, were isolated from the diethyl ether extract of the scaly rhizomes and roots of an Argentine collection of the fern Elaphoglossum yungense. All the compounds contained a geranyl group attached to the filicinic acid-type residue. The diethyl ether extract and yungensins A-F displayed antibacterial effects against Staphylococcus aureus and Pseudomonas aeruginosa with MICs ranging from 10 to 200 μg/mL. All tested acylphloroglucinols, except for yungensin D, caused bacteriolysis of S. aureus at MBC or higher doses (50-200 μg/mL). At 10 μg/mL, the ether extract as well as yungensins A, B, and D-F altered the biofilm production in both microoganisms.

Vii Jornadas Cientificas De Los Grupos De Investigacion Enologica Gienol Logrono 21 22 Y 23 De Mayo De 2003 2003 Isbn 84 8125 201 8 Pags 276 278, 2003

Sugarcane is rich in dietary fibers that have beneficial properties in gastrointestinal tract. In... more Sugarcane is rich in dietary fibers that have beneficial properties in gastrointestinal tract. In addition the sugarcane is well known for its protective role in mammalian health. However, the sugarcane industry produces vast quantities of residues. Sugarcane blunting (waste produced by the sugarcane industry) was fermented under different conditions: using native microbiota (control), using specific goat probiotics, including Lactobacillus reuteri DDL19 (Treatment Nº1), Lactobacillus alimentarius DDL 48 (Treatment Nº2), Enterococcus faecium DDE 39 (Treatment Nº3), Bifidobacterium bifidum DDBA (Treatment Nº4) and a probiotic mixture containing equal amounts of the four probiotics (Treatment N°5). The fermented sugarcane is a synbiotic supplement for goats because it contains goat probiotic as well as dietary fibers considered as prebiotic. Firstly, the ensilages were microbiologically examined and then administered simultaneously to different batches of six goats each immediately af...

Global Journal of Agricultural Innovation, Research & Development, 2014

Polygonum punctatum Elliot (water pepper) is a pungent herb ancestrally employed as a disinfectan... more Polygonum punctatum Elliot (water pepper) is a pungent herb ancestrally employed as a disinfectant in traditional medicine by Toba Indians of the north-eastern region of Argentina and also commonly used as spice in Japanese cuisine. GC-MS of whole diethyl ether extract (EE) from aerial parts allowed to identify 14 volatile terpenoids such as sesquiterpenes: α-bisabolol (3.4 %), polygodial and isotadeonal (34.0%); various methylated phenol like αtocopherol or vitamin E (3.6 %), and phytosterols: stigmasterol (2.1%) and β-sitosterol (29.9 %). Thus, water pepper is a promising source of drimane sesquiterpenes and phytoestrogens with important bioactivities.

Phytomedicine : international journal of phytotherapy and phytopharmacology, Jan 15, 2015

Five dammarane-type triterpenoids, five pentacyclic triterpenoids (three of them carrying a carbo... more Five dammarane-type triterpenoids, five pentacyclic triterpenoids (three of them carrying a carboxylic acid group), and two aromadendrane-type sesquiterpenoids were isolated from an Argentinian collection of the liverwort Lepidozia chordulifera. Compounds were characterized by comparison of their spectral data with those previously reported and tested in their ability to control bacterial growth, biofilm formation, bacterial Quorum Sensing process (QS), and elastase activity of Pseudomonas aeruginosa, as well as bacterial growth and biofilm formation of Staphylococcus aureus. The key role played by biofilm and elastase activity in bacterial virulence make them a potential target for the development of antibacterial agents. The aromadendrane-type sesquiterpenoid viridiflorol was the most potent biofilm formation inhibitor, producing 60% inhibition in P. aeruginosa and 40% in S. aureus at 50µg/ml. Ursolic and betulinic acids (two of the pentacyclic triterpenoids isolated) were able to...

Public Health Microbiology, 2004

Biogenic amines can be formed and degraded as a result of normal metabolic activity in animals, p... more Biogenic amines can be formed and degraded as a result of normal metabolic activity in animals, plants, and microorganisms and are usually produced by the decarboxylation of amino acids. Recent trends in food security are promoting an increasing search for trace compounds that can affect human health. Although they are present in fermented foods and beverages in low quantities, they exhibit interactions with normal human metabolism (e.g., having vasoactive or psychoactive properties) that justify the research on their presence in foods and the possible related toxicological effects that they may cause. Estimation of the biogenic amines histamine, tyramine, agmatine, putrescine, and cadaverine is important not only from the point of view of their toxicity, but also because they can be used as indicators of the degree of freshness or spoilage of food. Until recently, because of the difficulty in detecting and quantifying amines reliably we have had insufficient information about their occurrence in different types of foods and beverages. These problems are related to matrix interference (e.g., the presence of free amino acids) and the low levels at which the amines are found. Early techniques for the determination of biogenic amines in foods were based on thin-layer chromatography. More modern analytical techniques have since been developed that allow the acquisition of reliable quantitative data and better separation/resolution of various amines. The quantitative determination of biogenic amines is generally accomplished by overpressure-layer chromatography, high-performance liquid chromatography (HPLC), and gas chromatography. The use of reverse-phase column and precolumn derivatization was more efficient and faster than the conventional ion-exchange techniques. This study was conducted to evaluate two HPLC derivatization methods for quantitative determination of biogenic amines: the method described by Gonzales de Llano et al. for amino acid analysis and the method described by Eerola et al.

Food and Nutrition Sciences, 2013

Different peppers fermentations (Capsicum annum, grossum variety) were assayed: spontaneous, nati... more Different peppers fermentations (Capsicum annum, grossum variety) were assayed: spontaneous, native microflora supplemented individually with Lactobacillus plantarum N8, Leuconostoc mesentereroides L. or Pediococcus pentosaceus 12p and by pure or combined cultures of these lactic acid bacteria (LAB). In order to eliminate the native flora, different kinds of heat treatment were assayed. The treatment selected was heating in autoclaved after research 3/4 atmosphere and to turn off. Fermentations were carried out at 22˚C and 30˚C and the culture media contained 2% or 0.2% glucose and 4% NaCl. Sugar consumption, pH reduction and acid production were higher at 30˚C than at 22˚C. At both temperatures, spontaneous fermentation showed a slower rate reduction in pH than inoculated samples. Diminution in pH in presence of 2% glucose was faster than at 0.2%, but minimum pH was in both case lower than 3.0. Maximum growth was reached between 2 and 5 days of fermentation in all the samples assayed. After 30 days of incubation in presence of 2% glucose the survival of LAB was nearly 5 log ufc/ml. The survival was higher at the lower temperature assayed for both glucose concentrations. Organoleptic properties of peppers fermented with a mixed culture of Leuconostoc mesenteroides and Pediococcus pentosaceus were found best by a human panel. This sample has a relation lactic acid/acetic acid of nearly 3 in the conditions assayed.

Journal of Dairy Science, 2015

The effect of a mixture of potentially probiotic bacteria (MPPB; Lactobacillus reuteri DDL 19, La... more The effect of a mixture of potentially probiotic bacteria (MPPB; Lactobacillus reuteri DDL 19, Lactobacillus alimentarius DDL 48, Enterococcus faecium DDE 39, and Bifidobacterium bifidum strains) on the milk fatty acid (FA) profile, with emphasis on cis-9,trans-11 conjugated linoleic acid (CLA) in the middle stage of goat lactation, was determined. In addition, the effects of MPPB feeding on the FA profile in intestinal content and intestinal morphology in weaned goats were analyzed. The probiotic supplement was able to modify FA composition of milk and intestinal content. The unsaturated FA concentrations in milk (g of FA/L of milk) increased from 4.49 to 7.86 for oleic (18:1), from 0.70 to 1.39 for linoleic (18:2), from 0.063 to 0.187 for linolenic (18:3) acid, and from 0.093 to 0.232 for CLA. The atherogenicity index diminished 2-fold after MPPB ingestion. In the intestinal content of the weaned goats, no significant difference in saturated FA concentration compared with the control was observed. However, oleic acid, linolenic acid, CLA, and docosahexaenoic acid concentrations increased by 81, 23, 344, and 74%, respectively, after probiotic consumption. The ruminal production of CLA was increased by the MPPB. However, bacterial strains of MPPB were unable to produce CLA in culture media. By histological techniques, it was observed that the treated group had intestinally more conserved morphological structures than the control group. The results obtained in this study indicate that the MPPB administration in lactating and weaned goats allows for the production of milk with improved concentrations of beneficial compounds, and also produces a protective effect in the goat intestine. The results obtained in this study reinforce the strategy of probiotics application to enhance goat health with the production of milk with higher concentrations of polyunsaturated FA.

Handbook of Plant-Based Fermented Food and Beverage Technology, Second Edition, 2012

Anaerobe, 2014

The mutagen binding ability of the goat probiotics (Lactobacillus reuteri DDL 19, Lactobacillus a... more The mutagen binding ability of the goat probiotics (Lactobacillus reuteri DDL 19, Lactobacillus alimentarius DDL 48, Enterococcus faecium DDE 39, and Bifidobacterium bifidum DDBA) was evaluated. The oral administration of these probiotics reduced fecal mutagens and intestinal cancer markers in goats. Secondly, the effects of probiotics against the mutagenesis induced by sodium azide (SA), and Benzopyrene (B[α]P) by performing the modified Ames test using Salmonella typhimurium TA 100 was investigated. The capacity to bind benzopyrene and the stability of the bacterial-mutagen complex was analyzed by HPLC. The dismutagenic potential against both mutagens was proportional to probiotic concentration. Results showed that probiotic antimutagenic capacity against SA was ranging from 13 to 78%. The mixture of four goat probiotics (MGP) displayed higher antimutagenic activity against SA than any individual strains at the same cell concentration. This study shows that the highest diminution of mutagenicity in presence of B[α]P (74%) was observed in presence of MGP. The antimutagenic activity of nearly all the individual probiotic and the MGP were in concordance with the B[α]P binding determined by HPLC. According to our results, the B[α]P binding to probiotic was irreversible still after being washed with DMSO solution. The stability of the toxic compounds-bacterial cell binding is a key consideration when probiotic antimutagenic property is evaluated. MGP exhibits the ability to bind and detoxify potent mutagens, and this property can be useful in supplemented foods for goats since it can lead to the removal of potent mutagens and protect and enhance ruminal health and hence food safety of consumers.

Pharmaceutical Biology, 2015

Context: It is necessary to advance the field of alternative treatments for chronic wounds that a... more Context: It is necessary to advance the field of alternative treatments for chronic wounds that are financially accessible to the least economically developed countries. Previously we demonstrated that topical applications of Lactobacillus plantarum culture supernatants (LAPS) on human-infected chronic wounds reduce the pathogenic bioburden, the amount of necrotic tissue, and the wound area, as well as promote debridement, granulation tissue, and wound healing. Objective: To study LAPS chemically and biologically and to find potential molecules responsible for its pro-healing and anti-pathogenic properties in chronic wounds. Materials and methods: (1) Chemical analysis: extracts were subjected to a column chromatography and the fractions obtained were studied by GCMS. (2) Quantification: DL-lactic acid (commercial kit), phenolic compounds (Folin-Ciocalteu), H 2 O 2 (micro-titration), and cations (flame photometry). (3) Biological analysis: autoinducers type 2 (AI-2) (Vibrio harveyi BB170 bioassay), DNAase activity (Agar DNAase), and Pseudomonas aeruginosa biofilm inhibition (crystal violet technique). Results: According to its biological activity, the most significant molecules found by GCMS were the following: antimicrobials (mevalonolactone, 5-methyl-hydantoine, benzoic acid, etc.); surfactants (di-palmitin, distearin, and 1,5-monolinolein); anesthetics (barbituric acid derivatives), and AI-2 precursors (4,5-dihydroxy-2,3-pentanedione and 2-methyl-2,3,3,4tetrahydroxytetrahydrofurane). Concentrations measured (mg/mL): DL-lactic acid (11.71 ± 1.53) and H 2 O 2 (36 ± 2.0); phenolic compounds (485.2 ± 15.20); sodium (370 ± 17); potassium 920 ± 24); calcium (20 ± 4); and magnesium (15 ± 3). DNAase from LAPS had activity on genomic DNA from PMNs and P. aeruginosa. Discussion and conclusion: The molecules and biological activities found in LAPS could explain the observed effects in human chronic wounds.

Phytotherapy research : PTR, 2011

Acanthospermal B (AcB), the major sesquiterpene lactone (SL) of Acanthospermum hispidum, an herb ... more Acanthospermal B (AcB), the major sesquiterpene lactone (SL) of Acanthospermum hispidum, an herb widely spread in Argentina, is a selective antibacterial agent against Enterococcus faecalis and Staphylococcus aureus, but inactive on Gram-negative and Lactobacillus. Methicillin-resistant Staphylococcus aureus (MRSA) is one of the main microorganisms involved in human chronic infection. A balb\c mouse skin infection model was developed to reproduce the lesions caused by acute and chronic infections produced by MRSA. After determination of the maximum concentration of AcB unable to produce tissular injury after intradermal injection, the anti-MRSA effect of AcB was evaluated on skin, liver and spleen tissues of infected mice. AcB, at doses of 2.5 mg/kg, produced a ten times decrease of MRSA growth in skin infection. In addition, the same dose prevented the dissemination to liver and/or spleen. AcB also displayed a bacteriostatic effect, in vitro, on MRSA cultures at 50 µg/mL that seems...

Journal of the Science of Food and Agriculture, 2013

BACKGROUND: Lactobacillus hilgardii, a wine lactic acid bacterium, is able to use arginine, throu... more BACKGROUND: Lactobacillus hilgardii, a wine lactic acid bacterium, is able to use arginine, through the arginine deiminase pathway with the formation of citrulline, a precursor of the carcinogen ethyl carbamate. The influence of different Argentine wine varieties (Merlot, Cabernet Sauvignon and Malbec), on bacterial growth and arginine metabolism was examined. Furthermore, the effect of different components normally present in wines on the enzyme activities of the arginine deiminase system was determined.

Journal of Pest Science, 2014

ABSTRACT Aspergillus is a promising source of bioactive secondary metabolites. The addition of in... more ABSTRACT Aspergillus is a promising source of bioactive secondary metabolites. The addition of insect-derived material in the broth culture could trigger the biosynthesis of insecticidal and antimicrobial compounds by entomopathogenic fungus (EF). Insects inhabit diverse niches and interact with various bacteria, for this reason, when a fungus kills an insect should inhibit the insect?s gut bacteria to prevent the insect consumptionby bacteria. According with this hypothesis, the EF is able to produce substances that inhibit bacteria growth or bacteria virulence strategies. In the present, investigation is demonstrated that the addition of Tribolium castaneum Herbst (Coleoptera: Tenebrionidae) components (2 % w/v) in a culture of saprophytic fungus Aspergillus parasiticus MOR 3 induces the production of a novel compound present inside mycelium 2-(4-bromophenyl)-2-oxoethyl benzoate, that is reported here for the first time as a natural product of A. parasiticus. In addition, increased significantly the fungal extracellular production of undecyl 4-fluorobenzoate. The mycelium extract (ME) at 250 ìg per g of diet on adult population of T. castaneum produced an alteration of the feeding behavior of coleopteran insects (Repellency index = +30). The fraction derived from ME (Fr2) that contained the organobromine compound, 2-(4-bromophenyl)-2-oxoethylbenzoate had the highest inhibition of the Pseudomonas aeruginosa virulence factors like elastase enzyme (49 %) at 5 ìg/ml and biofilm formation (43 %) at 100 ìg/ml. The results suggest that the extract from mycelium of a non-aflatoxigenic A. parasiticus MOR 3 strain is a potential candidate as food coleopteran repellent as well as an anti-virulence strategy of P. aeruginosa.

Journal of Natural Products, 2010

Seven new prenylated acylphloroglucinols, yungensins A-G, were isolated from the diethyl ether ex... more Seven new prenylated acylphloroglucinols, yungensins A-G, were isolated from the diethyl ether extract of the scaly rhizomes and roots of an Argentine collection of the fern Elaphoglossum yungense. All the compounds contained a geranyl group attached to the filicinic acid-type residue. The diethyl ether extract and yungensins A-F displayed antibacterial effects against Staphylococcus aureus and Pseudomonas aeruginosa with MICs ranging from 10 to 200 μg/mL. All tested acylphloroglucinols, except for yungensin D, caused bacteriolysis of S. aureus at MBC or higher doses (50-200 μg/mL). At 10 μg/mL, the ether extract as well as yungensins A, B, and D-F altered the biofilm production in both microoganisms.

Journal of Natural Products, 2011

Journal of Environmental Management, 2013

The current trend of increasing air, water, and soil pollution is, in part, due to inadequate man... more The current trend of increasing air, water, and soil pollution is, in part, due to inadequate management of municipal solid waste (MSW). The relationship between public health and the collection, storage and improper disposal of solid waste has encouraged several studies and the results were attributed to the spread of over twenty human and animal diseases due to this interrelationship. The term "single cell protein" (SCP) refers to microbial biomass used as a dietary additive. It has high nutritional value because of its high content of vitamins, lipids, and proteins of biological quality (the presence of all essential amino acids) (Lal, 2005). The aim of this work was to design a culture media for microbiological assays and to produce SCP for animal feeding, using nutrients contained in organic waste. In order to compare the effectiveness of food waste (FW) and LAPTg media, different strains of Lactobacillus, Enterococcus, Staphylococcus, Shigella, Salmonella, Saccharomyces and Schizosaccharomyces were studied. In all cases, the growth obtained from FW and LAPTg culture media were not significantly different (p > 0.05). In addition, the growth of Saccharomyces cerevisiae was studied in order to produce SCP for animal feeding. Comparative experiments involving molasses broth, FW broth, and basal broth were carried out. The biomass yield calculated at 24 h from FW broth was 13% lower than from molasses broth. The FW broth provided a significantly lower biomass yield; however, it can be very useful in areas where molasses are not available. FW broth can be elaborated at low cost, in any populated region of the world because its ingredients are wastes generated by humans. It has great versatility, allowing the development of a wide variety of microorganisms, both Gram negative and Gram positive bacteria as well as yeasts. The production of safe protein additives, with high biological quality and low cost, is necessary due to the increasing global demand for food for humans and animals.

Brazilian Journal of Microbiology, 2012

The influence of seven phenolic compounds, normally present in wine, on the growth and arginine d... more The influence of seven phenolic compounds, normally present in wine, on the growth and arginine deiminase system (ADI) of Lactobacillus hilgardii X 1 B, a wine lactic acid bacterium, was established. This system provides energy for bacterial growth and produces citrulline that reacts with ethanol forming the carcinogen ethyl carbamate (EC), found in some wines. The influence of phenolic compounds on bacterial growth was compound dependent. Growth and final pH values increased in presence of arginine. Arginine consumption decreased in presence of protocatechuic and gallic acids (31 and 17%, respectively) and increased in presence of quercetin, rutin, catechin and the caffeic and vanillic phenolic acids (between 10 and 13%, respectively). ADI enzyme activities varied in presence of phenolic compounds. Rutin, quercetin and caffeic and vanillic acids stimulated the enzyme arginine deiminase about 37-40%. Amounts of 200 mg/L gallic and protocatechuic acids inhibited the arginine deiminase enzyme between 53 and 100%, respectively. Ornithine transcarbamylase activity was not modified at all concentrations of phenolic compounds. As gallic and protocatechuic acids inhibited the arginine deiminase enzyme that produces citrulline, precursor of EC, these results are important considering the formation of toxic compounds.