János Dégi | University of Life Sciences "King Michael I" from Timisoara (original) (raw)
Papers by János Dégi
All pet animals have a specific microbial and parasitary flora wich, in certain conditions, may e... more All pet animals have a specific microbial and parasitary flora wich, in certain conditions, may expose humans to the zoonotic risk.
A survey of feline herpesvirus type 1 (FHV-1) conjunctivitis, in domestic cats from Timiş County ... more A survey of feline herpesvirus type 1 (FHV-1) conjunctivitis, in domestic cats from Timiş County area in westhern Romania was conducted in between Ianuary and September 2011, and we compared the results with results in other country, published by other researchers, investigated by immunofluorescent antibody assay (IFA). The positive rate of FHV-1 conjunctivitis in domestic cats were 25%. Seroprevalence of FHV-1 in studied domestic cats, with clinical signs were 45%.
Veterinary Sciences
Staphylococcal infection treatment in dogs is frequently associated with adverse side effects, hi... more Staphylococcal infection treatment in dogs is frequently associated with adverse side effects, high costs, prolonged treatment, and resistant strain selection. Staphylococcus aureus is the most frequently isolated staphylococci in cases of canine superficial pyoderma. The number of Staphylococcus strains to exhibit primary resistance to various drugs in vitro is increasing. Propolis has a diverse chemical composition and well-known therapeutic properties against bacterial infections. The current investigation evaluated in vitro the antimicrobial activity of the commercial allopathic antimicrobials, Romanian propolis ethanolic extracts, against clinical Staphylococcus aureus strains isolated from superficial dermatitis clinical samples in dogs and two reference strains: Staphylococcus aureus ATCC 25923 and Staphylococcus aureus ATCC 43300, as the MSSA and MRSA positive controls, respectively, in western Romania. We used the microdilution broth technique to evaluate the susceptibility...
Journal of Biotechnology, 2012
A total of 84 diarrheic fecal samples were collected from dairy calves (1-60 days old) from seven... more A total of 84 diarrheic fecal samples were collected from dairy calves (1-60 days old) from seven different farms, located in three western counties of Romania. Samples were previously examined under light microscopy through direct smear technique for the presence of Cryptosporidium oocysts. Genomic DNA was extracted from Cryptosporidium positive samples (n = 12) and species were determined by a two step nested polymerase chain reaction of the small subunit rRNA gene (18S), followed by RFLP analysis of the secondary PCR products with SspI, VspI and MboII enzymes. Digestions with endonucleases revealed characteristic bands for Cryptosporidium parvum (10 samples; 83.3%) and Cryptosporidium bovis (1 sample; 8.3%). One isolate (8.3%) was found to contain both species. This is the first genetic identification of Cryptosporidium bovis in calves from Romania.
Journal of Biotechnology, 2012
Stray dogs have long been regarded as a potential source of zoonotic diseases (bacterial zoonotic... more Stray dogs have long been regarded as a potential source of zoonotic diseases (bacterial zoonotic risk) for human. In particular, host zoonotic bacteria and parasites in the intestine of dogs were found to pose a significant risk to human health. An ensemble social change, economic and environmental, across the globe, reflects on epidemiological characteristics and pathogenesis of diseases and pathogens. And the development and supervision of bacterial zoonosis, with particular reference to multiple antibiotic resistant staphylococci isolated from dog faces, were important changes, which we refer in this study.In fecal samples from dogs were isolated Staphylococcus aureus strains pathogenic to man (MRSA), so proving dog faeces role in urban areas as a reservoir of bacteria with multiple resistance. Because the genes coding for antibiotic resistance can be transmitted between bacteria and contact between pets and their owners is tighter than in the past, our study suggests that conta...
This study was conducted to investigate the prevalence of zoonotic staphylococci isolated from do... more This study was conducted to investigate the prevalence of zoonotic staphylococci isolated from dogs in Western Romania. Samples were obtained from adult dogs of both sexes submitted to the University Veterinary Clinics Timisoara and private veterinary practice. Animals selected for this study had no known history of previous antibiotic treatment. Samples were identified and labeled as to source, male or female, adult and the anatomical area of harvesting. A total of 51 samples were obtained from different anatomical sites such as nose, eyes, ears, extremities reproductive and skin. After growth, staphylococcal isolates were identied according to their characteristics as outlined in Bergey’s Manual of Determinative Bacteriology and the Manual of Clinical Microbiology. 35 samples were positive for staphylococci, being isolated both positive and coagulase-negative species. The species most frequently isolated were S. (pseud)intermedius, S. aureus, S. hycus, S. epidermidis and S. haemo...
The present study aimed to determine the prevalence of Staphylococcus species, which pose risks f... more The present study aimed to determine the prevalence of Staphylococcus species, which pose risks for public health, by evaluating skin samples collected from dogs in an animal shelter in Timisoara. Skin samples were taken from 78 dogs, which were either clinically healthy or suffering from dermatological conditions. Staphylococcus spp. was isolated and recognized based on conventional methods based on colony appearance, microscopic morphology, sugar fermentation, and coagulase activity. Following biochemical analysis, Staphylococcus isolates were subject to PCR tests to detect sa-f and sa-r genes to confirm the isolates to genus level. The typical colonies were identified to species level using biochemical methods, namely the VITEK®2 ID-GP64 identification card (bioMerieux, France). The phenotypic antimicrobial resistance profiling was performed using the VITEK®2 AST GP Gram-positive specific bacteria card (bioMerieux, France). Forty-three samples were confirmed as positive for Staph...
Treating infections caused by Pseudomonas aeruginosa is increasingly difficult due to high antibi... more Treating infections caused by Pseudomonas aeruginosa is increasingly difficult due to high antibiotic resistance, materialized through the presence of multiple resistance strains, as well as due to rapid development of resistance throughout treatment. The present survey was conducted to investigate the antibiotic susceptibility profile of Pseudomonas aeruginosa pathogens in two University Veterinary hospitals from different geographical regions of Romania (i.e., Southwest Timișoara county and Northeast Iași county) involved in superficial canine infections. A total of 142 swab specimens were collected from dogs with superficial infections (superficial skin infections, otitis externa, and perianal abscess) to assess the presence of Pseudomonas aeruginosa, based on phenotypic and molecular characterization. According to their confirmed morphological and molecular features, 58 samples (40.84%; 58/142) were positive for Pseudomonas aeruginosa (according to their confirmed morphological ...
Antibiotics
The present study was undertaken to investigate the presence of Salmonella spp. in the faeces of ... more The present study was undertaken to investigate the presence of Salmonella spp. in the faeces of client-owned cats in urban areas and to evaluate the risk that is posed to public health. Fresh faecal samples were collected directly from the rectums from 53 diarrhoeic and 32 non-diarrhoeic cats. The samples were individually screened for the presence of Salmonella spp. using standard methods and, in the case of positive findings, the resulting typical colonies were then biochemically confirmed using the VITEK®2 automated system. Subsequently, all of the Salmonella spp. isolates were molecularly tested for the presence of the invA gene. All of the isolates were serotyped using the slide agglutination technique according to the White–Kauffmann–Le Minor scheme. The phenotypic antimicrobial susceptibility profile of the isolated strains was obtained from the VITEK®2 system using specific cards from the Gram-negative bacteria. A total of 16 of the samples (18.82%) tested positive for Salm...
Antibiotics
Excessive use of antimicrobials and inadequate infection control practices has turned antimicrobi... more Excessive use of antimicrobials and inadequate infection control practices has turned antimicrobial resistance (AMR) into a global, public health peril. We studied the expression of qnrA, qnrB, and qnrS plasmid in ciprofloxacin (CIP)-resistant strains of Escherichia coli in swine and humans from Romania, using the Polymerase Chain Reaction (PCR) technique. Antibiotic Susceptibility Testing (AST) for human subjects (H) on 147 samples and 53 swine (S) was ascertained as well as the isolation of bacterial DNA (E. coli) as follows: bacteriolysis, DNA-binding, rinsing, elution, amplification, and nucleic acids’ migration and U.V. visualization stages. From 24 samples of E. coli resistant to CIP collected from H subjects and 15 from S, for PCR analysis, 15 H and 12 S were used, with DNA purity of 1.8. The statistically analyzed results using the Crosstabs function (IBM SPSS Statistics-Ver. 2.1.), revealed the qnrS (417 bp) gene in 13 human subjects (52.0%), as well as in all swine samples...
All pet animals have a specific microbial and parasitary flora wich, in certain conditions, may e... more All pet animals have a specific microbial and parasitary flora wich, in certain conditions, may expose humans to the zoonotic risk.
A survey of feline herpesvirus type 1 (FHV-1) conjunctivitis, in domestic cats from Timiş County ... more A survey of feline herpesvirus type 1 (FHV-1) conjunctivitis, in domestic cats from Timiş County area in westhern Romania was conducted in between Ianuary and September 2011, and we compared the results with results in other country, published by other researchers, investigated by immunofluorescent antibody assay (IFA). The positive rate of FHV-1 conjunctivitis in domestic cats were 25%. Seroprevalence of FHV-1 in studied domestic cats, with clinical signs were 45%.
Veterinary Sciences
Staphylococcal infection treatment in dogs is frequently associated with adverse side effects, hi... more Staphylococcal infection treatment in dogs is frequently associated with adverse side effects, high costs, prolonged treatment, and resistant strain selection. Staphylococcus aureus is the most frequently isolated staphylococci in cases of canine superficial pyoderma. The number of Staphylococcus strains to exhibit primary resistance to various drugs in vitro is increasing. Propolis has a diverse chemical composition and well-known therapeutic properties against bacterial infections. The current investigation evaluated in vitro the antimicrobial activity of the commercial allopathic antimicrobials, Romanian propolis ethanolic extracts, against clinical Staphylococcus aureus strains isolated from superficial dermatitis clinical samples in dogs and two reference strains: Staphylococcus aureus ATCC 25923 and Staphylococcus aureus ATCC 43300, as the MSSA and MRSA positive controls, respectively, in western Romania. We used the microdilution broth technique to evaluate the susceptibility...
Journal of Biotechnology, 2012
A total of 84 diarrheic fecal samples were collected from dairy calves (1-60 days old) from seven... more A total of 84 diarrheic fecal samples were collected from dairy calves (1-60 days old) from seven different farms, located in three western counties of Romania. Samples were previously examined under light microscopy through direct smear technique for the presence of Cryptosporidium oocysts. Genomic DNA was extracted from Cryptosporidium positive samples (n = 12) and species were determined by a two step nested polymerase chain reaction of the small subunit rRNA gene (18S), followed by RFLP analysis of the secondary PCR products with SspI, VspI and MboII enzymes. Digestions with endonucleases revealed characteristic bands for Cryptosporidium parvum (10 samples; 83.3%) and Cryptosporidium bovis (1 sample; 8.3%). One isolate (8.3%) was found to contain both species. This is the first genetic identification of Cryptosporidium bovis in calves from Romania.
Journal of Biotechnology, 2012
Stray dogs have long been regarded as a potential source of zoonotic diseases (bacterial zoonotic... more Stray dogs have long been regarded as a potential source of zoonotic diseases (bacterial zoonotic risk) for human. In particular, host zoonotic bacteria and parasites in the intestine of dogs were found to pose a significant risk to human health. An ensemble social change, economic and environmental, across the globe, reflects on epidemiological characteristics and pathogenesis of diseases and pathogens. And the development and supervision of bacterial zoonosis, with particular reference to multiple antibiotic resistant staphylococci isolated from dog faces, were important changes, which we refer in this study.In fecal samples from dogs were isolated Staphylococcus aureus strains pathogenic to man (MRSA), so proving dog faeces role in urban areas as a reservoir of bacteria with multiple resistance. Because the genes coding for antibiotic resistance can be transmitted between bacteria and contact between pets and their owners is tighter than in the past, our study suggests that conta...
This study was conducted to investigate the prevalence of zoonotic staphylococci isolated from do... more This study was conducted to investigate the prevalence of zoonotic staphylococci isolated from dogs in Western Romania. Samples were obtained from adult dogs of both sexes submitted to the University Veterinary Clinics Timisoara and private veterinary practice. Animals selected for this study had no known history of previous antibiotic treatment. Samples were identified and labeled as to source, male or female, adult and the anatomical area of harvesting. A total of 51 samples were obtained from different anatomical sites such as nose, eyes, ears, extremities reproductive and skin. After growth, staphylococcal isolates were identied according to their characteristics as outlined in Bergey’s Manual of Determinative Bacteriology and the Manual of Clinical Microbiology. 35 samples were positive for staphylococci, being isolated both positive and coagulase-negative species. The species most frequently isolated were S. (pseud)intermedius, S. aureus, S. hycus, S. epidermidis and S. haemo...
The present study aimed to determine the prevalence of Staphylococcus species, which pose risks f... more The present study aimed to determine the prevalence of Staphylococcus species, which pose risks for public health, by evaluating skin samples collected from dogs in an animal shelter in Timisoara. Skin samples were taken from 78 dogs, which were either clinically healthy or suffering from dermatological conditions. Staphylococcus spp. was isolated and recognized based on conventional methods based on colony appearance, microscopic morphology, sugar fermentation, and coagulase activity. Following biochemical analysis, Staphylococcus isolates were subject to PCR tests to detect sa-f and sa-r genes to confirm the isolates to genus level. The typical colonies were identified to species level using biochemical methods, namely the VITEK®2 ID-GP64 identification card (bioMerieux, France). The phenotypic antimicrobial resistance profiling was performed using the VITEK®2 AST GP Gram-positive specific bacteria card (bioMerieux, France). Forty-three samples were confirmed as positive for Staph...
Treating infections caused by Pseudomonas aeruginosa is increasingly difficult due to high antibi... more Treating infections caused by Pseudomonas aeruginosa is increasingly difficult due to high antibiotic resistance, materialized through the presence of multiple resistance strains, as well as due to rapid development of resistance throughout treatment. The present survey was conducted to investigate the antibiotic susceptibility profile of Pseudomonas aeruginosa pathogens in two University Veterinary hospitals from different geographical regions of Romania (i.e., Southwest Timișoara county and Northeast Iași county) involved in superficial canine infections. A total of 142 swab specimens were collected from dogs with superficial infections (superficial skin infections, otitis externa, and perianal abscess) to assess the presence of Pseudomonas aeruginosa, based on phenotypic and molecular characterization. According to their confirmed morphological and molecular features, 58 samples (40.84%; 58/142) were positive for Pseudomonas aeruginosa (according to their confirmed morphological ...
Antibiotics
The present study was undertaken to investigate the presence of Salmonella spp. in the faeces of ... more The present study was undertaken to investigate the presence of Salmonella spp. in the faeces of client-owned cats in urban areas and to evaluate the risk that is posed to public health. Fresh faecal samples were collected directly from the rectums from 53 diarrhoeic and 32 non-diarrhoeic cats. The samples were individually screened for the presence of Salmonella spp. using standard methods and, in the case of positive findings, the resulting typical colonies were then biochemically confirmed using the VITEK®2 automated system. Subsequently, all of the Salmonella spp. isolates were molecularly tested for the presence of the invA gene. All of the isolates were serotyped using the slide agglutination technique according to the White–Kauffmann–Le Minor scheme. The phenotypic antimicrobial susceptibility profile of the isolated strains was obtained from the VITEK®2 system using specific cards from the Gram-negative bacteria. A total of 16 of the samples (18.82%) tested positive for Salm...
Antibiotics
Excessive use of antimicrobials and inadequate infection control practices has turned antimicrobi... more Excessive use of antimicrobials and inadequate infection control practices has turned antimicrobial resistance (AMR) into a global, public health peril. We studied the expression of qnrA, qnrB, and qnrS plasmid in ciprofloxacin (CIP)-resistant strains of Escherichia coli in swine and humans from Romania, using the Polymerase Chain Reaction (PCR) technique. Antibiotic Susceptibility Testing (AST) for human subjects (H) on 147 samples and 53 swine (S) was ascertained as well as the isolation of bacterial DNA (E. coli) as follows: bacteriolysis, DNA-binding, rinsing, elution, amplification, and nucleic acids’ migration and U.V. visualization stages. From 24 samples of E. coli resistant to CIP collected from H subjects and 15 from S, for PCR analysis, 15 H and 12 S were used, with DNA purity of 1.8. The statistically analyzed results using the Crosstabs function (IBM SPSS Statistics-Ver. 2.1.), revealed the qnrS (417 bp) gene in 13 human subjects (52.0%), as well as in all swine samples...