Effect of doxycycline and Lactobacillus probiotics on mRNA expression of ABCC2 in small intestines of chickens (original) (raw)
Related papers
Bulgarian Journal of Veterinary Medicine, 2018
Poultry feed is often supplemented by Lactobacillus probiotics which may alter drug bioavailability by affecting the expression of intestinal ATP-binding cassette (ABC) efflux transporters. Therefore the effect of probiotics, administered alone or in combination with enrofloxacin, on the expression of ABCB1, ABCC2 and ABCG2 mRNAs in chickens was evaluated. Day-old Ross chicks (n=24) were divided in four equal groups. Control group was not treated. The second group received feed with addition of probiotics Lactobacillus brevis, L. plantarum and L. bulgaricus 5 days after hatching, for 15 days. The third group received probiotics as described above and enrofloxacin at the age of 15 days (10 mg/kg, via drinking water for 5 days). The last group received enrofloxacin at age of 15 days (10 mg/kg, via drinking water for 5 days). Expression of ABC transporters in liver, duodenum and jejunum was determined by qRT-PCR. Down-regulation of ABCG2 mRNA in the liver (P<0.05), its up-regulation in the duodenum (P<0.05) and increased ABCB1 mRNA levels in the jejunum (P<0.05) can be attributed to enrofloxacin treatment. Decrease in ABCC2 mRNA expression in the duodenum can be associated with enrofloxacin administration. The observed changes were related to enrofloxacin administration and to lesser extent to Lactobacillus supplementation.
2017
The function of immune system of poultry has a significant impact on poultry husbandry sustainability. Therefore the aim of this study was to investigate the effect of lactic acid bacteria administered with enrofloxacin or doxycycline on expression levels of antimicrobial peptide cathelicidin-3 (CATH3) at mRNA level in the duodenum, jejunum and liver of broilers. A day-old Ross (n=24) and Duc (n=24) chickens were included in experiments with enrofloxacin and doxycycline, respectively. They were divided into four groups (n=6) for each experiment: control, supplemented with probiotics (15 days via feed, 5 days after hatching), treated with either enrofloxacin or doxycycline (10 mg.kg for 5 days, via drinking water) and treated with antibiotic and probiotics. Expression levels of CATH3 mRNA in liver, duodenum and jejunum were determined by RT-PCR and were statistically evaluated by Mann-Whitney test.Administration of probiotics led to insignificant down-regulation of CATH3 mRNA in the ...
Enrofloxacin and Probiotic Lactobacilli Influence PepT1 and LEAP-2 mRNA Expression in Poultry
Probiotics and Antimicrobial Proteins, 2016
Expression of peptide transporter 1 (PepT1) and liver-expressed antimicrobial peptide 2 (LEAP-2) in chickens can be influenced by food deprivation, pathological conditions and drug administration. Effect of three putative probiotic Lactobacillus strains and enrofloxacin on the expression of PepT1 and LEAP-2 mRNA was investigated in Ross 308 chickens. One-day-old chicks (n = 24) were allocated to following groups: control (without treatment); group treated with probiotics via feed; group treated with a combination of probiotics and enrofloxacin; and a group given enrofloxacin only. The drug was administered at a dose of 10 mg kg-1 , via drinking water for 5 days. Samples from liver, duodenum and jejunum were collected 126 h after the start of the treatment. Expression levels of PepT1 and LEAP-2 were determined by real-time polymerase chain reaction and were statistically evaluated by Mann-Whitney test. Enrofloxacin administered alone or in combination with probiotics provoked a statistically significant up-regulation of PepT1 mRNA levels in the measured organ sites. These changes can be attributed to a tendency of improvement in utilization of dietary peptide and in body weight gain. LEAP-2 mRNA expression levels did not change significantly in enrofloxacin-treated chickens in comparison with control group.
Journal of Animal Physiology and Animal Nutrition, 2018
The purpose of this study was to verify the ability of a probiotic in the feed to maintain the stability of the gut microbiota in chickens after antibiotic therapy and its association with growth performance. One thousand six hundred twenty 1-day-old Cobb male were housed in floor pens (36 pens, 45 birds/pen) and were fed corn-/ soya bean meal-based diets supplemented with or without probiotic (Bacillus subtilis) during the entire rearing phase. From 21 to 24 days of age (three consecutive days), the chickens were submitted to antibiotic therapy via drinking water (bacitracin and neomycin) in order to mimic a field treatment and induce dysbiosis. Growth performance was monitored until 42 days of age. At 2, 4 and 6 days after antibiotic therapy, three chickens from each pen were euthanized and the contents of the small intestine and caeca were collected and pooled. The trial was conducted with four treatments and nine replicates in a 2 × 2 factorial arrangement for performance characteristics (with and without probiotic × with and without antibiotic therapy); for the intestinal microbiota, it was in a 2 × 2 × 3 factorial arrangement (with and without probiotic × with and without antibiotic therapy × 2, 4 and 6 days after the antibiotic therapy) with three replicates per treatment. Terminal restriction length polymorphism (T-RFLP) analysis showed that the structure of gut bacterial community was shaped by the intestinal segment and by the time after the antibiotic therapy. The number of 16S rDNAs copies in caecum contents decreased with time after the therapeutic treatment. The antibiotic therapy and dietary probiotic supplementation decreased richness and diversity indexes in the caecal contents. The improved performance observed in birds supplemented with probiotic may be related to changes promoted by the feed additive in the structure of the intestinal bacterial communities and phylogenetic groups. Antibiotic therapy modified the bacterial structure, but did not cause loss of broiler performance.
Microorganisms
The reduction of antimicrobial resistance is a major challenge for the scientific community. In a few decades, infections by resistant bacteria are forecasted to be the main cause of death in the world. The withdrawal of antibiotics as growth promoters and their preventive use in animal production is essential to avoid these resistances, but this may impair productivity and health due to the increase in gut inflammation. This reduction in productivity aggravates the problem of increasing meat demand in developing countries and limits the availability of raw materials. Probiotics are promising products to address this challenge due to their beneficial effects on microbiota composition, mucosal barrier integrity, and immune system to control inflammation. Although many modes of action have been demonstrated, the scientific community is not able to describe the specific effects that a probiotic should induce on the host to maximize both productivity and animal health. First, it may be ...
Animals, 2020
The aim of this study was to compare the effect of the use of enrofloxacin and a probiotic containing Enterococcus faecium and Bacillus amyloliquefaciens strains in the first week of life of chickens on oxidative and epigenetic changes in molecules and intestinal integrity. The three treatments were as follows: the control group received no additive in the drinking water (GC); the second group (GP) received a probiotic preparation in the drinking water during the first five days of life, providing E. faecium strain 4a1713 at 1.0 × 107 CFU/L water and B. amyloliquefaciens 4b1822 at 1.0 × 107 CFU/L water, the third group (GA) received an antibiotic (enrofloxacin 0.5 mL/L water) in the drinking water during the first five days of life. The use of both enrofloxacin and a probiotic containing E. faecium and B. amyloliquefaciens strains in chickens’ first week of life improved intestinal integrity and reduced inflammation and oxidative and epigenetic changes in the small intestine. This e...
Probiotic Supplementation in Poultry Production as an Alternative to Antibiotic Feed Additive
Animal Review, 2019
Provision of safe and healthy poultry products to the human being is the prime objective of the poultry industry, but one should keep in mind the welfare of animals and reverence for the environment. Meat and eggs demand also increasing with the increase in world human population. To meet this increasing demand for meat and eggs, antibiotics growth promoters were used world widely. After the ban on antibiotics growth promoters due to its residual effects on human health, nutritionists are focusing on novel growth promoters those without causing productivity loss, or product quality has augmented. Therefore, probiotics are a safe alternative to antibiotics and its beneficial effects proven from many decades. Live microorganisms are used to deliver health benefits to chicken, usually by reestablishing or improving the intestinal microbiota. Probiotics have a beneficial effect on weight gain, immunity, meat traits and somewhat impact on gut health of poultry. The subject of review paper will be worthwhile meant for researchers and poultry nutritionists to advance their understanding of alternatives to antibiotics in poultry production without affecting performance and the welfare of chicken. Contribution/Originality: This study is important in regards to antibiotic resistance in human being coming from animal products. Antibiotic resistance is one of the biggest public health challenges of our time. Each year so many people get an antibiotic-resistant infection, and many people die. Fighting this threat is a public health priority that requires a collaborative global approach across sectors. So, this study will give direction to researchers and food producers to avoid from antibiotics harmful effects to animals and as well as human being. 1. INTRODUCTION Poultry business recognized as one of the rapidly growing section among different agriculture sectors. Consumption of meat and eggs is increasing due to easy availability, comparatively reasonable price and also rich in entire important nutrients which could cover the deficiencies of essential food amino acids, minerals, and vitamins Dhama, et al. [1]; Ajit, et al. [2]. Rinttilä and Apajalahti [3] reported that poultry intestinal health has been investigated and studied extensively for the higher production performance of chicken, as the intestine is an important site where nutrient absorption takes place. While the gut is the main organ which exposed more to pathogens microorganisms after skin [4]. For this purpose, since 1940 antibiotics were used in animal feed as Animal Review
EffEct of probIotIcS on protEIn productIon In f AttEnInG chIckEn mEA t
2009
in this work the influence of probiotic preparations added into the water or feed on protein production in the thigh and the breast muscle of chickens was observed. Probiotics were applied into drink water for the Hubbard Jv (the 1 st experiment) and Hybro (the 2 nd experiment) chickens and into the feed of crossbred roSS 308 chickens (the 3 rd experiment). Probiotics were produced on the base of microorganisms of Lactobacillus fermentum (1. experiment), Enterococcus faecium (2. experiment) and Bacillus subtilis and Bacillus licheniformis (3. experiment). Time of feeding was 42 days. A feed-stuff was used either in the dry form (the 1 st and the 2 nd experiment) or in granular form (the 3 rd experiment). The nutritive contents were the same in each experiment, except the 3 rd experiment, which was designed on the plant base. Probiotics increased the protein content in the breast muscle of all crossbreds, but this increase was not statistically significant. in the thigh muscle, prote...