Chemical constituent with cytotoxicity from Sterculia diversifolia (original) (raw)
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Triterpenoids and coumarins from the leaves of Sterculia foetida Linn
Science and Technology Development Journal, 2020
Introduction: Sterculia foetida Linn. is widely distributed in tropical countries. As the continuous study on the hexane and ethyl acetate extracts of Sterculia foetida leaves, the isolation and structural determination of four triterpenoids and two coumarins were addressed. Method: The crude extract was prepared from dried power of Sterculia foetida leaves by maceration method in ethanol. This extract was then separated by liquid-liquid partition with n-hexane, chloroform, and ethyl acetate, respectively, to obtain the corresponding extracts. The hexane and ethyl acetate extracts were applied to multiple silica gel column chromatography to yield six compounds. Their chemical structures were determined by the NMR data analysis as well as the comparison their spectroscopic data and physical properties with those of reported literature. Results: Four triterpenoid compounds, including betulinic acid (1), conyzasaponin G (2), taraxerol (3), and taraxer-14-ene- 1a,3b -diol (4), and two c...
Sterculia diversifolia bears anti-cancer and immunomodulatory activities
Bangladesh Journal of Pharmacology, 2017
The present study was aimed to evaluate the methanolic extract and subsequent solvents soluble fractions of Sterculia diversifolia bark for cytotoxic, anti-cancer and immunomodulatory activities. Phytochemical investigation confirmed the presence of alkaloids, flavonoids, saponins, glycosides, etc. In the cytotoxic activity, n-hexane showed potent activity (LD50: 7.0 µg/mL) followed by dichloromethane fraction (LD50: 16.2 µg/mL). In the anti-cancer activity, dichloromethane fraction showed potent activity (IC50: 5.9 µg/mL) followed by ethyl acetate fraction (IC50: 9.5 µg/mL). While in the immunomodulatory assay, ethyl acetate fraction showed a very significant activity (IC50: 21.0 µg/mL) followed by dicloromethane and n-butanol fractions (IC50: 25.0 and 25.3 µg/mL respectively). Hence, it is clear that S. diversifolia has anti-cancer and immunomodulatory agents.
Sterculia diversifolia bears anti-cancer and immunomodulatory activities
Bangladesh Journal of Pharmacology, 2017
The present study was aimed to evaluate the methanolic extract and subsequent solvents soluble fractions of Sterculia diversifolia bark for cytotoxic, anticancer and immunomodulatory activities. Phytochemical investigation confirmed the presence of alkaloids, flavonoids, saponins, glycosides, etc. In the cytotoxic activity, n-hexane showed potent activity (LD50: 7.0 µg/mL) followed by dichloromethane fraction (LD50: 16.2 µg/mL). In the anti-cancer activity, dichloromethane fraction showed potent activity (IC50: 5.9 µg/mL) followed by ethyl acetate fraction (IC50: 9.5 µg/mL). While in the immunomodulatory assay, ethyl acetate fraction showed a very significant activity (IC50: 21.0 µg/mL) followed by dicloromethane and n-butanol fractions (IC50: 25.0 and 25.3 µg/mL respectively). Hence, it is clear that S. diversifolia has anticancer and immunomodulatory agents.
Pharmacological activity investigation of Sterculia coccinea Nuzhat Mahbub
2019
Sterculia coccinea plant extract used to assess its different biological activity. Extract was made by soaking the dried plant powder in methanol. After comparing with the standard we found that Methanol extract of the sample gave the activity against all the experimented microbes of ZI (zone of inhibition) against E. coli and B. subtillis. After performing the antioxidant, thrombolytic, antidiarrheal, hypoglycemic and cytotoxic activity assay of methanol extract of sample plant we saw that it has a good biological activity that can be used as a potential traditional medicine.
The Journal of Animal and Plant Sciences, 2020
Genus Sterculia possess various types of compounds which have therapeutic potential. This study was aimed to explore the anticonvulsant, CNS depressant, analgesic, anti-inflammatory and anti-pyretic activities of Sterculia diversifolia stem bark and leaves extracts in mice. The secondary metabolites in MESD (methanolic extract of Sterculia diversifolia) was characterized by UV, IR, 1 H-NMR, 13 C NMR and 2D-NMR spectra in combination with EI-MS spectrometric techniques. Anticonvulsant and CNS depressant effect of MESD was assessed using strychnine-induced convulsion and phenobarbitone-induced hypnotic protocol. Analgesic activity was performed by acetic acid induced writhing and hot plate test while anti-inflammatory and anti-pyretic activities were assessed by carrageenan induced right hind paw edema and brewer's yeast induced pyrexia. The isolation of compounds with different elucidation techniques (1 H-NMR, 13 C NMR and 2D-NMR spectra in combination with EI-MS spectrometric techniques) led to luteolin, isoquercitrin and ursolic acid. The current study results showed that crude MESD significantly (p < 0.05) delayed the onset, shortened the duration and offered protection against strychnine-induced convulsion. Crude MESD significantly (p < 0.05) prolonged the time of phenobarbitone-induced hypnosis. The analgesic response of crude MESD stem bark was more significant as compare to crude MESD leaves, while crude MESD also showed significant anti-inflammatory and antipyretic activity through dose dependent phenomenon. These significant results might be due to flavonoids (luteolin, isoquercitrin) and triterpenoids (ursolic acid) presence, though the involvement of other biologically active secondary metabolites cannot be overlooked.
Plants
Sterculia foetida, also known as jangli badam in Bangladesh, is a traditionally used plant that has pharmacological activities. A qualitative phytochemical analysis was performed to assess the metabolites in a methanolic extract of S. foetida seeds (MESF), and the cytotoxic, thrombolytic, anti-arthritics, analgesic, and antipyretic activities were examined using in vitro, in vivo, and in silico experiments. Quantitative studies were performed through gas chromatography-mass spectroscopy (GC-MS) analysis. The brine shrimp lethality bioassays and clot lysis were performed to investigate the cytotoxic and thrombolytic activities, respectively. The anti-arthritics activity was assessed using the albumin denaturation assay. Analgesic activity was determined using the acetic acid-induced writhing test and the formalin-induced paw-licking test. A molecular docking study was performed, and an online tool was used to perform ADME/T (absorption, distribution, metabolism, and excretion/toxicit...
A new coumarin from the fruits of Coutarea hexandra
Natural Product Research, 2007
A new 5-O-β-D-glucopyranosyl-4-(4-hydroxyphenyl)-7-methoxy-2H-chromen-2-one (1), together with four known compounds, one coumarin, 5-O-β-D-galactopyranosyl-4-(4-hydroxyphenyl)-7-methoxy-2H-chromen-2-one (2) and three cucurbitacins, 23,24-dihydrocucurbitacin F (3), 23,24-dihydro-25-acetylcucurbitacin F (4) and 2-O-β-D-glucopyranosyl-23,24-dihydrocucurbitacin F (5) have been isolated and characterised from the ethanol extract of Coutarea hexandra fruits. Their structures have been established by spectroscopic analysis (NMR and MS). Interpretation of the HMQC, HMBC, COSY-45 and NOESY experiments permitted us to establish stereochemistry of the natural products. All compounds were tested in cytotoxicity assays against the breast (MCF-7), lung (H-460), and central nervous system (SF-268) human cancer cell lines.
GC-MS Analysis, Metal Analysis and Antimicrobial Investigation of Sterculia diversifolia
Pharmaceutical Chemistry Journal, 2020
Plants of Sterculia genus contain various types of bioactive compounds which bears therapeutic potential. The current research was planned to perform gas chromatography-mass spectrometry (GC-MS) analysis and metal analysis, and to test the antimicrobial activity of various extracted samples of Sterculia diversifolia G. Don specie. Screening of S. diversifolia for chemical constituents was carried out using various qualitative procedures, which confirmed the existence of a number of bioactive compounds. Gas chromatography followed by GC-MS is a significant analytical tool for the assessment of composition of fixed oils or oily fractions. Atomic absorption spectrophotometer was used for the determination of micronutrients, while atomic emission flame photometer for the analysis of macronutrients. The antibacterial and antifungal activities were investigated by well diffusion and tube dilution techniques respectively. The n-hexane fractions of both the stem bark and leaves of S. diversifolia were analyzed with the help of GC-MS, resulting in the identification of 32 and 62 compounds, respectively. Various micronutrients and macronutrients shown reasonable quantities in stem bark and leaves extracts. The n-hexane and dichloromethane fractions of S. diversifolia stem bark, leaves and seeds showed mild to moderate range of antibacterial activity against Staphylococcus aureus and no antifungal activity against any fungal strain. Our findings support the use of S. diversifolia in traditional medicine.
Synthesis & Pharmacological Activity of Cytotoxicity Assay of Coumarins SVCM1-SVCM7
The therapeutic properties of natural and synthetic compounds against human illnesses have attracted a lot of interest. The pharmacological and biological effects of coumarins are widely exploited in medicine; they include anti-inflammatory, anticoagulant, antihypertensive, anticonvulsant, antioxidant, antibacterial, and neuroprotective properties. Signaling pathways affecting many cellular functions may also be modulated by coumarin derivates. Whether or not a coumarin has pharmacological, biochemical, or therapeutic characteristics is dependent on the nature of the replacement surrounding the coumarin core structure. The capacity to kill, repel, or otherwise impact the development of Anopheles arabiensis mosquitoes was evaluated using seven synthetic halogenated coumarins (SVCM1-SVCM7). The compounds SVCM1-SVCM7 were evaluated for their bactericidal and fungicidal activities using the disc diffusion method.