Standardization and quantification of curcumin from Curcuma longa extract using UV visible spectroscopy and HPLC (original) (raw)
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Curcuminoid in Curcuma longa extract
Analysis of individual and total curcuminoid composed of curcumin (CUR), desmethoxycurcumin (DMCUR) and bisdemethoxycurcumin (BDMCUR) in Curcuma species is very important. Curcuminoids are frequently used as markers in herbal medicine involving the use of Curcuma species in its formulation. The objective of this study was to validate high-performance liquid chromatography (HPLC) and Fourier transform infrared spectroscopy-partial least square (FTIR-PLS) for quantitative analysis of total and individual curcuminoids in turmeric (Curcuma longa L.) extract. The turmeric powder was macerated using ethanol and evaporated to obtain an ethanolic extract. The actual contents of individual curcuminoids of CUR, DMCUR, and BDMCUR were determined using HPLC. FTIR-PLS can be used for quantitative analysis of individual and total curcuminoids using specific wavenumbers. The coefficient of determination (R 2 ) for the relationship between actual values and FTIR-PLS predicted values for calibration, internal, and external validation was >0.98 which indicated good accuracy. The relatively low values of errors in calibration, validation and external validation indicated good precision. FTIR spectroscopy-PLS can be used as an alternative technique over HPLC for determination of individual and total curcuminoid in turmeric extracts.
Research Journal of Phytochemistry, 2015
A simple and isocratic liquid chromatographic using UV detection at wavelength of 425 nm have been validated and used for quantitative analysis of curcumin in ethanolic extract of turmeric (Curcuma longa Linn.) and Curcuma xanthorriza (Zingiberaceae), indigenous to Java region, Indonesia. The method was optimized for separation of three curcuminoids namely curcumin, demethoxycurcumin and bisdemethoxycurcumin using Waters Xterra MS C18 column (4.6×250 mm, 5 μm). The mobile phase used consisted of aquabidestilata and acetonitrile (65:35 v/v) containing 1% acetic acid. The analytical method was validated in terms of linearity, sensitivity, precision and accuracy. The developed method was linear over the curcumin concentration range of 10-60 μg mLG 1 with correlation coefficient value of 0.999. The precision of the developed method expressed as Relative Standard Deviation (RSD) value was in the range of 0.17-1.17% for three different levels of sample. The recoveries obtained for the accuracy assessment were 8.50-101.23%. The sensitivity of analytical method was expressed with Limit of Detection (LoD) and Limit of Quantification (LoQ). The values of LoD and LoQ were 1.13 and 3.34 μg mLG 1 , respectively. The method was successfully used for quantitative analysis of curcumin in the rhizomes of Curcuma longa and Curcuma xanthorriza. The levels of curcumin found in those rhizomes are in the range of 3.03±0.01-7.31±0.02 (C. longa) and in the range of 1.69±0.02-4.92±0.01 (C. xanthorriza).
Asian Pacific Journal of Tropical Biomedicine, 2012
India Objective: To develop a simple, sensitive, precise, and accurate stability-indicating high performance thin-layer chromatographic method for analysis of curcumin (the main active constituent of turmeric). Methods: The separation was achieved on TLC aluminum plates precoated with silica gel 60F 254 using toluene-chloroform-methanol (5:4:1, v/v/v) as a mobile phase. Densitometric analysis was performed at 430 nm. Results: This system was found to have compact spot of curcumin at RF value of (0.31暲0.02). For the proposed procedure, linearity (r2 = 0.99354 暲 0.00120), limit of detection (50 ng/spot), limit of quantification (200 ng/spot), recovery (ranging from 98.35%-100.68%), and precision (曑2.25%) were found to be satisfactory. Statistical analysis reveals that the content of curcumin in different geographical region varied significantly. Conclusions: The highest and lowest concentration of curcumin in Turmeric was found to be present in sample of Erode (Tamilnadu) and Surat (Gujrat) respectively which inferred that the variety of turmeric found in Erode (Tamilnadu) is much superior to other region of India.
Journal of Pharmacognosy and Phytochemistry, 2020
Curcuma longa (turmeric) is a medicinal plant belonging to the family Zingiberaceae. It is widely used for the health care of humans, livestock and poultry. Turmeric is a potent antioxidant, anti-inflammatory, antimutagenic, antimicrobial, and anticancer agent. Curcumin is a polyphenol extracted from roots of turmeric. In the present study, fresh roots of Curcuma longa was collected from Herbal Garden, Ethno Veterinary Herbal Product Research and Development Centre, Tamil Nadu Veterinary and Animal Sciences University, Orathanadu, Thanjavur District, Tamil Nadu and evaluated for the identification and relative quantification of curcumin by using a simple, sensitive and accurate High Performance Thin Layer Chromatography method.
Research Journal of Medicinal Plant, 2015
FTIR spectroscopy in combination with multivariate calibration of Partial Least Square (PLS) has been developed for quantification of curcumin in the ethanolic extracts of Curcuma longa Linn and Curcuma xanthorriza Roxb. The optimization was done by selecting the best wavenumbers regions capable of providing the high coefficient of determination (R 2) and low values of Root Mean Square Error of Calibration (RMSEC). Finally, wavenumbers region of 2000-950 cmG 1 was selected for prediction of curcumin in the extracts. The correlation between actual values of curcumin determined by HPLC and FTIR predicted values using FTIR spectroscopy combined with PLS in ethanolic extract of C. longa and C. xanthorriza at 2000-950 cmG 1 revealed R 2 values of 0.96 and 0.99, respectively. The RMSEC values obtained are 0.299 and 0.089 for C. longa and C. xanthorriza, respectively. The high value of R 2 and low value of RMSEC indicated the high accuracy and precision of FTIR spectroscopy for quantification of curcumin in the extracts. These results indicated that FTIR spectroscopy combined with PLS is an alternative technique for determination of curcumin in Curcuma species. The developed method (FTIR spectroscopy) is rapid, no sample preparation and not involving excessive solvents and reagents.
Journal of chromatographic science, 2015
Cucuma longa Linn. (Fam-Zingiberaceae) is a valued medicinal plant contains curcuminoids (curcumin, demethoxycurcumin and bisdemethoxycurcumin) as major bioactive constituents. Previously reported analytical methods for analysis of curcuminoids were found to suffer from low resolution, lower sensitivity and longer analytical times. In this study, a rapid, sensitive, selective high-throughput ultra high performance liquid chromatography-tandem mass spectrometry (UPLC/Q-TOF-MS) method was developed and validated for the quantification of curcuminoids with an aim to reduce analysis time and enhance efficiency. UPLC/Q-TOF-MS analysis showed large variation (1.408-5.027% w/w) of curcuminoids among different samples with respect to their occurrence of metabolite and their concentration. The results showed that Erode (south province) contains highest quantity of curcuminoids and concluded to be the superior varieties. The results obtained here could be valuable for devising strategies for ...
Current Science
The action of turmeric depends on three curcuminoids: curcumin, demethoxycurcumin and bisdemethoxycurcumin, whose distribution is highly varied among cultivars. A sensitive method for estimation of all three curcuminoids is essential for quality control. We developed a HPLC-MS method with lowest limits of detection and quantification of curcuminoids. Mass spectrometry (MS) authenticated the identity of curcuminoids. Principal component analysis of curcuminoids established the high variation among the selected seven cultivars of Curcuma, as well as commercial powders. We suggest that our HPLC method can be used for quality control of turmeric.