Curcuminoid in Curcuma longa extract (original) (raw)
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Current Science
The action of turmeric depends on three curcuminoids: curcumin, demethoxycurcumin and bisdemethoxycurcumin, whose distribution is highly varied among cultivars. A sensitive method for estimation of all three curcuminoids is essential for quality control. We developed a HPLC-MS method with lowest limits of detection and quantification of curcuminoids. Mass spectrometry (MS) authenticated the identity of curcuminoids. Principal component analysis of curcuminoids established the high variation among the selected seven cultivars of Curcuma, as well as commercial powders. We suggest that our HPLC method can be used for quality control of turmeric.
Journal of chromatographic science, 2015
Cucuma longa Linn. (Fam-Zingiberaceae) is a valued medicinal plant contains curcuminoids (curcumin, demethoxycurcumin and bisdemethoxycurcumin) as major bioactive constituents. Previously reported analytical methods for analysis of curcuminoids were found to suffer from low resolution, lower sensitivity and longer analytical times. In this study, a rapid, sensitive, selective high-throughput ultra high performance liquid chromatography-tandem mass spectrometry (UPLC/Q-TOF-MS) method was developed and validated for the quantification of curcuminoids with an aim to reduce analysis time and enhance efficiency. UPLC/Q-TOF-MS analysis showed large variation (1.408-5.027% w/w) of curcuminoids among different samples with respect to their occurrence of metabolite and their concentration. The results showed that Erode (south province) contains highest quantity of curcuminoids and concluded to be the superior varieties. The results obtained here could be valuable for devising strategies for ...
Journal of Pharmacognosy and Phytochemistry, 2018
Curcuma longa (Turmeric) is used successfully in Ayurvedic formulations from ancient times. It is a rich source of bioactive phytoconstituents like curcuminoids, turmerone and many more. Curcuminoids is the group of chief dynamic components and has number of medicinal uses such as anti-inflammatory, anti-HIV, antitumour, antiviral, anticancer, antifungal and antiparasitic. Different analytical methods have been developed in recent year for the quality control analysis of curcuminoids in Curcuma longa extract including HPLC, HPTLC and UV-Visible Spectrophotometry. While the primary component curcumin from curcuminoids is still lacking for its analytical method development along with validation. Therefore, in the present study, a simple UV visible and HPLC method was developed and validated according to international conference harmonization (ICH) guidelines for the quantitative estimation of curcumin in Curcuma longa extract.
Asian Pacific Journal of Tropical Biomedicine, 2012
India Objective: To develop a simple, sensitive, precise, and accurate stability-indicating high performance thin-layer chromatographic method for analysis of curcumin (the main active constituent of turmeric). Methods: The separation was achieved on TLC aluminum plates precoated with silica gel 60F 254 using toluene-chloroform-methanol (5:4:1, v/v/v) as a mobile phase. Densitometric analysis was performed at 430 nm. Results: This system was found to have compact spot of curcumin at RF value of (0.31暲0.02). For the proposed procedure, linearity (r2 = 0.99354 暲 0.00120), limit of detection (50 ng/spot), limit of quantification (200 ng/spot), recovery (ranging from 98.35%-100.68%), and precision (曑2.25%) were found to be satisfactory. Statistical analysis reveals that the content of curcumin in different geographical region varied significantly. Conclusions: The highest and lowest concentration of curcumin in Turmeric was found to be present in sample of Erode (Tamilnadu) and Surat (Gujrat) respectively which inferred that the variety of turmeric found in Erode (Tamilnadu) is much superior to other region of India.
Acta Horticulturae, 2008
In Thailand, turmeric (Curcuma longa) is mainly used in the forms of capsules/tablets of the powder for herbal medicine while its extract is also used in herbal cosmetics and functional food. Thus the quality assessment of this plant is needed to control the limits of volatile oil and curcuminoids contents. This study was undertaken to evaluate total curcuminoid content in the ethanolic turmeric extract of C. longa rhizome collected from ten locations from the North, Northeast, Central and South of Thailand by a UV spectrophotometry and HPLC. Each curcuminoid content (curcumin, demethoxycurcumin and bisdemethoxycurcumin) determined by HPLC was also reported. By a UV spectrophotometer, the total curcuminoids content of all extracts was found within the limits of 14.14 ± 0.87% to 26.76 ± 0.17% w/w. By HPLC, the contents of curcumin, demethoxycurcumin, bisdemethoxycurcumin and total curcuminoids were found in the range of 8.55 ± 0.42% to 15.88 ± 0.46%, 1.50 ± 0.14% to 4.16 ± 0.17%, 5.54 ± 0.23% to 9.33 ± 0.30%, and 16.93 ± 0.63% to 29.37 ± 0.90% w/w of the extract, respectively. This indicates that the ethanol extract of C. longa should contain not less than 16% w/w total curcuminoids when determined by HPLC and not less than 13% w/w when determined by UV spectrophotometry. The samples from the South where rain is obtained all year were found to contain the highest amount of total and individual curcuminoid. The result confirms that turmeric grown in the South of Thailand is the best source for high contents of individual and total curcuminoids. This information would be a useful database for medicinal plant of the country, to maximize the potential of local community, especially for C. longa which is a "champion product" of Thailand. The results are also useful as a guide for further standardization of turmeric extracts used for pharmaceutical purposes.
Research Journal of Medicinal Plant, 2015
FTIR spectroscopy in combination with multivariate calibration of Partial Least Square (PLS) has been developed for quantification of curcumin in the ethanolic extracts of Curcuma longa Linn and Curcuma xanthorriza Roxb. The optimization was done by selecting the best wavenumbers regions capable of providing the high coefficient of determination (R 2) and low values of Root Mean Square Error of Calibration (RMSEC). Finally, wavenumbers region of 2000-950 cmG 1 was selected for prediction of curcumin in the extracts. The correlation between actual values of curcumin determined by HPLC and FTIR predicted values using FTIR spectroscopy combined with PLS in ethanolic extract of C. longa and C. xanthorriza at 2000-950 cmG 1 revealed R 2 values of 0.96 and 0.99, respectively. The RMSEC values obtained are 0.299 and 0.089 for C. longa and C. xanthorriza, respectively. The high value of R 2 and low value of RMSEC indicated the high accuracy and precision of FTIR spectroscopy for quantification of curcumin in the extracts. These results indicated that FTIR spectroscopy combined with PLS is an alternative technique for determination of curcumin in Curcuma species. The developed method (FTIR spectroscopy) is rapid, no sample preparation and not involving excessive solvents and reagents.
Nondestructive determination of curcuminoids from turmeric powder using FT-NIR
2010
Nondestructive method of measuring curcuminoid content of turmeric powder was developed by fourier transform near infrared (FT-NIR) spectrometer. Also, the calibration models describing the relationship between curcuminoid content and the NIR spectra of the turmeric powder were developed and evaluated. To develop the model, NIR reflectance spectra were acquired for each turmeric powder sample. Spectra were pre-processed by applying different spectra correction algorithms. The relationship between spectrometric curcuminoid content and FT-NIR spectra of turmeric powder was examined through partial least square (PLS) regression method in the wave number range of 6102 to 4246 cm −1. The root mean square error of cross-validation and test set validation were 0.104 and 0.201 whereas the determination coefficient (R 2) for both the validations were 97.64 and 87.53, respectively. A low value of root mean square error and high value of R 2 indicates a high correlation between the concentration and spectral data. Thus the FT-NIR offers a viable alternative to spectroscopic procedures as well as the method is time and cost effective for determination of curcuminoid content of turmeric.
Validated method for estimation of curcumin in turmeric powder
Curcumin, the active molecule present in Curcuma longa is known for its antitumour, antioxidant, antiarthritic, antiamyloid, anti-ischemic, anti-inflammatory activities. In addition, it may also be effective in treating malaria, prevention of cervical cancer, etc. An efficient, sensitive, and precise high-performance thin-layer chromatography (HPTLC) method has been developed for determination of curcumin in several marketed spices sample of turmeric powder and has been compared with an in-house turmeric powder. The HPTLC separation was performed on precoated aluminium backed HPTLC plates of 0.2 mm layer thickness with silica gel 60 F 254 with dichloromethane and methanol (99:1) combination as mobile phase. The plate was developed up to 80 mm at temperature of 20 ± 4 o C with 10 min. of chamber saturation. Under this condition the retardation factor (R f ) of curcumin is 0.43 and the compound was quantified at its absorbance maxima (λ max ) at 427 nm. The limit of detection and limit of quantification were found to be 49 ng and 148 ng per spot, respectively. The response of curcumin was linear over the range of 0.8 µg to 1.3 µg per spot with correlation coefficient 0.99395 indicates good relationship between peak area versus concentration. Recovery values from 99.60 to 99.73 % showed that the reliability and reproducibility of the method were excellent. The proposed method may be useful as an accurate, simple, cost effective and sensitive for quantitative estimation of curcumin.