Dittrichia graveolens(L.)GreuterEssential Oil: Chemical Composition, Multivariate Analysis, and Antimicrobial Activity (original) (raw)
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2011
The aim of the study was to survey the chemical composition, antimicrobial and antioxidant activities of Dittrichia graveolens essential oil in in vitro conditions. GC-FID and GC-MS analyses were performed to evaluate the chemical composition of essential oil. The antimicrobial activity against different kinds of microorganisms was determined by microbroth dilution assay. The antioxidant activity was evaluated by DPPH free radical scavenging system. Studies of chemical composition of essential oil revealed the presence of borneol (38.2%) and bornyl acetate (14.9%) as major constituents. Essential oil showed strong antimicrobial activity against Enterococcus faecalis isolates and Bacillus cereus. Staphylococcus aureus and Escherichia coli are less sensitive to the oil. The strong antimicrobial activity of oil against clinical isolates of bacteria was demonstrated. In addition, D. graveolens oil notably reduced the concentration of DPPH free radical with higher efficacy than that of t...
2011
The aims of present study were to analysis of chemical compositions and evaluate anti-Candida activity of Dittrichia gravolence essential oil. The composition of the hydrodistilled essential oil from aerial parts of D. graveolens (L.) Greuter were grown in Iran investigated by gas chromatography-mass spectroscopy (GC/ MS). The major components, among the identified 22, were 1,8 Cineol (54.89%), P-Cymen (16.2%), [beta]-pinene (6.94%) and Borneol (5.44%). Anti-Candida activity of the volatile oil of the aerial parts of D. gravolence against different isolates of Candida albicans was studied in vitro. Anti-Candida activity was done using serial dilutions of volatile oil in Sabouraud's dextrose agar (SDA). Agar well diffusion methods was used for detection minimum inhibitory concentration (MIC). MIC for 10 isolates of tested yeasts was 30.675 mg/ml.
Jordan Journal of Biological Sciences, 2021
The chemical characterization and antimicrobial activities of Dittrichia viscosa (L.) essential oils extracted by hydrodistillation technique from leaves and stems are reported in this work. Dittrichia viscosa (L.) samples were collected from the Bainem forest in the northwest part of Algiers (the capital). Gas chromatography-mass spectrometry (GC-MS) analytical method was employed to identify the oil's chemical composition. It was found that leaves are mainly composed of three major abundant composites, specifically, caryophyllene oxide (10.4%), fokienol (9.6%) and trans-nerolidol (7%). Moreover, the oil isolated from the stems was found to be chiefly composed of trans-totarol (18.1%), α-cedrol (16.7%), and ferruginol (16.6%). Additionally, antimicrobial activity tests were performed on the isolated essential oils using the zone of inhibition (agar disk-diffusion method) to determine the minimum inhibitory concentration (MIC) of four bacteria strains, mainly, Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli. The MIC values of leaves are found to range between 15 µg and 100 µg, whereas stems are found to exhibit MIC values ranging between 20 µg and 300 µg. Furthermore, antifungal susceptibility tests, which become important tools to dictate the treatment of fungal diseases, are conducted on two yeast strains: Saccharomyces cerevisiae and Candida albicans. The obtained antimicrobial results are correlated with the chemical composition findings of the essential oils from leaves and stem to determine the roles of the chemical composites on the antibacterial activity. Interestingly, the oil obtained from the leaves displayed a better inhibitory effect on (bacteria and yeast strains) in comparison with oil (stems). This difference in inhibitory effect can be attributed to the dominant existence of the oxygenated sesquiterpenes and trans-nerolidol compounds in leaves.
2003
Dittrichia viscosa subsp. viscosa (Compositae) is found on edges, wood clearings and in waste places of the Iberian Peninsula. Aerial parts of D. viscosa were collected at flowering phase in September-October 2001 around Lisbon, Portugal and the essential oils isolated by hydro-distillation for 4 h using a Clevenger-type apparatus. The oils were analyzed by gas chromatography and gas chromatography-mass spectrometry. Preliminary examination of the essential oils allowed the identification of 32 components. Only four components reached percentages over 5%: fokienol (11.8%), T-muurorol (7.9%), (E)-nerolidol (5.5%) and δ-cadinene (5.0%). The essential oils were tested against Helicobacter pylori and Listeria monocytogenes. Essential oils did not have antimicrobial activity against L. monocytogenes. The essential oil at 0.88 to 22.22 µg.ml-1 did not inhibit the growth of H. pylori, affected the growth slightly at 44.40 µg.ml-1 , and completely inhibited the growth at 88.80 to 133.20 µg.ml-1. Results show that use of D. viscosa essential oil in the treatment of gastric disorders caused by H. pylori can be effective.
Plants, 2021
With the increasing interest in obtaining biologically active compounds from natural sources, Dittrichia viscosa (L.) Greuter (Asteraceae) came into our focus as a readily available and aromatic wild shrub widely distributed in the Mediterranean region. This work provides a phytochemical profile of D. viscosa in terms of parallel chemical composition in the lipophilic fraction (essential oil) and the water fraction (hydrosol). GC-MS analysis identified 1,8-cineole, caryophyllene oxide, α-terpenyl acetate, and α-muurolol as the major components of the essential oil, while in the hydrosol p-menth-1-en-9-ol, 1,8-cineole, linalool, cis-sabinene hydrate, and α-muurolol were the major volatile components. 3,4-Dihydroxybenzoic acid was found to be the predominant compound in the hydrosol composition by HPLC analysis. The antimicrobial potential of both extracts was evaluated against thirteen opportunistic pathogens associated with common skin and wound infections and emerging food spoilage...
Chemical Compositions of the Essential Oil from the Jordanian Medicinal Plant Dittrichia Viscosa
2010
The chemical composition of the essential oil obtained from the aerial parts at the flowering stage of Dittrichia viscosa (L.) growing in Jordan was analyzed by gas chromatography and gas chromatography-mass spectrometry. The analysis of the essential oil led to the identification of 47 components. The main constituents were fokienol (20.87%), (E)-nerolidol (19.75%), β-Eudesm-6-en-4α-ol (5.64%), α-Vetivone (3.60%), α-Eudesmol (2.68%) and Caryophyllene oxide (2.57%).
Chemical Composition and Antimicrobial Activity of Diterpene and Essential
2015
Objective: The objective of the present study was to isolate and determine diterpene compound and essential oils from Hedychium roxburghii Blume rhizome and investigated those antimicrobial activities. Methods: The essential oils were obtained by steam distillation method, the residual was then extracted by reflux with ethanol. The content of essential oils was analyzed by gas chromatography-mass spectrometry (GC/MS) method. Ethanolic residual-distillation extract was concentrated then used to isolate compound 1 by vacuum liquid chromatography and centrifugal chromatography. It was characterized by infrared spectrophotometry, 1H-nuclear magnetic resonance (NMR), 13C-NMR, heteronuclear single quantum coherence-NMR, heteronuclear multiple bond correlation-NMR and carbon coupling13C-NMR. The antimicrobial activity of essential oils, ethanolic residual-distillation extract and compound 1 were carried out by microdilution method. Results: The oils exhibited antimicrobial activity against...
Molecules
Since some synthetic insecticides cause damage to human health, compounds in plants can be viable alternatives to conventional synthetic pesticides. Dittrichia viscosa L. is a perennial Mediterranean plant known to possess biological activities, including insecticidal properties. The chemical composition of an essential oil (EOD) from D. viscosa, as well as its antioxidant, antimicrobial, and insecticidal effects on the cowpea weevil (Callosobruchus maculatus) were determined. Forty-one volatile compounds were identified in EOD, which accounted for 97.5% of its constituents. Bornyl acetate (41%) was a major compound, followed by borneol (9.3%), α-amorphene (6.6%), and caryophyllene oxide (5.7%). EOD exhibited significant antioxidant activity in all tests performed, with an IC50 of 1.30 ± 0.05 mg/mL in the DPPH test and an EC50 equal to 36.0 ± 2.5 mg/mL in the FRAP assay. In the phosphor-molybdenum test, EOD results ranged from 39.81 ± 0.7 to 192.1 ± 0.8 mg AAE/g E. EOD was active on...
African Journal of Traditional, Complementary and Alternative medicines
Background: Dracocephalum kotschyi Boiss is a herb with widespread applications. Lorestan traditional healers have applied it for the treatment of rheumatoid diseases and stomach disorders. Materials and methods: Hydrodistillation process was used for essential oil extraction, the extracted essential oil was then analyzed through combination of capillary GC-FID, GC-MS and RI. The in vitro antimicrobial, antioxidant and cytotoxic activities of this essential oil were examined. Results indicate that the essential oil has a broad range of antimicrobial activity against all of the tested microorganisms. Results: The 50% of cytotoxic concentrations was 26.4 μg/ml and 4266.7 μg/ml for Hela cells and human lymphocytes, respectively. The oil cytotoxicity against the human tumor cell line was far higher than the amount required for human healthy cells. Conversely, the essential oil's IC 50 value of 49.2 μg/ml in the DPPH assay, could be regarded as its strong antioxidant potential. Conclusion: According to the data obtained, it can be concluded that D. kotschyi essential oil could be applied as a safe antibacterial and antioxidant agent for food and pharmaceutical purposes.