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2020
This research determined the effects of Citrus sinensis and Lycopersicon esculentum Miller juices on the hematological parameters of Rattus albus. To achieve its objectives, the study utilized quantitative-experimental method. For this purpose, nine, two-weeks-old male albino rat weighing 100-150g were used. Rats were kept under room temperature in a 12-hour alternate light and dark cycle and provided with free access to water and foods. Rats were divided into three equal groups namely the control group and experimental groups administered with 2ml of orange and 2ml of tomato juice respectively for 6 days for three continuous weeks. Blood were collected through saphenous vein sampling technique starting at the end of acclimatization and every 7th day of the week. The obtained blood samples were used for determination of hematocrit, red blood cell (RBC) count, hemoglobin, platelet count, white blood cell (WBC) and differential count including neutrophil, lymphocyte, monocytes, eosino...
Haematopoetic effect of methanol seed extract of Citrus paradisi Macfad (grape fruit) in Wistar rats
The present study was undertaken to evaluate the blood-forming effects of (100% methanol seed extract) of Citrus paradisi Macfad in adult Wistar rats for 30 days as a way of evaluating its traditional use in the treatment of blood deficiencies. Acute oral toxicity study was also conducted using limit dose test of the Up and Down Procedure statistical program (AOT425PgmStat, Version 1.0) at a dose of 2000 mg/kg body weight/oral route. Results showed significant (p<0.05) progressive and dose dependent elevations in total leucocyte count (TLC), lymphocyte differentials (Lymph.), red blood count (RBC), haemoglobin concentration (Hb), packed cell count (PCV), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC) and platelet count (PL). Reversed effect was recorded for the neutrophil (Neutro.) and monocyte (Mono.) differentials which were significantly (p<0.05) decreased in the treated rats. Acute oral toxicity showed the extract to be relatively safe at 2000 mg/kg on acute exposure. Thus, the overall results lend support to its folkloric use.
2021
Anaemia is a haematological disorder characterized by reduced red blood cells (RBCs) or haemoglobin (HB) or lowered ability of blood to carry oxygen leading to increased risk of morbidity and mortality. The anti-anaemic potential of aqueous extracts of Hibiscus sabdariffa seeds (HSS) was evaluated in this study. One-hundred and eighty (180) adult albino rats were divided into twelve groups with three replicates of 5 rats each (n =15) and designated as Group 1 (normal control), Group 2 (positive control, treated with standard drug), Group 3 (negative control, untreated) and Groups 4-6 (treated with 200, 400 and 600 mg.kg-1 of aqueous extracts of raw, HSS respectively), Groups 7-9 (treated with 200, 400 and 600 mg.kg-1 of aqueous extracts of boiled HSS respectively) and Groups 10-12 (treated with 200, 400 and 600 mg.kg-1 of aqueous extracts of fermented HSS respectively). The acute toxicity test revealed no toxic effect of HSS at 5000 mg.kg-1 and haemolytic anaemia was induced with 350 mg.kg-1 b.w of Zidovudine. After 28 days of extract administration, the animals were humanely sacrificed and blood samples collected for haematological analysis using Automated Haematology Analyzer. RBC count, HB concentration and packed cell volume (PCV) decreased significantly (p<0.05) after Zidovudine induction, but the aqueous extracts of raw, boiled and fermented HSS significantly (p<0.05) increased HB, RBC, PCV, white blood cells (WBC) and neutrophil levels when compared with negative control. The results suggest that aqueous extracts of HSS attenuated hematological indices by increasing RBC and haemoglobin production and could be utilized in management of anaemia.
Treatment of Experimental Hemolytic Anemia by Some Antioxidant Drugs
Hemolytic anemia is caused by abnormal breakdown of red blood cells (RBCs) either in the blood vessels (intravascular hemolysis) or elsewhere in the body (extravascular). The aim of this work was to investigate the efficacy of treatment with four antioxidant drugs containing Silymarin available in Egyptian market, in experimental anemia model of rat induced by single dose of phenylhydrazine (80 mg/kg) ip. The induction of anemia led to a significant decrease in hemoglobin level, red blood cells count, and haematocrit percent, and there was an increase in serum iron concentration, total iron binding capacity and percent of transferrin saturation when compared to their corresponding control. Oral administration of four preparations containing antioxidants to rats for 2 weeks resulted in a significant increase of hemoglobin level, red blood cells count, and haematocrit percent. There was also a decrease in serum iron concentration, total iron binding capacity and percent of transferrin...
Haematological indices of albino rats fed diets supplemented with jackfruit seed (S), bulb (B) or seedbulb blend (SB) were assessed. Diets were compounded to include 10%, 30%, and 50% of the seed (S10/90, S30/70, S50/50), bulb (B10/90, B30/70, B50/50), and seed-bulb blend (SB10/90, SB30/70, SB50/50). Sixty male albino rats, divided into 10 groups of six rats each, were used. Group 1 (control) was fed rat chow only; groups 2-10 were fed the experimental diets. After 28 days of feeding, the animals were sacrificed and whole blood collected for full blood counts. The results, compared to the control (p<0.05), showed that supplementation exceeding S30/70 and B10/90 or SB10/90 showed significant (p<0.05) loss of body weight. Total WBC, lymphocyte and eosinophil counts were significantly (p<0.05) increased in the experimental groups. Hb was reduced in group B10/90 but RBC, Hb and PCV increased in B10/90 and SB10/90. The same trend was observed in MCV of groups B10/90 and B50/50. Platelet counts in the experimental groups were significantly similar to the control though lower in S30/70, B50/50 and SB50/50. Overall, data from this study suggest that diet supplementation with 10% jackfruit bulb or the seed-bulb blend is likely to provide some antianaemic benefits and enhance the immune system.
Haematinic evaluation of Lauha bhasma and Mandura bhasma on HgCl 2 -induced anemia in rats
Indian Journal of Pharmaceutical Sciences, 2007
The present study was carried out to evaluate the haematinic effect of two ayurvedic preparations of iron on mercuric chloride-induced anemia in rats. Lauha bhasma and Mandura bhasma, two well-known ayurvedic iron preparations, are commonly used to treat anemia. In Charles Foster strain rats of either sex, anemia was induced by administering mercuric chloride (9 mg/kg). Lauha bhasma and Mandura bhasma (11 mg/kg) were evaluated for their haematinic activity. The observed results suggest that Lauha bhasma and Mandura bhasma possess signifi cant (P<0.05) haematinic and cytoprotective activity.
Effect of Carica papaya Leaf Juice on Hematology of Mice (Mus musculus) with Anemia
Biosaintifika: Journal of Biology & Biology Education, 2017
Anemia is defined as a decrease in haemoglobin concentration and the number of erythrocytes. One source of iron in vegetable is the papaya leaf. This study aimed to determine the number of erythrocytes and hemoglobin levels after the application of Carica papaya leaf on mice through the sodium nitrite induction. The research design used was Randomized Design Complete by using five treatments with five repetitions on each treatment. The data measured in this study was number of erythrocytes and hemoglobin levels of rats before being given sodium nitrite, after being given sodium nitrite and after being given papaya leaf juice. The data obtained were analyzed statistically by using variance analysis (ANOVA), and the data that significantly different was then analyzed by Duncan Multiple Range Test (DMRT). The results showed that papaya leaf juice significantly affected the increase of erythrocyte number and hemoglobin level. The concentration that gives the best effect in increasing th...
Moringa oleifera Diets Effect on Haematological Parameters of Rat (Ratus norvegicus)
Journal of Biosciences and Medicines, 2020
With aim of place at the disposal of the laboratories nonconventional food with food values and which do not have impact on the system haematopoietic of the rats of laboratory in particular the rat of the species Rattus norvegicus, the study of the haematologic parameters of rats nourished with nonconventional food makes containing powder of Moringa oleifera was undertaken. Indeed, analysis of the blood taking away carried out following the administration of five diets container various rates of powder of Moringa oleifera (0% (Control food), 25%, 50%, 75% and 100%) respectively codified L3P, L3P25, L3P50, L3P75 and L3P100. These different food formulations were administered to rats (Ratus norvegicus) during three months. The weight of these animals was daily taken, followed by blood samples every 30 days for the determination of the blood count. The results of the study were shown differences between the various food formulations for each period of blood samples in biological parameters. These differences were more significant for platelets. During the three months of study, the hematocrit, platelets, MCV and outside the control group, were reported a slight progressive increase. The leukocyte parameters of all the rats in the investigation, indicated an increase in the second month. In conclusion, the study shows that during the three months of investigation, the tested foods do not disturb the haematological parameters except for thrombocytes which reveal a modification.
Hematological Indices of Experimental Rats Fed with Rat Chow Fortified with Processed Breadfruits
Asian Journal of Biochemistry, Genetics and Molecular Biology
Background: The abundance of nutrients, vitamins, and fiber in breadfruit has been documented in many literature studies that can hardly be exhausted and are consumed majorly by the Igbos and other southern parts of Nigeria to ameliorate hunger. Aim: This study however was aimed at determining the effect of feeds fortified with grilled, preboiled, and cooked breadfruit on hematological indices of wistar rats. Methods: Hematological parameters were determined using an automated hematology analyzer (Mindray-BC-5300). The parameters analyzed include Hemoglobin (HGB), Packed Cell Volume (PCV), Red Blood Cells (RBC), Platelets (PLT), Mean Corpuscular Volume (MCV), Mean Corpuscular Hemoglobin (MCH), Mean Corpuscular Hemoglobin Concentration (MCHC), White Blood Cells (WBC), Neutrophils (NEUT), Lymphocytes (LYMPH), Monocytes (MON), Eosinophils (EOS) and Basophils (BAS). Results: The result from the experiment indicated that all fortified groups showed no significant increase or decrease (p&...