Antitubercular activities of five Medicinal Plants against Two Strains of Mycobacterium tuberculosis (original) (raw)
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Potential of plant extracts against Mycobacterium tuberculosis: an integrative review
Peer Review, 2023
Mycobacterium tuberculosis (MTB) is the main cause of tuberculosis. For decades, new forms of treatment and new ways of dealing with the growing resistance acquired by this bacterium to first-line drugs have been studied. Medicinal plants are a source of new bioactive compounds with antimicrobial potential. Objective: to investigate which plant species have already been tested and which main secondary metabolites are active against MTB. Methodology: an integrative review that included in vitro experimental studies, carried out around the world, that used medicinal plant extracts to evaluate the antimycobacterial activity by microdilution, with identification of major compounds, against MTB, between 2011 and 2021, the Web of Science and PubMed were used and the descriptors "medicinal plants AND against AND MTB". Results: 20 species of plants with antimycobacterial activities were found. Four stood out with MIC<10 µg/mL. The variety of secondary metabolites was determinant for antimycobacterial activity, highlighting alkaloids, terpenes and phenolic compounds. Conclusion: the number of secondary metabolites obtained in the extraction is decisive in the antimycobacterial activity.
The Indian journal of medical research, 2010
Emergence of multi-drug resistant (MDR) and extensively-drug resistant (XDR) strains of Mycobacterium tuberculosis has further complicated the problem of tuberculosis (TB) control. Medicinal plants offer a hope for developing alternate medicines for the treatment of TB. The present study was done to evaluate in vitro anti-tubercular activity of five medicinal plants viz., Acalypha indica, Adhatoda vasica, Allium cepa, Allium sativum and Aloe vera. Aqueous extracts of leaves of A. indica, A. vasica, bulbs of A. cepa, cloves of A. sativum and pure gel of A. vera leaves, were tested in vitro for their activity against two MDR isolates (DKU-156 and JAL-1236), reference susceptible strain M. tuberculosis H37Rv as well as rapid grower mycobacterial pathogen M. fortuitum (TMC-1529) using Lowenstein Jensen (L-J) medium and colorimetric BacT/ ALERT 3D system. Activity in L-J medium was evaluated by percentage inhibition which was calculated by mean reduction in number of colonies on extract ...
In Vitro Antimycobacterial and Cytotoxic Data on Medicinal Plants Used to Treat Tuberculosis
Data in Brief, 2016
This article contains data on in vitro antimycobacterial activity and cytotoxicity of hydroethanolic crude extracts from five selected medicinal plant species traditionally used to treat tuberculosis in Ghanaian ethnomedicine, see "Medicinal plants used to treat TB in Ghana" [1]. The interpretation and discussion of these data and further extensive insights into drug discovery against tuberculosis from natural products of plant biodiversity can be found in "Antimycobacterial and cytotoxic activity of selected medicinal plant extracts" [2].
Agriculture and Biology Journal of North America, 2011
Tuberculosis (TB) continues to be a devastating disease worldwide in the 21 st Century and it is believed that one third of the World's populations are infected. 22 Countries are responsible for over 80% of the global TB burden, Nigeria is rank 4 th and have the highest burden in Africa. A number of anti tuberculosis drugs are ineffective against this disease due to the emergence of resistance strains. As part of the effort in developing a new anti tuberculosis agent, we decided to interact with traditional medicine practitioners of the Plateau region on their approach to the management of TB. A total of fourteen (14) plants representing nine families were identified as the most often used plants to manage TB symptoms in the region. The plants materials were extracted using 70% aq. methanol and the extracts obtained after concentration were screened against BCG. The results of the assay showed that 56% of the plants screened had MICs within the range of 800µg/ml to 2000µg/ml and as such had antimycobacterial activity. These plants are potential sources for herbal drugs for exhibiting these strong inhibitory properties and could also provide leads to tuberculosis drug development.
Phytochemical evaluation and anti-tuberculosis activity of selected nine ethnomedicinal plants
International Journal of Herbal Medicine, 2017
Tuberculosis is one of the leading infectious disease and health burden in the world. It has been estimated that, one third of world's population including 40% from India estimated to be infected with tuberculosis. As plants are the important sources of diverse range of bioactive principles. The revival of interests in plant derived drugs is mainly due to the current widespread belief that green medicine is safe and more dependable than expensive synthetic drugs, which have adverse side. In this study nine healthy plants with medicinally potential were collected and different extracts were prepare to evaluate its phytochemical study and anti-tubercular activity on Mycobacterium smegmatis using two different models. There are many similarities between Mycobacterium smegmatis and the much more virulent obligate pathogens that are Mycobacteria. On the basis of both the models, form nine selected plants three plants A. vasika, O. sanctum and A. galanga shows maximum anti-tuberculosis activity at the MIC 100 μg/ml, 250 μg/ml and 250 μg/ml respectively. The present study has revealed the importance of plant extracts to control Mycobacterial infections which are being a threat to human health and for the development of alternate, safe and effective medicines.
Background: Tuberculosis (TB) is a global burden with one -third of the world's population infected with the pathogen Mycobacterium tuberculosis complex and annually 1.4 million deaths occur due to the disease. This high incidence of infection and the increased rate of multi-drug resistant and extensively-drug resistant strains of the organism further complicated the problem of TB control and have called for an urgent need to develop new anti-TB drugs from plants. In this study, the in vitro activity of root of Calpurnia aurea, seeds of Ocimum basilicum, leaves of Artemisia abyssinica, Croton macrostachyus, and Eucalyptus camaldulensis were evaluated against M. tuberculosis and M. bovis strains.
2020
The increase of multidrug-resistant Mycobacterium tuberculosis (MDR-TB) demands the search for alternative antimycobacterial drugs. The aim of this study was to evaluate plants used in Indian traditional medicine to treat respiratory diseases for activity against MDR-TB. Six plants(Taxus baccata, Senna alata, Andrographis paniculata, Adhatoda vasica Nees, Acalypha indica L., Aloe vera L.) was selected for activity against Mycobacterium tuberculosis and aqueous extracts of these leaves at concentrations from 2%,4%,6% were tested in vitro for their activity against one MDR isolate (DKU-156), reference susceptible strain M. tuberculosis H37Rv as well as rapid grower mycobacterial pathogen M. fortuitum (TMC-1529) using Lowenstein Jensen (L-J) medium. Activity in L-J medium was evaluated by percentage inhibition which was calculated by mean reduction in number of colonies on extract containing as compared to extract free controls. Extracts of all the six plants Taxus baccata, Senna alata...
Inhibitory properties of selected South African medicinal plants against Mycobacterium tuberculosis
Journal of Ethnopharmacology, 2010
Ethnopharmacological relevance: Tuberculosis (TB), caused by Mycobacterium tuberculosis (MTB) is the most commonly notified disease and the fifth largest cause of mortality. One in 10 cases is resistant to treatment in some areas. Several plants are used locally to treat TB-related disease. Aims of the study: The aim was to screen selected South African medicinal plants used to treat TB and related symptoms by traditional healers for antimycobacterial activity. Materials and methods: Ethnobotanical information on these plants was obtained. Crude acetone, methanol, hexane and ethanol extracts of 21 selected medicinal plants obtained in Venda, South Africa were screened for their ability to inhibit MTB H 37 Ra and a clinical strain resistant to first-line drugs and one second-line drug using tetrazolium microplate assay to determine the minimum inhibitory concentration (MIC). Results were analyzed using Microsoft Excel 2007 and One way ANOVA; p < 0.05 was considered for statistical significance. Results: Few acetone extracts were active against MTB with MIC under 100 g/mL. Four plants showed lower MIC values; Berchemia discolor Klotzsch Hemsl 12, 5 g/mL on H 37 Ra and 10.5 g/mL on the clinical isolate, Bridelia micrantha Hochst. Baill (25 g/mL), Warbugia salutaris Bertol. F Chiov (25 g/mL), and Terminalia sericea Burch ex D. F (25 g/mL) on both H 37 Ra and clinical isolate. However, the roots of Ximenia caffra Sond. Var. caffra, barks of Sclerocarya birrea (A Rich) Hochst, Asclepias fruticosa L, tubers of Allium sativum L, leaves of Carica papaya L, Solanum panduriforme E. Mey C, and roots of Securidaca longepedunculata Fresen gave MIC greater than 100 g/mL. Conclusion: The acetone extracts of Berchemia discolor, Bridelia micrantha, Terminalia sericea and Warbugia salutaris could be important sources of mycobactericidal compounds against multidrug-resistant MTB.
An Overview on Anti-Tuberculosis Herbal Plants
2021
Mycobacterium tuberculosis (TB) is a highly infectious pathogen and causative agent for TB. There are many herbal extracts, which have been identified with potential claims of anti TB spectrum. Although established anti TB regimen with drugs are effective, these medications do cause certain adverse effects. The use of herbal extracts in treating TB is becoming more interesting and prevalent due to minimum adverse reactions observed with herbal medications. In this review, the various types, constituents and the Antitubercular activity of traditional herbal extracts have been discussed. Tuberculosis (TB) is a disease that has affected mankind from very ancient times. Anti-TB allopathic medications have been prescribed to control symptoms of this disease but results into side effects like hepatitis, hypersensitivity reactions, nausea, vomiting etc. The use of herbal medicine becoming popular due to toxicity and side effects of allopathic medicines. Medicinal plants from Ayurveda (Indi...
BMC Complementary and Alternative Medicine, 2013
Background: Tuberculosis (TB) is a global burden with one -third of the world's population infected with the pathogen Mycobacterium tuberculosis complex and annually 1.4 million deaths occur due to the disease. This high incidence of infection and the increased rate of multi-drug resistant and extensively-drug resistant strains of the organism further complicated the problem of TB control and have called for an urgent need to develop new anti-TB drugs from plants. In this study, the in vitro activity of root of Calpurnia aurea, seeds of Ocimum basilicum, leaves of Artemisia abyssinica, Croton macrostachyus, and Eucalyptus camaldulensis were evaluated against M. tuberculosis and M. bovis strains.