Analgesic and Anti-inflammatory Activity of Physalis angulata Linn. (Solanaceae) Leaf Methanolic Extract in Swiss Albino Mice (original) (raw)
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Anti-inflammatory, analgesic and antipyretic activities of Physalis minima Linn
Journal of Enzyme Inhibition and Medicinal Chemistry, 2009
In the present study, methanol extracts of Costus speciosus Koen. aerial parts were assessed for antiinflammatory, analgesic and antipyretic activities in experimental animals. The antiinflammatory activity of methanol extract of Costus speciosus (400 and 800 mg/kg, p.o.) was evaluated using carrageenan-induced paw oedema test. Analgesic effect was evaluated using acetic acid-induced writhing and Eddy's hot-plate models and antipyretic activity was assessed by Brewer's yeast-induced pyrexia in rats. The methanol extract of aerial parts of Costus speciosus in a dose of 400 and 800 mg/kg showed significant antiinflammatory activity (19.36 and 40.05% reduction) at 5 h postmedication. In analgesic models extract treated animals at (400 and 800 mg/kg) inhibited writhing's caused by acetic acid by 14.24 and 31.90%, respectively, and it also increased the latency period at both high and low doses which showed the mean reaction time at 16.60±0.355 s and 14.12±0.355 s, respectively, when compared to control in hot-plate test. It also reduces the rectal temperature of the animals at low and high doses significantly 37.03±0.108° and 36.63±0.098°, respectively, in Brewer's yeast induced pyrexia. The obtained results of the present investigation revealed that methanol extract of Costus speciosus has significant antiinflammatory, analgesic and antipyretic activities.
Antinociceptive effect of the aqueous extract obtained from roots of Physalis angulata L. on mice
Journal of …, 2006
In this study, we attempted to identify the possible antinociceptive action of aqueous extract (AE) obtained from roots of Physalis angulata, known in Brazil as "Camapu", used to treat various pain-related physiological conditions. The AE of Physalis angulata (10-30 mg/kg) given by i.p. or p.o. route, 0.5 and 1 h prior, produced significant inhibition of abdominal constrictions caused by acetic acid, with ID 50 values of 18.5 (17.4-19.8) and 21.5 (18.9-24.4) mg/kg and inhibitions of 83 ± 8 and 66 ± 5%, respectively. The AE (10-60 mg/kg, i.p.) also caused significant inhibition of the late-phase of formalin-induced pain, with an ID 50 value of 20.8 (18.4-23.4) mg/kg and inhibition of 100%. Treatment of mice with AE (60 mg/kg, i.p.) or with morphine (10 mg/kg, i.p.) produced a significant increase of the reaction time in the hot-plate test. These results demonstrate, for the first time, that the AE of Physalis angulata produce marked antinociception against the acetic acid-induced visceral pain and inflammatory pain responses induced by formalin in mice. The mechanism by which the AE produces antinociception still remains unclear. However, pharmacological and chemical studies are continuing in order to characterize the mechanism(s) responsible for the antinociceptive action and also to identify the active principles present in Physalis angulata. Moreover, the antinociceptive action demonstrated in the present study supports, at least partly, the ethnomedical uses of this plant. (J.L.M. Do Nascimento).
Physalis angulata extract exerts anti-inflammatory effects in rats by inhibiting different pathways
Journal of Ethnopharmacology, 2008
Physalis angulata is a popular medicine used in Brazil due to its anti-inflammatory effects, but the pharmacological mechanisms underlying these actions remain to be better understood. In the present work, lyophilized aqueous extract from the roots of Physalis angulata Linneu (AEPa) was used to control the inflammatory response induced by the injection of 1% carrageenan into subcutaneous rat's air pouches. Adenosine deaminase (ADA) activity, nitrite level, and prostaglandin E 2 (PGE 2 ) level were used to evaluate the action of inflammatory mediators. Tumor growth factor- (TGF-) level was used as a bioindicator of immunomodulatory response. Rats were injected with vehicle, indomethacin, or AEPa (0.5 mg/kg, 1 mg/kg, and 5 mg/kg i.p.), 1 h before carrageenan administration. AEPa at 0.5 mg/kg had no effect. However, 1 mg/kg of AEPa showed significant anti-inflammatory effects, decreasing exudate volume, total number of inflammatory cells, ADA activity, nitrite level, and PGE 2 level in 50%, 41%, 20%, 60%, and 41%, respectively. The anti-inflammatory effects of 5 mg/kg AEPa appeared to be more effective than those of 1 mg/kg AEPa (84%, 80%, 43%, 70%, and 75%, respectively). In addition, TGF- level was upregulated to 9700 pg/ml after 5 mg/kg AEPa, in comparison with 160 pg/ml in the vehicle-treated group, and 137 pg/ml in the indomethacin-treated group. The results indicate that AEPa exerts powerful anti-inflammatory and immunomodulatory activities, interfering with the cyclooxygenase pathway, lymphocyte proliferation, NO, and TGF- production.
The use of natural products by communities from the Colombian Caribbean region to treat health issues, together with biodiversity and geographical features, constitute a great scenery to develop new therapies based on ethnopharmacological heritage. Here, we investigated the anti-inflammatory potential of 10 commonly used plants in Colombian folk medicine, evaluating their effect on nitric oxide (NO) production by LPS-stimulated RAW 264.7 macrophages. The most active plant was evaluated in vivo using 12-O-tetradecanoyl-phorbol-13-acetate (TPA)-induced mouse ear edema, along with its effect on the production of pro-inflammatory mediators in vitro. The extract of Physalis angulata L. calyces showed the highest activity. This extract was fractionated and its dichloromethane fraction (DF) was the most active in vitro, inhibiting the production of NO, prostaglandin E 2 (PGE 2), interleukin (IL)-1b, IL-6, tumor necrosis factor (TNF)-a and monocyte chemotactic protein (MCP)-1 (CCL2). In vivo, DF showed a significant inhibition of ear edema and myeloperoxidase (MPO) activity, with evident reduction of the leukocyte infiltration into tissue. Our results support the ethnopharmacological use of the selected plants in folk medicine. P. angulata dichloromethane fraction represents a promising source of pharmacological compounds with great potential therapeutic use to treat inflammatory illness.
Aims: The aim of the present study was to investigate the analgesic and anti-inflammatory potential of the ethanolic extract of Phyllanthus acidus (Family: Phyllanthaceae) leaves on swiss albino mice. Study Design: The extract was divided into two concentrations (100 mg/kg and 200 mg/kg body weight) and was used for the examination of analgesic and anti-inflammatory activities on swiss albino mice. Methodology: The analgesic activity was evaluated using acetic acid induced writhing method, formalin induced paw licking method, tail immersion method and eddy's hot plate method. Carrageenan induced hind paw edema was performed to evaluate anti-inflammatory activity. Results: The extract exhibited significant (P < .001) inhibition of writhing at the dose 200 mg/kg body weight compared to control in acetic acid induced writhing method. In formalin induced paw Original Research Article Hossain et al.; EJMP, 13(1): 1-10, 2016; Article no.EJMP.24361 2 licking method, both the early and late phases significantly (P < .001) increased analgesia in a dose dependant manner. The extract at the dose of 200 mg/kg body weight was highly significant (P < .001) at 1h interval as compared to control in both tail immersion and eddy's hot plate method and inhibited 52.94% and 50% analgesia respectively. In anti-inflammatory activity test, the crude extract was highly significant (P < .001) and inhibited inflammation with time in a dose dependant manner. Conclusion: Our study reveals that P. acidus leaves extract possess significant analgesic and anti-inflammatory activity and the dose 200 mg/kg body weight is more significant than 100 mg/kg body weight in all of the methods.
Analgesic and anti-inflammatory properties of leaf extract
Journal of Ethnopharmacology, 2005
The aqueous and methanol extracts from the dry bulbils of Dioscorea bulbifera L. var sativa (Dioscoreaceae)-evaluated orally at the doses of 300 and 600 mg/kg against pain induced by acetic acid, formalin, pressure and against inflammation induced by carrageenan, histamine, serotonin and formalin in mice and rats, showed a dose dependant inhibition of pain and inflammation with a maximum effect of 56.38%, 73.06% and 42.79% produced by the aqueous extract, respectively on pain induced by acetic acid, formalin and pressure while the methanol extract at the same dose respectively inhibited these models of pain by 62.70%, 84.54% and 47.70%. The oral administration of aqueous and methanol extracts caused significant anti-inflammatory activity on paw oedema induced by histamine, serotonin and formalin. The present results show that the bulbils of Dioscorea bulbifera var sativa possess potent analgesic and anti-inflammatory activities. These activities may results from the inhibition of inflammatory mediators such as histamine, serotonin and prostaglandins. Thus, the analgesic activity of the bulbils of Dioscorea bulbifera may be at least partially linked to its anti-inflammatory activity.
African Journal of Traditional, Complementary and Alternative Medicines, 2008
The aqueous and methanol extracts from the dry bulbils of Dioscorea bulbifera L. var sativa (Dioscoreaceae)-evaluated orally at the doses of 300 and 600 mg/kg against pain induced by acetic acid, formalin, pressure and against inflammation induced by carrageenan, histamine, serotonin and formalin in mice and rats, showed a dose dependant inhibition of pain and inflammation with a maximum effect of 56.38%, 73.06% and 42.79% produced by the aqueous extract, respectively on pain induced by acetic acid, formalin and pressure while the methanol extract at the same dose respectively inhibited these models of pain by 62.70%, 84.54% and 47.70%. The oral administration of aqueous and methanol extracts caused significant anti-inflammatory activity on paw oedema induced by histamine, serotonin and formalin. The present results show that the bulbils of Dioscorea bulbifera var sativa possess potent analgesic and anti-inflammatory activities. These activities may results from the inhibition of inflammatory mediators such as histamine, serotonin and prostaglandins. Thus, the analgesic activity of the bulbils of Dioscorea bulbifera may be at least partially linked to its anti-inflammatory activity.
Antinociceptive Effect of Ethanol Extract of Physalis Angulata
International Journal Of Pharmaceutical And Bio-Medical Science
Pain is an unpleasant sensory and emotional experience associated with actual or potential tissue damage, or described in terms of such damage. Pain is the most common reason a patient sees a physician. However, conventional painkillers are not completely effective in controlling all pain syndromes; thus, additional efforts have been made to develop analgesic drugs from natural materials. In this study, the ethanolic extract of Physalis angulata (EEA) was examined for its antinociceptive activity at doses of 140, 280, and 560 mg/kg body weight. Acetic acid-induced writhing was used to evaluate the antinociceptive activity of EEPa. The results showed that the EEPa (140, 280, and 560 mg/kg BW) had significant antinociceptive activity. The percentage of inhibition was 29,41%, 37,96%, 44,38%, respectively. These findings suggest that Physalis angulata leaves extracts possess promising antinociceptive effects.
Antinociceptive Properties of Physalins from Physalis angulata
Journal of Natural Products, 2014
Pain is the most common reason a patient sees a physician. Nevertheless, the use of typical painkillers is not completely effective in controlling all pain syndromes; therefore further attempts have been made to develop improved analgesic drugs. The present study was undertaken to evaluate the antinociceptive properties of physalins B (1), D (2), F (3), and G (4) isolated from Physalis angulata in inflammatory and centrally mediated pain tests in mice. Systemic pretreatment with 1−4 produced dose-related antinociceptive effects on the writhing and formalin tests, traditional screening tools for the assessment of analgesic drugs. On the other hand, only 3 inhibited inflammatory parameters such as hyperalgesia, edema, and local production of TNF-α following induction with complete Freund's adjuvant. Treatment with 1, 3, and 4 produced an antinociceptive effect on the tail flick test, suggesting a centrally mediated antinociception. Reinforcing this idea, 2−4 enhanced the mice latency reaction time during the hot plate test. Mice treated with physalins did not demonstrate motor performance alterations. These results suggest that 1−4 present antinociceptive properties associated with central, but not anti-inflammatory, events and indicate a new pharmacological property of physalins.
2018
The petroleum ether, ethyl acetate, and methanol extracts of Phyllanthus reticulatus. Poir. (Euphorbiaceae) were chosen for pharmacological screening. Comparative antioxidant, antibacterial activities and general toxicity studies on the n-hexane, dichloromethane (DCM) and methanol (MeOH) extracts of Mimosa pudicaand Mimosa rubicaulis, two Bangladeshi medicinal plants, were carried out, using the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assay, the resazurin microtitre plate based assay, and the brine shrimp lethality assay, respectively. The research work was conducted to investigate the in vivo Analgesic and Anti-inflammatory effects of Leaves of Phyllanthus reticulatus and Mimosa pigra. The Leaves of both plants extracted with Methanol (80%). The Analgesic activity of the extracts was performed using acetic acid induced Writhing in mice and Formalin induced to have Analgesic activity of the dose 100 and 200mg/kg body weight in the tested models compared to control. The percent of inhi...