Rapid validated HPTLC method for estimation of piperine and piperlongumine in root of Piper longum extract and its commercial formulation (original) (raw)
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Quantification of Piperine from Single herb formulation containing Piper nigrum by HPTLC
2016
Background: Black pepper is the important plant in Ayurveda possessing Immunomodulator property. Objectives: To develop HPTLC method for Piperine from single herb formulation. Settings and Design: A HPTLC method was developed for the determination of Piperine in Single herb formulation. Materials Methods: Analysis of Piperine was performed on HPTLC aluminium plates pre-coated with silica gel 60F254 as a stationary phase. Ascending development was carried out in twin trough glass chamber previously saturated with mobile phase consisting of toluene: ethyl acetate: formic acid (8: 3: 0.5 v/v/v) at room temperature (25±2ºC). The densitometric scanning was carried out at wavelength 340 nm by using Camag TLC scanner-3. Statistical Analysis: The RSD and Correlation coefficient (r 2) were calculated by standard formulas Results: Piperine was appeared in chromatographic zones at Rf value 0.52 for Single herb formulation. The linear calibration plots showed good results with r 2 = 0.998. Conc...
Journal of Pharmacognosy and Phytochemistry, 2018
Herbal medicines widely used in health-care in both developed as well as developing nations. Herbals are usually considered harmless and ever supplementary being consumed by people devoid of prescription. Though, some of them leads to health troubles, some are not effective and some might be interact with supplementary drugs. Consequently, standardization of herbal formulations is essential in order to estimate the quality of drugs, based on the concentration of their active principles. In the present study, Shatavari based Galactogogue, herbal formulation manufactured by Fredun Healthcare Pvt. Ltd., Mumbai is used for standardization. The HPTLC method was validated according to ICH guidelines and shown to be specific, linear, repeatable and accurate, within the established ranges. Statistical analysis proves that the method is reproducible and selective. This method can be used for the quantitative determination of piperine in herbal extract and its formulations.
HPTLC method for analysis of piperine in fruits ofPiperspecies
Journal of Planar Chromatography – Modern TLC, 2011
Piperine, an alkaloid with diverse biological activity commonly occurring in fruits of Piper sp., has high commercial, economical, and medicinal value. In this communication densitometric estimation of piperine from fruits of Piper nigrum L., processed Piper nigrum L. (white pepper), Piper longum L., Piper retrofractum Vahl, Piper cubeba Hunter, and Piper betle L. is reported. Extracts of these fruits and a standard solution of piperine were applied to silica gel F 254 HPTLC plates and the plates were developed in a twin-trough chamber with toluene-ethyl acetate-diethyl ether 6:3:1 as mobile phase. The plates were scanned at 337 nm and quantification of piperine was based on a predetermined calibration plot obtained by chromatography of 15 to 75 ng standard. The quantity of piperine was highest in fruits of Piper nigrum L. and least in Piper betle L. The trend was Piper nigrum L. > Piper longum L. > processed Piper nigrum L. > Piper retrofractum Vahl > Piper cubeba Hunter > Piper betle L. The HPTLC method was found to enable simple, convenient, rapid screening and quantification of the active marker piperine in six fruits of Piper sp. commonly used in the Indian traditional system of medicine. 2 Experimental 2.1 Plant Material, Reagents, and Solutions Fruits of Piper nigrum (black pepper) and Piper longum, were collected from Dapoli, Maharashtra, India, and fruits of Piper betle were collected from Vir, Satara, Maharashtra, India during early 2008. Piper nigrum (white pepper), Piper retrofractum, and Piper cubeba were purchased from the crude drug market of Pune, Maharashtra, India. These fruits were authenticated and deposited in the crude drug repository of Agharkar Research Institute, Pune 411 004, India (voucher specimen numbers: Piper nigrum L.-F133, processed Piper nigrum L.-F132, Piper longum L.-F134, Piper retrofractum Vahl-F135, Piper cubeba Hunter-F136, and Piper betle L.-F137). Piperine standard was procured from Sigma-Aldrich (Steinheim, Germany; ≥98.0%). Other analytical grade solvents and reagents were purchased from SD Fine Chemicals, Mumbai, India. A stock solution of piperine (1 mg mL-1) was prepared by dissolving 10 mg, accurately weighted, in methanol and diluting to 10 mL. Stock solution (0.050 mL) was transferred to a volumetric flask and diluted to 10 mL with methanol to furnish working standard solution.
HPTLC method for analysis of piperine in fruits of Piper species
JPC - Journal of Planar Chromatography - Modern TLC, 2011
Piperine, an alkaloid with diverse biological activity commonly occurring in fruits of Piper sp., has high commercial, economical, and medicinal value. In this communication densitometric estimation of piperine from fruits of Piper nigrum L., processed Piper nigrum L. (white pepper), Piper longum L., Piper retrofractum Vahl, Piper cubeba Hunter, and Piper betle L. is reported. Extracts of these fruits and a standard solution of piperine were applied to silica gel F 254 HPTLC plates and the plates were developed in a twin-trough chamber with toluene-ethyl acetate-diethyl ether 6:3:1 as mobile phase. The plates were scanned at 337 nm and quantification of piperine was based on a predetermined calibration plot obtained by chromatography of 15 to 75 ng standard. The quantity of piperine was highest in fruits of Piper nigrum L. and least in Piper betle L. The trend was Piper nigrum L. > Piper longum L. > processed Piper nigrum L. > Piper retrofractum Vahl > Piper cubeba Hunter > Piper betle L. The HPTLC method was found to enable simple, convenient, rapid screening and quantification of the active marker piperine in six fruits of Piper sp. commonly used in the Indian traditional system of medicine.
Analysis for piperine in leaves, roots and spikes in Piper longum L
2018
Piper longum L (Pipali) is an important medicinal plant used as an analgesic, antipyretic, CNS depressant, anti-inflammatory, anti-tumor, sedative, hypnotic, tranquilizer and muscle relaxant, hepatoprotective both in digestive and respiratory systems. The fruit of Piper longum contains a large number of alkaloids and related compounds, among them the most important is piperine. Attempts were made to detect piperine in different parts of the Piper longum plant. Leaf and root of both male and female plants and spike from only female plant were used. Methanol extracts of above mentioned parts were prepared and subjected to thin layer chromatography (TLC) along with piperine as a standard, using toluene-ethyl acetate (70:30) mobile phase. Upon treatment with vanillin-sulphuric acid reagent and subsequent heating the silica gels and viewed under UV light. Appearance of yellow fluorescence was observed in all the samples except the male leaf. This confirmed the presence of piperine in all...
A rapid HPTLC method to estimate piperine in Ayurvedic formulations
Journal of Ayurveda and Integrative Medicine, 2019
Background: Trikatu, Sitopaladi, Hingavastaka, Avipattikara, Sringyadi and Talisadya are very popular Ayurvedic (churna) medicines practiced in India; however, unfortunately, they possess several quality control issues. Objective: The aim of this study was to find out a simple, accurate and sensitive HPTLC method for the detection and quantification of marker molecule, piperine (alkaloid) on these Ayurvedic formulations for standardization. Materials and methods: Methanolic extraction (reflux) was performed from the above six churnas as well as three single ingredients Piper longum (pipul), Piper nigrum (marich) and Piper chaba (chai). HPTLC was done using piperine as a standard. The mobile phase was a mixture of toluene-ethyl acetate (7:3, v/v) and detection at 342l. Results: The R f was detected at 0.39. Piperine was quantified in all samples. P. nigrum showed higher piperine than P. longum and P. chaba. The maximum piperine was noted in Hingavastaka churna and followed by Sringyadi churna, Sitopaladi churna, Talisadya churna, Trikatu churna and Avipattikara churna. Conclusion: This method can be successfully employed for standardization and quantitative analysis of piperine in Ayurvedic formulations (churnas) and also be helpful to clinicians and pharmacists to draw significant role of piperine present in all these samples.
2011
Piperine, a characterizing compound present in fruits of Piper nigrum and Piper longum used as bioavailability enhancer. Ingredients of antioxidant, anti-inflammatory activity has been extracted using soxhlet and supercritical fluid extraction technique. It was isolated using column chromatography. Characterization of compound was done by spectroscopic technique. A simple, rapid, accurate and specific HPTLC method developed and validated. The method proposed can be used for the routine analysis of both P.nigrum and P.longum fruit material and its formulations.
QUANTIFICATION OF PIPERINE BY HPTLC METHOD
In present study HPTLC method has been carried out for determination of piperine of different manufactures. These days' numbers of Pharmaceutical companies are manufacturing Tribhuvankirti Rasa. In this study five samples out of which one is lab prepared (self-prepared) Tribhuvankirti Rasa and four are market preparations. The alcoholic extract of Tribhuvankirti Rasa and standard piperine samples were applied on TLC aluminium plate pre coated with silica gel 60 F 254 and developed using toluene: Ethyl acetate (7: 3) v/v as a mobile phase, using UV detector at wavelength of 254 nm. Content of marker compound in the samples were found similar.
Pharmacology & Pharmacy
In the pharmaceutical world the majority of the active pharmaceutical ingredients (API) have been obtained from the natural products. Piperine is such naturally occurring alkaloid which can be considered as major bioactive phytochemical having broad spectrum of pharmacological activities. It is obtained from the most valuable ethnomedicinal spices peppercorns i.e. black pepper and white pepper, which are the fruits of the Asian vine Piper nigrum L. Because of the widespread traditional uses of this medicinal compound, present article reveals a simple and effective isolation method of bulk piperine. The novelty of this investigation is to provide an idea for utilizing such natural method of large scale commercial piperine production as API drug in spite of chemical synthesis. Piperine was isolated in a pure crystal form and characterized by its melting point, X-Ray diffraction (XRD) studies and spectral data, including two-dimensional nuclear magnetic resonance (2D-NMR) spectroscopy. Chromatographic techniques like Thin Layer Chromatography (TLC) and High Performance Liquid Chromatography (HPLC) were applied to determine the purity of the yielded piperine. It was found that piperine yield from black pepper was within 2.5%-3.0% and from white pepper within 4.0%-4.5% and the purity of the yielded piperine was found to be up to 98.5% for black pepper and 98.2% for white pepper. Considering this yield value and purity it is indicated that, such effective isolation method can be successfully utilized for industrial large-scale production commercially. According to the result, it can be claimed that, as a natural product the isolated piperine can also be utilized as API drug like other expensive chemically synthesized piperine in different drug formulation.
Pharmacology & Pharmacy, 2017
In the pharmaceutical world the majority of the active pharmaceutical ingredients (API) have been obtained from the natural products. Piperine is such naturally occurring alkaloid which can be considered as major bioactive phytochemical having broad spectrum of pharmacological activities. It is obtained from the most valuable ethnomedicinal spices peppercorns i.e. black pepper and white pepper, which are the fruits of the Asian vine Piper nigrum L. Because of the widespread traditional uses of this medicinal compound, present article reveals a simple and effective isolation method of bulk piperine. The novelty of this investigation is to provide an idea for utilizing such natural method of large scale commercial piperine production as API drug in spite of chemical synthesis. Piperine was isolated in a pure crystal form and characterized by its melting point, X-Ray diffraction (XRD) studies and spectral data, including two-dimensional nuclear magnetic resonance (2D-NMR) spectroscopy. Chromatographic techniques like Thin Layer Chromatography (TLC) and High Performance Liquid Chromatography (HPLC) were applied to determine the purity of the yielded piperine. It was found that piperine yield from black pepper was within 2.5%-3.0% and from white pepper within 4.0%-4.5% and the purity of the yielded piperine was found to be up to 98.5% for black pepper and 98.2% for white pepper. Considering this yield value and purity it is indicated that, such effective isolation method can be successfully utilized for industrial large-scale production commercially. According to the result, it can be claimed that, as a natural product the isolated piperine can also be utilized as API drug like other expensive chemically synthesized piperine in different drug formulation.