Characterization of Proteins from Sunflower Leaves and Seeds: Relationship of Biomass and Seed Yield (original) (raw)
Related papers
Characterization of sunflower seed and kernel proteins
Helia, 2010
Total sunflower proteins, storage proteins, and helianthinin (11S) and 2S albumin fractions and their respective subunits in seeds and kernels of three sunflower hybrids were analyzed. Protein contents were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and coupled with densitometry. The SDS-PAGE profiles of the seed and kernel proteins in the crude extracts for all genotypes showed a very similar number of protein bands (thirty two) in the electrophoretograms. Three polypeptide groups of helianthinin fraction were detected. Two of these were acidic (α, Mw = 36,800 -42,900 Da and α', Mw = 31,000 -35,300 Da), while one was basic (β, Mw=21,000 -29,600 Da). The molecular weight of the 2S albumin proteins ranged from 11,500 to 20,100 Da. According to our results, there were significant differences among the seed and kernel protein contents. The 2S albumin content was significantly higher in kernels than in whole seeds of sunflower hybrids (P<0.05). By contrast, the 11S helianthinin content was significantly higher in seeds (where it ranged from 61.75 to 67.70% of totally extracted proteins) than in kernels (varied from 57.36 to 61.51% of totally extracted proteins) of sunflower hybrids (P<0.05).
Pakistan Journal of Agricultural Research, 2014
total proteins, oil content and fatty acids composition. Among parental lines, CMS-64, CMS-53, CMS-H55-2-2-1 and CMS-53 were the best general combiners for seed total proteins, oil content, oleic and linoleic acid, respectively. Among males, C-206-R, SF-187R, RHA-295 and RHA-854 were the potential parents exhibiting desirable GCA for seed total proteins, oil content, oleic and linoleic acids, respectively. Among the F hybrids, 1
Genetika, 2017
The most important criterion for introducing new confectionary sunflower hybrids into production is high protein yield. In the breeding process it is important to identify traits which could be used as selection criteria for increased kernel protein content. Increase of kernel protein content results in increased protein yield. This research was conducted during three vegetation seasons on 22 NS high-protein two-line confectionary sunflower hybrids produced within the breeding program at IFVCNS, Novi Sad, Serbia. Strong and very strong correlations were found among the largest number of examined traits. Based on the analysis of simple correlation coefficients, strong negative correlation was determined between kernel protein content and kernel ratio (-0.516*). A weak negative interdependence was determined between head diameter, seed protein content, and kernel protein content. Positive but weak correlation was determined between kernel protein content and thickness of seed, length ...
LWT - Food Science and Technology, 2012
Sunflower protein concentrates with different content of phenolic compounds were produced at pilot plant scale from the by-product of oil manufacturing. The products obtained were characterized, and their physicochemical properties (i.e. surface hydrophobicity, thermal stability, and polypeptide composition) were evaluated at different storage conditions. All the procedures evaluated resulted in sunflower protein concentrates with high protein solubility (>60%) but with different chemical composition, color, and physicochemical properties. The products exhibited intense coloration and antioxidant properties due to their residual phenolic compounds content. The addition of an isoelectric precipitation step increased the protein content and the removal of phenolic compounds. The resulting concentrates exhibited high protein digestibility in vitro, even in the presence of phenolic compounds, and maintained high protein solubility for at least 6 months of storage. During this period, only structural changes in proteins were observed, as evidenced by their surface hydrophobicity. The results demonstrate that it is feasible to produce sunflower protein concentrates with high solubility on a pilot plant scale, using sunflower oilcake as starting material.
Analysis of globulin maturation in developing sunflower seeds
Biologia Plantarum, 1991
The synthesis of major storage globulin polypeptides has been examined in developing seeds of sunflower (Helianthus annuus L.). Analyses of total proteins and purified globulins, also called helianthinin, by gel electrophoresis and immunoelectrophoresis have shown that a burst of protein synthesis and accumulation occurs around 10 d after flowering. There is no mature globulin before that time and only small amounts of precursor forms can be detected. Thus, 10--12 d after flowering appears to be a transition period during which genetic information for the globulin becomes actively expressed. Immunoelectrophoresis has confirmed that globulin is the main storage protein, at seed maturation, accounting up to 70 % of total proteins per kernel. Pulse chase experiments have shown that synthesis initially involves the formation of high molecular mass precursors and that storage proteins are post-translationaUy processed. Intermediary products, with molecular mass higher than early translational products, can be detected, together with mature globulin polypeptides.
Characterization of Sunflower Seed Proteins by Electrophoretic Techniques
2000
The seed proteins of sunflower (Helianthus annuus) were qualitatively and quantitatively investigated. Qualitative studies were carried out using different electrophoretic techniques (SDS-PAGE, Poro-PAGE, 2-D SOS-PAGE, lsoelectric focusing, Mapping gels). Analysis of the water extracted flour on SOS-PAGE and SDS-Poro-PAGE gave five major polypeptides with MWs of 63.5 KD, 60 KO, 58 KD, 55 KO and 51 KO. The pattern of
Proteomic Analysis of Near-Isogenic Sunflower Varieties Differing in Seed Oil Traits
Journal of Proteome Research, 2007
Near-isogenic sunflower lines containing 25% (inbred RHA280) and 48% (RHA801) oil by seed dry mass were comparatively analyzed in biological triplicate at 18 days after flowering using two-dimensional (both pI 3-10 and 4-7) Difference Gel Electrophoresis. Additionally, two inbred lines varying in oleic acid content, HA89 (18% oleic) and HA341 (89% oleic), were also analyzed in the same manner. Statistical analyses of these sunflower lines was performed beginning with fitting a mixed effects linear model to the log-transformed optical volume of each spot to account for gel variation, followed by testing the significance between varieties for mean transformed optical spot volumes. The p-values from the spot analysis procedures were then used to find the cutoff point for differential expression using a 10% false-discovery rate (FDR). Comparison of the oil content and oleic acid composition lines revealed 77 and 42 protein spots below the 10% FDR cutoff, respectively, and were therefore declared differentially expressed. Liquid chromatography-tandem mass spectrometry analysis of each of these protein spots resulted in assignments for 44 and 17 spots, respectively. Fructokinase, plastid phosphoglycerate kinase, and enolase proteins were determined to be up-regulated in the high oil line, while phosphofructokinase, cytosolic phosphoglucomutase, and cytsolic phosphoglycerate kinase were up-regulated in the low oil variety. Additionally, four activities involved in amino acid synthesis were up-regulated in the low oil variety in addition to 12S storage proteins and a protein similar to legumin storage protein. Interestingly, two 2-DE spots identified as 14-3-3 proteins were found to be up-regulated in high oleic acid variety. Alteration of glycolytic and amino acid biosynthetic enzymes, as well as storage protein levels, suggests seed oil content is tightly linked to carbohydrate metabolism and protein synthesis in a complex manner.
AgriScientia
Argentina is the world's leading exporter of confectionery sunflower. This work characterized the morphometric and nutritional composition of sunflower seeds from new confectionery genotypes. Average morphometric variables per seed were: weight 0.11 and 0.06 g with and without achene, respectively; length 15.2 mm; and width 8.50 mm, with most calibers greater than 9.51 mm. Proximate analysis indicated that seeds without shell were an important source of lipids (49 %), as well as proteins (28 %), carbohydrates (11 %), and minerals (4.8 % ashes). Fatty acid composition showed that polyunsaturated fatty acids were the major components (56 %), followed by monounsaturated ones (34 %), and saturated ones (11 %). The mid-oleic genotype developed by INTA showed significantly greater oleic acid (47 %) and lower linoleic acid (42 %) than the commercial hybrid. Proteic composition showed 33 % of essential amino acids (EAA) while fiber and sugars represented 3.9 % and 7.3 % respectively; ma...