Trial of experimental toothpastes regarding quality for cleaning dentures (original) (raw)
Related papers
The International journal of prosthodontics, 2021
PURPOSE To evaluate the effects of 0.2% sodium hypochlorite, Efferdent (Prestige Consumer Healthcare), and 6.25% Ricinus communis on biofilm removal and antimicrobial action on dentures and brushes using nonimmersion or immersion protocols for the brushes. MATERIALS AND METHODS A total of 45 denture wearers were randomly assigned to a denture immersion protocol for 7 days: 0.85% saline solution for 20 minutes (control); 0.2% sodium hypochlorite for 20 minutes (SH); Efferdent for 3 minutes; or 6.25% Ricinus communis for 20 minutes (RC). The participants were also randomized to immersion (n = 23) or no immersion (n = 22) of their brushes with their dentures in the same solutions. For biofilm evaluation, the dentures were stained and photographed, and the area of the biofilm was measured using Image Tool 3.0 (University of Texas Health Science Center). To evaluate microbial load on dentures and brushes, the biofilm was collected, and the Candida spp and Streptococcus mutans colonies we...
A Comparative Analysis of the Effect of two Mouthrinses on the Accumulation of Biofilm on Dentures
IOSR Journal of Dental and Medical Sciences, 2016
Introduction: One of the common problems among denture wearers is the formation of plaque on the surfaces of the dentures. For many patients mechanical denture hygiene measure may be insufficient to achieve plaque removal on the dentures hence the rationale for the use of mouth rinses. Thus, this study aimed at comparing the effect of two mouth rinses on the accumulation of biofilm on dentures. Methodology: Patients were selected through a simple random sampling method (balloting) and subsequently assigned into 3 study groups; chlorhexidine digluconate group(CHX), hexetidine group and control group (normal tap water) with each group consisting of 43 patients. All the patients attended thrice: day 1 (baseline), day 7 (1 st recall) and day 14 (2 nd recall). Stained surface analysis was done using Image processing software 2012 (Image J tool 3.0 for Microsoft windows). Further descriptive and inferential analysis was done using IBM SPSS version 20.0 and test of statistical significance was done using Analysis of variance (ANOVA). The confidence level was set at 95% and the P-value of 0.05 or less was taken as statistically significant. Result: Mean percentage biofilm coverage area for CHX group was 31.43%, hexetidine group was 29.58% while that of control group was 54.55%. The ANOVA result showed that the mean difference between percentage biofilm coverage area of CHX and hexetidine groups was not statistically significant (P=0.215), whilst the difference between the experimental groups (CHX and hexetidine) and the control group was statistically significant (P=0.001) Conclusion: The tested denture cleansing agents used in this study, CHX and hexetidine solutions were equally efficacious in reducing biofilm and were superior to the control agent (water).
Efficacy of three denture brushes on biofilm removal from complete dentures
Journal of Applied Oral Science, 2007
he aim of this study was to compare the efficacy of three denture brushes (Bitufo-B; Medic Denture-MD; Colgate-C) on biofilm removal from upper and lower dentures using a specific dentifrice (Corega Brite). The correlation between biofilm levels on the internal and external surfaces of the upper and lower dentures was also evaluated. A microbiological assay was performed to assess the growth of colony-formed units (cfu) of Candida yeasts on denture surface. Thirty-three patients were enrolled in a 10-week trial divided in two stages: 1 (control)-three daily water rinses within 1 week; 2-three daily brushings within 3 weeks per tested brush. Internal (tissue) and external (right buccal flange) surfaces of the complete dentures were disclosed (neutral red 1%) and photographed. Total denture areas and disclosed biofilm areas were measured using Image Tool 3.00 software for biofilm quantification. Dentures were boxed with #7 wax and culture medium (CHROMagar TM Candida) was poured to reproduce the internal surface. Statistical analysis by Friedman's test showed significant difference (p<0.01) between control and brushing stages. No difference was found among the brushes with respect to their efficacy on biofilm removal (p>0.01). Analysis by the Correlation test showed higher r values (B=0.78; MD=0.8341, C=0.7362) for the lower dentures comparing the surfaces (internal and external) and higher r values (B=0.7861, MD=0.7955, C=0.8298) for the external surface comparing the dentures (upper and lower). The results of the microbiological showed no significant difference (p>0.01) between the brushes with respect to the frequency of the species of yeasts (chi-square test). In conclusion, all denture brushes evaluated in this study were effective in the removal of biofilm. There was better correlation of biofilm levels between the surfaces for the lower dentures, and between the dentures for the external surface. There was no significant difference among the brushes regarding the frequency of yeasts.
Comparative Analysis of Different Chemical Methods for Removing Biofilm from Complete Dentures
Journal of Dentistry, Oral Disorders & Therapy
The aim of this study was to make a comparative evaluation of three chemical substances for cleaning complete dentures, as regards their efficacy of biofilm removal. The sample consisted of 20 maxillary complete dentures. The dentures were randomly divided into groups according to the chemical cleaning method to be used: Group 1-water (control), Group 2-sodium hypochlorite solution, Group 3-sodium perborate (Corega Tabs®) and Group 4-2% chlorhexidine. The groups were evaluated in terms of the quantity of biofilm before and after application of the chemical cleaning method by applying a revealer, and later, analysed by the Denture Hygiene Index (DHI). The results showed that only sodium hypochlorite solution was effective for biofilm removal. There was statistically significant difference among the groups, pointing out greater efficacy of the method used in Group 2 in comparison with Groups 1 and 4. Group 3 did not differ statistically from any other group evaluated. The results allowed to conclude that sodium hypochlorite solution is the most efficient chemical agent for removing biofilm from complete dentures. However, when used alone, all the tested chemical cleaning methods were incapable of eliminating all the biofilm from denture surfaces.
Brazilian Dental Journal, 2013
This study compared the levels of biofilm in maxillary and mandibular complete dentures and evaluated the number of colony-forming units (cfu) of yeasts, after using auxiliary brushing agents and artificial saliva. Twenty-three denture wearers with hyposalivation and xerostomia were instructed to brush the dentures 3 times a day during 3 weeks with the following products: Corega Brite denture dentifrice, neutral liquid soap, Corega Brite combined with Oral Balance (artificial saliva) or tap water. For biofilm quantification, the internal surfaces of the dentures were disclosed, photographed and measured using a software. For microbiological analysis, the biofilm was scrapped off, and the harvested material was diluted, sown in CHROMagar™ Candida and incubated at 37°C for 48 h. Data were analyzed statistically by two-way ANOVA and Tukey's test (α=0.05). Mandibular dentures presented a mean biofilm percentage (µ=26.90 ± 21.10) significantly greater than the maxillary ones (µ=18.0 ...
2004
The efficacy of 2 oral hygiene products, an experimental toothpaste specific for complete denture cleansing and a regular standard toothpaste, was compared in terms of denture biofilm removal and cure of palatal lesions in patients with atrophic chronic candidiasis. The degree of correlation between presence of biofilm and mucosa erythema was also evaluated. Twenty-four complete denture wearers (45-80 years old) were divided into 2 groups: experimental paste and standard toothpaste (Sorriso-Kolynos, Brazil). Both groups received soft toothbrushes. The internal surfaces of upper dentures were stained using 1% sodium fluorescein and photographed at a 45° angle at 0, 15, 30 and 60 days. The slides were scanned and the areas of interest (denture total area and biofilm area) were measured (Image Tool software). The degree of erythema was evaluated on slides according to the Prosthesis Tissue Index. There was a significant reduction (1%) in the degree of biofilm (ANOVA/Tukey) between the two initial visits (0 and 15 days) and the two final visits (30 and 60 days), and in the average erythema scores (Kruskal-Wallis) between 0 and 60 days, in both groups. The Mann-Whitney test showed a significant difference (1%) between pastes in terms of biofilm degree, but no difference was found for the erythema score. Correlation values between biofilm and erythema degree were 0.3801 (experimental paste) and (0.3678 (standard toothpaste). We may therefore conclude that the experimental product was efficient for the removal of denture plaque biofilm.
BMC Oral Health
Background The continuously increasing demand for removable denture appliances and the importance of adequate denture cleaning have led to the development of various denture cleansing products. The aim of this study was to evaluate the efficacy of two novel denture cleansing agents (GE and TM) and three commonly available cleansers (0.5% sodium hypochlorite; NaClO, 0.12% chlorhexidine gluconate; CHX, and Polident®; POL) on multispecies microbial biofilm formation, stain removal and physical properties of dentures. Methods The antimicrobial activities of denture cleansing agents were determined against major oral opportunistic pathogens including Streptococcus mutans, Staphylococcus aureus, Escherichia coli and Candida albicans, using time-kill assays. Multispecies microbial biofilms grown on acrylic resins for 72 h were generated to determine the antibiofilm effects of cleansing agents by confocal laser scanning microscopy (CLSM). Evaluations of the tea and coffee stain removal prop...
In vitro and clinical evaluation of specific dentifrices for complete denture hygiene
Gerodontology, 2009
Objectives: To study the physical properties of two experimental dentifrices for complete denture hygiene, their effect on denture biofilm removal and antimicrobial properties by means of a clinical trial.Materials and methods: The experimental dentifrices comprised two compositions. One was based on the addition of 1% chloramine T (D1) and the other on the presence of 0.01% fluorosurfactant (D2). Measurements of density, pH, consistency, rheological features and abrasiveness were conducted. Sixty complete denture wearers were randomly assigned to three groups and were instructed to brush their dentures with a specific toothbrush: (1) Water (control); (2) D1; or (3) D2. Each method was used for 21 days. Denture biofilm was disclosed by a 1% neutral red solution and quantified by means of digital photos taken from the internal surface. Microbiological assessment was conducted to quantify Candida sp. and mutans streptococci. Data were evaluated by one-way anova and Tukey HSD, or Kruskal–Wallis (α = 0.05).Results: Both dentifrices decreased biofilm coverage when compared with the control group. D1 was the most efficacious treatment to reduce mutans streptococci, whereas D2 showed an intermediate outcome (anova, p < 0.040). No treatment influenced Candida albicans or non-albicans species (Kruskal–Wallis, p = 0.163 and 0.746, respectively).Conclusion: It can be concluded that brushing complete dentures with the experimental dentifrices tested could be effective for the removal of denture biofilm.
Evaluation of Toothpaste Effectiveness in Reducing Oral Bacteria
2019
This in vitro study was undertaken to determine which toothpaste represses oral bacteria ( Streptococcus mutans and Lactobacillus species) growth most effectively. The toothpaste samples used were nine-fluoride-containing toothpastes (A, B, C, D, F, G, H, I and J) and one non-fluoride containing toothpaste (E). Disc diffusion tests were performed to examine the mean inhibition zones for each toothpaste/bacteria sample using 0.5g/ml and 0.4g/ml concentrated toothpaste slurries. Toothpaste samples B and D showed the highest mean zone of inhibition on exposure to S . mutans and Lactobacillus sp. respectively while the least inhibition zone and no observable zones were exhibited by toothpaste sample I. The challenge test done to determine their behavioural profile at set time intervals of 0.5, 2, 5, 10 and 15 minutes revealed the dentifrices to be intensively bactericidal on Streptococcus mutans while a fluctuative antibacterial activity was obtained with respect to Lactobacillus specie...
PLOS ONE, 2016
Background Appropriate oral hygiene is required to maintain oral health in denture wearers. This study aims to compare the role of denture cleaning methods in combination with overnight storage conditions on biofilm mass and composition on acrylic removable dentures. Methods In a cross-over randomized controlled trial in 13 older people, 4 conditions with 2 different mechanical cleaning methods and 2 overnight storage conditions were considered: (i) brushing and immersion in water without a cleansing tablet, (ii) brushing and immersion in water with a cleansing tablet, (iii) ultrasonic cleaning and immersion in water without a cleansing tablet, and (iv) ultrasonic cleaning and immersion in water with a cleansing tablet. Each test condition was performed for 5 consecutive days, preceded by a 2-days wash-out period. Biofilm samples were taken at baseline (control) and at the end of each test period from a standardized region. Total and individual levels of selected oral bacteria (n = 20), and of Candida albicans were identified using the Polymerase Chain Reaction (PCR) technique. Denture biofilm coverage was scored using an analogue denture plaque score. Paired t-tests and Wilcoxon-signed rank tests were used to compare the test conditions. The level of significance was set at α< 5%. Results Overnight denture storage in water with a cleansing tablet significantly reduced the total bacterial count (p<0.01). The difference in total bacterial level between the two mechanical cleaning methods was not statistically significant. No significant effect was observed on the amount of Candida albicans nor on the analogue plaque scores.