Effect of Moringa oleifera Lam. Ethanol Leaf Extract on Hematology in Phenylhydrazine-induced Anemic Albino Wistar Rats (original) (raw)
Related papers
Journal of Medical and Biological Science Research, 2020
The leaves of Moringa oleifera have exceptional nutritional qualities and are used in traditional medicine to treat several diseases including anemia. This study is to propose a formulation for the optimal use of M. oleifera leaf powder in the treatment of anemia in rats. Phenylhydrazine (40 mg/kg) was administered intraperitoneally for 2 days to induce anemia in rats (Rattus norvegicus). The animals were divided into six groups of 6 rats each and treated orally from day D2 to D28. Rats groups 1, 2, 3 and 4 were treated orally, respectively with 400, 800 and 1600 mg/kg of M. oleifera aqueous extract of leaves (AEMo) and Ranferon ®. Groups 5 and 6 were fed ad libitum with diets containing 50% (P50) and 100% (P100) of M. oleifera leaves as a substitute for soybean meal, respectively. Red blood cell (RBC) parameters such as RBCs, hemoglobin (Hb), hematocrit (Ht), mean corpuscular volume (MCV) and mean corpuscular hemoglobin concentration (MCHC) were analyzed on days D0, D2, D5, D14 and D28. Results showed a prevalence of 0% in anemic rats treated with P50, AEMo 1600 mg/kg and Ranferon ® versus 16.67% in those treated with P100, AEMo 400 and 800 mg/kg at D28. In addition, P50, AEMo 1600 mg/kg and Ranferon ® significantly (p ˂ 0.001) increased RBCs, Hb, Ht with total recovery especially RBCs and Ht in anemic rats treated with P50 and Ranferon ®. In conclusion, the incorporation in small quantities of M. oleifera leaf powder as a substitute for soybean meal has a better efficacy on anemia than the total leaf extract of the same plant.
Corresponding author. e-mail: Hisham1212ribat@yahoo.com. This study is aimed at finding out the effect of Moringa oleifera on blood parameters and body weights of albino rats (n= 24) and rabbits (n= 10). The rats were divided into four groups; a control group and three experimental groups, while the rabbits were divided into two groups; a control group and an experimental one. The three experimental groups of rats were provided consecutively with100, 200 and 300 mg M. oleifera leave extract/kg of body weight daily for 21 days, while the experimental group of rabbits with 2.5 g fresh leaves of M. oleifera/Kg of body weight which was added to their feed daily for 21 days, and the control groups were fed on their diets without M. oleifera. The results showed significant differences (P < 0.05) in mean cell hemoglobin concentration (MCHC) and platelets (PLT) in the third group of rats (AL 3) and red blood cells (RBCs) count, hemoglobin (Hb) and MCHC in the fourth group (AL 4) while no...
EAS Journal of Pharmacy and Pharmacology, 2020
The present study was to evaluate the anti-anemic power of total aqueous extract of Moringa oleifera leaves (TAEMo) on phenylhydrazine induced anemic Wistar rats. First, hemolytic anemia was induced in rats by intraperitoneal administration of phenylhydrazine (PHZ) at the dose of 40 mg/kg body weight for two consecutive days. Then, the anemic rats were orally treated daily for 26 days with aqueous extract of Moringa oleifera leaves (TAEMo) at doses of 400, 800 and 1600 mg/kg b w. and Ranferon ®-12(Reference substance). The rats were analyzed for erythrocyte parameters such as erythrocyte cells, hemoglobin, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration on day 0, 2, 5, 14 and 28. The results revealed that the aqueous extract restored rapidly the modified erythrocyte parameters at the end of treatment. TAEMo at 1600 mg/kg showed an effect similar to the reference substance, Ranferon®-12. The study of reversibility after stopping treatment showed that not all erythrocyte parameters studied were significantly affected. This study found anti-anemic properties of Moringa oleifera leaves aqueous extract thus confirming the use of this plant in the treatment of anemia in the population.
African Journal Biomedical Research
The haematology and serum biochemical effects of oral administration of the ethanolic extract of the root of Moringa oleifera at 50, 100 and 150 mg/kg were investigated in 30 mated female Wistar rats. The rats were assigned into five groups of six rats each. Group A was given 50mg/kg of the extract; group B, 100mg/kg; group C, 150 mg/kg; group D, 0.2ml of corn oil; and group E, 0.2ml of distilled water. Groups D and E, corn oil and distilled water treatment groups respectively, served as the controls while groups A, B and C were the treatment groups. There was no significant difference (P>0.05) between the two control groups and the treatment groups for the RBC, WBC, PCV, MCV, MCH and MCHC and Hb. However, the mean lymphocyte values for groups B and C were significantly different (P<0.05) from those of group A as well the two control groups. The total protein, albumin, globulin and A/G ratio showed no significant difference (P>0.05) between the two control groups and the treatment groups. There were no significant differences (P>0.05) in the values of AST, ALP, creatinine, urea, GGT, glucose, cholesterol and ALT between the treatment groups and the control groups. It can therefore be concluded that oral administration of ethanolic extract of the root of M. oleifera is harmless to the rats since no adverse effects were detected in haematological and serum biochemical investigations .
Anti-Anemia Effect of Standardized Extract of Moringa Oleifera Lamk. Leaves on Aniline Induced Rats
Pharmacognosy Journal, 2016
Background: The leaves of Moringa (Moringa oleifera Lamk.) have been empirically used as anti-anemia for pregnant woman. Objectives: In the present study, the effect of ethanolic extract of moringa leaves was evaluated on the hematology parameters (hemoglobin, red blood cell count, hematocrit), on aniline induced white female rats. Materials and Methods: Thirty female rats strain Sprague-Dawley were divided into six groups: normal, anemia and ferrous fumarate group, and three groups of Moringa leaves extract. All groups, except normal group, induced by aniline at dose 0.005 ml/ g bw intraperitonially. On fifth day, normal and anemia groups were given 0.5% CMC, ferrous fumarate group was given ferrous fumarate orally and three other groups were given Moringa leaves extract at doses 198 mg, 396 mg, and 792 mg/200 g bw until the eleventh day. Results: The results showed that as Moringa leaves extracts increased the level of hemoglobin, red blood cell count, hematocrit, and total iron content in blood. Moreover, Moringa leaves extract at dose 792 mg/200 g bw can improve red blood cell's morphology and increased the level of hemoglobin and red blood cell count significantly (p<0.05). Conclusion: The ethanolic extract of Moringa leaves showed anti-anemia on aniline-induced rats.
Evaluation of the effect of ethanolic leaves extract of Maringa oleifera on Haematological and certain Biochemical parameters was studied. The study assessed Total protein, Albumin, Billirubin for biochemical and PCV, Hb and WBC for haematological indices. Twenty five (25) albino rats of wistar strain (100-175g) were used for the studies and were divided into 5 groups of five (5) rats per each. Group A served as control and was treated with distil water of treatment equivalence, group B, C,D, and E were treated with various concentration the extract as follows; viz: 100mg/kg, 200mg/kg, 300mg/kg, 1g/kg respectively. The administration of the extract lasted for twenty eight (28) days period after which the animals were sacrificed and blood obtained for biochemical and haematological analysis. The result of the study reveals that the ethanolic leaf extract of M. Oleifera lowers seruim bilirubin, but increase albumin, and Total protein levels in all treated groups compared with the controls all at (p<0.05). More so, similar increased was also recorded for Haemoglobin (Hb) and Hematocrit but no significant (p<0.05) changes was observed for WBC in all treatment groups. The leaves extract of M. oleifera administered orally prove to be biochemically and haematologically save.
Evaluation of Moringa Oleifera Lam Leaves (Moringaceae) Diets Against Induced Anemia in Wistar Rats
EAS Journal of Nutrition and Food Sciences, 2020
The effect of oleifera leaves diets against anemia was investigated in Wistar rats. Three groups of six rats each were formed. Moringa Hemolytic anemia was induced in rats by phenylhydrazine at a dose of 40 mg/kg body weight intraperitoneally for two successive days. The rats, which received phenylhydrazine were subjected to Ranferon® (Reference substance) and two régimes food P50% and P100% contained respectively 50% and 100% of sheet Moringa oleifera from day 2 (D2) until the end of the experiment to day 28 (D28). Blood was taken from all the rats to days D0, D2, D5, D14 and D28. Diets made from leaves of Moringa oleifera (P50% and P100%) and Ranferon® have significantly restored the red blood cell parameters changed by phenylhydrazine. In addition, P50% provided better values of erythrocyte parameters than P100%. However, no significant change in body weight was observed. This study revealed that these diets based on Moringa oleifera leaves has excellent therapeutic efficacy, thus confirming the use of this dietary supplement in the treatment of anemia.
Effect of Moringa oleifera aqueous leaf extract on some haematological indices in Wistar rats
The use of Moringa oleifera leaf extract in the treatment of virtually all ailments calls for further research to support the claim for its ability to boost blood. Thirty six albino rats of the Wistar strain weighing 200-230g were sorted into groups according to their weights and sex. The animals were divided into two groups, male and female. Three (3) test groups (1%, 5%, and 10% w/w) for the males containing five (5) animals each and a control group, for the females, three (3) test groups (1%, 5%, and 10% w/w) and control group with four (4) animals each. The result of haematological indices in female Wistar rats administered aqueous extract of M. oleifera shows that Hb count generally increased significantly in all the groups compared with their control group. Similarly, PCV and WBC generally increased in all the groups, this increase was significant for WBC in all the groups. RBC values showed a non-significant (p>0.05) value at 1% treated group but a significant increases (p...
This study evaluated the effectiveness of dry Moringa oleifera leaf powder in the management of anaemia in adult albino rats. The proximate, mineral, vitamin and phytochemical composition of dry M. oleifera leaf powder were analysed. Twelve adult albino rats grouped into three were used. Cyclophosphamide was used to induce anaemia into them. The percentage proximate values were protein (26.28%), ash (7.69%), carbohydrate (49.35%), crude fibre (7.48%) and moisture (7.05%). The rats whose feed were supplemented with M. oleifera leaf powder showed superior attributes to the unsupplemented group. The study showed dry M. oleifera leaf powder is promising in the management of anaemia.
Biomedical Journal of Indonesia: Jurnal Biomedik Fakultas Kedokteran Universitas Sriwijaya, 2020
The iron deficiency anemia is kind of anemia because the less of iron that make the function to tie oxygen is down. Young girl is one of sufferer group who's prone to get anemia. The long consequence of anemia for the young girl will disturb fatal function of some organ in their body. In general arise of headache symptoms and pain in the centre of head. And the in pregnancy will increase some complication risk such as maternal death. Premature born and perinatal death. One of nature ingredients (herbal) which is reported to have high iron content is kelor leaf (Moringa oleifera L) for about 28, 29 mg/100 g kelor. The purpose of this research is to know the effectiveness of Kelor leave fraction ((Moringa oleifera L) to increase hemoglobin grade with preclinical. Experimental research with complete random design (RAL) has been done in February till April 2019 in medical faculty laboratory of universitas sriwijaya Palembang. Sample of the research is female rat that has inclusion criteria as much 30 rats. Divided into 5 group. That is negative control group. Positive control (ferrous fumarate 60%), n-heksan fraction, ethyl acetate, and 1% concentration of kelor leave ethanol, the data of Hb all of subject before and after treatment from subject of iron deficiency anemia has collected by using Easy Touch GCHb tool. The analysis data of this research used SPSS 2.2 version. The result of the research said that the hemoglobin grade before and after treatment showed significant increasing(p<0,05) for n-hexane fraction and acetate ethyl >45%. While ethanol fraction only 32%.