Evaluation of Isotopic Abundance Ratio and Structural Properties of Magnesium Gluconate After Treatment with the Energy of Consciousness Using LC-MS and NMR Spectroscopy (original) (raw)
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Structural and Natural Isotopic Abundance Analysis of Magnesium Gluconate Treated with Energy of Consciousness (The Trivedi Effect®) Using LC-MS and NMR, 2017
The current research article was aimed to investigate the impact of The Trivedi Effect®- Energy of Consciousness Healing Treatment on the structural properties and isotopic abundance ratio (PM+1/PM and PM+2/PM) of magnesium gluconate using LC-MS and NMR spectroscopy. Magnesium gluconate was divided into two parts – one part was control, and another part was treated with The Trivedi Effect® remotely by eighteen renowned Biofield Energy Healers and defined as the Trivedi Effect® Treated sample. The liquid chromatogram of the control sample showed two peaks at Rt of 1.81 and 2.06 min, whereas the Trivedi Effect® Treated sample displayed these peaks at Rt of 1.79 and 2.03 min. The ESI-MS spectra of the control and the Trivedi Effect® Treated samples revealed the presence of the mass for magnesium gluconate ion in two forms at m/z 447 (adduct form with methanol) and 415 (protonated ion) in positive ionization mode. But, it showed the mass for the gluconate ion at m/z 195 in the negative ionization mode. The fragmentation pattern of magnesium gluconate in the treated sample was notably altered as compared to the control sample. The proton and carbon signals for CH, CH2 and CO groups in the proton and carbon NMR spectra were found almost similar for the control and the treated samples. The isotopic abundance ratio analysis revealed that the isotopic abundance ratio of PM+1/PM (2H/1H or 13C/12C or 17O/16O or 25Mg/24Mg) in two magnesium gluconate ion forms at m/z 447 and 415 in treated sample was significantly decreased by 59.82% and 55.44%, respectively compared with the control sample. The percentage change in the isotopic abundance ratio of PM+2/PM (18O/16O or 26Mg/24Mg) was remarkably decreased in the magnesium gluconate ion at m/z 447 in the treated sample by 78.26% compared with the control sample. Consequently, the isotopic abundance ratio of PM+1/PM (2H/1H or 13C/12C or 17O/16O) in gluconate ion in the treated sample was significantly increased by 37.35% with respect to the control sample. Thus, the Trivedi Effect® treated magnesium gluconate could be valuable for designing better pharmaceutical and/or nutraceutical formulations through its changed physicochemical and thermal properties, which might be providing better therapeutic response against various diseases such as diabetes mellitus, allergies, aging, inflammatory diseases, immunological disorders, and other chronic infections. The treated magnesium gluconate might be helpful to design the novel potent enzyme inhibitors by using its kinetic isotope effects.
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LC-MS and NMR Based Structural Characterization and Isotopic Abundance Ratio Analysis of Magnesium Gluconate Treated with the Consciousness Energy Healing, 2017
Magnesium gluconate is widely used pharmaceutical/nutraceutical compound for the prevention and treatment of magnesium deficiency diseases. The present study was designed to explore the effect of The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) on magnesium gluconate for the change in the structural properties and isotopic abundance ratio (PM+1/PM and PM+2/PM) using LC-MS and NMR spectroscopy. Magnesium gluconate was divided into two parts – one part was control, and another part was treated with The Trivedi Effect® - Energy of Consciousness Healing Treatment remotely by twenty renowned Biofield Energy Healers and defined as The Trivedi Effect® treated sample. The LC-MS analysis of both the control and Biofield Energy Treated samples indicated the presence of mass of the protonated magnesium gluconate at m/z 415 at the retention time of 1.52 min and fragmentation pattern of both samples were almost identical. The relative peak intensities of the fragment ions were significantly altered in the treated sample compared to the control sample. The proton and carbon signals for CH, CH2 and CO groups in the proton and carbon NMR spectra of the control and treated samples were found same. The percentage change in the isotopic abundance ratio of PM+1/PM (2H/1H or 13C/12C or 17O/16O or 25Mg/24Mg) was significantly decreased in the treated sample by 48.87% compared to the control sample. Subsequently, the isotopic abundance ratio of PM+2/PM (18O/16O or 26Mg/24Mg) in the treated sample was significantly increased by 29.18% compared with the control sample. In summary, 13C, 2H, 17O, and 25Mg contributions from (C12H23MgO14)+ to m/z 416; 18O and 26Mg contributions from (C12H23MgO14)+ to m/z 417 in the treated sample were significantly altered compared with the control sample. Thus, The Trivedi Effect® Treated magnesium gluconate might be helpful to design the novel potent enzyme inhibitors using its kinetic isotope effects. Consequently, The Trivedi Effect® Treated magnesium gluconate would be valuable for designing better pharmaceutical and/or nutraceutical formulations through its altered physicochemical and thermal properties, which might be providing better therapeutic response against various diseases such as diabetes mellitus, allergy, aging, inflammatory diseases, immunological disorders, and other chronic infections.
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Structural Properties and Isotopic Abundance Ratio Analysis of Magnesium Gluconate Treated with the Energy of Consciousness Using LC-MS and NMR Spectroscopy, 2017
The present study was aimed to evaluate the impact of The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) on magnesium gluconate for the change in the structural properties and isotopic abundance ratio (PM+1/PM and PM+2/PM) using LC-MS and NMR spectroscopy. Magnesium gluconate was divided into two parts – one part was control, and another part was treated with The Trivedi Effect®- Biofield Energy Healing Treatment remotely by twenty renowned Biofield Energy Healers and defined as The Trivedi Effect® treated sample. The LC-MS analysis of both the control and treated samples revealed the presence of the mass of the protonated magnesium gluconate at m/z 415 at the retention time of 1.52 min with almost similar fragmentation pattern. The relative peak intensities of the fragment ions of the treated sample were significantly altered compared with the control sample. The proton and carbon signals for CH, CH2 and CO groups in the proton and carbon NMR spectra were found almost similar for the control and the treated samples. The isotopic abundance ratio analysis revealed that the isotopic abundance ratios of PM+1/PM (2H/1H or 13C/12C or 17O/16O or 25Mg/24Mg) and PM+2/PM (18O/16O or 26Mg/24Mg) were significantly decreased in the treated sample by 35.97% and 66.77%, respectively compared with the control sample. Briefly, 13C, 2H, 17O, and 25Mg contributions from (C12H23MgO14)+ to m/z 416; 18O and 26Mg contributions from (C12H23MgO14)+ to m/z 417 in the treated sample were significantly reduced compared with the control sample. Thus, The Trivedi Effect® treated magnesium gluconate could be valuable for designing better pharmaceutical and/or nutraceutical formulations through its changed physicochemical and thermal properties, which might be providing better therapeutic response against various diseases such as diabetes mellitus, allergy, aging, inflammatory diseases, immunological disorders, and other chronic infections. The Trivedi Effect® Treated magnesium gluconate might be supportive to design the novel potent enzyme inhibitors using its kinetic isotope effects.
Michael Ellis, Karen Byrnes Allen, James Peoples, James Joseph Meuer, Judy Weber, John Lawrence Griffin, John Kei Suzuki, Julia Grace McCammon, Madeline Michaels, Johanne Dodon, Kathryn Sweas, Lisa Ann Knoll, Viviane J.(Kris) Elig, Muriel Mae Ranger, Margaret Kweya Wahl, Paromvong Sinbandhit, Joseph Foty, Lezley Wright, Mark Edward Stutheit, Michelle Lynn Barnard, Alice Branton, Mahendra Kumar Trivedi, Dahryn Trivedi
Evaluation of the Impact of the Trivedi Effect® -Energy of Consciousness on the Structure and Isotopic Abundance Ratio of Magnesium Gluconate Using LC-MS and NMR Spectroscopy, 2017
Magnesium gluconate is a classical pharmaceutical/nutraceutical compound used as a magnesium ion source for the prevention and treatment of hypomagnesemia. The present study was aimed to investigate the effect of The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) on magnesium gluconate for the change in the structural properties and isotopic abundance ratio (PM+1/PM and PM+2/PM) using LC-MS and NMR spectroscopy. Magnesium gluconate was divided into two parts – one part was control, and another part was treated with The Trivedi Effect® - Biofield Energy Healing Treatment remotely by twenty renowned Biofield Energy Healers and defined as The Trivedi Effect® Treated sample. The LC-MS analysis of both the control and treated samples indicated the presence of mass of the protonated magnesium gluconate at m/z 415 at the retention time of 1.52 min and fragmentation pattern of the both sample were almost similar. The relative peak intensities of the fragment ions were significantly changed in the treated sample compared with the control sample. The proton and carbon signals for CH, CH2 and CO groups in the proton and carbon NMR spectra were observed almost similar for the control and the treated samples. The percentage change in the isotopic abundance ratio of PM+1/PM (2H/1H or 13C/12C or 17O/16O or 25Mg/24Mg) was significantly decreased in the treated sample by 17.51% compared with the control sample. Consequently, the isotopic abundance ratio of PM+2/PM (18O/16O or 26Mg/24Mg) in the treated sample was significantly increased by 79.44% compared to the control sample. Briefly, 13C, 2H, 17O, and 25Mg contributions from (C12H23MgO14)+ to m/z 416; 18O and 26Mg contributions from (C12H23MgO14)+ to m/z 417 in treated sample were significantly altered compared with the control sample. Thus, The Trivedi Effect® Treated magnesium gluconate might be supportive to design the novel potent enzyme inhibitors using its kinetic isotope effects. Consequently, The Trivedi Effect® Treated magnesium gluconate would be valuable for designing better pharmaceutical and/or nutraceutical formulations through its changed physicochemical and thermal properties, which might be providing better therapeutic response against various diseases such as diabetes mellitus, allergy, aging, inflammatory diseases, immunological disorders, and other chronic infections.
Influence of the Consciousness Energy Healing Treatment on the Structural Properties and Isotopic Abundance Ratio of Magnesium Gluconate, 2021
"Magnesium is a second most abundant mineral in the human body and, which is required for in more than 300 enzymatic reactions, normal functioning of body organs, etc. The main objective of the current study was to investigate the impact of The Trivedi Effect®- Consciousness Energy Healing Treatment on magnesium gluconate for the change in the isotopic abundance ratio (PM+1/PM and PM+2/PM) along with the structural properties using LC-MS and NMR spectroscopy. Magnesium gluconate sample was divided into two parts – one part termed as control, while the other part was treated with The Trivedi Effect®- Biofield Energy Healing Treatment by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi and termed as Biofield Energy Treated sample. The LC-MS analysis of the control and Biofield Energy Treated samples exhibited the presence of the molecular mass of magnesium gluconate adduct with hydrogen ion at m/z 415 (calcd for C12H23MgO14+, 415.09) at the retention time of 1.67 minutes and almost similar fragmentation pattern. But, the relative peak intensities of the fragmented ions of the Biofield Energy Treated sample were significantly changed compared with the control sample. The proton and carbon signals for CH, CH2, OH, and CO groups in the 1H and 13C NMR spectra of the treated sample were almost close compared with the control sample. The isotopic abundance ratio of PM+1/PM (2H/1H or 13C/12C or 17O/16O or 25Mg/24Mg) in the treated magnesium gluconate was significantly decreased by 60.41% compared to the control sample. Therefore, the 13C, 2H, 17O, and 25Mg contributions from (C12H23MgO14)+ to m/z 416 in the treated sample was significantly decreased compared to the control sample. Similarly, the isotopic abundance ratio of PM+2/PM (18O/16O or 26Mg/24Mg) in the treated sample was significantly decreased by 79.33% compared to the control sample. Briefly, the 18O and 26Mg contributions from (C12H23MgO14)+ to m/z 417 in the treated magnesium gluconate significantly decreased compared with the control sample. Thus, The Trivedi Effect®-Consciousness Energy Healing Treated magnesium gluconate might be supportive to design the novel potent enzyme inhibitors and better pharmaceutical and nutraceutical formulations through its changed intrinsic properties. This Biofield Energy Treated magnesium gluconate could prove better therapeutic response against various diseases such as diabetes mellitus, aging, allergy, immunological disorders, inflammatory diseases, and other chronic infections."
Isotopic Abundance Ratio Analysis and Structural Characterization of the Consciousness Energy of Healing Treated Magnesium Gluconate, 2019
Magnesium gluconate is the best source of magnesium for the human body, which is required for the metabolic processes, normal functioning of nerves, cells, muscles, bones, heart, etc. The aim of this research was to investigate the impact of the Trivedi Effect® on Mg-gluconate on the structural properties and isotopic abundance ratio using LC-MS and NMR spectroscopy. Magnesium gluconate sample was divided into two parts – one part was a control sample, and another part was received the Biofield Energy Healing Treatment by a famous Biofield Energy Healer, Mr. Mahendra Kumar Trivedi is known as treated sample. The LC-MS of both the samples revealed the presence of protonated magnesium gluconate mass [M+H]+ peak at m/z 415 (calcd for C12H23MgO14+, 415.09) at the retention time of 1.68 minutes. However, the relative peak intensities of the Biofield Energy Treated sample were significantly altered compared with the control sample. The isotopic abundance ratio of PM+2/PM (26Mg/24Mg or 18O/16O) was significantly increased by 58%, in the treated sample compared with the control sample. Briefly, 18O and 26Mg contributions from C12H23MgO14+ to m/z 417 in the Biofield Energy Treated magnesium gluconate was significantly increased compared with the control sample. The proton and carbon signals for OH, CH, CH2 and CO groups in the proton and carbon NMR spectra of the Biofield Energy Treated sample were almost similar compared to the control sample. The improvement in the isotopic abundance ratios of the treated magnesium gluconate might be due to the possible mediation of neutrinos via the Trivedi Effect®-Consciousness Energy Healing Treatment. The increased isotopic abundance ratio of the treated magnesium gluconate might improve strong atomic bond, increase the stability, and alter the rate of metabolic reactions in the body. Thus, the Biofield Energy Treated magnesium gluconate would be supportive to design the novel potent enzyme inhibitors using its kinetic isotope effects. Similarly, the Biofield Energy Treated magnesium gluconate could be valuable for designing better pharmaceutical and nutraceutical formulations, which might provide better therapeutic response against various diseases such as diabetes mellitus, aging, allergy, immunological disorders, inflammatory diseases, and other chronic infections.
American Journal of Biomedical and Life Sciences, 2017
Magnesium gluconate is a classical pharmaceutical/nutraceutical compound used as a magnesium ion source for the prevention and treatment of hypomagnesemia. The present study was aimed to investigate the effect of The Trivedi Effect ®-Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) on magnesium gluconate for the change in the structural properties and isotopic abundance ratio (P M+1 /P M and P M+2 /P M) using LC-MS and NMR spectroscopy. Magnesium gluconate was divided into two parts-one part was control, and another part was treated with The Trivedi Effect ®-Biofield Energy Healing Treatment remotely by twenty renowned Biofield Energy Healers and defined as The Trivedi Effect ® Treated sample. The LC-MS analysis of both the control and treated samples indicated the presence of mass of the protonated magnesium gluconate at m/z 415 at the retention time of 1.52 min and fragmentation pattern of the both sample were almost similar. The relative peak intensities of the fragment ions were significantly changed in the treated sample compared with the control sample. The proton and carbon signals for CH, CH 2 and CO groups in the proton and carbon NMR spectra were observed almost similar for the control and the treated samples. The percentage change in the isotopic abundance ratio of P M+1 /P M (2 H/ 1 H or 13 C/ 12 C or 17 O/ 16 O or 25 Mg/ 24 Mg) was significantly decreased in the treated sample by 17.51% compared with the control sample. Consequently, the isotopic abundance ratio of P M+2 /P M (18 O/ 16 O or 26 Mg/ 24 Mg) in the treated sample was significantly increased by 79.44% compared to the control sample. Briefly, 13 C, 2 H, 17 O, and 25 Mg contributions from (C 12 H 23 MgO 14) + to m/z 416; 18 O and 26 Mg contributions from (C 12 H 23 MgO 14) + to m/z 417 in treated sample were significantly altered compared with the control sample. Thus, The Trivedi Effect ® Treated magnesium gluconate might be supportive to design the novel potent enzyme inhibitors using its kinetic isotope effects. Consequently, The Trivedi Effect ® Treated magnesium gluconate would be valuable for designing better pharmaceutical and/or nutraceutical formulations through
Evaluation of Structural Properties and Isotopic Abundance Ratio of Biofield Energy Treated (The Trivedi Effect®) Magnesium Gluconate Using LC-MS and NMR, 2017
The current research work was designed to explore the impact of The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) on magnesium gluconate for the change in the structural properties and isotopic abundance ratio (PM+1/PM and PM+2/PM) by using LC-MS and NMR spectroscopy. Magnesium gluconate was divided into two parts – one part was control, and another part was treated with The Trivedi Effect®- Biofield Energy Healing Treatment remotely by seven renowned Biofield Energy Healers and defined as The Trivedi Effect® Treated sample. The LC-MS analysis of the both control and treated samples revealed the presence of the mass of the protonated magnesium gluconate at m/z 415 at the retention time of 1.53 min with similar fragmentation pattern. The relative peak intensities of the fragment ions of the treated sample were significantly changed compared with the control sample. The proton and carbon signals for CH, CH2 and CO groups in the proton and carbon NMR spectra were found almost similar for the control and the treated samples. The isotopic abundance ratio analysis revealed that the isotopic abundance ratio of PM+1/PM (2H/1H or 13C/12C or 17O/16O or 25Mg/24Mg) in the treated sample was significantly increased by 34.33% compared with the control sample. Subsequently, the percentage change of the isotopic abundance ratio of PM+2/PM (18O/16O or 26Mg/24Mg) was significantly decreased in the treated sample by 64.08% as compared to the control sample. Briefly, 13C, 2H, 17O, and 25Mg contributions from (C12H23MgO14)+ to m/z 416; 18O and 26Mg contributions from (C12H23MgO14)+ to m/z 417 in the treated sample were significantly changed compared with the control sample. Thus, the treated magnesium gluconate could be valuable for designing better pharmaceutical and/or nutraceutical formulations through its changed physicochemical and thermal properties, which might be providing better therapeutic response against various diseases such as diabetes mellitus, allergy, aging, inflammatory diseases, immunological disorders, and other chronic infections. The Biofield Energy Treated magnesium gluconate might be supportive to design the novel potent enzyme inhibitors by using its kinetic isotope effects.
European Journal of Biophysics, 2017
The current research work was designed to explore the impact of The Trivedi Effect ®-Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) on magnesium gluconate for the change in the structural properties and isotopic abundance ratio (P M+1 /P M and P M+2 /P M) by using LC-MS and NMR spectroscopy. Magnesium gluconate was divided into two parts-one part was control, and another part was treated with The Trivedi Effect ®-Biofield Energy Healing Treatment remotely by seven renowned Biofield Energy Healers and defined as The Trivedi Effect ® Treated sample. The LC-MS analysis of the both control and treated samples revealed the presence of the mass of the protonated magnesium gluconate at m/z 415 at the retention time of 1.53 min with similar fragmentation pattern. The relative peak intensities of the fragment ions of the treated sample were significantly changed compared with the control sample. The proton and carbon signals for CH, CH 2 and CO groups in the proton and carbon NMR spectra were found almost similar for the control and the treated samples. The isotopic abundance ratio analysis revealed that the isotopic abundance ratio of P M+1 /P M (2 H/ 1 H or 13 C/ 12 C or 17 O/ 16 O or 25 Mg/ 24 Mg) in the treated sample was significantly increased by 34.33% compared with the control sample. Subsequently, the percentage change of the isotopic abundance ratio of P M+2 /P M (18 O/ 16 O or 26 Mg/ 24 Mg) was significantly decreased in the treated sample by 64.08% as compared to the control sample. Briefly, 13 C, 2 H, 17 O, and 25 Mg contributions from (C 12 H 23 MgO 14) + to m/z 416; 18 O and 26 Mg contributions from (C 12 H 23 MgO 14) + to m/z 417 in the treated sample were significantly changed compared with the control sample. Thus, the treated magnesium gluconate could be valuable for designing better pharmaceutical and/or nutraceutical formulations through its changed physicochemical and thermal properties, which might be providing better therapeutic response against various diseases such as diabetes mellitus, allergy, aging, inflammatory diseases, immunological disorders, and other chronic infections. The Biofield Energy Treated magnesium gluconate might be supportive to design the novel potent enzyme inhibitors by using its kinetic isotope effects.
Effect of the Energy of Consciousness (The Trivedi Effect®) on the Structural Properties and Isotopic Abundance Ratio of Magnesium Gluconate Using LC-MS and NMR Spectroscopy. Abstract: Magnesium gluconate has the wide application for the prevention and treatment of hypomagnesemia. The objective of the current study was to investigate the effect of The Trivedi Effect ®-Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) on magnesium gluconate for the change in the structural properties and isotopic abundance ratio (P M+1 /P M) using LC-MS and NMR spectroscopy. Magnesium gluconate was divided into two parts – one part was control, and another part was treated with The Trivedi Effect ®-Biofield Energy Healing Treatment remotely by seven renowned Biofield Energy Healers and defined as The Trivedi Effect ® Treated sample. The LC-MS analysis of both the control and treated samples revealed the presence of the mass of the protonated magnesium gluconate at m/z 415 at the retention time of 1.53 minutes with almost similar fragmentation pattern. The relative peak intensities of the fragment ions of the treated sample were significantly altered compared with the control sample. The proton and carbon signals for CH, CH 2 and CO groups in the proton and carbon NMR spectra were observed almost similar for the control and the treated samples. The isotopic abundance ratio analysis revealed that the percentage of the isotopic abundance ratio of P M+1 /P M (2 H/ 1 H or 13 C/ 12 C or 17 O/ 16 O or 25 Mg/ 24 Mg) was significantly increased in treated sample by 80.38%, compared with the control sample. Briefly, 13 C, 2 H, 17 O, and 25 Mg contributions from (C 12 H 23 MgO 14) + to m/z 416; 18 O and 26 Mg contributions from (C 12 H 23 MgO 14) + to m/z 417 in the treated sample were significantly increased compared with the control sample. Thus, the treated magnesium gluconate could be valuable for designing better pharmaceutical and/or nutraceutical formulations through its changed physicochemical and thermal properties, which might be providing better therapeutic response against various diseases such as diabetes mellitus, allergy, aging, inflammatory diseases, immunological disorders, and other chronic infections. The Trivedi Effect ® treated magnesium gluconate might be supportive to design the novel potent enzyme inhibitors using its kinetic isotope effects. 8 Mahendra Kumar Trivedi et al.: Effect of the Energy of Consciousness (The Trivedi Effect ®) on the Structural Properties and Isotopic Abundance Ratio of Magnesium Gluconate Using LC-MS and NMR Spectroscopy