Sequence analysis of the 16s rRNA gene of sulfate-reducing bacteria isolated from human intestine (original) (raw)
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Journal of Clinical Microbiology, 2001
Strain FH26001/95 (ATCC 700045) was previously isolated from a pyogenic liver abscess from a human. Comparative 16S rRNA gene sequence analysis showed that this strain is related to members of the delta subgroup of the proteobacteria, within a cluster of sulfate-reducing bacteria (Desulfovibrio spp.) and nonsulfate-reducing bacteria (Bilophila wadsworthia and Lawsonia spp.). The phenotype of strain FH26001/95 was found to be typical of members of the genus Desulfovibrio. Growth and substrate transformations were possible at oxygen concentrations of 2 to 5% (vol/vol) but not at oxygen concentrations of 21% (vol/vol) in air. Its isolation from an infection in a human suggests that some members of the genus Desulfovibrio can be considered opportunistic pathogens.
International Journal of Systematic and Evolutionary Microbiology, 2009
Desulfovibrio paquesii sp. nov., a hydrogenotrophic sulfate-reducing bacterium isolated from a synthesis-gas-fed bioreactor treating zinc-and sulfate-rich wastewater A hydrogenotrophic, sulfate-reducing bacterium, designated strain SB1 T , was isolated from sulfidogenic sludge of a full-scale synthesis-gas-fed bioreactor used to remediate wastewater from a zinc smelter. Strain SB1 T was found to be an abundant micro-organism in the sludge at the time of isolation. Hydrogen, formate, pyruvate, lactate, malate, fumarate, succinate, ethanol and glycerol served as electron donors for sulfate reduction. Organic substrates were incompletely oxidized to acetate. 16S rRNA gene sequence analysis showed that the closest recognized relative to strain SB1 T was Desulfovibrio gigas DSM 1382 T (97.5 % similarity). The G+C content of the genomic DNA of strain SB1 T was 62.2 mol%, comparable with that of Desulfovibrio gigas DSM 1382 T (60.2 mol%). However, the level of DNA-DNA relatedness between strain SB1 T and Desulfovibrio gigas DSM 1382 T was only 56.0 %, indicating that the two strains are not related at the species level. Strain SB1 T could also be differentiated from Desulfovibrio gigas based on phenotypic characteristics, such as major cellular fatty acid composition (anteiso-C 15 : 0 , iso-C 14 : 0 and C 18 : 1 cis 9) and substrate utilization. Strain SB1 T is therefore considered to represent a novel species of the genus Desulfovibrio, for which the name Desulfovibrio paquesii sp. nov. is proposed. The type strain is SB1 T (5DSM 16681 T 5JCM 14635 T ).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2013
A novel anaerobic, chemo-organotrophic, sulfate-reducing bacterium, designated strain Olac 40 T , was isolated from a Tunisian wastewater digestor. Cells were curved, motile rods or vibrios (5.0-7.0¾0.5 mm). Strain Olac 40 T grew at temperatures between 15 and 50 6C (optimum 40 6C), and between pH 5.0 and 9.0 (optimum pH 7.1). It did not require NaCl for growth but tolerated it up to 50 g l "1 (optimum 2 g l "1 ). In the presence of sulfate or thiosulfate, strain Olac 40 T used lactate, pyruvate and formate as energy sources. Growth was observed on H 2 only in the presence of acetate as carbon source. In the presence of sulfate or thiosulfate, the end products of lactate oxidation were acetate, sulfide and CO 2 . Sulfate, thiosulfate and sulfite were used as terminal electron acceptors, but not elemental sulfur, nitrate or nitrite. The genomic DNA G+C content of strain Olac 40 T was 70 mol%. The profile of polar lipids consisted of phosphatidylglycerol, phosphatidylethanolamine, aminophospholipid and four phospholipids. The main fatty acids were C 16 : 0 , anteiso-C 15 : 0 and iso-C 15 : 0 . Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain Olac 40 T was affiliated with the family Desulfovibrionaceae within the class Deltaproteobacteria. On the basis of 16S rRNA gene sequence comparisons and physiological characteristics, strain Olac 40 T is proposed to be assigned to a novel species of the genus Desulfocurvus, for which the name Desulfocurvus thunnarius is proposed. The type strain is Olac 40 T (5DSM 26129 T 5JCM 18546 T ).
Systematic and Applied Microbiology, 1998
A mesophilic strain of sulfate-reducing bacterium, designated ALA-3T (T = type strain), was isolated from an anaerobic lagoon of a dairy wastewater treatment plant. The curved, Gram-negative, nonsporeforming cells (0.2 x 3.0-4.0 pm) existed singly or in chains, and were motile by single polar flagella. Optimum growth occurred at 35 "C and pH 7.5 on a medium containing lactate and sulfate. Thio-'sulfate or sulfite but not elemental sulfur, nitrate, or fumarate could also replace sulfate as an electron acceptor. Formate, alanine, aspartate, leucine, isoleucine, valine, and methionine, H2/C02 and ethanol also served as electron donors with sulfate as an electron acceptor. Pyruvate, casamino acids, peptone, serine, glycine, cysteine and threonine were fermented. Sulfite and thiosulfate were disproportionated to sulfate and sulfide. The G+C content of the DNA was 66 mol % G+C. Phylogenetic analysis revealed that Desulfovibrio africanzts was the nearest relative (similarity of 89%). Strain ALA-3T is physiologically and phylogenetically different from other Desztlfouibrio species, and is designated Desulfovibrio aminophilus sp. nov. (DSM 12254).
Isolation and Characterisation of a Novel Sulphate-reducing Bacterium of the Desulfovibrio Genus
Anaerobe, 1998
A novel sulphate-reducing bacterium (Ind 1) was isolated from a biofilm removed from a severely corroded carbon steel structure in a marine environment. Light microscopy observations revealed that cells were Gramnegative, rod shaped and very motile. Partial 16S rRNA gene sequencing and analysis of the fatty acid profile demonstrated a strong similarity between the new species and members from the Desulfovibrio genus. This was confirmed by the results obtained following purification and characterisation of the key proteins involved in the sulphate-reduction pathway. Several metal-containing proteins, such as two periplasmic proteins: hydrogenase and cytochrome c 3 , and two cytoplasmic proteins: ferredoxin and sulphite reductase, were isolated and purified. The latter proved to be of the desulfoviridin type which is typical of the Desulfovibrio genus. The study of the remaining proteins revealed a high degree of similarity with the homologous proteins isolated from Desulfovibrio gigas. However, the position of the strain within the phylogenetic tree clearly indicates that the bacterium is closely related to Desulfovibrio gabonensis, and these three strains form a separate cluster in the delta subdivision of the Proteobacteria.
The aim and background of the study were to investigate bacterial growth of the Desulfomicrobium sp. strains from human large intestine, and to study the processes usage of sulfate- and lactate, production of sulfide and acetate by the obtained bacteria as well as to carry out cluster and correlation analysis of these processes. Microbiology methods of the study for bacterial strains cultivation and photometric methods for determination of bacterial biomass and hydrogen sulfide concentration were used, sulfate ions concentration was determined by turbidymetric method, lactate concentration was carried out by lactate dehydrogenase. Acetate ions accumulation by the strains was determined by titration. Using the experimental data, the methods of statistical analysis have been also used. The highest biomass (3.34 g/l) has been accumulated by the Desulfomicrobium sp. strain Rod-9 on the sixth day of cultivation, in comparison to the other strains. Studied strains of the Desulfomicrobium sp. actively reduced the sulfate ions and produced hydrogen sulfide. The highest concentration of hydrogen sulfide (3.14 mM) was produced by the SRB Rod-9 strain on the eighth day of cultivation; while, the strain used about 98% of the sulfate compared with its initial concentration. The strains used lactate fully on the sixth day of cultivation and produced acetate. Clustering of the parameters of the bacterial growth, the sulfate and lactate usage as well as hydrogen sulfide and acetate production by the various Desulfomicrobium sp. strains has been carried out. The correlation coefficients between the parameters have been determined. Keywords: Sulfate-reducing bacteria, Desulfomicrobium , intestinal microbiocenosis, sulfates, sulfide, inflammatory bowel diseases
Desulfovibrio simplex spec. nov., a new sulfate-reducing bacterium from a sour whey digester
Archives of Microbiology, 1989
Desulfovibrio simplex spec. nov. strain XVI was iso la ted from an anaerobic sour whey digester. Single ceHs had a vibrioid shape and were motile by a single, polar f1agellurn. The size of cells was 0.5 1.0 j.tm x 1.5-3.0 j.tm. The G+C content of the DNA of D. simplex strain XVI was 47.5 mol%. The only other Desulfovibrio species with a similar G+C content of the DNA was Desulfovibrio salexigens (46.1 mol%), while that of all other species was between 55 and 64 mol%. D. simplex grew on Hz/CO z , formate, pyruvate, L(+)-lactate, fumarate, malate, ethanol, 1-propanol and i-butanol as electron donors, while De sulfovibrio salexigens grew in addition on methanol, 2-pro panol, 2-butanol, glycerol, suceinate, citrate, choline and glucose. Electron acceptors for D. simplex were sulfate, thio sulfate and nitrate. L(+)-Lactate was incompletely oxidized to acetate and CO z during sulfate reduction. Furthermore, both species could be distinguished by the ability of D. simplex but not ofD. salexigens to grow on and to oxidize benzaldehyde derivatives to the respective acids, including vanillin, p-anisaldehyde and syringaldehyde. Moreover, D. simplex could grow in the presence of trace amounts of NaCI, while D. salexigens had an obligate requirement of 25 g/l NaCL In addition, D. simplex can be distinguished from D. salexigens by its differing polyamine pattern. On the basis of the presented data the description of strain XVI as Desulfovibrio simplex spec. nov. is proposed.