Isolation and characterization of diesel oil degrading indigenous microrganisms in Kwazulu-Natal, South Africa (original) (raw)
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Exploiting bacterial isolates for diesel degrading potential under in vitro conditions
Plant Science Today, 2022
Hydrocarbon contaminated oil-spilled areas and oil-products have caused serious harm with increasing attention for development, implementation and removal of these contaminants. Bacterial diversity on succession at the petroleum hydrocarbon contaminated environment can give answer the problem. Such lands have serious problems as totally barren or with rare plantation. Bacteria can thereby be exploited for the mitigation of hydrocarbon to enhance the nutrient availability for vegetation. Present study involves collection of soil samples heavily contaminated with hydrocarbon from Bagru (Rajasthan). Samples were analysed by solid liquid extraction method followed by FTIR (Fourier Transform Infrared) and HPLC (High Performance Liquid Chromatography) analysis. During microbiological analysis hydrocarbon degrading bacteria were screened. FTIR spectral analysis indicated the presence of the functional group's alkanes and aromatic ringed compounds; 43% to 69% hydrocarbon content recorded by HPLC analysis of all the soil samples respectively. From the soil samples six gram-positive and four gram-negative bacterial isolates were explored possessing hydrocarbon degrading capacities in the range 47.04-87.31% and 10.12-95.24% respectively. Growth kinetic studies revealed the degradation up to 1000 ppm diesel in 3 days under in vitro conditions. These bacteria can further be exploited for diesel degradation and will certainly propose a possible solution to the prevailing issue for its biodegradation in ex-situ conditions after up scaling.
The present work was conducted to isolate and identify bacteria from oil- 21 contaminated soil to evaluate their role in biodegradation of commercial diesel under laboratory 22 conditions. Diesel fuels are used by different vehicles, diesel generators and especially heavy 23 transport vehicles. Its manufacturing, transportation, utilization and disposal have the threat to 24 pollute the surrounding environment. Biodegradation is one of the biological processes to 25 remediate the pollutants. This is the cheaper and easy method as compared to other methods like 26 direct burning, land foaming and bioventing because diesel smoke has a carcinogenic effect. 27 In the current exploration ordinary bacterial species that degrade diesel were isolated from 28 selected areas of Mansehra. Six sites were selected and a total of 60 samples were collected. 29 These sites were coded according to their names. For the isolation of diesel degrading bacteria 30 soil samples were analyzed. The isolation of bacteria was done on nutrient agar, nutrient broth 31 and mineral salt medium (MSM), and IR spectroscopy was used to observe the degradation of 32 diesel. Twenty samples were found having bacterial growth. Further analysis showed that four 33 samples BHG, SHNK, TAK and KMOR had diesel degrading capability as these showed 34 positive results. The isolated bacteria were identified by morphological and biochemical features, 35 while the degradation of diesel was determined qualitatively by interpreting the intensity of the 36 peaks of IR spectroscopy. The intensity of the peaks in the spectrum of control was compared 37 with the intensity of the peaks in the spectrum of the test samples which indicated degradation of 38 diesel. Three bacterial species, namely, Bacillus cereus, Bacillus subtilis and Pseudomonas 39 aeruginosa were identified using Berge's manual method. The bacterial species were tested for 40 their capability to degrade commercial diesel presented at different concentrations i.e. 1000 ppm 41 and 5000 ppm.
Isolation and Identification of Diesel oil-degrading Bacteria in used engine oil contaminated soil
Journal of Applied Science and Environmental Management, 2019
This study was conducted to isolate and identify bacteria associated with spent diesel oil contaminated site by collecting three replicates contaminated soil from the works department of Admiralty University of Nigeria, while the pristine soil was obtained from the biological garden. pH and temperature of all the samples were checked and the bacteria isolates were identified by morphological and biochemical techniques using the taxonomy scheme of Bergey's Manual of determinative bacteriology. The pH and temperature was highest (6.06 to 6.26) and (27 to 30 0 C) in the contaminated soil than the pristine soil (5.83) and (23 0 C). The mean microbial load count was highest (10.0x10 5 cfu/ml)in the diesel oil contaminated soils than the pristine uncontaminated soils (9.0x0x10 5 cfu/ml). However, there was no significant difference between the microbial loads in the soil samples. Eight bacteria species were obtained from the contaminated soil while six were obtained from the pristine soil. Klebsiella spp., Enterobacterae riginosa, Pseudomonas sp. and Escherichia coli showed highest growth in the contaminated soil and therefore may be considered as effective in the degradation of spent diesel oil hydrocarbons. It is likely that these bacteria when employed independently may be used to degrade hydrocarbons. Further research is necessary to assay for the degradative ability of the microorganisms.
IRJMETS Publication, 2022
Environmental pollution with petroleum has been recognized amongst the serious threating problems globally. Some of the most existing petroleum hydrocarbons pollutants include diesel, gasoline and fuel oils. In the recent times, bioremediation/biodegradation techniques are used to remove environmental pollutants. A list of microorganisms have the ability to use petroleum hydrocarbons as the sole source of carbon for energy in order to carry out their metabolic activities. It involves the use of microbes to purify and humiliate environmental contaminants. The present study is carried out during 2019-20 by conducting isolation, identification and optimization of diesel degrading microbes from diesel oil polluted in selected sites of Peshawar, Pakistan. Bacteria like Acinetobacter baumani, Acinetobacter pitti, Acinetobacter calcoactius, and Pseudomanas auroginosa were isolated on the basis of morphological and physiological characteristics. Further the optimization was carried out for various culture parameters i.e., Hydrocarbon concentration and pH. The present study suggests that Acinetobacter pitti has the degrading ability at pH 7 when hydrocarbon concentration is kept at 2%.
Isolation and Characterization of Diesel Degrading Bacteria from Petroleum Oil Contaminated Soil
Bacterial Empire
Petroleum products are used for energy production and an essential part of our day-to-day lives especially in vehicles, ships, and industries. Accidental leakages occur easily and wastage petroleum is also discarded in the environment without any further processing causing environmental pollution. Diesel contributea big part topetroleum pollution. The current study was aimed to identify diesel degrading bacteria and determine some conditions to evaluate their best degradation capability. We identified Aeromonas spp., Bacillus spp., and Enterobacter spp. from diesel contaminated soil and found that Aeromonas spp. and Bacillus spp. grow best with 10% to 15% diesel whereas Enterobacter spp. can grow quite well with 20% diesel concentration at a higher temperature (40oC) than the previous two bacteria. Aeromonas spp. worked well at low pH (pH 4 to pH 6) whereas Bacillus spp. and Enterobacter spp. worked best at higher pH (pH 10).
Studies on the Bioremediation Potential of Bacteria Isolated from Diesel-contaminated Soils in Kano
Journal of Biochemistry, Microbiology and Biotechnology
Bioremediation is pollution control technology that uses microorganism to clean up contaminated environment. This study was aimed at assessing the bioremediation potential of bacteria isolated from diesel-contaminated soils and screen them for the ability to remediate diesel contaminated environment using their potential to degrade diesel as carbon and energy source. Diesel-contaminated soil samples were collected using standard method. Bacteria were isolated, characterized and identified using standard microbiological procedures. The identified bacteria species were subsequently screened for diesel biodegradation potential in Bushnell Haas Media (BHM). Optimum conditions (pH, temperature, and diesel concentrations) for biodegradation were determined. The results showed that Bacillus subtilis and Bacillus megaterium were the most potent species identified. Bacillus subtilis and Bacillus megaterium were observed to utilize diesel as the sole carbon source in which they degrade 79% an...
Journal of Biochemistry, Microbiology and Biotechnology
The high rise in the exploration and usage of petroleum products have led to widespread contamination in the environment. Thence, the continuous search for microorganisms with the potential to mineralize these pollutants is necessary. This study was conducted to isolate, identify and determine the diesel degradation potential of bacteria from oil-contaminated soil collected from three filling stations in Azare, Katagum LGA, Bauchi State, Nigeria. The diesel degrading bacteria were identified using standard protocols. The isolates were screened spectrophotometrically for their potential to utilize 1% diesel (v/v) as their sole carbon and energy source and the best candidate was used for determining the effect of diesel concentration on its biodegradation. The results revealed the presence of three bacterial species including Pseudomonas aeruginosa, Pseudomonas putida and Bacillus subtilis. P. Putida showed the highest diesel degradation at 120 h with an absorbance of 2.27±0.03 at 600...
Diversity of diesel degrading bacteria from a hydrocarbon contaminated soil
2014
Hydrocarbon contamination resulting from leaking above ground and underground storage tanks, spillage during transport of petroleum products, abandoned manufactured gas sites and various industrial processes is hazardous to soil and water ecosystem and is expensive to remediate. There is an increased interest in promoting ecofriendly methods in the process of cleaning oil-polluted sites. Biological and non-biological approaches are being used for remediation of oil pollution. Bioremediation is one of the principle strategies for remediation, wherein the pollution can be removed by use of microorganism or by any biological process that uses microorganisms or their enzymes to return the environment altered by contaminants to its original condition (OluArotiowa et al., 2007).
Isolation of bacteria from diesel contaminated soil for diesel remediation
Journal of Bio-Science, 2019
This study is aimed at isolating bacterial species that inhabit diesel contaminated soil and also screened these isolates for the ability to be used for remediating diesel contaminated environment using their potential to degrade diesel as carbon and energy source. Top soil sample was collected from an ancient diesel-powered generator house in Minna, Nigeria, in a sterilized plastic container while diesel oil was obtained from local petrol bunk. Four bacterial isolates were isolated from the diesel contaminated soil sample and were screened for their ability to degrade diesel using mineral salt medium (MSM). The isolates with highest biodegradation potential were identified as Bacillus subtilis and Bacillus cereus. The optimum pH (5, 6, 7 and 8) and hydrocarbon concentration (1%, 2%, 5% and 10%) of the isolate was determined by spectrophotometry and the result revealed that the optimum pH for biodegradation of diesel by Bacillus subtilis and Bacillus cereus, was 7 (1.170) and 8 (1.7...