Chemical and Biological Studies of Leaf Extract of Dendrophthoe falcata Linn (original) (raw)
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Oriental Journal of Chemistry
Dendrophthoe falcata is a hemiparasitic plant that has been used in traditional medicine.The study was conducted to identify the total phenolic and flavonoid compounds, to test antioxidant, and antimicrobial activity of ethanol extract stems of D. falcata parasite on Melia azedarach host tree. The dry powder stems of D. falcata was extracted with ethanol. The ethanol extract was subsequently partitioned successively using n-hexane, chloroform, and ethyl acetate. Each fraction was analyzed by a quantitative phenolic and flavonoid content with spectrophotometer method, and tested as antioxidant and antimicrobial activites. Antioxidant activity was performed by 2,2-diphenyl-1-picrilhidrazil (DPPH) method, while antimicrobial assay used pathogenic bacteria by disk-diffusion method. The results concluded that the stem of D. falcata plant showed a high content of phenolic and flavonoid compounds, very high antioxidant and moderate antimicrobial activities. It was also found that stems of ...
https://www.ijhsr.org/IJHSR\_Vol.8\_Issue.11\_Nov2018/IJHSR\_Abstract.035.html, 2018
In this study, the bioactive compounds of D.falcata leaf and stem have been evaluated using GC-MS techniques. The Chemical composition of the ethanol extract of leaf and stem of D.falcata was, investigated using Perkin – Elmen has chromatography mass spectrometry. This analysis revealed the presence of Dendrophthoe falcata Dibutyl phthalate (13.26%), n-Hexadecanoic acid (13.02%), 1,3,4,5 – Tetrahydroxy-Cyclohexan (8.43%), 2,4-Imidazolidinedione, 1 – [[5-Nitro-2-(uranyl)Methylene] Amino] (6.36%), Z,E-2-Methyl-3, 13-Octadecadien-1-o1 (6.19%), Geranyl linalool Isomer-B (4.82%), Phthalic acid, 5-methylhex-2-yl butyl ester (4.28%), 1,2,3-Benzenetriol (Pyrogallol) (4.17%), 1-Octadecanol (3.99%), 9-Octadecenoic acid (Z) (3.99%), Phthalic acid, bis-(10-hydroxy decyl ester) (3.71%), 2,6,10-Trimethyl, 14-Ethylene-14-pen (3.52%), in the leaf and squalene (68.70%), 1,2-Benzenedicarboxylic acid, Diethyl ester (5.51%), 1,2-Benzenedicarboxylic acid (3.42%), 3-Methylhenicosane (3.15%), Phthalic acid, butyl oct-3-yl ester (2.42%), 2,6-Dimethylbenzenenethiol, S-(tert-butyldimethylsilyl) (1.75%), in the stem. The compounds found in the study are reported to possess antioxidants, anti-inflammatory, anticancer, cytotic, diuretic and antimicrobial activities. The results of this study offer a platform to reconfirm the properties of the components in D.falcata that are used as different ailments.
2020
Abstract: The total phenolic content(TPC) and total flavonoid content(TFC) of chloroform(CEDF), methanol (MEDF) and hydroalcoholic(HEDF) extracts of an Indian ayurvedic plant Dendrophthoe falcata (L.f) Ettingsh were measured using Folin-Ciocalteau and aluminum chloride colorimetric methods, respectively. The in vitro antioxidant activities were also investigated by using in vitro antioxidant models including 2,2´-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS + ), 1,1-diphenyl-2-picrylhydrazyl(DPPH), nitric oxide(NO), hydroxyl radical(OH‾), super oxide(SO) and reducing power (Fe 3+ to Fe 2+ transformation) assay. The TPC were 21.78 ± 3.145, 38.66 ± 1.862 and 46.43 ± 2.55 mg gallic acid equivalents (GAE)/g extract, while TFC were 9.74 ± 1.324, 21.59 ± 1.09 and 33.42 ± 2.083 mg quercetin equivalents (QRT)/g extract sample for CEDF, MEDF and HEDF, respectively. Analysis of the free radical scavenging activities of the fractions revealed a concentration-dependent antiradical ac...
The purpose of this study is to compare the phytochemical evaluation of Dendropthoe falcata (l.f) ettingsh (Loranthaceae) grown on Azardirachta indica and Nerium oleander as host plants. In the Azadirachta indica, the preliminary phytochemical test revealed the presence of alkaloids, reducing sugar, glycosides, flavonoids, tannins and saponins, while in the Nerium oleander, the preliminary phytochemical test revealed the presence of alkaloids, reducing sugar, glycosides, flavonids, tannis, saponin, phlobatannins and phytosterol. The preliminary phytochemical screening of the two host plant, the study revealed that Nerium oleander having better quality phytoconstituents than Azadirchta indica and this attributes to its traditional medicinal use.
Journal of Physics: Conference Series
The aim of the study was to identify the content of total phenolic and flavonoids, and to test the antioxidant activity of leaves extract of Dendrophthoe falcata hemiparasite on Melia azedarach plants. The research method was carried out by extracting dry powder from the leaves of D. falcata with ethanol. Ethanol extract was partitioned using consecutive n-hexane, chloroform, and ethyl acetate solvents. Each fraction was then analyzed for the content of total phenolic and total flavonoids by spectroscopic methods. The method for testing antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH) by in vitro. The results showed that leaves of D. falcata were rich in phenolic compounds and flavonoid. Furthermore, in the antioxidant test showed ethyl acetate fraction had the highest activity, with IC50 6.66 ± 0.11 µg /ml. This study shows that D. falcata leaves have potential as natural antioxidants.
The hydroalcoholic extract (HEDF) from the aerial parts of the hemiparasitic plant D. falcata, was evaluated for acute and subacute toxicity with the hope that the results would provide information on the safety of this extract prior to the evaluation of its efficacy in humans. In rats, single intraperitoneal injections of HEDF (1.0-2.5 g/kg) induced a regular dose-dependent increase in the death rate and incidence of general behaviour adverse effects. In sub acute toxicity studies, three doses of 250, 475 or 950 mg/kg/day of HEDF were administered orally for a period of 28 days. Phytochemical analysis revealed that polyphenols, terpenes and steroids were major compounds. The LD 50 value after acute intraperitoneal doses was 1.75 g/kg. In the open field arena, HEDF (475mg/kg and 950mg/kg p.o.) reduced the number of rearing episodes and locomotion, while number of urine spots increased. In subacute tests, haematological analysis showed a significant (p < 0.01) increase in WBC count. In the blood chemistry analysis, a transient decrease in AST activity was observed whereas cholesterol level was decreases in animals that received high dose of extract. Pathologically, neither gross abnormalities nor histopathological changes were observed. Collectively these data demonstrate that HEDF are relatively safe in rats; however, assessment of hepato-biliary function should be done during chronic use in humans.
In vitro anti-diabetic activity of parasitic plant, Dendrophthoe falcata (L.f) Ettingsh Full Paper
Dendrophthoe falcata; Antidiabetic; Phytochemicals. KEYWORDS ABSTRACT In the present investigation, we have used parasitic plant Dendrophthoe falcata (grows on neem) to analyze phytochemicals and evaluated for in vitro anti-diabetic activity using four different solvent extracts. The aque-ous and methanol extracts yielded saponins, alkaloids, flavonoids, phe-nolic substances at higher concentration compared to other two extracts. The aqueous and methanol extracts also strongly inhibited the important diabetic enzymes (á-amylase, á-glucosidase, â-glucosidase and sucrase) in vitro. Further work is needed to analyse exact antidiabetic compound by separation and biophysical characterization of each compounds. 2013 Trade Science Inc. -INDIA INTRODUCTION
Jurnal Jamu Indonesia, 2020
Petai mistletoe (Dendrophthoe praelonga (Blume) Miq) is semi-parasitic plant. Parasite plant has been used traditionally in Indonesia to prevent or treat degenerative diseases, such as cancer. The purpose of this study was to indentify anticancer compound of petai mistletoe leaves extract and to examine its antioxidant activity (based on DPPH method) and cytotoxic activity on breast cancer (MCF-7) cell lines in vitro using Alamar blue method. This research aims to isolating, characterize, determine antioxidant activity and cytotoxic activity. Separation was conducting column chromathography and characterize was conducted using TLC, FT-IR, LC-MS and NMR-1D. From the result obtained, the isolated compound is flavonol quercetine (3,3,7,3’,4’ pethahydroxyflavone) which active antioxidant with IC50 value of 9.4 μg/mL and the value of cytotoxic activity with IC50 750.62 μg/mL
JPC - Journal of Planar Chromatography - Modern TLC, 2010
Dendrophthoe falcata (Linn. f.) Etting. is a stem parasite commonly known as 'Vanda' in the Ayurvedic system of medicine. A TLC method has been established for simultaneous identification and quantification of phenolic compounds in different parts of this plant. Chromatography was performed on silica gel with tolueneethyl acetate-formic acid 6:4:1 (v/v) as mobile phase, and characteristic bands of (+)-catechin, ellagic acid, quercetin, and ferulic acid were observed at R F 0.35, 0.41, 0.63, and 0.66, respectively. The method was validated for precision, repeatability, and accuracy. The method enables reliable quantification with good resolution and separation from other constituents of the plant extract. The accuracy of the method was checked by measuring recovery of all four phenolic compounds at three different concentrations and by matching the spectra of reference markers with those of the corresponding bands in the extract at three different positions, i.e. the start, apex, and end positions of the band. The presence and quantity of these phenolic compounds varies from part to part of this parasitic plant. For example, quercetin was present only in the leaves, ferulic acid in the stem, and ellagic acid and (+)-catechin in stem and root. More (+)-catechin and ellagic acid were found in the stem than in the root.
Jurnal Pendidikan Kimia, 2018
Research on antimicrobial and toxicity tests of flavonoid total Dendrophthoe pentandra (L) Miq from false ashoke tree (Polyalthia longifolia) have been done. This study aims to determine the activity of toxicity of total flavonoid of Dendrophthoe pentandra (L) Miq as mistletoe of false ashoke tree (P. longifolia) based on Brine Shrimp Lethality Test (BSLT) method using Artemia salina Leach shrimp larvae obtained Lethal Concentration (LC 50) of 30.06 mg / L which means strong toxic activity. Antimicrobial test of flavonoid total has used the diffusion method in order to obtain the inhibit zone diameter on Streptococcus mutans at the total flavonoids concentration of 3%, 6% and 9% were 6; 9 and 17.25 mm, in Escherichia coli is 3.55; 4.25 and 9.15 mm and at Candida albicans is 8.30; 4 and 5.30 mm where S. mutans and E. coli are greater but C. albicans less affect but still have good inhibitory activity so effective to be developed as antimicrobial agents.