The investigation of Helicobacter pylori in the dental biofilm and saliva samples of children with dyspeptic complaints (original) (raw)
Related papers
Salivary PCR detection of Helicobacter pylori DNA in Egyptian patients with dyspepsia
Egyptian Journal of Medical Human Genetics, 2011
Several methods are available for detecting Helicobacter pylori infection: (1) invasive methods based on gastric biopsies, (2) non invasive methods like Urea Breath Test (UBT), serology and stool antigen tests. Importance of salivary PCR in detection of H. pylori is still questionable. To evaluate the role of salivary PCR technique in detecting H. pylori gastric affection in Egyptian patients with
Oral Microbiology and Immunology, 2006
Helicobacter pylori is a gram-negative, curved, microaerophilic organism that has been implicated in the aetiology of gastritis, in the process of gastric and duodenal ulcer formation, and in gastric cancer (15, 17). Approximately 10% of individuals are affected by gastritis and/or gastric ulcer during their lifetime and over 50% of the world's population carries this infection (7). The prevalence of gastric H. pylori infection depends on age and varies strongly between developing and developed countries, and according to ethnicity, place of birth and socioeconomic factors among people living in the same country
The Role of Helicobacter Pylori in Development of Lesion in Oral Cavity
2020
Summary Background/Aim: to examine the connection of H. Pylori in saliva and biopsy material with oral lesions. Material and Methods: Sixty patients with dyspeptic complaints were followed up at the Clinic for Gastroenterology at University Medical Clinical Centre in Skopje, divided into two groups: first group consisted of 30 patients without presence of H. pylori, and the second group with 30 subjects and presence of H. pylori. The presence or absence of H. pylori has been ascertained after endoscopic examination-gastroscopy, and implemented urease test (CLO-test). All patients were clinically followed in order to determinate mouth burning, recurrent aphthous stomatitis (RAS), acid taste and lingual papillary hyperplasia according to Cohen and Proctor. The presence of H. pylori in saliva has been ascertained before endoscopic examination, after chewing Orbit gum without sugar for 1 min using by Pronto dry test. Determination of H. pylori in biopsy material has been ascertained by ...
Detection of Helicobacter pylori DNA in the saliva of patients complaining of halitosis
Journal of Medical Microbiology, 2008
Helicobacter pylori infection, which causes peptic ulcers and gastric cancer, is considered a possible cause of halitosis. Recently, the oral cavity was identified as a possible H. pylori reservoir, particularly in the presence of periodontal disease, which is a cause of halitosis. The purpose of this study was to evaluate by PCR the prevalence of oral H. pylori in the saliva of subjects complaining of halitosis. Samples were obtained from 326 non-dyspeptic subjects, comprising 251 subjects with actual malodour and 75 subjects without halitosis. DNA was extracted from the samples, and the presence of H. pylori and periodontopathic bacteria including Porphyromonas gingivalis, Treponema denticola and Prevotella intermedia was examined by PCR. H. pylori was detected in 21 (6.4 %) of 326 samples. The methyl mercaptan concentration and periodontal parameters including tooth mobility, periodontal pocket depth (PPD) and occult blood in the saliva were significantly greater in the H. pylori-positive subjects. Each of the periodontopathic bacteria was also detected at a significantly higher frequency in the H. pylori-positive subjects. Among those patients with a PPD of ¢5 mm and a tongue coating score of ¡2, no difference was observed in oral malodour levels between the H. pylori-positive and-negative subjects. However, the presence of occult blood in the saliva and the prevalence of Prevotella intermedia were significantly greater in the H. pylori-positive subjects. H. pylori was detected in 16 (15.7 %) of 102 subjects with periodontitis, suggesting that progression of periodontal pocket and inflammation may favour colonization by this species and that H. pylori infection may be indirectly associated with oral pathological halitosis following periodontitis.
2009
Objective: to determine the simultaneous presence of H. pylori in both the oral cavity and gastric mucosal in patients suffering digestive pathologies and to establish the possible association between the presence of H. pylori in the oral cavity and the gingivoperiodontal pathology. Study design: Patients with gastric symptoms (case group) and asymptomatic patients (control group) seen at the Gastroenterology Department of Dr. Julio C. Perrando Hospital (Resistencia, Argentina) were selected. Dental plaque and saliva samples from both groups were obtained. In the case group, gastric biopsy samples were also taken. H. pylori was detected in gastric biopsies by histological stains, and Polymerase Chain Reaction (PCR) was carried out on the oral samples. Results: Among the 98 patients (43 cases and 55 controls), 196 oral samples (saliva 98, dental plaque 98) and 43 gastric biopsias were obtained. H. pylori was detected in oral samples in 18/98 patients, in gastric biopsies in 38/43 patients, and in both samples in 15/43 patients. Conclusions: The presence of H. pylori in the oral cavity of patients suffering digestive pathologies is more frequent in those patients harbouring a gingivoperiodontal disease, and this fact could represent an obstacle for the eradication of the bacterium. At the same time, it could constitute a risk factor for gastrointestinal reinfection after treatment.
Correlation of Helicobacter pylori in dental plaque and gastric mucosa of dyspeptic patients
JPMA. The Journal of the Pakistan Medical Association, 2002
To establish the prevalence of Helicobacter pylori colonization of dental plaque and its correlation with Helicobacter pylori infection of the antral mucosa in patients with symptomatic dyspepsia. Seventy eight adult dyspeptic patients undergoing upper gastrointestinal tract endoscopy were prospectively enrolled. Four air dried dental plaque cytology slides and four gastric antral mucosal biopsies were stained with Giemsa stain. CLO test was used for detection of urease activity of Helicobacter pylori in the dental plaque specimens and antral mucosal biopsies. Data on endoscopic findings and orodental hygiene were recorded. Dental plaque colonization using CLO test and cytology was found to be 100% and 88% respectively. Antral biopsy for H. pylori was positive in 61% cases by CLO test and 57% cases on histopathology. Forty four out of 69 patients (63%) had both dental plaque and antral biopsy positive for H. pylori. No patient with negative dental plaque cytology was positive for H....
Academia Molecular Biology and Genomics, 2024
Introduction: Helicobacter pylori is a Gram-negative bacterium, which has been linked to many gastrointestinal diseases and has been shown to be a causative of gastrointestinal cancer. Aim: The purpose of the study was to identify Helicobacter pylori and its virulence factors (cagA and vacA) by PCR in saliva samples of paediatric patients with abdominal pain. Methodology: A total of 150 patients were included in this study, 74 were females and 76 males. Sublingual saliva was collected, which was inoculated into urea Christensen broth for enrichment, and then PCR was used to amplify the glmM, ureA, cagA and vacA genes. Results: The glmM gene was identified in 60 (40%) of the patients, while the ureA gene was identified in only 3 (2%). In those samples positive for glmM and ureA, the cagA gene was identified in 17 (28.3%) and vacA in 26 (43.3%). Conclusion: Our results show that it is possible to identify H. pylori in saliva of paediatric patients with abdominal pain, this was achieved through subcultures in enrichment media and subsequent PCR of the glmM gene.