Succinic acid production from Bacteroides fragilis: Process optimization and scale up in a bioreactor (original) (raw)
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Theoretical and Experimental Study of Biobased Succinic Acid Production
2017
Biomass based succinic acid is gaining increasing interest as a potential platform chemical for replacing a large petroleum-based bulk chemical market. Biomass as a renewable resource has proved the economic and sustainable potential to produce succinic acid by fermentation method. Biobased succinic acid has yet faced with the challenge of becoming competitive with petrochemical method because of its higher production cost. To lower the production cost, extensive research efforts have been undertaken in upstream technology that involves strain development via metabolic engineering, and downstream technology that aims to improve efficiency of purification method. Many research studies have focused on either one of two technological areas, with little interest on interaction between them. This present work integrates the processing steps from upstream and downstream technologies using a systematic approach and presents an optimal production pathway from a large number of possible process configurations. The development of such a process pathway involves selection of bioproducts, feedstock, pre-treatment technology, microorganism and product separation method. Performance criteria such as titre, rate, yield and minimum production cost, express the optimality of production pathway. Optimization study indicates that succinic acid seems to be the most promising bioproduct among all other bioproducts. Corn stover is the suitable feedstock to produce succinic acid. Based on the findings from optimization study, experimental work was performed with an aim of achieving better performance criteria than it is reported in literature. This work selected corn stover as feedstock, and a bacterium called, Basfia succiniciproducens for converting corn stover-derived glucose into succinic acid. To date, no deliberate experiment has been done on this bacterium to improve succinic acid production, despite its promising features. Highest succinic acid yield of 18 g/100g total
Biotechnology Progress, 2007
Succinic acid (SA) is an important platform molecule in the synthesis of a number of commodity and specialty chemicals. In the present work, dual-phase batch fermentations with the E. coli strain AFP184 were performed using a medium suited for large-scale industrial production of SA. The ability of the strain to ferment different sugars was investigated. The sugars studied were sucrose, glucose, fructose, xylose, and equal mixtures of glucose and fructose and glucose and xylose at a total initial sugar concentration of 100 g L−1. AFP184 was able to utilize all sugars and sugar combinations except sucrose for biomass generation and succinate production. For sucrose as a substrate no succinic acid was produced and none of the sucrose was metabolized. The succinic acid yield from glucose (0.83 g succinic acid per gram glucose consumed anaerobically) was higher than the yield from fructose (0.66 g g−1). When using xylose as a carbon source, a yield of 0.50 g g−1 was obtained. In the mixed-sugar fermentations no catabolite repression was detected. Mixtures of glucose and xylose resulted in higher yields (0.60 g g−1) than use of xylose alone. Fermenting glucose mixed with fructose gave a lower yield (0.58 g g−1) than fructose used as the sole carbon source. The reason is an increased pyruvate production. The pyruvate concentration decreased later in the fermentation. Final succinic acid concentrations were in the range of 25–40 g L−1. Acetic and pyruvic acid were the only other products detected and accumulated to concentrations of 2.7–6.7 and 0–2.7 g L−1. Production of succinic acid decreased when organic acid concentrations reached approximately 30 g L−1. This study demonstrates that E. coli strain AFP184 is able to produce succinic acid in a low cost medium from a variety of sugars with only small amounts of byproducts formed.
Anaerobe, 2007
A statistical approach response surface methodology (RSM) was used to study the production of succinic acid from Bacteroides fragilis. The most influential parameters for succinic acid production obtained through one-at-a-time method were glucose, tryptone, sodium carbonate, inoculum size and incubation period. These resulted in the production of 5.4 g L À1 of succinic acid in 48 h from B. fragilis under anaerobic conditions. Based on these results, a statistical method, face-centered central composite design (FCCCD) falling under RSM was employed for further enhancing the succinic acid production and to monitor the interactive effect of these parameters, which resulted in a more than 2-fold increase in yield (12.5 g L À1 in 24 h). The analysis of variance (ANOVA) showed the adequacy of the model and the verification experiments confirmed its validity. On subsequent scale-up in a 10-L bioreactor using conditions optimized through RSM, 20.0 g L À1 of succinic acid was obtained in 24 h. This clearly indicated that the model stood valid even on large scale. Thus, the statistical optimization strategy led to an approximately 4-fold increase in the yield of succinic acid. This is the first report on the use of FCCCD to improve succinic acid production from B. fragilis. The present study provides useful information about the regulation of succinic acid synthesis through manipulation of various physiochemical parameters.
Indian Journal of Agricultural Research, 2023
Background: The succinic acid demand accelerated through the years. Thus, the need to improve acid production economically is aggravated. The fermentation process by succinate bacteria showed promising acid production. However, due to different bacteria characteristics, mediums composition and operating conditions, a fixed quantity of succinic acid production cannot be determined or generalized. Recently, raising interest in examining cheap mediums in the fermentation process brought attention to utilizing different raw materials. Nevertheless, its influence on production is not fully comprehended. Methods: This research aims to develop an evaluation of three succinate bacteria on succinic acid production. Also, it aims to demonstrate a comparative analysis of the Succinic acid production by three raw carbon sources (Corn fiber, Cane molasses and orange peel). The flask batch method and the 4-L Bioreactor were employed in the evaluation. Result: The results of the study showed that the Actinobacillus succinogenes is the best succinate bacteria to provide commercial Succinic acid. The presence of 10 g of glucose was satisfactory in improving the fermentation among all bacteria. The highest Succinic acid production was 12.8 g/L provided by the corn fiber. The lowest succinic acid production was 18% less than the glucose medium that the orange peel gave. The results revealed a significant impact of the raw material composition on the production outcomes. At the bioreactor, the cells exhibited an exponential phase with rate growth of 0.175 [g/L.h] and the maximum Succinic acid produced was 25 g/L.
Microbial succinic acid production: Natural versus metabolic engineered producers
Process Biochemistry, 2010
The increased consciousness for environmental issues and the depletion of mineral oil reserves led to the search for alternative energy sources but also for alternative biochemical processes. One of these chemicals that is identified to have great economical potential in a biobased economy is succinic acid. This chemical is a precursor for various high value-added derivatives which have application in the detergent/surfactant market, the ion chelator market, the food market and the pharmaceutical market. This review investigates the goals ...
Production of succinic acid by bacterial fermentation
Enzyme and Microbial Technology, 2006
Succinic acid produced by various microorganisms can be used as a precursor of many industrially important chemicals in food, chemical and pharmaceutical industries. The assessment of raw material cost and the estimation of the potential market size clearly indicate that the current petroleum-based succinic acid process will be replaced by the fermentative succinic acid production system in the foreseeable future. This paper reviews processes for fermentative succinic acid production, especially focusing on the use of several promising succinic acid producers including Actinobacillus succinogenes, Anaerobiospirillum succiniciproducens, Mannheimia succiniciproducens and recombinant Escherichia coli. Processes for the recovery of succinic acid from fermentation broth are also reviewed briefly. Finally, we suggest further works required to improve the strain performance suitable for successful commercialization of fermentative succinic acid production.
Production of Succinic Acid Through Anaerobic Screening of Related Microbial Strain
2000
Fermentation derived succinic acid is an economic process for supplying the existing succinic acid especially in chemical market. Production of succinic acid by fermentation can generate significant new markets for agricultural carbohydrates. The present work was undertaken with the objective to investigate a novel and simple fermentation process of succinic acid production at low cost from the renewable sources. Out of one hundred and two DBRL; isolates which are used in the study, only five strains gives positive results under both aerobic and anaerobic condition for succinic acid production. When these one hundred and two isolates were tested in indicator medium without CaCO only nineteen strains gives positive results under anaerobic 3 condition. However, only eighteen strains showed positive results with CaCO under same condition and only 3 five strains showed positive results with and without CaCO under anaerobic condition. From the present study 3
Chiang Mai University Journal of Natural Sciences, 2016
This study used Actinobacillus succinogenes DSMZ 22257 to produce succinic acid using sorghum straw hydrolysate (SSH) as a low-cost carbon source. In anaerobic fermentation, the maximum succinic acid concentration of 52.180 g/l, corresponding to a yield of 0.870 g/g glucose was obtained from 60 g/l of glucose and faster cells growth was also observed. When using 40 g/l of SSH as a carbon source, succinic acid of 16.671 g/l, corresponding to yield of 0.777 g/g substrate was achieved after 24 h of cultivation. Statistical method: Plackett-Burman Design (PBD) was applied for a preliminary optimization of succinic acid fermentation medium by A. succinogenes DSMZ 22257. The highest succinic acid of 15.746 g/l was obtained with fermentation medium contained 50.0 g/l of yeast extract, 5.0 g/l of urea, 5.0 g/l of CaCl 2 , 0.25 g/l of MnCl 2 , 2.50 g/l of Na 2 CO 3 and 50 g/l of MgCO 3. The results from PBD, yeast extract and MgCO 3 were identified as the key medium components. The present study suggested that the renewable sorghum straw could be utilized as an alternative carbon source for succinic acid production. Further studies of the key medium components will be optimized using Response Surface Methodology to obtain optimum succinic acid production.
Bioresource technology, 2006
The most influential parameters for succinic acid production obtained through one at a time method were sucrose, tryptone, magnesium carbonate, inoculum size and incubation period. These resulted in the production of 7.0 g L À1 of succinic acid in 60 h from Escherichia coli W3110 under anaerobic conditions. Based on these results, a statistical method, face centered central composite design (FCCCD) falling under response surface method (RSM) was employed for further enhancing the succinic acid production and to monitor the interactive effect of these parameters, which resulted in a twofold increase in yield (14.3 g L À1 in 48 h). The analysis of variance (ANOVA) showed the adequacy of the model and the verification experiments confirmed its validity. On subsequent scale-up in a 10-L bioreactor using conditions optimized through RSM, 24.2 g L À1 of succinic acid was obtained in 30 h. This clearly indicated that the model stood valid even on large-scale. Thus, the statistical optimization strategy led to a 3.5-fold increase in the yield of succinic acid. This is the first report on the use of FCCCD to improve succinic acid production from E. coli.
Well Knowledge of the Physiology of Actinobacillus succinogenes to Improve Succinic Acid Production
Applied Microbiology
The anaerobic fermentation of glucose and fructose was performed by Actinobacillus succinogenes 130Z in batch mode using three different volume of bioreactors (0.25, 1 and 3 L). The strategy used was the addition of MgCO3 and fumaric acid (FA) as mineral carbon and the precursor of succinic acid, respectively, in the culture media. Kinetics and yields of succinic acid (SA) production in the presence of sugars in a relevant synthetic medium were investigated. Work on the bench scale (3 L) showed the best results when compared to the small anaerobic reactor’s succinic acid yield and productivity after 96 h of fermentation. For an equal mixture of glucose and fructose used as substrate at 0.4 mol L−1 with the addition of FA as enhancer and under proven optimal conditions (pH 6.8, T = 37 °C, anaerobic condition and 1% v/v of biomass), about 0.5 mol L−1 of SA was obtained, while the theoretical production of succinic acid was 0.74 mol L−1. This concentration corresponded to an experiment...