Study of Oxidant (MDA) and Antioxidants (SOD & Vitamin E) in Hypertensive Patients and Normotensive Individuals (original) (raw)
Related papers
Antioxidant Status, Oxidative Stress and Lipid Profile in Essential Hypertensive Men
Journal of Evolution of Medical and Dental Sciences, 2013
Hypertension is a major health burden and coexists of with imbalance in antioxidants and lipid profile. This study is done to evaluate the total antioxidant status and oxidative stress like Malondialdehyde (MDA), catalase, superoxide dismutase (SOD), uric acid and correlate with lipid profile among hypertensive men. Significant increase in MDA, uric acid and dyslipidemia was found among hypertensive men. Also total antioxidant status and other antioxidants like SOD, catalase was found to be decreased in hypertensive men. Hence it is concluded that lipid peroxidation occur in hypertension which leads to endothelial dysfunction, renal blood flow alteration and tissue damage. As a result of which dyslipidemia, hyperuricemia and decrease in anti oxidants are seen. Hence decrease in antioxidants is a useful marker for antioxidant therapy to prevent organ damage among hypertensive.
Lipid peroxidation the levels of antioxidant enzymes in hypertension
Free Radicals and Antioxidants, 2012
There is substantial evidence that oxidative stress plays a major role in hypertension and subsequently the atherosclerotic process. The aim of the present study was to investigate the presence of oxidative stress in hypertension. Materials and Methods: The study included 46 hypertensive and 48 control subjects. Superoxide dismutase, glutathione, glutathione peroxidase activity and malondialdehyde level were measured in serum samples of the participants along with fasting lipid levels. Statistical Analysis Used: All data was entered into an Excel spreadsheet and analyzed using standard statistical software like Statistical Package for the Social Sciences (SPSS). Chi square test was used for categorical variables. Results: Serum malondialdehyde level was significantly raised in the study group as compared to control group (p < 0.05). Superoxide dismutase activity, whole blood glutathione levels and glutathione peroxidase activity were significantly decreased in all the subgroups of study group as compared to control group (p < 0.05). Cholesterol, low density lipoproteins and triglycerides showed significant rise, whereas high density lipoprotein was decreased as compared to normal. Conclusions: The higher malondialdehyde level and lower activity levels of other antioxidant molecules measured in this study could have resulted from increased free radical generation, which may confirm the presence of oxidative stress in hypertension. However, further elaborate clinical studies are required to evaluate the role of such antioxidant enzymes.
Oxidant–antioxidant Status and Lipid Profile in the Hypertensive Patients
Journal of Nepal Health Research Council, 2009
Background: Hypertension is associated with an elevation of ROS and frequently also with an impairment of endogenous antioxidant mechanisms. Increased level of serum cholesterol, TG, VLDL has been observed in patients with hypertension. It has been shown that oxidized lipoprotein inactivates NO and aggravates hypertension. This study intends to know the association of oxidative stress and lipid profile with hypertensive patients of the Western Nepal.
Association of low anti oxidant status with hypertension: cause or consequence
IOSR Journal of Pharmacy and Biological Sciences, 2012
Background and objectives: Recent studies imply an increasing association of oxidative stress in the pathogenesis of hypertension. This study was designed to evaluate oxidative stress in hypertensive subjects and to assess the correlation of antioxidant status with the severity of hypertension. Material and methods: Ninety-six hypertensive cases were divided into three groups based on severity of hypertension as per JNC VII classification. Lipid profile, antioxidant power of serum(FRAP assay) and oxidant load of serum(FOX2) of hypertensive cases were compared with an equal number of age and sex matched healthy normotensive controls. Data was analysed by Student's t test and Pearson's correlation. Result: Lipid profile, oxidant load was significantly higher in hypertensive patients in comparison to controls (P<0.001). Antioxidant status was lower in hypertensives as compared to controls. A significant correlation was observed between lipid profile vs. oxidative stress and hypertension vs. low antioxidant level (r=0.835, P<0.001) Conclusion: Above findings demonstrate a strong association between blood pressure, dyslipidaemia and oxidative parameters shows a possible role of oxidative stress in the severity of hypertension.
A clinical study showing altered antioxidants profile in patients with hypertension
Pakistan journal of pharmaceutical sciences, 2018
We compared the plasma antioxidants level of normal control group with that of hypertensive patients in order to test the hypothesis "that antioxidants level has been diminished in hypertensive patients and that antioxidants are interconnected with each other making a network. The plasma and red blood cells antioxidants level of newly diagnosed hypertensive patients [(n=30), (mean age 53 years), (mean systolic BP 158 mmHg, mean diastolic BP 100 mmHg)] were compared to those of the control subjects [(n=30), (mean age 50 years), (mean systolic BP 126 mmHg, mean diastolic BP 90 mmHg)] using liquid chromatography linked with electrochemical detector (HPLC-ECD). The data was analyzed by Minitab software at a 95% confidence interval (p<0.05) as significant. The comparison between the two groups was made applying 2-sample and paired t-test. The individual concentration of antioxidants in both plasma and red blood cells of hypertensive patients was lower in comparison with that of c...
Relationship between Oxidative Stress and Essential Hypertension
Hypertension Research, 2007
This study investigated the association of blood pressure with blood oxidative stress-related parameters in normotensive and hypertensive subjects. A cross-sectional design was applied to 31 hypertensive patients and 35 healthy normotensive subjects. All subjects were men between the ages of 35 and 60 years. Exclusion criteria were obesity, dyslipidemia, diabetes mellitus, smoking and current use of any medication. All patients underwent 24-h ambulatory blood pressure monitoring and sampling of blood and urine. Antioxidant enzymes activity, reduced/oxidized glutathione ratio (GSH/GSSG), and lipid peroxidation (malondialdehyde) were determined in erythrocytes. Parameters measured in the plasma of test subjects were plasma antioxidant status, lipid peroxidation (8-isoprostane), plasma vitamin C and E, and the blood pressure modulators renin, aldosterone, endothelin-1 and homocysteine. Daytime systolic and diastolic blood pressures of hypertensives were negatively correlated with plasma antioxidant capacity (r =-0.46, p <0.009 and r =-0.48, p <0.007), plasma vitamin C levels (r =-0.53, p <0.003 and r =-0.44, p <0.02), erythrocyte activity of antioxidant enzymes, and erythrocyte GSH/GSSG ratio, with hypertensives showing higher levels of oxidative stress. Blood pressures showed a positive correlation with both plasma and urine 8-isoprostane. Neither plasma vitamin E nor the assessed blood pressure modulator levels showed significant differences between the groups or correlation with blood pressures. These findings demonstrate a strong association between blood pressure and some oxidative stress-related parameters and suggest a possible role of oxidative stress in the pathophysiology of essential hypertension. (Hypertens Res 2007; 30: 1159-1167)
Study of Oxidative Stress in Essential Hypertension
Biomedical and Pharmacology Journal, 2015
Essential hypertension is associated with an elevation of reactive oxygen species (ROS) which reacts with membrane lipids to form lipid hydroperoxides that decomposes to form Malondialdehyde (MDA) an indicator of oxidative stress. Endogenous antioxidant enzyme, Superoxide dismutase (SOD) counteracts oxidative stress. This study aims at understanding the role of oxidative stress in essential hypertension. The study comprised of 50 confirmed cases of hypertension and 50 age and sex matched controls .Inclusion criteria includes patients with blood pressure e" 140/90 mm of Hg, while patients with secondary hypertension, stroke, CAD,MI and diabetes mellitus are excluded. Serum SOD estimated spectrophotometrically by Mishra H.P. & Fridovich I,1972 method and Plasma MDA by colorimetric method of Satoch K.et al. SOD activity was statistically significantly (p<0.0001) decreased while MDA level was statistically significantly (p<0.0001) increased in hypertensives compared to controls. Patients suffering from hypertension have increased ROS activity which oxidizes nitric oxide (NO) and affect vascular tone.Lassegue et al (2004) also found convincing evidence that ROS is an intrinsic part of pathology of hypertension. If oxidative stress is indeed a cause or consequence of hypertension, then reduction in oxidative damage may result in a reduction in blood pressure. Antioxidants like Vit.A,Vit.C & Vit E which are present in vegetables, citrus fruits & oils respectively are able to trap ROS and thus may be capable of reducing oxidative damage and possibly blood pressure. Estimation of oxidative stress markers (SOD & MDA) is simple and inexpensive; it can be used to predict the development of atherosclerotic disease like coronary artery disease, cerebrovascular disease and renal complications associated with essential hypertension. Oxidative stress markers estimation may also be helpful in assessing the usefulness of antihypertensive drugs in prevention of associated complications.
Analysis Of Level Of Antioxidants In The Prognosis Of Hypertension ., Indo Am
The main objective of the study is to find the level of antioxidants in case of hypertension patients because hypertension directly effect on blood level and heart of the patient. Methodology of the study: This cross sectional study was conducted in Tehsil Headquarter Hospital Khairpur Tamewali Bahawalpur during January 2019 to August 2019. All the data was collected according to the rules and regulations of authority. The data was collected from both genders of age between 30 to 50years. The blood was drawn from all patients for further analysis of antioxidants. Results: Our results showed that the level of antioxidants increases in hypertension patients due to increase in blood flow. The level of MDA, SOD, GSH and CAT vary in a different manner. The level of SOD become decreases due to hypertension. Antioxidants are compounds that are able to trap ROS and thus may be capable of reducing oxidative damage and possibly blood pressure. Conclusion: In conclusion, we found that hypertension increased free radical levels in the blood. According to our study, levels of free radicals increase in the blood, which may stimulate antioxidant defense systems of body during hypertension.
2020
Background: Hypertension is recognized as most common cardiovascular disorder and a leading cause of morbidity and mortality worldwide. There is abnormal lipid peroxidation which suggested that oxidative stress is important in the pathogenesis of hypertension. This study aims at understanding the role of oxidative stress in hypertension. Aim of the Work: The objective of this study was to determine the malondialdehyde (MDA) in patients with hypertension and healthy controls. Subjects and Methods: Whole study population included 250 hypertensive cases and 250 controls. All individuals included in the study were submitted to: Complete history and physical examinations to evaluate exclusion criteria. Serum MDA was estimated by method of Jean et al using trichloroacetic acid and thiobarbituric acid. Mean and standard deviation were calculated for serum MDA. Statistical analysis was done using SPSS no. 20 and Microsoft excel. Result: There was statistically higher significant increase (...
Clinical Science, 2008
Oxidative stress has been associated with mechanisms of EH (essential hypertension). The aim of the present study was to test the hypothesis that the antioxidant properties of vitamins C and E are associated with a decrease in BP (blood pressure) in patients with EH. A randomized double-blind placebo-controlled clinical trial was conducted in 110 men with grade 1 EH (35–60 years of age without obesity, dyslipidaemia and diabetes mellitus, non-smokers, not undergoing vigorous physical exercise, without the use of any medication and/or high consumption of fruit and vegetables). Participants were randomly assigned to receive either vitamins C+E [vitamin C (1 g/day) plus vitamin E (400 international units/day)] or placebo for 8 weeks. Measurements included 24 h ambulatory BP and blood analysis of oxidative-stress-related parameters in erythrocytes (GSH/GSSH ratio, antioxidant enzymes and malondialdehyde) and plasma [FRAP (ferric reducing ability of plasma)], and levels of 8-isoprostane,...