Anti-adherent and antifungal activity of synthetic and organic adhesive polymers on >i/i biofilms (original) (raw)
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Longitudinal Evaluation of Ethyl Cyanoacrylate Adhesives on Candida albicans Biofilm
International journal of odontostomatology
The aim of this study is to evaluate the effect of two ethyl cyanoacrylate-based adhesives on the growth of Candida albicans biofilms on a heat-polymerized resin, after 7, 14, and 30 days of exposure. Ninety circular (10 × 2 mm) heat-polymerized resin specimens were equally divided into three groups: control, conventional ethyl cyanoacrylate (ECAc), and ethyl cyanoacrylate gel (ECAg). Two layers of 50 µL of each material were applied to the respective groups. C. albicans SC5314 strain was activated and standardized to 10 7 cells/mL-1. Specimens were immersed in 1 mL of artificial saliva and deposited in 1 mL fungal suspension, washed, and immersed in 1 mL of RPMI for 7, 14, and 30 days. The medium was changed at 48-hour intervals. The final suspension was diluted (10-1 to 10-4) and deposited on Sabouraud dextrose agar for 48 h at 37 °C. After this period, the colonies were quantified using the CFU/mL calculation. Data were evaluated using oneway ANOVA and Tukey's test for post-hoc analysis (P=0.05). It was observed that both adhesives significantly reduced (P<0.05) biofilm formation compared to the control at all evaluated periods. In conclusion, an immediate and long-term inhibitory effect on C. albicans biofilm formation was observed.
Polymers, 2022
Denture stomatitis is a common oral infection caused by Candid albicans. It occurs under removable dentures due to several causative and contributing factors. If not treated, it can lead to fatal systemic candida infections. Different materials and techniques have been used to treat denture stomatitis; however, no single treatment has succeeded. The purpose of this study was to review novel techniques for incorporating antimicrobial and protein repellent agents into denture acrylic resin materials and their effect on the adhesion of Candida albicans to denture base acrylic resin materials to prevent and/or treat denture stomatitis. A systematic review was conducted in which an electronic search was undertaken using different databases and relevant keywords. The literature search revealed numerous studies describing different antifungal materials incorporated into different denture acrylic resin materials. The investigated materials showed significant antimicrobial activity with slig...
Poly(methyl methacrylate) with Oleic Acid as an Efficient Candida albicans Biofilm Repellent
Materials
Poly(methyl methacrylate) (PMMA), widely used in dentistry, is unfortunately a suitable substrate for Candida (C.) albicans colonization and biofilm formation. The key step for biofilm formation is C. albicans ability to transit from yeast to hypha (filamentation). Since oleic acid (OA), a natural compound, prevents filamentation, we modified PMMA with OA aiming the antifungal PMMA_OA materials. Physico-chemical properties of the novel PMMA_OA composites obtained by incorporation of 3%, 6%, 9%, and 12% OA into PMMA were characterized by Fourier-transform infrared spectroscopy and water contact angle measurement. To test antifungal activity, PMMA_OA composites were incubated with C. albicans and the metabolic activity of both biofilm and planktonic cells was measured with a XTT test, 0 and 6 days after composites preparation. The effect of OA on C. albicans morphology was observed after 24 h and 48 h incubation in agar loaded with 0.0125% and 0.4% OA. The results show that increase o...
Biomedical and Pharmacology Journal, 2018
The aim of this study was to evaluate the effect of modified heat cured polymethyl methacrylate denture base (PMMA) or varnish coating of the denture base on candida albicans adhesion and porosity. First experimental group was prepared by addition of 15% phosphoric acid 2-hydroxyethyl methacrylate ester with polymethyl methacrylate monomer. While second experimental group prepared by varnish layer coating after material processing, these groups were compared with the control one. Three groups of vertex acrylic resin used as control, mPMMA and varnish coated group (PPH Cerkamed Wojciech Pawlowski, Poland). The inverted light microscope was used to measure the candida albicans adherence after one week and one month of processing. Two-way ANOVA, LSD test, T- test and Dunnett T3 were performed on data. Modified Acrylic resin showed highly significant reduction in candida albicans adhesion and porosity, also for varnish coating group showed highly significant reduction in candida albican...
Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology, 2012
The aim of this study was to evaluate in vitro the preventive antiadhesion activity of biosurfactants against Candida albicans biofilm. Study Design. Disks of silicon and acrylic resin for denture prostheses were precoated with increasing concentrations of biosurfactants obtained from endophyte biofilms selected from Robinia pseudoacacia and from Nerium oleander, and afterward infected with C. albicans cells. The number of biofilm cells were detected by colony-forming unit (CFU) counting, cell viability was established by the 2,3-bis(2-methoxy-4-nitro-5-sulphophenyl)-5-[(phenyl amino)carbonyl]-2H-tetrazolium hydroxide (XTT) assay, and biosurfactant cytotoxicity was evaluated by the [3-(4,5-dimethyliazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulphophenyl)-2H-tetrazolium] (MTT) assay. Chlorhexidine was used as control. Results. Precoating with biosurfactants caused a greater reduction (P Ͻ .01) in biofilm cell number and viability than chlorhexidine. The antiadhesion activity of the biosurfactants was observed at low concentrations (78.12 g/mL and 156.12 g/mL) which were noncytotoxic. Conclusions. This study demonstrated the preventive antiadhesion activity of biosurfactants against C. albicans biofilm. These agents are amphiphilic, interfere with microbial adhesion, and demonstrate cycompatibility with epithelial cells and fibroblasts.
Journal of Materials Science: Materials in Medicine, 2018
The aim of this study is to evaluate the effects of 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer coating with various concentrations onto acrylic resin denture base material on surface characteristics such as contact angle and surface roughness and on Candida albicans adhesion which is the major factor of denture stomatitis. Specimens, prepared from heatpolymerized acrylic denture base material, were divided into control and three test groups, randomly. Surfaces of the specimens in test groups were coated with poly(MPC) (PMPC) by graft polymerization of MPC in different concentrations (0.25 mol/L; 0.50 mol/L and 0.75 mol/L), while no surface treatment was applied to the control group. Contact angles and surface roughness were examined, and chemical composition of the surfaces was analyzed by Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopy (FTIR) to verify the presence of PMPC coatings. Then, specimens were incubated with C. albicans for 18 h and the number of adhered cells was determined. Upon PMPC coating, the contact angle values statistically decreased, but no difference was found in surface roughness values. A statistically significant decrease was observed in C. albicans adhesion in parallel with the increase in the MPC polymer concentration. There was no significant difference between 0.50 mol/L and 0.75 mol/L groups in terms of adhesion. These findings indicated that graft polymerization of MPC on acrylic denture base material reduces the adhesion of C. albicans, and may be evaluated as a coating for prevention of denture stomatitis.
Medical principles and practice : international journal of the Kuwait University, Health Science Centre, 2015
We aimed to investigate the effect of brief exposure to sub-cidal concentrations of nystatin, amphotericin B, ketoconazole, fluconazole and chlorhexidine gluconate on the adhesion of oral Candida dubliniensis isolates to the surface of acrylic dentures. After determining the minimum inhibitory concentration of each drug, 20 oral isolates of C. dubliniensis were exposed to sub-cidal concentrations of the drugs for 1 h. The drugs were then removed by dilution, and the adhesion of the isolates to denture acrylic strips was assessed by an in vitro adhesion assay. Compared to the controls, exposure to nystatin, amphotericin B, ketoconazole, fluconazole and chlorhexidine gluconate suppressed the ability of C. dubliniensis isolates to adhere to acrylic denture surfaces with a reduction of 74.68, 74.27, 57.31, 44.57 and 56.53% (p < 0.001 for all drugs), respectively. Brief exposure to sub-cidal concentrations of anti-mycotics suppressed the adhesion of C. dubliniensis oral isolates to ac...
2020
Background: PTFE is an anti-adherent and aesthetic material. The aim of this study was to evaluate, in vitro, the influence of PTFE coating on the biofilm formation between Polytetrafluoroethylene (PTFE) coated denture resin surfaces and uncoated denture resin surfaces. Materials and Methods: Two groups were tested [Group 1: control, pure polymethylmethacrylate(PMMA); Group 2: modified PMMA with PTFE coating. Thirty resin specimens for each group were polymerized, and four experimental subgroups for each surface type were devised, consisting of 2 and 4 days of incubation in C. albicans suspension. After incubation one set for sample was taken for colony counting whereas other set was used for crystal violet staining and UV spectrophotometer assay. Results: At day 2, the PTFE coated resin had statistically significantly lower levels of Candida than the uncoated group. The PTFE coated resin had statistically significantly lower levels of Candida accumulation at days 2 and 4 compared t...
Ellepola, A. N., B. K. Joseph, et al. (2014). "In vitro Adhesion of Oral Candida dubliniensis Isolates to Acrylic Denture Surfaces following Brief Exposure to Sub-Cidal Concentrations of Polyenes, Azoles and Chlorhexidine." Med Princ Pract. Objectives: We aimed to investigate the effect of brief exposure to sub-cidal concentrations of nystatin, amphotericin B, ketoconazole, fluconazole and chlorhexidine gluconate on the adhesion of oral Candida dubliniensis isolates to the surface of acrylic dentures. Methods: After determining the minimum inhibitory concentration of each drug, 20 oral isolates of C. dubliniensis were exposed to sub-cidal concentrations of the drugs for 1 h. The drugs were then removed by dilution, and the adhesion of the isolates to denture acrylic strips was assessed by an in vitro adhesion assay. Results: Compared to the controls, exposure to nystatin, amphotericin B, ketoconazole, fluconazole and chlorhexidine gluconate suppressed the ability of C. dubliniensis isolates to adhere to acrylic denture surfaces with a reduction of 74.68, 74.27, 57.31, 44.57 and 56.53% (p < 0.001 for all drugs), respectively. Conclusions: Brief exposure to sub-cidal concentrations of anti-mycotics suppressed the adhesion of C. dubliniensis oral isolates to acrylic denture surfaces. (c) 2014 S. Karger AG, Basel.
Journal of Advanced Oral Research
The purpose of this study was to understand the complexities of Candida albicans biofilm formation and to evaluate the effectiveness of most used antimicrobial agents. Materials and Methods: About 24 acrylic resin specimens (2 mm thickness, 10 mm diameter) were prepared. The contact angle and surface roughness of all specimens were measured. C. albicans ATCC10231 was incubated in Sabouraud dextrose agar (SDA) at 37°C for 24 h. Subsequently, SDA was incubated at 37°C for 48 h. Specimens were immersed to 1% and 2% sodium hypochlorite and phosphate-buffered saline for 10 min and 4% chlorhexidine gluconate for 5 min. The efficiency of chemical disinfectants in removing C. albicans biofilm was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium-bromide assay test. After application of chemical disinfectant, acrylic resin specimens were examined using scanning electron microscopy (SEM). The evaluation of the efficiency of chemical disinfectant was analyzed with one-way ANOVA and post-hoc Tukey test. Results: The highest and lowest efficiency to remove C. albicans biofilm was identified for 1% sodium hypochlorite and 4% chlorhexidine gluconate. The efficiency of 1% and 2% sodium hypochlorite solution to remove biofilm showed no statistical difference. SEM analysis indicated that after the application of 1% and 2% sodium hypochlorite no fungal cells were observed, and application of 4% chlorhexidine gluconate revealed a few remaining blastospores and hyphal cells attached to the resin surfaces. Conclusions: Disinfection with 1% and 2% sodium hypochlorite solutions revealed the most impressive results to reduce the C. albicans biofilm formation than 4% chlorhexidine gluconate solution.