The Effects of Methanolic Extract of Prosopis Farcta Seed on Blood Glucose in Streptozocin Induced Diabetic Rats (original) (raw)
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Advanced Biomedical Research, 2016
Background: The use of herbals in the treatment of diabetes mellitus is a well-established practice in traditional medicine. The medicinal plant Prosopis farcta has some antioxidant activity, which may be useful in diabetic patients. Since, there is no report on the antidiabetic effect of the P. farcta, this study evaluated antidiabetic activity of P. farcta bean extract (PFE) in streptozotocin (STZ)-induced diabetic rats. Materials and Methods: Hyperglycemia was induced in male albino Wistar rats by intraperitoneal injection of STZ (55 mg/kg body weight [BW]), after which, the animals were randomly allocated into six experimental groups as follows: Group 1: Normal rats (received normal saline), Groups 2 and 3: Normal rats received PFE; (50 and 75 mg/kg BW), Group 4: Diabetic control rats, Group 5: Diabetic rats received PFE (50 mg/kg BW), Group 6: Diabetic rats received PFE (75 mg/kg BW). Three days after induction of diabetes, rats were received an extract of PFE orally for 12 days. Blood samples were collected by cardiac puncture to determine liver enzymes; aspartate aminotransferase and alanine aminotransferase (AST and ALT), cholesterol, triglyceride (TG), high and low density lipoproteins (HDL and LDL). Results: The administration of PFE (50 and 75 mg/kg) in STZ-induced diabetic rats significantly reduced the blood glucose levels when compared with the STZ-control group (227.2 ± 12.00 and 259.6 ± 7.03 vs. 454.6 ± 12.66, P < 0.001). PFE in diabetic groups had no significant effect on the levels of cholesterol, TG, HDL, LDL, AST, and ALT compare to the STZ-control group. Conclusion: P. farcta could reduce blood glucose in diabetic rats.
Zahedan Journal of Research in Medical Sciences
Background and Objective: Prosopis farcta fruit (PFF) is known to have antioxidant activity. Antioxidants can reduce and prevent dyslipidemia and hyperglycemic in diabetic patients. This study was conducted to investigate the effect of PFF hydroalcoholic extract on some blood biochemical parameters in insulin resistance model of rats. Methods: In this experimental study, diabetes was induced by feeding the animals with fructose (12% w/v) and soybean (20% dry matter) for 6 weeks followed by a single intraperitoneal injection of streptozotocin (30 mg/kg). The animals were divided into four groups: 1 and 2) Healthy and diabetic controls; 3 and 4) healthy and diabetic rats receiving PFF (100 mg/kg body weight). The blood samples were collected and the serum concentrations of glucose, triglycerides, cholesterol, HDL-C, LDL-C and VLDL-C were investigated. Results: Streptozotocin increased serum levels of glucose, triglycerides, cholesterol and LDL-C (P < 0.05), but PFF extract lowered the serum concentrations of these variables (P < 0.05) in diabetic rats. Conclusions: PFF extract might be useful for the treatment of hyperglycemic and hyperlipidemic in diabetic patients.
Evidence-Based Complementary and Alternative Medicine, 2020
By using the streptozotocin- (STZ-) induced cytotoxicity in β-TC3 cells as an assay model, a bioassay-guided fractionation study was employed to isolate and characterize the potential antidiabetic principles of roots of Prosopis farcta. A combination of open column chromatography on reverse-phase silica gel using a water-ethanol gradient (10 : 90 to 100 : 0) followed by HPLC-based fractionation led to an active compound that appears to be composed of carbohydrate/sugar. When cell viability under STZ was reduced to 49.8 ± 4% (mean ± SD), treatment with the active compound at the concentration of 0.5 mg/mL either as a coadministration or a pretreatment improved the viability to 93 ± 1.9% and 91.5 ± 7%, respectively. The reduction in the mitochondrial membrane potential by STZ (47.34 ± 8.9% of control) was similarly recovered to 84.5 ± 4.3 (coadministration) and 88 ± 5.5% (pretreatment) by the active fraction. The bioassay-guided fractionation, β-cell protective effect, and increased g...
Prosopis ruscifolia, vinal, is used to treat diabetes. This work aims to study the influence of the hydro-alcoholic extract of this plant on alloxan induced diabetic rats. Hydroalcoholic extract from aerial parts of Prosopis ruscifolia Griseb. (Fabaceae) was prepared (Pr) and the safety was assessed to determine the acute toxicity in mice. The hypoglycemic activity of the extract was evaluated in normo-and hyperglycemic rats. Hyperglycemia was induced by intravenous administration of alloxan monohydrate (32 mg/Kg body weight). Rats with blood glucose level higher than 200 mg/dL were used for the experiment. The animals were assigned to different groups and treated with a single dose of solvent (water, p.o.), Pr (100 mg/Kg, p.o.), tolbutamide (100 mg/Kg, p.o.) or insulin (5 IU/kg, i.p.); Pr extract was also administered to normoglycemic rats (100 mg/kg, p.o.). Fasted blood glucose level was measured at times 0, 1, 2, 4 and 24 h after treatment, in the acute test, and at days 0, 14, 21 and 28, in the chronic study. No evidence of acute toxicity in mice was observed. The results show that Pr extract significantly reduces blood glucose level in hyperglycemic rats (p < 0.01) 24 h after administration of a single oral dose of 100 mg/Kg. Treatment with Pr during 28 days showed a reduction in blood glucose level in experimentally hyperglycemic rats. Additionally, rats treated with the extract showed a reduced body weight gain. Prosopis ruscifolia hydroalcoholic extract showed low toxicity. After acute and chronic oral treatment was effective to reduce fasted blood glucose level, and the body weight gain was less after 28 days in the treated group.
Archives of Physiology and Biochemistry, 2020
The aim of study was to investigate the antidiabetic and antioxidant properties of extracts obtained from dried Prosopis farcta fruit and seeds against streptozotocin-induced diabetes in rats. According to the results, glucose, haemoglobin A1c, a-glycosidase activity, liver and kidney damage biomarkers, and malondialdehyde contents of all of the diabetic groups were found to have increased significantly according to the control group. Furthermore, the insulin and C-peptide secretions increased, and liver malondialdehyde level decreased, which were determined as the result of fluctuations in the antioxidant enzyme activities with a dose of 400 mg/kg fruit extract, while seed extract dosages of 100 and 400 mg/kg caused an increase in hepatic demage biomarkers. It was concluded that fruit extract may have insulin secretion stimulating and lipid peroxidation inhibitory effects, whereas seed extract might have caused hepatocyte damage changes to the transport functions and membrane permeability of these cells, thus causing enzymes to leak.
Vitae
Background: Diabetes mellitus treatment is based on oral hypoglycemic agents or insulin. Medicinal plants constitute an option, and the leaves of Prosopis ruscifolia (Pr) were shown to be effective in reducing glycemia in hyperglycemic animals. Objective: In this paper, we report the effect of P. rusciofolia (Pr) on insulin and incretin secretion in alloxan-induced hyperglycemic rats. Methodology: The effective dose was selected, and four groups (n=10) of Wistar rats were used. Two groups with normal glycemia received water or Pr (75 mg/Kg, per os, p.o.), and two groups with hyperglycemia induced by alloxan (intraperitoneal, ip), received water or Pr (75 mg/Kg, p.o.) for 2 weeks. Oral glucose tolerance test, and incretin and insulin levels were measured at the end of the experimental period. Results: The results showed that extract promotes better tolerance to oral glucose overload, in addition to a statistically significant (p<0.001) increase in blood levels of incretin and insu...
Journal of Ethnopharmacology, 2011
Ethnopharmacological relevance: Diabetes mellitus is rampantly increasing and the need for therapeutics is crucial. In recognition of this, untested antidiabetic agents are flooding the market. Diavite TM which is a product consisting solely of the dried and ground pods of Prosopis glandulosa (Torr.) [Fabaceae] is currently marketed as a food supplement with glucose stabilizing properties. However, these are anecdotal claims lacking scientific evidence. The aim of this study was to determine the efficacy of Prosopis glandulosa as an antidiabetic agent. Materials and methods: Male Wistar rats were rendered (a) type 1 diabetic after an intraperitoneal injection of STZ (40 mg/kg) and (b) insulin resistant after a 16-week high caloric diet (DIO). Zucker fa/fa ZDF rats were used in a pilot study. Half of each group of animals was placed on Prosopis glandulosa treatment (100 mg/kg/day) for 8 weeks and the remaining animals served as age-matched controls. At the time of sacrifice, blood was collected for glucose and insulin level determination, the pancreata of the STZ rats were harvested for histological analysis and cardiomyocytes prepared from the DIO and Zucker fa/fa hearts for determination of insulin sensitivity. Results: Type 1 diabetic model: Prosopis glandulosa treatment resulted in significant increased insulin levels (p < 0.001), which was accompanied by a significant decrease in blood glucose levels (p < 0.05). Additionally, Prosopis glandulosa treatment resulted in increased small -cells (p < 0.001) in the pancreata. The body weight of the STZ animals decreased significantly after STZ injection, with Prosopis glandulosa treatment partially preventing this. Zucker fa/fa rats: Prosopis glandulosa treatment significantly reduced fasting glucose levels (p < 0.01) and improved IPGTT, when comparing treated to untreated animals. DIO insulin resistant model: Prosopis glandulosa treatment resulted in an increased basal (p < 0.01) and insulinstimulated (p < 0.05) glucose uptake by cardiomyocytes prepared from this group. Conclusions: The present study showed that Prosopis glandulosa treatment moderately lowers glucose levels in different animal models of diabetes, stimulates insulin secretion, leads to the formation of small -cells and improves insulin sensitivity of isolated cardiomyocytes.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2018
A rapidly increasing incidence of Diabetes mellitus throughout the world is a major concern in both developed and developing countries and the drawbacks associated with currently available treatments led to switching researcher's attention towards naturopathy. Since ancient time, herbal plants have been traditionally used for the treatment of diabetes as they consider to be less toxic and free from side effects than synthetic ones. In our previous studies, we had isolated two new compounds (Methyl 5-tridecyloctadec-4-enoate and Nonacosan-8-one), together with three known compounds (Lupeol, β-sitosterol and Stigmasterol) from chloroform fraction of stem bark of P. cineraria (CfPc). The present study aimed to determine the in vivo and in vivo antidiabetic activity of CfPc in streptozotocin induced experimental diabetes and also evaluated their possible mode of action. CfPc was orally administrated to STZ (55 mg/kg b.wt) induced diabetic rats at the doses of 50 and 100 mg/kg b.wt f...
Journal of Applied Biology & Biotechnology
Diabetes is a serious chronic pathology, with long-term effects including damage to blood vessels or diabetic dyslipidemia. Diabetic dyslipidemia is characterized by increasing concentrations of low-density triglycerides and lipoproteins and a decrease in high-density lipoproteins HDL-cholesterol (HDL-c). This study aimed to evaluate the effect of Prosopis ruscifolia on lipid profile in albino Swiss mice with hyperglycemia and hyperlipidemia. Hyperglycemia was induced by alloxan and the animals were orally treated with Pr (50, 100, and 200 mg/kg) for 45 days. Hyperlipidemia was induced with tyloxapol and the animals were treated with Pr (50, 100, and 200 mg/kg). In hyperglycemic animals treated with 100 mg/kg, there was a decrease in the concentration of cholesterol, a decrease in the concentration of triglycerides, and an increase in HDL-c at the end of treatment compared to untreated hyperglycemic animals. In mice with hyperlipidemia treated with 50 and 100 mg/kg of Pr, serum cholesterol and triglyceride concentration were reduced. HDL-c increased in animals treated with Pr 50, 100, and 200 mg/kg compared to untreated animals. It was observed that the administration of P. ruscifolia in hyperglycemic and hyperlipidemic animals had a favorable effect on the lipid profile.
Quarterly of Horizon of Medical Sciences
Aims: Diabetes is a common endocrine disorder that can lead to hyperglycemia and hyperlipidemia. The aim of this study was to investigate the effect of hydroalcoholic extract of pod Prosopis fracta, on liver histopathology and tissue level of malondialdehyde (MDA) in streptozotocin-induced diabetic rats. Materials & Methods: 45 male Wistar rats (200-300g) were divided into 3 groups; control, diabetic and Prosopis farcta extract treated diabetic. Type 1 diabetes was induced in by injection of streptozotocin (42mg/kg). One week after diabetes induction, Prosopis farcta extract (300mg/kg of body weight) was administered to treated diabetic group by gavage for 30 days. Hepatic histological changes were assessed with Hematoxylin-Eosin staining under light microscopy. The liver concentration of MDA was determined as thiobarbituric acid reactive substances (TBARS). The obtained data were statistically analyzed using Students T and Mann-Whitney rank sum tests. Findings: Administration of Prosopis fracta extract decreased the concentration of malondialdehyde in liver tissue of treated diabetic group in comparison to diabetic group significantly (p<0.05). Inflation and vacuolation of hepatocytes were observed with disarrangement of hepatic cords and sinusoidal narrowing. All previous signs were improved in Prosopis fracta treated group. Conclusion: Hydro-alcoholic extract of Prosopis fracta pod can reduce the level of malondialdehyde as a marker of lipid peroxidation in liver and prevent the histopathological changes of liver associated with diabetes.