Antidiabetic Effect of Extracts of Blumea Lacera DC. In Streptozotocin Induced Hyperglycemic Rats (original) (raw)
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International Journal of Pharmacy and Pharmaceutical Sciences, 2017
Objective: Present study of this research was undertaken to evaluate the antidiabetic activities of extracts of aerial parts of Blumea lacera DC. (Asteraceae) in streptozotocin (STZ) induced hyperglycemic rat. Methods: The methanol extracts (MEBL) and aqueous extract (AEBL) of B. lacera DC. were investigated in streptozotocin (STZ) induced hyperglycemic rats at a dose level of 200 and 400 mg/kg body weight, in oral glucose tolerance test (OGTT), acute and subacute antidiabetic (30 d) models keeping a parallel group of metformin (250 mg/kg body weight) as standard drug. The serum biochemical parameters, histopathology of liver and pancreas Results: Treatment with methanol extract of B. lacera (MEBL) at a dose of 200 mg/kg and 400 mg/kg body weight, significantly decrease (p<0.05) blood glucose level from 289.83±9.83 and 289.83±2.71 to 201.83±8.87 and 105.00±2.05 respectively with corresponding percentage fall of blood glucose to 30.40±1.79 and 63.78±0.59. It also improved the glycated haemoglobin (HbA1c) near to normal value, restored the lipid and biochemical level and rejuvenate beta cells of pancreas, thereby improve insulin secretion. were examined and analyzed statistically. Conclusion: The result of the present study concluded that extracts of B. lacera DC possess marked antidiabetic activity. However, methanol extract of B. lacera (MEBL) at the dose of 400 mg/kg showed significant dose dependent antidiabetic effects without any destruction and restores the structure of liver and pancreas of hyperglycemic rats.
Journal of Pharmacognosy and Phytochemistry, 2017
Three plants from indian origin like aerial parts of Schrebera swietenoides, roots of Barleria montana and aerial parts of Rotula aquatica were extracted with methanol and metanolic extracts were evaluated for antidiabetic activity against streptozotocin induced diabetes for their study. Oral administration of these plant extracts at different dose levels of 100 mg/kg, 200 mg/kg and 400 mg/kg were screened in comparision with the standard drug glibenclimide. Among these plant extracts, extract of Barleria montana at a dose of 400 mg/kg b.w exhibited significant activity within 4th and 8th hour intervals showing a reduction in blood glucose levels are 293.94± 4.63 and 235.04± 2.93 mg/dl. preliminary phytochemical screening also conducted which revealed presence of triterpenes, flavonoids and steroid
Medicinal Plants: A Methodology for Studying their Anti-Diabetic Activity
TJPRC, 2013
This work is a review on the study methods of the antidiabetic activity of medicinal plants. Indeed, this discipline is not standardized and techniques used to assess this activity are very varied. The choices of the plant and the extract to be tested are major parameters before going to the model of diabetes. The latter is induced into animals by various techniques including the injection of chemicals such as streptozotocin which damages the pancreatic β cells. Hence, diabetes develops spontaneously in certain animal species. Works realized on animals (preclinical) would permit to research on human being (clinical).
Journal of Ethnopharmacology, 2007
Sclerocarya birrea (Anacardiaceae) is used as a traditional treatment of diabetes in Cameroon. In this study, we investigated the possible antidiabetic effect of the stem bark extract in diabetic rats. Diabetes was induced by intravenous injection of streptozotocin (STZ, 55 mg/kg) to male Wistar rats. Experimental animals (six per group), were treated by oral administration of plant extract (150 and 300 mg/kg body weight) and metformin (500 mg/kg; reference drug) for comparison, during 21 days. The stem bark methanol/methylene chloride extract of Sclerocarya birrea exhibited at termination, a significant reduction in blood glucose and increased plasma insulin levels in diabetic rats. The extract also prevented body weight loss in diabetic rats. The effective dose of the plant extract (300 mg/kg) tended to reduce plasma cholesterol, triglyceride and urea levels toward the normal levels. Four days after diabetes induction, an oral glucose tolerance test (OGTT) was also performed in experimental diabetic rats. The results showed a significant improvement in glucose tolerance in rats treated with Sclerocarya birrea extract. Metformin, a known antidiabetic drug (500 mg/kg), significantly decreased the integrated area under the glucose curve. These data indicate that Sclerocarya birrea treatment may improve glucose homeostasis in STZ-induced diabetes which could be associated with stimulation of insulin secretion.
Experimental and Toxicologic Pathology, 2011
The present study evaluates the possible hypoglycemic activity and ameliorative effects of oral administration of ethanol extracts (EEBC) and butanol fraction (BFBC) of Buchholzia coriacea seeds, a plant in use traditionally for treating diabetes, hypertension, rheumatism, cold, cough and catarrh, in streptozotocin (STZ)-induced diabetic mice and rats. Fasting blood glucose (FBG) levels were evaluated before and after extracts administration. EEBC and BFBC significantly decreased (P < 0.05) FBG in hyperglycemic mice and normoglycemic rats within 4 and 12 h, respectively after extract administration. The administration of EEBC, BFBC and glibenclamide (a standard antidiabetic drug) for 10 days significantly lowered (P < 0.05) FBG level in STZ-induced diabetic rats by 55%, 64% and 56%, respectively. EEBC and BFBC significantly (P < 0.05) decreased hepatic injury induced by STZ as evident in the decreased activity of serum alanine amino transferase and aspartate amino transferase compared to in the STZ-only treated group. Similarly, both extracts significantly decreased (P < 0.05) the elevated levels of serum creatinine, urea, total cholesterol, triglyceride and thiobarbituric acid reactive species (TBARS) products in diabetic rats. Serum superoxide dismutase activity was significantly enhanced (P < 0.05) by treatments with EEBC, BFBC and glibenclamide. Overall, the results suggest that B. coriacea seeds contain a potent hypoglycemic and antioxidant agent suggested to be a flavone glycoside concentrated in BFBC which may find clinical application in amelioration of diabetes-induced secondary complications.
Chemical and Biological Aspects of Extracts from Medicinal Plants with Antidiabetic Effects
The Review of Diabetic Studies, 2016
Diabetes mellitus is a chronic disease and a leading cause of death in western countries. Despite advancements in the clinical management of the disease, it is not possible to control the late complications of diabetes. The main characteristic feature of diabetes is hyperglycemia, which reflects the deterioration in the use of glucose due to a faulty or poor response to insulin secretion. Alloxan and streptozotocin (STZ) are the chemical tools that are most commonly used to study the disease in rodents. Many plant species have been used in ethnopharmacology or to treat experimentally symptoms of this disease. When evaluated pharmacologically, most of the plants employed as antidiabetic substances have been shown to exhibit hypoglycemic and antihyperglycemic activities, and to contain chemical constituents that may be used as new antidiabetic agents. There are many substances extracted from plants that offer antidiabetic potential, whereas others may result in hypoglycemia as a side effect due to their toxicity, particularly their hepatotoxicity. In this article we present an updated overview of the studies on extracts from medicinal plants, relating the mechanisms of action by which these substances act and the natural principles of antidiabetic activity.
Indian journal of experimental biology, 2010
The present investigation was undertaken to standardize and study the dose-dependent effect of three weeks treatment with hot and cold aqueous extract of E. littorale (0.5, 1 and 2 g/kg, po) on streptozotocin (STZ) induced type I diabetic (confirmed by histopathology) rats (45 mg/kg, iv single dose). Treatment of rats with STZ produced cardinal signs of diabetes-mellitus like a significant loss of body weight, polyuria and polydipsia. There was also a significant increase in fasting blood glucose levels and AUC(glucose) associated with decrease in insulin levels and AUC(insulin) in STZ-diabetic rats. Treatment with E. littorale hot extract (1 and 2 g/kg) significantly reduced the elevated food intake and water intake, glucose and AUC(glucose) levels of diabetic rats. There was also a significant increase in serum cholesterol, serum triglyceride in the STZ diabetic rats. Treatment with E. littorale hot extract (1 and 2 g/kg) significantly decreased all these elevated levels in diabet...
Scientific Journal of Biological Sciences, 2013
The study investigated the anti-diabetic and haematological effects of n-butanol fraction of Alchornea cordifolia in streptozotocin-induced diabetic wistar rats. In this work, thirty six adult strain of albino wistar rats were used, which included six normal, diabetic untreated and twenty four diabetic treated rats. Diabetes mellitus was induced by single intraperitoneally injection of 60 mg/kg body weight dose of streptozotocin dissolved in 0.1 ml fresh cold citrate buffer pH 4.5 into 16 h-fasted rats. Diabetic rats were randomly divided as follows: Group I served as normal control, Group II served as diabetic untreated rats, while Group III to Group VI received 200, 400 and 800mg/kg b w of the extract and glibenclamide 10mg/kg b w respectively by orally by gavages for a period of 28 days. The animals were weighed weekly to determine the change in body weight. Fasting blood glucose was measured after every seven days. After the last day of treatment, blood samples were collected from the animals from each group on the 29 th day by cardiac puncture in 16 hours fasted animals for the determination of haematological parameters. The results obtained in this present study showed that the blood glucose level was 46 significantly (p<0.05) decreased in the animals administered with 200mg/kg b w of the extract, with a higher decrease (p < 0.01) observed in the group that received 400 and 800mg/kg b w extract respectively when compared to the diabetic control group. However, there was a significantly increased (p<0.05) body weight of diabetic animals that received all doses of the extract after 14 th , 21 st and 28 th day when compared to diabetic control group. There was a statistically significant increase (p<0.05) in packed cell volume, red cell count, haemoglobin concentration and total protein in the extract treated diabetic animals. The total white blood cell count and lymphocyte revealed a significantly (p<0.05) increased the levels after treatment with the extract after 28 th days. In conclusion, the plant extract showed a significant hypoglycemic as well as erythropoetic effects in the diabetic animals, justifying its use traditionally in the management of diabetes mellitus.
Yemeni Journal for Medical Sciences, 2016
Objective: To evaluate the hypoglycemic activity of Boswellia carterii and Cissus rotundifolia in rats compared to that of glibenclamide and metformin as common oral hypoglycemic drugs. Methods: Thirty-six male Wistar rats, divided into six groups of six rats each, were assigned into diabetic and nondiabetic groups. Diabetes was induced in rats by single intraperitoneal administration of streptozotocin (65 mg/kg b.w.) and nicotinamide (110 mg/kg b.w.). The first two groups were normal and diabetic controls, whereas the other four diabetic groups were treated with water extracts of the medicinal plants; B. carterii (100 mg/kg b.w.) and C. rotundifolia (100 mg/kg b.w.), glibenclamide (5 mg/kg b.w.) and metformin (150 mg/kg b.w.). Body weight and serum glucose were measured on days 1, 7, 14, 21 and 28. Serum cholesterol and triglyceride levels were also measured. Results: Treatment of diabetic rats with the water extracts of B. carterii and C. rotundifolia for four weeks resulted in a significant (p<0.05) increase in their body weights and a significant decrease in the levels of serum glucose, cholesterol and triglycerides. The effects of the two plant extracts were almost similar to those of glibenclamide and metformin. Conclusion: Water extracts of B. carterii or C. rotundifolia have a hypoglycemic effect resembling those of glibenclamide and metformin, and these findings provide a pharmacological evidence for their anti-diabetic claims in folk medicine.