Supplemental Issue of National Seminar on Empowering and Empanelling Ayurveda System of Medicine Organized by SC Mutha Aryangla Vaidyak Mahavidyalaya, Satara Quality control of herbal drugs through UV-Vis spectrophotometric analysis (original) (raw)
Related papers
Simple spectrophotometric methods for standardizing ayurvedic formulation
Indian Journal of Pharmaceutical Sciences, 2012
Vador, et al.: Spectrophotometric Methods for Ayurvedic Formulations Traditional medicines are effective but the standardization of Ayurvedic formulations is essential in order to assess the quality of drugs, based on the concentration of their active principles. Department of AYUSH has given preliminary guidelines for standardizing these conventional formulations, for uniformity of batches in production of Ayurvedic formulation and it is necessary to develop methods for evaluation. The present work is an attempt to standardize asav-arishta, the traditional Ayurvedic formulation using simple, non-expensive spectrophotometric methods. The various parameters performed included total phenolics, total flavonoids, total alkaloids and total saponins, also included pH, sugar %, alcohol content and specific gravity. The results obtained may be considered as tools for assistance to the regulatory authorities, scientific organizations and manufacturers for developing standards.
Pharmaceutical Biology, 2010
Herbal medicines play an important role in modern human life and have significant effects on treating diseases; however, the quality and safety of these herbal products has now become a serious issue due to increasing pollution in air, water, soil, etc. The present study proposes Fourier transform infrared spectroscopy (FTIR) along with the statistical method principal component analysis (PCA) to identify and discriminate herbal medicines for quality control. Herbal plants have been characterized using FTIR spectroscopy. Characteristic peaks (strong and weak) have been marked for each herbal sample in the fingerprint region (400-2000 cm −1 ). The ratio of the areas of any two marked characteristic peaks was found to be nearly consistent for the same plant from different regions, and thus the present idea suggests an additional discrimination method for herbal medicines. PCA clusters herbal medicines into different groups, clearly showing that this method can adequately discriminate different herbal medicines using FTIR data. Toxic metal contents (Cd, Pb, Cr, and As) have been determined and the results compared with the higher permissible daily intake limit of heavy metals proposed by the World Health Organization (WHO).
Standardization, evaluation and quantification of herbal drugs by various analytical methods
International Journal of Ayurvedic Medicine
This review article encompasses the various evaluation parameters of standardization of different medicinal plants and its constituents. In today’s life, more and more people of the world are turning towards the use of natural or herbal products as these have very less or negligible side effects. As the use of herbal medicinal products is increasing day by day, the questions regarding their quality are also arising. This is due to lack of parameters for the standardization of these products. There is a need to set different parameters or methods that can help to standardize the herbal medicinal plant materials. Various methods like physical, chemical, biological is used for standardization and quality evaluation of herbal medicinal plants. These methods can act as a basic tool for the quality evaluation of herbal plant materials. Different parameters of standardization are the fundamental tool for evaluating and assuring the quality of the herbal plant material and its products. Thi...
International Journal of Research in Ayurveda and Pharmacy
The efficacy and quality of commercially manufactured "Maha Varthikava watee" preparations may differ from its authentic preparation prepared as per original recipe. Therefore, a study was carried out to investigate whether the physico-chemical properties, HPLC and UV chromatographic and spectrophotometric measurements can be used for standardization of the above drug. Purified herbals used in the original recipe of "Maha Varthikava watee", were finely powdered and mixed. The mixture was ground together with three juices extracted from leaves of three different species and bee's honey to make pills. Five commercial samples purchased from the market were compared with the authentically prepared samples. Physical properties, chemical properties and chromatographic and spectrophotometric measurements were considered as tools for standardization. For the HPLC and UV spectrophotometry, ethanol extraction was used. Data were analyzed at 0.05 significant levels. No significant differences were observed in any commercial samples with regard to fiber content, acid insoluble ash, dichloromethene extract, ethyl acetate extract and methanol extract while the weight of pills was significantly different. The pH values and loss on drying were significantly different only in two commercial samples. Specific gravity and ash values were found to be different only in one sample. Hexane extract value was significantly different in one sample. In HPLC chromatograms, one main peak was more prominent in three commercial samples. In UV spectrophotometric measurement, two commercial samples had a λ max of 287-290 nm while other three samples had a λ max of 340-345 nm. Hence, these measurements can be used for the standardization of "Maha Varthikava watee".
Chemical marker compounds and their essential role in quality control of herbal medicines
2012
Many countries are registering their standardized herbal drugs with proven clinical efficacy, safety and as dietary supplements. But India is unable to exploit the World market due to unsatisfactory system of quality control which necessitates the establishment of standards for herbal single drugs and compound formulations. In order to ensure international community to use AYUSH drugs with confidence and without compunction, developing acceptable quality control standards of herbal medicines aims to ensure their consistency, safety and efficacy with good manufacturing practices is very much essential. Study of chemical markers is applicable to many research areas, including authentication of genuine species, search for new resources or substitutes of raw materials, optimization of extraction and purification methods, structure elucidation and purity determination. Systematic investigations using chemical markers may lead to discoveries and development of new drugs.
Journal of evidence-based integrative medicine
Rasa Shastra is an exclusive branch of ayurveda that uses processed metals and minerals in various combinations. Though the formulations are time tested, safety and quality concerns are being raised since the past two decades. In view of this, it becomes mandatory to generate quality control profiles of such formulations by following available parameters. Considering this, we attempted to develop standard manufacturing procedures of Maha Yogaraja Guggulu and generate preliminary physicochemical profiles using inductively coupled plasma mass spectrometry, X-ray diffraction, and high-performance thin-layer chromatography. The results from high-performance thin-layer chromatography revealed presence of organic constituents from plant material. X-ray diffraction indicated that the prepared drug contained cinnabar (mercury sulfide; Rasa sindhura), cassiterite (tin oxide; Vanga bhasma), litharge (lead oxide; Naga bhasma), and iron dioxide and magnetite (di-iron oxide; Loha and Mandura bha...
European Journal of Preventive Medicine, 2015
Traditional or alternative medicines such as Ayurvedic drugs are getting importance and reputation day by day in the treatment of various diseases. This is because it has fewer side effects and gives necessary pharmacological action. But prolonged and excess usage may lead to harmful effects such as damage of the heart muscle leading to various types of arrhythmia and coronary artery diseases. "Khadirarista", an Ayurvedic drug, was traditionally used for heart diseases along with jaundice, anemia, abdominal tumor and leprosy. The physicochemical properties such as pH, density, viscosity, conductivity, loss on drying (LOD), chromatographic and spectral study, and presence of metal ions the drug "Khadirarista" were studied. It was found that pH values were 4.53, 4.21 and 4.31 at 1%, 5% and 10% of test solution, respectively. It indicated that "Khadirarishta" was an acidic preparation. Density, average viscosity and residue (LOD) of the drug was 1.0076 g/mL, 25.22 pascal/second and 9.33 g/100 mL, respectively. The spectral study of the test sample was performed and peaks were obtained at the wavelengths of 275, 254, 274.80, 254.60, 275.60, 273.60, 211, 253.60 nm in UV spectrophotometer with 276.60 nm being the λ max. HPLC analysis of the sample was performed and retention time (R t) of the possible active components were found to be 4.12, 4.22 and 4.83 min. R t at 4.12 min was the most sharp peak. Applying TLC, the R f values of the active compounds were found to be 0.6 and 0.7. The level of various metal ions in the sample was measured by Flame Photometry and Na, K, Ca levels were found to be 75 ppm, 5 ppm, 12 ppm, respectively.
Concept of standardization, extraction and pre phytochemical screening strategies for herbal drug
Standardization of drugs means confirmation of its identity and determination of its quality and purity. At present due to advancement in the chemical knowledge of crude drugs various methods like botanical, chemical, spectroscopic and biological methods are used for estimating active constituents present in the crude drugs in addition to its physical constants. Plants have been known to relieve various diseases in Ayurveda. Therefore, the researchers today are emphasizing on evaluation and characterization of various plants and plant constituents against a number of diseases based on their traditional claims of the plants given in Ayurveda. Extraction of the bioactive plant constituents has always been a challenging task for the researchers. In this present review, an attempt has been made to give an overview of certain extractants and extraction processes with their advantages and disadvantages.
Ancient science of life
Herbo-mineral formulations of Ayurveda contain specified metals or minerals as composition, which have their beneficial effects on biological systems. These metals or minerals are transformed into non-toxic forms through meticulous procedures explained in Ayurveda. Though literature is available on quality aspects of such herbo-mineral formulations; contemporary science is raising concerns at regular intervals on such formulations. Thus, it becomes mandate to develop quality profiles of all formulations that contain metals or minerals in their composition. Considering this, it is planned to evaluate analytical profile of Vasantakusumākara Rasa. To prepare Vasantakusumākara Rasa as per Standard operating Procedures (SoP) mentioned in classical text and to characterize it chemically using modern analytical techniques. The drug (Vasantakusumākara Rasa) in three batches was prepared in GMP certified pharmacy. Physico-chemical analysis, Assay of elements and HPTLC were carried out as per...