Determination of Tetracycline Antibiotic Residues in Edible Swine Tissues by Liquid Chromatography with Spectrofluorometric Detection and Confirmation by Mass Spectrometry (original) (raw)
Related papers
Iranian Journal of Pharmaceutical Research (2012), 11 (3): 781-787
Edible cattle tissues from local markets of Ardabil, Iran were examined for residues of tetracycline antibiotics (tetracycline, oxytetracycline and chlortetracycline). In total, 110 samples of triceps, gluteal muscle, diaphragm, kidney and liver were randomly obtained from the local markets of the city of Ardabil. Solid-phase extraction (SPE) and highperformance liquid chromatography (HPLC) methods were used to extract and analyze tetracycline antibiotics (TC) residues, respectively. The mean amount of total TC residues in all samples tested was 226.3 ± 112.5 ng/g; the mean amount of the total TC residues in triceps, gluteal muscle, diaphragm, kidney and liver samples were 176.3 ± 46.8, 405.3 ± 219.6, 96.8 ± 26.9, 672.4 ± 192.0 and 651.3 ± 210.1 ng/g, respectively. Additionally, 25.8% of muscle samples, 31.8% of liver samples and 22.7% of kidney samples contained amounts of TC residues beyond the maximum residue limit (MRLs). To reduce the TC residues found in edible cattle tissues, regulatory authorities should ensure that cattle undergo the proper withdrawal period from TCs before slaughtering. Keywords: Tetracycline; Meat; Liver; Kidney; HPLC.
Overview on liquid chromatographic analysis of tetracycline residues in food matrices
Food Chemistry, 2011
Tetracycline antibiotics (TCs) are often used as feed additives for food-producing animals because of the wide Gram (+) and Gram (À) antibacterial spectrum, low toxicity profile and more importantly low cost. However, antibiotic residues in food, produced from those animals, might be high due to misuse of TCs. Depending on the level of antibiotic residues, a negative impact on human health might be expected. The risk for public health resulting from the use of these veterinary medications has always been a though question to answer for health authorities around the world. Therefore, it is very important to determine residues of TCs in food accurately with appropriate analytical methods that are optimised, validated and implementing new advanced techniques. In this paper, liquid chromatographic methods that are available for the determination of TC levels in foods and food products are reviewed.
Chromatographic methods for tetracycline analysis in foods
Journal of Chromatography A, 1992
have served for decades as an important class of antibiotics in food animal health and production. As such, they have also been a source of concern for residue monitoring authorities around the world. In response to this concern a number of microbial inhibition, immunoassay and bacterial receptor methods have evolved for the detection of this class of compounds in various foods of animal origin. However, these methods often lack specificity and are subject to false positive and false negative results. For these reasons a number of chromatographic methods for the separation and determination of the tetracyclines isolated from foods have been developed that are capable of identifying and quantifying individual tetracycline drugs. We present here an overview of tetracycline analytical methods, including microbial inhibition, immunoassay and receptor technologies for detection, techniques for isolation from food matrices, and thin-layer chromatographic, high-performance liquid chromatographic, gas chromatographic and mass spectrometric procedures for determination of this class of compounds.
Monitoring and risk assessment of tetracycline residues in foods of animal origin
Food Science and Biotechnology, 2019
A total of 450 samples consisting of red meat, poultry meat, aquatic product and raw milk were collected during winter 2016 and summer 2017. 22.2% (100/450) of collected meat and raw milk samples were found to be contaminated with antibiotic residues in the initial screening using Premi Ò test. According to the enzyme linked immunosorbent assay (ELISA) results, the mean tetracyclines (TCs) concentration of meat samples determined as follows: chicken (155.41 lg/kg) [ turkey (138.68 lg/kg) [ quail (130.7 lg/kg) [ cow (108.92 lg/ kg) [ calf (105.18 lg/kg) [ goat (99.4 lg/kg) [ sheep (95.22 lg/kg) [ rainbow trout (35.62 lg/kg) [ shrimp (31.80 lg/kg). The content of TCs in cow, goat and sheep milk samples were found to be ranged 45.6-163.5 lg/L, 72.4-101.1 lg/L and 65.5-98.9 lg/L, respectively. 5.7% (26/450) of samples had TCs confirmed the ELISA results using high performance liquid chromatography coupled with ultraviolet detection, although the concentration of TCs residues in samples was higher than that of ELISA.
Current Research on Biosciences and Biotechnology, 2022
The objective of the study was to quantify tetracycline (TCs) i.e., oxytetracycline (OTC), tetracycline (TC) and chlortetracycline (CTC) residues in thirty poultry meat samples (n = 30) collected from the local market and super shop around the Dhaka University campus during May 2019 to January 2020. Three samples were collected from each of ten locations. All samples were extracted by Quick, Easy, Cheap, Effective, Rugged and Safe (QuEChERS) method. Samples were analyzed by reversed-phase High-Performance Liquid Chromatograph equipped with Photo Diode Array Detector (LC-PDA) and a reported method was validated with good linear correlation coefficients of standards and matrix-matched calibration curves with r2=1.00, 0.99, 0.99 and r2= 0.99, 0.99,0.99 in the linearity range of 0-10 µg/kg for OTC, TC and CTC, respectively. The limit of detection (LOD) and limit of quantification (LOQ) for OTC, TC, and CTC were 1.05, 1.17, and 1.09 μg/kg and 3.15, 3.51 and 3.27 μg/kg, respectively. Accu...
Journal of Analytical Methods in Chemistry, 2016
The Commission Regulation 574/2011/EC set up maximum levels of coccidiostats and histomonostats in nonmedicated feed as a consequence of carry-over during manufacturing. Carry-over takes place from medicated to nonmedicated feed during feed production. Similar contamination could also occur for other pharmaceuticals such as tetracyclines, a group of antibiotics commonly employed in food production animal. The objective of this work is to present a simple and fast method for the simultaneous detection of four tetracyclines (chlortetracycline, doxycycline, oxytetracycline, and tetracycline) in nontarget feed at aμg/kg level. Validation of the method was performed according to the guideline included in the Commission Decision 2002/657/EC for official method. The validated method was successfully applied to 50 feed samples collected from different milk farms and 25 samples obtained from feed manufacturers. While oxytetracycline was the tetracycline most frequently detected, chlortetracy...
Al-Mustansiriyah Journal of Science
The aim of this research is separation and determination of trace Tetracycline residues in Poultry chest, thigh and liver using High Performance Liquid Chromatography - Diode Array Detector (HPLC-DAD), with a mobile phase mixture consisting of acetonitrile: methanol: oxalic acid (0.01M) (25:15:60) and chromatographic column C8. The study was done on 32 live poultry individuals. All individuals were injected in the chest by 1m of Tetracycline standard solutions, then slaughter for analysis throughout four successive days. The injection with 10×103 ppb of Tetracycline showed that the traces of Tetracycline residues (according to the Codex Alimentarius Commission) exceeded the value of the maximum residue limit (MRL = 200 ppb) in the thigh and chest meat at the 1st day and the 1st & 2nd days of slay respectively, and exceeded the value of (MRL = 600 ppb) in the liver at the 3rd and 4th days of slay. limit of detection was LOD = 0.451 ppb, limit of quantification LOQ = 1.502 ppb, and re...
Rapid Detection of Tetracycline Residues in Chicken
International Journal of Applied Sciences and Biotechnology, 2020
Antibiotic residues in meat are a serious public health concern as a result of its harmful effects on consumer health. This study aimed at estimating the residues levels of commonly used antibiotics (Tetracycline) in chicken samples using two analytical methods; Rapid Screening Kit and HPLC. Twenty chicken samples were collected from various meat shops of Kathmandu valley. Qualitative and semi-quantitative analyses with Quicking Tetracycline rapid Test kit, which detect tetracycline above 100 ppb in tissue revealed that two samples were positive for antibiotic residues in the chicken for tetracycline. The concentration of residues of tetracycline, which were positive in rapid test kit was quantified by high-performance liquid chromatography equipped with a UV detector (HPLC-UV). One muscle sample and one liver sample of chicken have found the concentration of tetracycline as 229 and 339 μg/kg, respectively while chlortetracycline was not detected. This result confirmed widespread mi...
Validation Study for the Determination of Tetracycline Residues in Animal Tissues
Journal of AOAC INTERNATIONAL, 1999
An interlaboratory study of the liquid chromatographic (LC) determination of tetracyclines—oxytetracycline (OTC), tetracycline (TTC), and chlortetracycline (CTC)—in animal tissues was conducted. Isolation was performed with oxalic buffer followed by dechelation and deproteination with oxalic acid–acetonitrile. The extract was cleaned with a styrene–divinylbenzene cartridge. LC analysis was performed with a PLRP-S column and 0.01 M oxalic acid–acetonitrile (75 + 25, v/v) as mobile phase. Participants analyzed 2 control and 10 fortified porcine muscle and kidney samples. Additionally, porcine muscle samples containing incurred residues of tetracyclines were analyzed. Mean recoveries of fortified residues from porcine tissue ranged from 76.00 to 86.89%. Repeatabilities varied from 2.05% for OTC to 3.61 % for TTC for muscle samples and from 6.75% for CTC to 8.74% for OTC for kidney samples. Reproducibilities ranged from 2.05 to 4.30% for muscle samples and from 15.77 to 18.81 % for kidn...