Mobile Silencing Research Papers - Academia.edu (original) (raw)
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Recent papers in Mobile Silencing
Upward long-distance mobile silencing has been shown to be phloem-mediated in several different Solanaceous species. We show that the Arabidopsis seedling grafting system and a counterpart inducible system generate upwardly spreading long... more
Upward long-distance mobile silencing has been shown to be phloem-mediated in several different Solanaceous species. We show that the Arabidopsis seedling grafting system and a counterpart inducible system generate upwardly spreading long distance silencing that travels not in the phloem but by template-dependent reiterated short-distance cell-to-cell spread through the cells of the central stele. Examining the movement of the silencing front revealed a largely unrecognized zone of tissue, below the apical meristem, that is resistant to the silencing signal and may provide a gating or protective barrier against sRNA signals. Using a range of auxin and actin transport inhibitors revealed that, in this zone, alteration of vesicular transport together with cytoskeleton dynamics prevented or retarded the spread of the silencing signal. This suggests that sRNAs are transported from cell to cell via plasmodesmata rather than diffusing from their source in the phloem.