Sequence, Genomic Distribution and DNA Modification of a Mu1 Element from Non-Mutator Maize Stocks (original) (raw)
- Journal List
- Genetics
- v.119(4); 1988 Aug
- PMC1203478
Genetics. 1988 Aug; 119(4): 951–958.
Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403
Abstract
The increased mutation rate of Mutator stocks of maize has been shown to be the result of transposition of Mu elements. One element, Mu1, is present in 10-60 copies in Mutator stocks and approximately 0-3 copies in non-Mutator stocks. The sequence, structure and genomic distribution of an intact Mu1 element cloned from the non-Mutator inbred line B37 has been determined. The sequence of this element, termed Mu1.4-B37, is identical to Mu1 and it is flanked by 9-bp direct repeats indicative of a target site duplication. Mu1.4-B37 is not in the same genomic location in all stocks, which further suggests that it transposed into its genomic location in B37. We previously reported that in genomic DNA this element is modified such that certain methylation-sensitive restriction enzymes will not cut sites within the element. This is similar to that observed for Mu elements in Mutator stocks that have lost activity. We report herein that the Mu1.4-B37 element loses its modification and becomes accessible to digestion when placed in an active Mutator stock by genetic crosses. This suggests that factors conditioning unmodified elements are dominant in the initial cross between Mutator and non-Mutator stocks. In F(2) individuals that have subsequently lost Mutator activity the Mu1.4-B37 element again becomes modified as do most of the Mu elements in the stock. Thus, the modification state of the Mu1.4-B37 element and the other Mu1-like elements correlates with Mutator activity. We hypothesize that factor(s) within an active Mutator stock may inhibit the modification of Mu elements, and that this activity is missing in non-Mutator stocks and may become limiting in certain Mutator stocks resulting in DNA modification.
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Selected References
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- Alleman M, Freeling M. The Mu transposable elements of maize: evidence for transposition and copy number regulation during development. Genetics. 1986 Jan;112(1):107–119. [PMC free article] [PubMed] [Google Scholar]
- Barker RF, Thompson DV, Talbot DR, Swanson J, Bennetzen JL. Nucleotide sequence of the maize transposable element Mul. Nucleic Acids Res. 1984 Aug 10;12(15):5955–5967. [PMC free article] [PubMed] [Google Scholar]
- Bennetzen JL, Swanson J, Taylor WC, Freeling M. DNA insertion in the first intron of maize Adh1 affects message levels: cloning of progenitor and mutant Adh1 alleles. Proc Natl Acad Sci U S A. 1984 Jul;81(13):4125–4128. [PMC free article] [PubMed] [Google Scholar]
- Chandler VL, Walbot V. DNA modification of a maize transposable element correlates with loss of activity. Proc Natl Acad Sci U S A. 1986 Mar;83(6):1767–1771. [PMC free article] [PubMed] [Google Scholar]
- Chandler V, Rivin C, Walbot V. Stable non-mutator stocks of maize have sequences homologous to the Mu1 transposable element. Genetics. 1986 Nov;114(3):1007–1021. [PMC free article] [PubMed] [Google Scholar]
- Chen J, Greenblatt IM, Dellaporta SL. Transposition of Ac from the P locus of maize into unreplicated chromosomal sites. Genetics. 1987 Sep;117(1):109–116. [PMC free article] [PubMed] [Google Scholar]
- Chen CH, Oishi KK, Kloeckener-Gruissem B, Freeling M. Organ-specific expression of maize Adh1 is altered after a Mu transposon insertion. Genetics. 1987 Jul;116(3):469–477. [PMC free article] [PubMed] [Google Scholar]
- Chomet PS, Wessler S, Dellaporta SL. Inactivation of the maize transposable element Activator (Ac) is associated with its DNA modification. EMBO J. 1987 Feb;6(2):295–302. [PMC free article] [PubMed] [Google Scholar]
- Cone KC, Burr FA, Burr B. Molecular analysis of the maize anthocyanin regulatory locus C1. Proc Natl Acad Sci U S A. 1986 Dec;83(24):9631–9635. [PMC free article] [PubMed] [Google Scholar]
- Devereux J, Haeberli P, Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387–395. [PMC free article] [PubMed] [Google Scholar]
- Döring HP, Starlinger P. Molecular genetics of transposable elements in plants. Annu Rev Genet. 1986;20:175–200. [PubMed] [Google Scholar]
- Emmons SW, Yesner L, Ruan KS, Katzenberg D. Evidence for a transposon in Caenorhabditis elegans. Cell. 1983 Jan;32(1):55–65. [PubMed] [Google Scholar]
- Engels WR. The P family of transposable elements in Drosophila. Annu Rev Genet. 1983;17:315–344. [PubMed] [Google Scholar]
- McCLINTOCK B. Chromosome organization and genic expression. Cold Spring Harb Symp Quant Biol. 1951;16:13–47. [PubMed] [Google Scholar]
- McClintock B. The significance of responses of the genome to challenge. Science. 1984 Nov 16;226(4676):792–801. [PubMed] [Google Scholar]
- McLaughlin M, Walbot V. Cloning of a mutable bz2 allele of maize by transposon tagging and differential hybridization. Genetics. 1987 Dec;117(4):771–776. [PMC free article] [PubMed] [Google Scholar]
- Messing J. New M13 vectors for cloning. Methods Enzymol. 1983;101:20–78. [PubMed] [Google Scholar]
- Mizusawa S, Nishimura S, Seela F. Improvement of the dideoxy chain termination method of DNA sequencing by use of deoxy-7-deazaguanosine triphosphate in place of dGTP. Nucleic Acids Res. 1986 Feb 11;14(3):1319–1324. [PMC free article] [PubMed] [Google Scholar]
- O'Reilly C, Shepherd NS, Pereira A, Schwarz-Sommer Z, Bertram I, Robertson DS, Peterson PA, Saedler H. Molecular cloning of the a1 locus of Zea mays using the transposable elements En and Mu1. EMBO J. 1985 Apr;4(4):877–882. [PMC free article] [PubMed] [Google Scholar]
- Peschke VM, Phillips RL, Gengenbach BG. Discovery of transposable element activity among progeny of tissue culture--derived maize plants. Science. 1987 Nov 6;238(4828):804–807. [PubMed] [Google Scholar]
- Roberts D, Hoopes BC, McClure WR, Kleckner N. IS10 transposition is regulated by DNA adenine methylation. Cell. 1985 Nov;43(1):117–130. [PubMed] [Google Scholar]
- Robertson DS. Genetic studies on the loss of mu mutator activity in maize. Genetics. 1986 Jul;113(3):765–773. [PMC free article] [PubMed] [Google Scholar]
- Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. [PMC free article] [PubMed] [Google Scholar]
- Schmidt RJ, Burr FA, Burr B. Transposon tagging and molecular analysis of the maize regulatory locus opaque-2. Science. 1987 Nov 13;238(4829):960–963. [PubMed] [Google Scholar]
- Taylor LP, Walbot V. Isolation and characterization of a 1.7-kb transposable element from a mutator line of maize. Genetics. 1987 Oct;117(2):297–307. [PMC free article] [PubMed] [Google Scholar]
- Vieira J, Messing J. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene. 1982 Oct;19(3):259–268. [PubMed] [Google Scholar]
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