Bafilomycins: a class of inhibitors of membrane ATPases from microorganisms, animal cells, and plant cells. (original) (raw)

• Journal List
• Proc Natl Acad Sci U S A
• v.85(21); 1988 Nov
• PMC282335

Proc Natl Acad Sci U S A. 1988 Nov; 85(21): 7972–7976.

Department of Biology, University of California, Santa Cruz 95064.

Abstract

Various membrane ATPases have been tested for their sensitivity to bafilomycin A1, a macrolide antibiotic. F1F0 ATPases from bacteria and mitochondria are not affected by this antibiotic. In contrast, E1E2 ATPases--e.g., the K+-dependent (Kdp) ATPase from Escherichia coli, the Na+,K+-ATPase from ox brain, and the Ca2+-ATPase from sarcoplasmic reticulum--are moderately sensitive to this inhibitor. Finally, membrane ATPases from Neurospora vacuoles, chromaffin granules, and plant vacuoles are extremely sensitive. From this we conclude that bafilomycin A1 is a valuable tool for distinguishing among the three different types of ATPases and represents the first relatively specific potent inhibitor of vacuolar ATPases.

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