| dbo:abstract |
A chevron plot is a way of representing protein folding kinetic data in the presence of varying concentrations of denaturant that disrupts the protein's native tertiary structure. The plot is known as "chevron" plot because of the canonical v, or chevron shape observed when the logarithm of the observed is plotted as a function of the denaturant concentration. In a two-state system, folding and unfolding rates dominate the observed relaxation rates below and above the denaturation midpoint (Cm). This gives rise to the terminology of folding and unfolding arms for the limbs of the chevron. A priori information on the Cm of a protein can be obtained from equilibrium experiments. In fitting to a two-state model, the logarithm of the folding and unfolding rates is assumed to depend linearly on the denaturant concentration, thus resulting in the slopes mf and mu, called the folding and unfolding m-values, respectively (also called the kinetic m-values). The sum of the two rates is the observed relaxation rate. An agreement between equilibrium m-value and the absolute sum of the kinetic m-values is typically seen as a signature for two-state behavior. Most of the reported denaturation experiments have been carried out at 298 K with either urea or guanidinium chloride (GuHCl) as denaturants. (en) |
| dbo:thumbnail |
wiki-commons:Special:FilePath/Chevron_plot.jpg?width=300 |
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7618 (xsd:nonNegativeInteger) |
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| dbo:wikiPageWikiLink |
dbr:Urea dbr:Viscosity dbr:Dead_time dbr:Debye–Hückel_equation dbr:Downhill_folding dbr:Chemical_kinetics dbr:Equilibrium_unfolding dbr:Logarithm dbr:Slope dbr:Denaturation_(biochemistry) dbr:Denaturation_midpoint dbr:Transition_state dbc:Protein_structure dbr:Fluorescence dbr:Protein_folding dbr:Guanidinium_chloride dbr:File:Chevron_plot.JPG dbr:Pressure_jump dbc:Protein_methods dbr:Chevron_(insignia) dbr:Tertiary_structure dbr:Circular_dichroism dbr:Spectroscopic dbr:Phi_value_analysis dbr:Temperature_jump dbr:Stopped_flow dbr:Hammond_effect dbr:298_K dbr:Relaxation_rate |
| dbp:wikiPageUsesTemplate |
dbt:Short_description |
| dct:subject |
dbc:Protein_structure dbc:Protein_methods |
| gold:hypernym |
dbr:Way |
| rdf:type |
yago:Ability105616246 yago:Abstraction100002137 yago:Cognition100023271 yago:Know-how105616786 yago:Method105660268 yago:PsychologicalFeature100023100 yago:WikicatProteinMethods |
| rdfs:comment |
A chevron plot is a way of representing protein folding kinetic data in the presence of varying concentrations of denaturant that disrupts the protein's native tertiary structure. The plot is known as "chevron" plot because of the canonical v, or chevron shape observed when the logarithm of the observed is plotted as a function of the denaturant concentration. (en) |
| rdfs:label |
Chevron plot (en) |
| owl:sameAs |
freebase:Chevron plot yago-res:Chevron plot wikidata:Chevron plot https://global.dbpedia.org/id/4i6YP |
| prov:wasDerivedFrom |
wikipedia-en:Chevron_plot?oldid=1040480893&ns=0 |
| foaf:depiction |
wiki-commons:Special:FilePath/Chevron_plot.jpg |
| foaf:isPrimaryTopicOf |
wikipedia-en:Chevron_plot |
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dbr:Chevron |
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dbr:Chevron_plots |
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dbr:Chevron dbr:Protein_folding dbr:Chevron_plots dbr:Phi_value_analysis |
| is foaf:primaryTopic of |
wikipedia-en:Chevron_plot |
