| dbo:abstract |
Le MNase-Seq est une technique utilisée en biologie moléculaire pour identifier les régions de la chromatine occupées par des nucléosomes à l'échelle du génome. (fr) MNase-seq, short for micrococcal nuclease digestion with deep sequencing, is a molecular biological technique that was first pioneered in 2006 to measure nucleosome occupancy in the C. elegans genome, and was subsequently applied to the human genome in 2008. Though, the term ‘MNase-seq’ had not been coined until a year later, in 2009. Briefly, this technique relies on the use of the non-specific endo-exonuclease micrococcal nuclease, an enzyme derived from the bacteria Staphylococcus aureus, to bind and cleave protein-unbound regions of DNA on chromatin. DNA bound to histones or other chromatin-bound proteins (e.g. transcription factors) may remain undigested. The uncut DNA is then purified from the proteins and sequenced through one or more of the various Next-Generation sequencing methods. MNase-seq is one of four classes of methods used for assessing the status of the epigenome through analysis of chromatin accessibility. The other three techniques are DNase-seq, FAIRE-seq, and ATAC-seq. While MNase-seq is primarily used to sequence regions of DNA bound by histones or other chromatin-bound proteins, the other three are commonly used for: mapping Deoxyribonuclease I hypersensitive sites (DHSs), sequencing the DNA unbound by chromatin proteins, or sequencing regions of loosely packaged chromatin through transposition of markers, respectively. (en) MNase-seq是micrococcal nuclease digestion with deep sequencing（微球菌核酸酶消化结合深度测序）的缩写，是2008年以来用于检测人类基因组上核小体占用情况的分子生物学技术。在2009年才定名“MNase-seq”。简而言之，该技术依赖于来自金黄色葡萄球菌的非特异性，用其结合和切割染色质上未结合蛋白质的DNA区域。与此同时，此种酶不消化与组蛋白或其他染色质结合蛋白（例如转录因子）结合的DNA。然后从蛋白质中纯化未切割的DNA，并通过一种或多种不同的下一代测序方法进行测序。 MNase-seq是用于通过分析染色质可及性来评估表观基因组状态的四类方法之一。其他三种技术是、和ATAC-seq。MNase-seq主要用于对由组蛋白或其他染色质结合蛋白结合的DNA区域进行测序，其他三种测序的对象通常分别是：（DHS）、测未结合的DNA的染色质蛋白质和松散包裹的染色质区域（通过标记物的测定）。 (zh) |
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Le MNase-Seq est une technique utilisée en biologie moléculaire pour identifier les régions de la chromatine occupées par des nucléosomes à l'échelle du génome. (fr) MNase-seq是micrococcal nuclease digestion with deep sequencing（微球菌核酸酶消化结合深度测序）的缩写，是2008年以来用于检测人类基因组上核小体占用情况的分子生物学技术。在2009年才定名“MNase-seq”。简而言之，该技术依赖于来自金黄色葡萄球菌的非特异性，用其结合和切割染色质上未结合蛋白质的DNA区域。与此同时，此种酶不消化与组蛋白或其他染色质结合蛋白（例如转录因子）结合的DNA。然后从蛋白质中纯化未切割的DNA，并通过一种或多种不同的下一代测序方法进行测序。 MNase-seq是用于通过分析染色质可及性来评估表观基因组状态的四类方法之一。其他三种技术是、和ATAC-seq。MNase-seq主要用于对由组蛋白或其他染色质结合蛋白结合的DNA区域进行测序，其他三种测序的对象通常分别是：（DHS）、测未结合的DNA的染色质蛋白质和松散包裹的染色质区域（通过标记物的测定）。 (zh) MNase-seq, short for micrococcal nuclease digestion with deep sequencing, is a molecular biological technique that was first pioneered in 2006 to measure nucleosome occupancy in the C. elegans genome, and was subsequently applied to the human genome in 2008. Though, the term ‘MNase-seq’ had not been coined until a year later, in 2009. Briefly, this technique relies on the use of the non-specific endo-exonuclease micrococcal nuclease, an enzyme derived from the bacteria Staphylococcus aureus, to bind and cleave protein-unbound regions of DNA on chromatin. DNA bound to histones or other chromatin-bound proteins (e.g. transcription factors) may remain undigested. The uncut DNA is then purified from the proteins and sequenced through one or more of the various Next-Generation sequencing method (en) |
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