Demonstration of cytomegalovirus nucleic acids in the coronary arteries of transplanted hearts. (original) (raw)
- Journal List
- Am J Pathol
- v.140(3); 1992 Mar
- PMC1886167
Am J Pathol. 1992 Mar; 140(3): 739–747.
Abstract
Cytomegalovirus (CMV) infection has been associated with the development of accelerated arteriosclerosis in heart transplant recipients. This association prompted the authors to examine, using in situ hybridization techniques, the coronary arteries of 19 patients who had received heart transplants for the presence of CMV nucleic acids. Two probes were employed on formalin-fixed paraffin-embedded tissues. The first was an S35-labeled nick translated probe derived from CMV DNA. CMV nucleic acids were identified in the arterial intima of 5 (45%) of 11 transplanted hearts examined with this probe. Hybridization to cells morphologically consistent with endothelial cells, lymphocytes, and smooth muscle cells was identified. Hybridization was not detected in any of the hearts when a control probe, a vector without CMV DNA, was employed. The second probe that was used was a tritium-labeled strand-specific ribo-probe derived from the immediate early (IE1) gene of CMV. Using this second probe, hybridization was detected in the coronary arteries of three of five hearts that were positive with the DNA probe, in one of the hearts that was negative with the DNA probe, and in three (38%) of eight additional transplanted hearts. The pattern of hybridization was the same as that seen with the DNA probe, and no hybridization was detected when a control sense probe for the IE1 gene of CMV was employed. These results suggest that CMV nucleic acids are present in the coronary arteries of heart transplant recipients.
Full text
Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.6M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.
Images in this article
Click on the image to see a larger version.
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Dummer JS, White LT, Ho M, Griffith BP, Hardesty RL, Bahnson HT. Morbidity of cytomegalovirus infection in recipients of heart or heart-lung transplants who received cyclosporine. J Infect Dis. 1985 Dec;152(6):1182–1191. [PubMed] [Google Scholar]
- Wreghitt TG, Hakim M, Gray JJ, Kucia S, Cory-Pearce R, Wallwork J, English TA. A detailed study of cytomegalovirus infections in the first 160 heart and heart/lung transplant recipients at Papworth Hospital, Cambridge, England. Transplant Proc. 1987 Feb;19(1 Pt 3):2495–2496. [PubMed] [Google Scholar]
- Millett R, Tomita T, Marshall HE, Cohen L, Hannah H., 3rd Cytomegalovirus endomyocarditis in a transplanted heart. A case report with in situ hybridization. Arch Pathol Lab Med. 1991 May;115(5):511–515. [PubMed] [Google Scholar]
- Fryd DS, Peterson PK, Ferguson RM, Simmons RL, Balfour HH, Jr, Najarian JS. Cytomegalovirus as a risk factor in renal transplantation. Transplantation. 1980 Dec;30(6):436–439. [PubMed] [Google Scholar]
- Peterson PK, Balfour HH, Jr, Marker SC, Fryd DS, Howard RJ, Simmons RL. Cytomegalovirus disease in renal allograft recipients: a prospective study of the clinical features, risk factors and impact on renal transplantation. Medicine (Baltimore) 1980 Jul;59(4):283–300. [PubMed] [Google Scholar]
- Grattan MT, Moreno-Cabral CE, Starnes VA, Oyer PE, Stinson EB, Shumway NE. Cytomegalovirus infection is associated with cardiac allograft rejection and atherosclerosis. JAMA. 1989 Jun 23;261(24):3561–3566. [PubMed] [Google Scholar]
- Rees AP, Meadors M, Ventura HO, Pankey GA. Diagnosis of disseminated cytomegalovirus infection and pneumonitis in a heart transplant recipient by skin biopsy: case report. J Heart Lung Transplant. 1991 Mar-Apr;10(2):329–332. [PubMed] [Google Scholar]
- Preiksaitis JK, Rosno S, Grumet C, Merigan TC. Infections due to herpesviruses in cardiac transplant recipients: role of the donor heart and immunosuppressive therapy. J Infect Dis. 1983 Jun;147(6):974–981. [PubMed] [Google Scholar]
- Light JA, Burke DS. Association of cytomegalovirus (CMV) infections with increased recipient mortality following transplantation. Transplant Proc. 1979 Mar;11(1):79–82. [PubMed] [Google Scholar]
- Petrie BL, Melnick JL, Adam E, Burek J, McCollum CH, DeBakey ME. Nucleic acid sequences of cytomegalovirus in cells cultured from human arterial tissue. J Infect Dis. 1987 Jan;155(1):158–159. [PubMed] [Google Scholar]
- Yamashiroya HM, Ghosh L, Yang R, Robertson AL., Jr Herpesviridae in the coronary arteries and aorta of young trauma victims. Am J Pathol. 1988 Jan;130(1):71–79. [PMC free article] [PubMed] [Google Scholar]
- Hruban RH, Beschorner WE, Baumgartner WA, Augustine SM, Ren H, Reitz BA, Hutchins GM. Accelerated arteriosclerosis in heart transplant recipients is associated with a T-lymphocyte-mediated endothelialitis. Am J Pathol. 1990 Oct;137(4):871–882. [PMC free article] [PubMed] [Google Scholar]
- Ambinder RF, Newman C, Hayward GS, Biggar R, Melbye M, Kestens L, Van Marck E, Piot P, Gigase P, Wright PB, et al. Lack of association of cytomegalovirus with endemic African Kaposi's sarcoma. J Infect Dis. 1987 Jul;156(1):193–197. [PubMed] [Google Scholar]
- Pizzorno MC, O'Hare P, Sha L, LaFemina RL, Hayward GS. trans-activation and autoregulation of gene expression by the immediate-early region 2 gene products of human cytomegalovirus. J Virol. 1988 Apr;62(4):1167–1179. [PMC free article] [PubMed] [Google Scholar]
- Wu TC, Mounts P. Sensitive detection of nucleic acids and protein of human papillomavirus type 6 in respiratory and genital tract papillomata. J Virol Methods. 1989 Jul;25(1):31–47. [PubMed] [Google Scholar]
- Wu TC, Mann RB, Charache P, Hayward SD, Staal S, Lambe BC, Ambinder RF. Detection of EBV gene expression in Reed-Sternberg cells of Hodgkin's disease. Int J Cancer. 1990 Nov 15;46(5):801–804. [PubMed] [Google Scholar]
- Wu TC, Mann RB, Epstein JI, MacMahon E, Lee WA, Charache P, Hayward SD, Kurman RJ, Hayward GS, Ambinder RF. Abundant expression of EBER1 small nuclear RNA in nasopharyngeal carcinoma. A morphologically distinctive target for detection of Epstein-Barr virus in formalin-fixed paraffin-embedded carcinoma specimens. Am J Pathol. 1991 Jun;138(6):1461–1469. [PMC free article] [PubMed] [Google Scholar]
- Hruban RH, Wu TC, Beschorner WE, Cameron DE, Ambinder RF, Baumgartner WA, Reitz BA, Hutchins GM. Cytomegalovirus nucleic acids in allografted hearts. Hum Pathol. 1990 Sep;21(9):981–982. [PubMed] [Google Scholar]
- Srinivasappa J, Saegusa J, Prabhakar BS, Gentry MK, Buchmeier MJ, Wiktor TJ, Koprowski H, Oldstone MB, Notkins AL. Molecular mimicry: frequency of reactivity of monoclonal antiviral antibodies with normal tissues. J Virol. 1986 Jan;57(1):397–401. [PMC free article] [PubMed] [Google Scholar]
- McDonald K, Rector TS, Braulin EA, Kubo SH, Olivari MT. Association of coronary artery disease in cardiac transplant recipients with cytomegalovirus infection. Am J Cardiol. 1989 Aug 1;64(5):359–362. [PubMed] [Google Scholar]
- Rice GP, Schrier RD, Oldstone MB. Cytomegalovirus infects human lymphocytes and monocytes: virus expression is restricted to immediate-early gene products. Proc Natl Acad Sci U S A. 1984 Oct;81(19):6134–6138. [PMC free article] [PubMed] [Google Scholar]
- Gyorkey F, Melnick JL, Guinn GA, Gyorkey P, DeBakey ME. Herpesviridae in the endothelial and smooth muscle cells of the proximal aorta in arteriosclerotic patients. Exp Mol Pathol. 1984 Jun;40(3):328–339. [PubMed] [Google Scholar]
- Benditt EP, Barrett T, McDougall JK. Viruses in the etiology of atherosclerosis. Proc Natl Acad Sci U S A. 1983 Oct;80(20):6386–6389. [PMC free article] [PubMed] [Google Scholar]
- Hendrix MG, Dormans PH, Kitslaar P, Bosman F, Bruggeman CA. The presence of cytomegalovirus nucleic acids in arterial walls of atherosclerotic and nonatherosclerotic patients. Am J Pathol. 1989 May;134(5):1151–1157. [PMC free article] [PubMed] [Google Scholar]
- Hendrix MG, Salimans MM, van Boven CP, Bruggeman CA. High prevalence of latently present cytomegalovirus in arterial walls of patients suffering from grade III atherosclerosis. Am J Pathol. 1990 Jan;136(1):23–28. [PMC free article] [PubMed] [Google Scholar]
- Hendrix MG, Daemen M, Bruggeman CA. Cytomegalovirus nucleic acid distribution within the human vascular tree. Am J Pathol. 1991 Mar;138(3):563–567. [PMC free article] [PubMed] [Google Scholar]
- Benditt EP, Barrett T, McDougall JK. Viruses in the etiology of atherosclerosis. Proc Natl Acad Sci U S A. 1983 Oct;80(20):6386–6389. [PMC free article] [PubMed] [Google Scholar]
- Toorkey CB, Carrigan DR. Immunohistochemical detection of an immediate early antigen of human cytomegalovirus in normal tissues. J Infect Dis. 1989 Nov;160(5):741–751. [PubMed] [Google Scholar]
- Melnick JL, Petrie BL, Dreesman GR, Burek J, McCollum CH, DeBakey ME. Cytomegalovirus antigen within human arterial smooth muscle cells. Lancet. 1983 Sep 17;2(8351):644–647. [PubMed] [Google Scholar]
- Myerson D, Hackman RC, Nelson JA, Ward DC, McDougall JK. Widespread presence of histologically occult cytomegalovirus. Hum Pathol. 1984 May;15(5):430–439. [PubMed] [Google Scholar]
- Tumilowicz JJ, Gawlik ME, Powell BB, Trentin JJ. Replication of cytomegalovirus in human arterial smooth muscle cells. J Virol. 1985 Dec;56(3):839–845. [PMC free article] [PubMed] [Google Scholar]
- Schrier RD, Nelson JA, Oldstone MB. Detection of human cytomegalovirus in peripheral blood lymphocytes in a natural infection. Science. 1985 Nov 29;230(4729):1048–1051. [PubMed] [Google Scholar]
- Gnann JW, Jr, Ahlmén J, Svalander C, Olding L, Oldstone MB, Nelson JA. Inflammatory cells in transplanted kidneys are infected by human cytomegalovirus. Am J Pathol. 1988 Aug;132(2):239–248. [PMC free article] [PubMed] [Google Scholar]
- Chou SW. Acquisition of donor strains of cytomegalovirus by renal-transplant recipients. N Engl J Med. 1986 May 29;314(22):1418–1423. [PubMed] [Google Scholar]
- Fabricant CG, Fabricant J, Litrenta MM, Minick CR. Virus-induced atherosclerosis. J Exp Med. 1978 Jul 1;148(1):335–340. [PMC free article] [PubMed] [Google Scholar]
- Minick CR, Fabricant CG, Fabricant J, Litrenta MM. Atheroarteriosclerosis induced by infection with a herpesvirus. Am J Pathol. 1979 Sep;96(3):673–706. [PMC free article] [PubMed] [Google Scholar]
- Fabricant CG, Hajjar DP, Minick CR, Fabricant J. Herpesvirus infection enhances cholesterol and cholesteryl ester accumulation in cultured arterial smooth muscle cells. Am J Pathol. 1981 Nov;105(2):176–184. [PMC free article] [PubMed] [Google Scholar]
- Ross R. Mechanisms of atherosclerosis--a review. Adv Nephrol Necker Hosp. 1990;19:79–86. [PubMed] [Google Scholar]
- Visser MR, Tracy PB, Vercellotti GM, Goodman JL, White JG, Jacob HS. Enhanced thrombin generation and platelet binding on herpes simplex virus-infected endothelium. Proc Natl Acad Sci U S A. 1988 Nov;85(21):8227–8230. [PMC free article] [PubMed] [Google Scholar]
- Etingin OR, Silverstein RL, Friedman HM, Hajjar DP. Viral activation of the coagulation cascade: molecular interactions at the surface of infected endothelial cells. Cell. 1990 May 18;61(4):657–662. [PubMed] [Google Scholar]
- van Es A, Baldwin WM, Oljans PJ, Tanke HJ, Ploem JS, van Es LA. Expression of HLA-DR on T lymphocytes following renal transplantation, and association with graft-rejection episodes and cytomegalovirus infection. Transplantation. 1984 Jan;37(1):65–69. [PubMed] [Google Scholar]
- von Willebrand E, Pettersson E, Ahonen J, Häyry P. CMV infection, class II antigen expression, and human kidney allograft rejection. Transplantation. 1986 Oct;42(4):364–367. [PubMed] [Google Scholar]
- Fujinami RS, Nelson JA, Walker L, Oldstone MB. Sequence homology and immunologic cross-reactivity of human cytomegalovirus with HLA-DR beta chain: a means for graft rejection and immunosuppression. J Virol. 1988 Jan;62(1):100–105. [PMC free article] [PubMed] [Google Scholar]
- Hajjar DP, Nicholson AC, Hajjar KA, Sando GN, Summers BD. Decreased messenger RNA translation in herpesvirus-infected arterial cells: effects on cholesteryl ester hydrolase. Proc Natl Acad Sci U S A. 1989 May;86(9):3366–3370. [PMC free article] [PubMed] [Google Scholar]
- Hajjar DP, Fabricant CG, Minick CR, Fabricant J. Virus-induced atherosclerosis. Herpesvirus infection alters aortic cholesterol metabolism and accumulation. Am J Pathol. 1986 Jan;122(1):62–70. [PMC free article] [PubMed] [Google Scholar]
- Albrecht T, Rapp F. Malignant transformation of hamster embryo fibroblasts following exposure to ultraviolet-irradiated human cytomegalovirus. Virology. 1973 Sep;55(1):53–61. [PubMed] [Google Scholar]
- Benditt EP. Implications of the monoclonal character of human atherosclerotic plaques. Ann N Y Acad Sci. 1976;275:96–100. [PubMed] [Google Scholar]
- Plotkin SA, Starr SE, Friedman HM, Brayman K, Harris S, Jackson S, Tustin NB, Grossman R, Dafoe D, Barker C. Effect of Towne live virus vaccine on cytomegalovirus disease after renal transplant. A controlled trial. Ann Intern Med. 1991 Apr 1;114(7):525–531. [PubMed] [Google Scholar]
- Brayman KL, Dafoe DC, Smythe WR, Barker CF, Perloff LJ, Naji A, Fox IJ, Grossman RA, Jorkasky DK, Starr SE, et al. Prophylaxis of serious cytomegalovirus infection in renal transplant candidates using live human cytomegalovirus vaccine. Interim results of a randomized controlled trial. Arch Surg. 1988 Dec;123(12):1502–1508. [PubMed] [Google Scholar]
Articles from The American Journal of Pathology are provided here courtesy of American Society for Investigative Pathology