Emerging colistin resistance in Salmonella enterica serovar Newport isolates from human infections - PubMed (original) (raw)

Emerging colistin resistance in Salmonella enterica serovar Newport isolates from human infections

Mohammed Elbediwi et al. Emerg Microbes Infect. 2020.

Abstract

Worldwide emergence of Salmonella enterica serovar Newport (S. Newport) infection in humans, in parallel with a significant increasing prevalence of antimicrobial resistance (AR), is a serious public health concern. However, the prevalence of S. Newport resistance in China remains largely unknown. A retrospective study of 287 S. Newport clinical isolates collected during 1997-2018 was undertaken for characterization of AR profiles using the micro-dilution assay. We found a recent emergence of colistin resistance in four Chinese clinical isolates, including _mcr_-1-positive isolates. Importantly, phylogenomic and microbiological investigations indicate multiple independent clonal transmission of colistin-resistant S. Newport isolates of different seafood origins. Our study highlights potential reservoirs for transmission of colistin resistance and suggests that the global food supply chain may facilitate this dissemination.

Keywords: Salmonella Newport; antimicrobial resistance; colistin resistance; mcr-1; seafood.

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Conflict of interest statement

No potential conflict of interest was reported by the author(s).

Figures

Figure 1.

Figure 1.

Comparative sequence analysis of the _mcr_-carrying plasmids and phylogenomic features for all Chinese Salmonella Newport genomes available in the NCBI database in addition to four new colistin-resistant strains in this study. (a) Sequence comparison of two reconstructed _mcr_-1-positive plasmids from whole-genome sequence. The out layer circle refer to the CP01924.2 plasmid. (b) Genetic backbone flanking with _mcr_-1 sequence of SAL_ 276 and SAL_ 311 (CP019214.2 in E. coli isolated from sewage in China and CP033347.2 in S. Typhimurium isolated from pork in China). IS, insertion sequence; HP, hypothetical protein; PM, putative membrane; REC, recombinase. (c) Phylogenomic analysis of all available S. Newport Chinese isolates in this study, including 24 additional Chinese S. Newport isolates from different hosts retrieved from the NCBI database. The four isolates examined in this study in addition to their closely related seafood-origin isolates were clustered together in the three subclades (A, B and C). Tree scale represents the genetic distance between the isolates used to construct the tree. *These isolates were collected by US FDA, mentioning China as their place of origin.

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This work was supported by the National Program on Key Research Project of China [grant number 2017YFC1600103, 2018YFD0500501, SQ2019YFE010999] as well as Horizon 2020 Framework Programme under Grant Agreement No. 861917 – SAFFI and Zhejiang Provincial Natural Science Foundation [grant number LR19C180001].

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