Efficient genome editing in zebrafish using a CRISPR-Cas system (original) (raw)
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Acknowledgements
This work was supported by a US National Institutes of Health (NIH) Director's Pioneer Award DP1 GM105378 (J.K.J.), NIH R01 GM088040 (J.K.J. & R.T.P.), NIH P50 HG005550 (J.K.J.), the Jim and Ann Orr Research Scholar Award (J.K.J.), NIH K01 AG031300 (J.-R.J.Y.) and a Massachusetts General Hospital Claflin award (J.-R.J.Y.). We thank G. Church, J. Aach and P. Mali for sharing unpublished results and helpful discussions.
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- Woong Y Hwang and Yanfang Fu: These authors contributed equally to this work.
Authors and Affiliations
- Cardiovascular Research Center, Massachusetts General Hospital, Charlestown, Massachusetts, USA
Woong Y Hwang, Randall T Peterson & J-R Joanna Yeh - Molecular Pathology Unit, Center for Cancer Research, and Center for Computational and Integrative Biology, Massachusetts General Hospital, Charlestown, Massachusetts, USA
Yanfang Fu, Deepak Reyon, Morgan L Maeder, Shengdar Q Tsai, Jeffry D Sander & J Keith Joung - Department of Pathology, Harvard Medical School, Boston, Massachusetts, USA
Yanfang Fu, Deepak Reyon, Shengdar Q Tsai, Jeffry D Sander & J Keith Joung - Program in Biological and Biomedical Sciences, Harvard Medical School, Boston, Massachusetts, USA
Morgan L Maeder & J Keith Joung - Department of Medicine, Harvard Medical School, Boston, Massachusetts, USA
Randall T Peterson & J-R Joanna Yeh - Broad Institute, Cambridge, Massachusetts, USA
Randall T Peterson
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- Woong Y Hwang
You can also search for this author inPubMed Google Scholar - Yanfang Fu
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Contributions
W.Y.H., Y.F., M.L.M., D.R., S.Q.T., J.D.S., R.T.P., J.-R.J.Y. and J.K.J. designed experiments. W.Y.H., Y.F., M.L.M., D.R., S.Q.T. and J.D.S. performed experiments. W.Y.H., Y.F., M.L.M., D.R., S.Q.T., J.D.S., R.T.P., J.-R.J.Y. and J.K.J. wrote the paper.
Corresponding authors
Correspondence toJ-R Joanna Yeh or J Keith Joung.
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Competing interests
J.K.J. has a financial interest in Transposagen Biopharmaceuticals.
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Hwang, W., Fu, Y., Reyon, D. et al. Efficient genome editing in zebrafish using a CRISPR-Cas system.Nat Biotechnol 31, 227–229 (2013). https://doi.org/10.1038/nbt.2501
- Received: 18 December 2012
- Accepted: 10 January 2013
- Published: 29 January 2013
- Issue Date: March 2013
- DOI: https://doi.org/10.1038/nbt.2501