Efficient genome editing in zebrafish using a CRISPR-Cas system (original) (raw)

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Acknowledgements

This work was supported by a US National Institutes of Health (NIH) Director's Pioneer Award DP1 GM105378 (J.K.J.), NIH R01 GM088040 (J.K.J. & R.T.P.), NIH P50 HG005550 (J.K.J.), the Jim and Ann Orr Research Scholar Award (J.K.J.), NIH K01 AG031300 (J.-R.J.Y.) and a Massachusetts General Hospital Claflin award (J.-R.J.Y.). We thank G. Church, J. Aach and P. Mali for sharing unpublished results and helpful discussions.

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Author notes

  1. Woong Y Hwang and Yanfang Fu: These authors contributed equally to this work.

Authors and Affiliations

  1. Cardiovascular Research Center, Massachusetts General Hospital, Charlestown, Massachusetts, USA
    Woong Y Hwang, Randall T Peterson & J-R Joanna Yeh
  2. Molecular Pathology Unit, Center for Cancer Research, and Center for Computational and Integrative Biology, Massachusetts General Hospital, Charlestown, Massachusetts, USA
    Yanfang Fu, Deepak Reyon, Morgan L Maeder, Shengdar Q Tsai, Jeffry D Sander & J Keith Joung
  3. Department of Pathology, Harvard Medical School, Boston, Massachusetts, USA
    Yanfang Fu, Deepak Reyon, Shengdar Q Tsai, Jeffry D Sander & J Keith Joung
  4. Program in Biological and Biomedical Sciences, Harvard Medical School, Boston, Massachusetts, USA
    Morgan L Maeder & J Keith Joung
  5. Department of Medicine, Harvard Medical School, Boston, Massachusetts, USA
    Randall T Peterson & J-R Joanna Yeh
  6. Broad Institute, Cambridge, Massachusetts, USA
    Randall T Peterson

Authors

  1. Woong Y Hwang
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  2. Yanfang Fu
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  3. Deepak Reyon
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  4. Morgan L Maeder
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  5. Shengdar Q Tsai
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  6. Jeffry D Sander
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  7. Randall T Peterson
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  8. J-R Joanna Yeh
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  9. J Keith Joung
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Contributions

W.Y.H., Y.F., M.L.M., D.R., S.Q.T., J.D.S., R.T.P., J.-R.J.Y. and J.K.J. designed experiments. W.Y.H., Y.F., M.L.M., D.R., S.Q.T. and J.D.S. performed experiments. W.Y.H., Y.F., M.L.M., D.R., S.Q.T., J.D.S., R.T.P., J.-R.J.Y. and J.K.J. wrote the paper.

Corresponding authors

Correspondence toJ-R Joanna Yeh or J Keith Joung.

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Competing interests

J.K.J. has a financial interest in Transposagen Biopharmaceuticals.

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Hwang, W., Fu, Y., Reyon, D. et al. Efficient genome editing in zebrafish using a CRISPR-Cas system.Nat Biotechnol 31, 227–229 (2013). https://doi.org/10.1038/nbt.2501

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