Structural analysis of multicellular organisms with cryo-electron tomography (original) (raw)

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Acknowledgements

We thank Y. Gruenbaum (The Hebrew University of Jerusalem) for providing us with the strains of C. elegans used in this study. This work was supported by a European Research Council (ERC) Starting Grant (243047 INCEL) and the Swiss National Science Foundation (SNSF 31003A_141083/1).

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Authors and Affiliations

  1. Department of Biochemistry, University of Zurich, Zurich, Switzerland
    Jan Harapin, Mandy Börmel, K Tanuj Sapra & Ohad Medalia
  2. Institute of Molecular Life Sciences, University of Zurich, Zurich, Switzerland
    Mandy Börmel & Damian Brunner
  3. Center for Microscopy and Image Analysis, University of Zurich, Zurich, Switzerland
    Andres Kaech
  4. Department of Life Sciences in the Negev, Ben-Gurion University, Beer-Sheva, Israel
    Ohad Medalia
  5. National Institute for Biotechnology in the Negev, Ben-Gurion University, Beer-Sheva, Israel
    Ohad Medalia

Authors

  1. Jan Harapin
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  2. Mandy Börmel
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  3. K Tanuj Sapra
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  4. Damian Brunner
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  5. Andres Kaech
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  6. Ohad Medalia
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Contributions

J.H., A.K., K.T.S., D.B. and O.M. designed the experiments and wrote the manuscript. J.H. and M.B. carried out the experiments.

Corresponding authors

Correspondence toAndres Kaech or Ohad Medalia.

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The authors declare no competing financial interests.

Integrated supplementary information

Supplementary Figure 1 Cryo-holder design and overview of the cryo-FIB-SEM procedure carried out on embryos and worms

(a) The prototype cryo-holder. The attachment slit (black arrow) and shutter (black asterisk) were redesigned to accommodate standard EM grids at a 6° angle with respect to the FIB column in the Auriga crossbeam system. (b) The two-piece attachment slit is regulated by a screw (black arrow head) on the upper component (red arrow head), while the base is freely movable (green arrow head). (c) The aluminum shutter envelops the cryo-holder while attached to the tip of the rod of the VCT100 shuttle via a Teflon ring. (d) The cryo-holder inside the Auriga. (e) SEM image of a well-vitrified embryo. The lamella is flat and no cracks are present. (f) SEM image of a cracked embryo. Cracks in the sample (white arrow heads) can be observed immediately upon the removal of the top of the sample with the cryo-FIB. (g) Cryo-TEM image of an embryo lamella showing the full dimensions of a lamella used for tomographic imaging. White asterisk indicates areas used for focusing and tracking and white arrows show the direction of the Ga+ beam milling. (h) SEM image of a vitrified worm (white arrows) after the sublimation of 2-methylpentane. (i) FIB image of the worm lamella (area in between white arrow heads) after milling. (j) SEM image of the worm lamella from top showing the full dimensions of the milled area (white double arrow) and the non-milled sides (black double arrows). Scale bars represent 2 µm (g), 5 µm (e,i), 10 µm (f,j), and 100 µm (h).

Supplementary Figure 2 Cryo-electron microscopy of gold nanoparticles and CET of vitrified C. elegans worms

(a) Cryo-TEM image of gold nanoparticles synthesized in toluene and spread on the surface of a plunge frozen EM grid. The grid is covered by ice contamination that varies both in thickness and size (white asterisk) and shows the markers clustering within this contamination (black arrows). (b) Cryo-TEM image of gold nanoparticles synthesized in 2-methylpentane and spread on the surface of a plunge-frozen EM grid. Individual markers are evenly distributed along the grid surface and contamination is barely detectable (compare the areas indicated by the white asterisks). (c,e) 3.4 nm thick tomographic slices acquired with a –16 µm defocus on a worm cryo-lamella. Green arrow heads point towards filamentous structures adjacent to the INM, the black arrow points to a plasma membrane protrusion and the white asterisks indicate the position of NPCs. (N – nucleus, C – Cytoplasm). (d) A surface rendered view corresponding to the black-framed area in panel a. Plasma membrane – dark blue, nuclear membrane – pink, ribosomes – gold, NPCs – light blue, and filamentous structures adjacent to the INM – green. Final resolution of both tomograms was estimated from the first zero of the CTF and calculated to be 5.4 nm. Scale bars represent 250 nm (a,b) and 200 nm (c,e).

Supplementary Figure 3 Correlative light and electron microscopy of C. elegans embryos expressing lamin-GFP

(a) SEM overview of the grid before milling. The embryo that will be milled is indicated by the white arrow. (b) Bright-field-fluorescence channel overlay showing the same embryo post-milling, fixed in 4% PFA (white arrow). (c) SEM image of the embryo post-milling. White asterisk indicated the area where platinum was deposited inside the cryo-FIB-SEM device to facilitate milling. (d) Bright-field-fluorescence channel overlay showing bright lamin-GFP signal inside the thicker, non-milled sides of the embryo, and its distribution throughout the thin lamella. The white asterisk indicated the area where platinum was deposited (same as in panel c). The bright-field-fluorescence images (b,d) are rotated approximately 40° clock-wise compared to the SEM images (a,c). Scale bars indicate 200 µm (a), 50 µm (b), 5 µm (c), and 10 µm (d).

Supplementary Figure 4 Cryo-FIB milling of Drosophila melanogaster embryos

(a) SEM overview image of the EM grid bearing 3 D. melanogaster embryos (red arrows). (b) FIB side view of the edge of the embryo after several rounds of milling with high currents. (c) FIB side view of the thin lamella after successive rounds of course and fine milling. The area underneath the lamella is also cleared in order to facilitate tomographic imaging. (d) SEM top view of the lamella after milling. Black double arrows indicate the non-milled slopes surrounding the lamella and the white double arrows indicate the lamella itself. Scale bars indicate 500 µm (a) and 50 µm (b–d).

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Harapin, J., Börmel, M., Sapra, K. et al. Structural analysis of multicellular organisms with cryo-electron tomography.Nat Methods 12, 634–636 (2015). https://doi.org/10.1038/nmeth.3401

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