Crystal structure of a chaperone complex that contributes to the assembly of yeast 20S proteasomes (original) (raw)

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Acknowledgements

We thank all of the members of BL44XU, especially E. Yamashita and M. Yoshimura, for their help in data collection at SPring-8 and T. Hikage for his help in X-ray diffraction data collection for PAC3. This work was supported by grants from Japan Science and Technology Agency (to S.M.), the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan (to H.Y., T.M., S.M., E.K., K.K. and K. Tanaka) and the Target Protein Project of MEXT (to T.M., K.K. and K. Tanaka and the Takeda Science Foundation (to K. Tanaka)). E.S. is a recipient of a Japan Society for the Promotion of Science Research Fellowship for Young Scientists.

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Author notes

  1. Hideki Yashiroda and Tsunehiro Mizushima: These authors contributed equally to this work.

Authors and Affiliations

  1. Laboratory of Frontier Science, Core Technology and Research Center, Tokyo Metropolitan Institute of Medical Science, Bunkyo-ku, Tokyo, 113-8613, Japan
    Hideki Yashiroda, Tomie Kameyama, Eri Sakata, Yuko Hirano, Shigeo Murata & Keiji Tanaka
  2. Department of Biotechnology, Graduate School of Engineering, Nagoya University, Chikusa-ku, Nagoya, 464-8603, Japan
    Tsunehiro Mizushima, Kenji Takagi, Atsuo Suzuki & Takashi Yamane
  3. Department of Structural Biology and Biomolecular Engineering, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Mizuho-ku, Nagoya, 467-8603, Japan
    Kenta Okamoto, Eiji Kurimoto, Eri Sakata & Koichi Kato
  4. Link Genomics, Inc., Chuo-ku, Tokyo, 103-0024, Japan
    Hidemi Hayashi & Shin-ichiro Niwa
  5. Proteome Analysis Center, Toho University, Funabashi, Chiba, 274-8510, Japan
    Hidemi Hayashi & Toshihiko Kishimoto
  6. Department of Biomolecular Science, Faculty of Science, Toho University, Funabashi, Chiba, 274-8510, Japan
    Toshihiko Kishimoto
  7. Department of Pathology, Hokkaido University Graduate School of Medicine, Sapporo, Hokkaido, 060-8638, Japan
    Masanori Kasahara
  8. Laboratory of Protein Metabolism, Graduate School of Pharmaceutical Sciences, the University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan
    Shigeo Murata
  9. Institute for Molecular Science, National Institutes of Natural Sciences, 5-1 Higashi-yama, Myodaiji, Okazaki, 444-8787, Japan
    Koichi Kato

Authors

  1. Hideki Yashiroda
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  2. Tsunehiro Mizushima
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  3. Kenta Okamoto
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  4. Tomie Kameyama
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  5. Hidemi Hayashi
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  6. Toshihiko Kishimoto
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  7. Shin-ichiro Niwa
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  8. Masanori Kasahara
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  9. Eiji Kurimoto
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  10. Eri Sakata
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  11. Kenji Takagi
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  12. Atsuo Suzuki
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  13. Yuko Hirano
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  14. Shigeo Murata
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  15. Koichi Kato
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  16. Takashi Yamane
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  17. Keiji Tanaka
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Contributions

H.Y. and T. Kameyama performed all of the yeast experiments. T.M., H.Y., K. Takagi and T.Y. determined the structures of the Dmp1–Dmp2 and Dmp1–Dmp2 Δloop-α5 complexes. K.O., E.K., E.S., A.S., Y.H., S.M., T.Y. and K.K. determined the structure of PAC3. H.H., T. Kishimoto and S.N. conducted the mass spectrometric analysis. M.K. performed phylogenetic analyses. H.Y., T.M., K.K., M.K. and K. Tanaka wrote the paper. All of the authors discussed the results and commented on the manuscript.

Corresponding author

Correspondence toKeiji Tanaka.

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Yashiroda, H., Mizushima, T., Okamoto, K. et al. Crystal structure of a chaperone complex that contributes to the assembly of yeast 20S proteasomes.Nat Struct Mol Biol 15, 228–236 (2008). https://doi.org/10.1038/nsmb.1386

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