Dominant-activating germline mutations in the gene encoding the PI(3)K catalytic subunit p110δ result in T cell senescence and human immunodeficiency (original) (raw)

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References

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Acknowledgements

We thank the referring physicians, as well as the patients and families. Supported by the Division of Intramural Research, National Institute of Allergy and Infectious Diseases, Clinical Center of the US National Institutes of Health (C.L.L., H.S.K., J.E.N., M.B., J.S., W.O., V.K.R., A.A., A.A.H., K.N.O., T.A.F., S.P., S.M.H., J.I.C., M.J.L., G.U.), the National Human Genome Research Institute of the US National Institutes of Health (F.Z., J.L.C., P.L.S.), the Frederick National Laboratory for Cancer Research of the US National Institutes of Health (HHSN261200800001E), the National Health and Medical Research Council of Australia (E.K.D., U.P., S.G.T.), Cancer Council NSW (S.G.T.), the Cancer Institute NSW (U.P.), the Research Foundation-Flanders, Belgium (L.M.) and the National Institute of General Medical Sciences (C.L.L. and R.Z.). The content of this publication does not necessarily reflect the views or policies of the US Department of Health and Human Services, nor does mention of trade names, commercial products or organizations imply endorsement by the US Government.

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Author notes

1. Carrie L Lucas, Hye Sun Kuehn and Fang Zhao: These authors contributed equally to this work.

Authors and Affiliations

1. Molecular Development of the Immune System Section, Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA
   Carrie L Lucas, Matthew Biancalana, V Koneti Rao & Michael J Lenardo
2. Department of Laboratory Medicine, Clinical Center, National Institutes of Health, Bethesda, Maryland, USA
   Hye Sun Kuehn, Julie E Niemela, Jennifer Stoddard & Thomas A Fleisher
3. Cell Signaling Section, Genetic Disease Research Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland, USA
   Fang Zhao, Jennifer L Cannons & Pamela L Schwartzberg
4. Pritzker School of Medicine, The University of Chicago, Chicago, Illinois, USA
   Fang Zhao
5. Immunology and Immunodeficiency Group, Immunology Program, Garvan Institute of Medical Research, Sydney, Australia
   Elissa K Deenick, Umaimainthan Palendira, Danielle T Avery, Leen Moens & Stuart G Tangye
6. St. Vincent's Clinical School Faculty of Medicine, University of New South Wales, Sydney, Australia
   Elissa K Deenick, Umaimainthan Palendira & Stuart G Tangye
7. Division of Monoclonal Antibodies, Laboratory of Cell Biology, Office of Biotechnology Products, Center for Drug Evaluation and Research, United States Food and Drug Administration, Bethesda, Maryland, USA
   Weiming Ouyang & David M Frucht
8. Division of Allergy and Immunology, Department of Pediatrics, University of Alabama at Birmingham, Birmingham, Alabama, USA
   T Prescott Atkinson
9. Laboratory of Clinical Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA
   Anahita Agharahimi, Ashleigh A Hussey, Kenneth N Olivier, Steven M Holland & Gulbu Uzel
10. Laboratory of Clinical Infectious Diseases, Clinical Research Directorate–Clinical Monitoring Research Program, Science Applications International Corporation–Frederick, Frederick National Laboratory for Clinical Research, Frederick, Maryland, USA
    Anahita Agharahimi
11. Radiology and Imaging and Sciences, Clinical Center, National Institutes of Health, Bethesda, Maryland, USA
    Les R Folio
12. Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA
    Stefania Pittaluga
13. Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA
    Jeffrey I Cohen
14. Instituto de Medicina Integral Prof. Fernando Figueira, Recife-Pernambuco, Brazil
    Joao B Oliveira

Authors

1. Carrie L Lucas
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2. Hye Sun Kuehn
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3. Fang Zhao
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4. Julie E Niemela
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5. Elissa K Deenick
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6. Umaimainthan Palendira
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7. Danielle T Avery
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8. Leen Moens
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9. Jennifer L Cannons
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10. Matthew Biancalana
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11. Jennifer Stoddard
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12. Weiming Ouyang
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13. David M Frucht
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14. V Koneti Rao
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15. T Prescott Atkinson
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16. Anahita Agharahimi
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17. Ashleigh A Hussey
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18. Les R Folio
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19. Kenneth N Olivier
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20. Thomas A Fleisher
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21. Stefania Pittaluga
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22. Steven M Holland
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23. Jeffrey I Cohen
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24. Joao B Oliveira
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25. Stuart G Tangye
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26. Pamela L Schwartzberg
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27. Michael J Lenardo
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28. Gulbu Uzel
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Contributions

C.L.L did experiments, analyzed data and developed and wrote the manuscript; H.S.K., F.Z., E.K.D., U.P., D.T.A., L.M. and J.L.C. did experiments and analyzed data; J.E.N. analyzed genomic DNA sequencing and bioinformatics, discovered candidate genes and analyzed protein structure; M.B. analyzed p110 structure; J.S. did experiments and analyzed genomic DNA sequencing and data; W.O. did experiments; D.M.F. supervised research and data analysis; V.K.R. evaluated patients and collected data; T.P.A. and J.I.C. provided patient access, clinical data, samples and advice; A.A. evaluated patients and collected and analyzed data; A.A.H. coordinated patient access, data collection and analysis; L.R.F. evaluated and prepared data from clinical imaging studies; K.N.O. evaluated patients and collected and analyzed data; T.A.F. supervised research and data analysis and provided advice; S.P. did histological and immunohistochemical analyses of patient samples; S.M.H. supervised research and data analysis and provided advice; J.B.O planned and supervised whole-exome sequencing experiments; S.G.T. planned and supervised experiments, analyzed data, provided advice and prepared the manuscript; P.L.S. planned and supervised experiments, analyzed data and provided advice; M.J.L. supervised research and data analysis, provided advice and prepared the manuscript; G.U. coordinated research efforts, supervised research work and data analysis, and prepared the manuscript; and all authors discussed and revised the manuscript.

Corresponding author

Correspondence toGulbu Uzel.

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Competing interests

The authors declare no competing financial interests.

Additional information

Note added in proof: Another study has now independently described a cohort of patients with the E1021K substitution of p110δ[48](/articles/ni.2771#ref-CR48 "Angulo, I. et al. Phosphoinositide 3-kinase δ gene mutation predisposes to respiratory infection and airway damage. Science doi:10.1126/science.1243292

             (17 October 2013).").

Integrated supplementary information

Supplementary Figure 1 Lymphadenopathy, mitogen response, effector cells, and serum Ig assessment.

(a) Computerized Tomography (CT) images demonstrating lymphadenopathy (arrows) in patient F.II.1 and absence of spleen (removed surgically). (b) Three dimensional surface rendered image with cut plane at level of porta hepatis showing lymph node masses surrounding the portal vein and adjacent vasculature (darker red mass within white oval). (c) 3H-Thymidine incorporation in counts per minute (CPM) to assess proliferation of PBMCs after three days in response to the indicated stimulus. Each symbol represents an individual healthy control subject (Ctrl, n = 10) or patient (Pt, n = 10). Small horizontal lines indicate mean (± s.d.). *P < 0.0001, **P = 0.0002, ***P < 0.0001, ****P < 0.0001 (Mann-Whitney test). (d) Cumulative data for multiple healthy controls (Ctrl, n = 8) and patients (Pt, n = 9) showing % CCR7− negative cells among CD8+ T cells in the peripheral blood. Small horizontal lines indicate mean (± s.d.). *P < 0.0001 (unpaired _t_-test). (e) IgM (left) and IgA (right) serum concentrations as a function of the patient's age. Dotted lines indicate the upper and lower boundaries of the normal range from healthy subjects. Data are representative of ten independent experiments with one patient each (c), four independent experiments (d) or two to ten separate measurements over time for each of the nine indicated patients.

Supplementary Figure 2 Patient B cell defects.

(a) The proportion of total B cells (i.e., CD20+ cells) within the lymphocyte population of healthy controls (Ctrl, n = 6) compared to patients (Pt, n = 7). Small horizontal lines indicate mean (± s.d.). (b, c) Expression of (b) CD5 on transitional and naïve B cells and of (c) surface IgG and IgA on memory B cells from healthy controls (red histogram) and patients (blue histogram) was determined by flow cytometry. (d) CFSE-labeled naïve B cells were cultured with CD40L, CD40L+CpG, CD40L+anti-Ig or CD40L together with IL-4, IL-10, or IL-21 for 5 days before being assessed for dilution of the dye in healthy control (Ctrl, red) and patient (Pt, blue) samples. (e) Expression of AICDA mRNA was determined by quantitative PCR after stimulation of naïve B cells with the indicated stimulus. (f,g) Secretion of IgM, IgG, and IgA by stimulated naïve (f) and memory (g) B cells was determined by ELISA. Values for healthy controls (Ctrl) and patients (Pt) are given as mean ± sem of replicate cultures. *P = 0.03, **P = 0.005 (unpaired _t_-test). Data are representative of four independent experiments (b,c), two independent experiments (e), three independent experiments (f) or two independent experiments (g) or are from five independent experiments (a) or one experiment (d).

Supplementary Figure 4 Hyperphosphorylation of Akt and intact association of mutant p110δ with p85α.

(a) Quantification of band intensities for the immunoblot shown in Fig. 2d in which serum-starved, activated T cells were assessed for p110δ, Akt, p-Akt (S473), and β-tubulin in patients D.II.1, D.II.2, and E.1 compared to three healthy controls (Ctrl) with 10 min anti-CD3 stimulation (+) or not (−). Total Akt values were normalized to β-tubulin abundance, then the ratio of p-Akt (S473) to normalized Akt was graphed. Small horizontal lines indicate mean (± s.d.). *P = 0.0002, **P = 0.01, ***P = 0.008 (unpaired _t_-test). (b) Summary of p-Akt (S473) by flow cytometry comparing mean fluorescence intensity (MFI). Basal p-Akt (S473) in paired (i.e., cultured for the same duration) T cell blasts from healthy controls (Ctrl, n = 5) and patients (Pt, n = 5) (left) or induced p-Akt (S473) after 10 min stimulation with anti-CD3 in T cell blasts from Ctrl (n = 3) and Pt (n = 3) (right, with mean (± s.d.)) are shown. *P = 0.02 (paired _t_-test), **P = 0.03 (unpaired _t_-test). (c) Immunoblots for the indicated proteins using PBMC lysates from patients with mutant p110δ or healthy controls (Ctrl) after stimulation with beads coated with anti-CD3 and anti-CD28 for 10 min (+) or not (?). (d,e) Representative histograms (left) and cumulative MFI data (right) for flow cytometry staining for p-Akt (T308) in patient (Pt, n = 3) versus healthy control (Ctrl, n = 3) T cell blasts, gating on CD4+ or CD8+ cells. Small horizontal lines indicate mean (± s.d.). *P = 0.03 (unpaired _t_-test). (f) Quantification of band intensities for the immunoblot shown in Fig. 2e together with data from two additional independent experiments. Numbers indicate the relative ratio (WT set to 1.0) of p-Akt (S473) to total Akt normalized for Flag expression. Small horizontal lines indicate mean (± s.d.). *P = 0.05, **P = 0.009 (unpaired _t_-test). (g) Immunoblot for p-Akt (S473) and β-tubulin on lysates from the human H9 T cell line overexpressing WT or the indicated mutant p110δ. (h) Immunoprecipitates (IP) of empty vector (EV), wild-type (WT), or mutant p110δ fused to a Flag epitope in HEK293T cells overexpressing p85α were blotted with antibodies against p85α, the Flag epitope, β-actin or immunoglobulin heavy chain (IgH). Data are from one experiment with three patients (a) or three independent experiments (f) or are representative of three independent experiments (b,d,e), four independent experiments with one patient each (c) or two independent experiments (g,h).

Supplementary Figure 5 Patient CD8+ T cells are hyperactivated with characteristics of enhanced effector function.

(a) Cumulative data for MHC class I-peptide tetramer (Tet) stains for EBV lytic (Ctrl n = 4 and Pt n = 4) and EBV latent antigens (Ctrl n = 3 and Pt n = 3). (b) Flow cytometry of gated EBV lytic and latent antigen-specific CD8+ T cells, stained for CCR7 and CD45RA. The percent of total events is shown in quadrants. (c) CD38 expression on the populations identified in (a) for patient G.1 (blue) compared to those for a healthy control (Ctrl, red). (d) Cumulative data for intracellular IFN-γ production in CD8+ T cell blasts from healthy controls (Ctrl, n = 3) versus patients (Pt, n = 4). Small horizontal lines indicate mean (± s.d.). P = 0.09 (unpaired _t_-test). (e) Cumulative data for granzyme B expression in CD4+ or CD8+ T cell blasts stimulated with low-dose, immobilized anti-CD3. Small horizontal lines indicate mean (± s.d.). *P = 0.003, **P = 0.01 (unpaired _t_-test). (f) Total LAMP-1 levels in activated CD8+ T cells from patients A.1 and G.1 compared to healthy control (Ctrl). (g) Cytolysis of P815 targets by anti-CD3-mediated redirected lysis for the indicated patients (Pt, n = 4) and healthy controls (Ctrl, n = 4). (h) Composite data for % CD57+ CD8+ T cells in patient (Pt, n = 4) versus healthy control (Ctrl, n = 4) PBMCs. Small horizontal lines indicate mean (± s.d.). *P = 0.002 (unpaired _t_-test). Data are from three (latent) or four (lytic) independent experiments (a) or three independent experiments (h) or are representative of four independent experiments (b,c,g) or two independent experiments (d,e,f).

Supplementary Figure 6 Expression of PD-1 on CD3+ cells in peripheral blood.

PBMCs were gated on CD3+ cells and assessed for CD45RA versus PD-1 expression in indicated patients (Pt, n = 7) and healthy controls (Ctrl, n = 2 adult and n = 2 pediatric). Data are representative of four independent experiments.

Supplementary Figure 7 Normal expression of signaling molecules in patient T cell blasts.

(a) Immunoblots for PTEN, PKC , p110δ, p-Erk, total Erk, and β-tubulin in T cell lysates from patients E.1 and F.II.1 or healthy control (Ctrl) cells stimulated with anti-CD3 for the times indicated in minutes (min). (b) Immunoblot for the PH domain-containing ARF6, p27kip1, and β-tubulin in indicated patient (n = 5 samples) and healthy control (Ctrl, n = 5 samples) T cell blasts after 10-min stimulation with anti-CD3 (+) or not (−). Data are from one experiment with two (a) or five (b) different patient samples.

Supplementary Figure 8 Increased, glucose- and amino acid-dependent basal S6 phosphorylation and glucose uptake in patient T cell blasts.

(a) Cumulative flow cytometric analysis of mean fluorescence intensity (MFI) for stimulated and unstimulated, permeabilized patient (Pt) or healthy control (Ctrl) T cell blasts after staining for p-S6 (S235, S236). Paired healthy control and patient blasts (i.e., cultured for the same duration) are connected by a line. *P = 0.04 (paired _t_-test). (b) Data from the same experiment as that shown in Fig. 4c but where cells were first rested in PBS for 2 hr before flow cytometric analysis of p- S6 (S235, S236) in unstimulated T cell blasts from patients G.1, D.II.1, and D.II.2 compared to healthy controls (Ctrl). (c) Glucose uptake by flow cytometric analysis of 2-NBDG fluorescence (linear MFI) after a 20-minute incubation period in glucose-starved T cell blasts from healthy controls (Ctrl, n = 3) and patients (Pt, n = 3). Paired healthy control and patient blasts (i.e., cultured for the same duration) are connected by a line. *P = 0.03 (paired _t_-test). Data are from three independent experiments (a) or one experiment with three different patients (b) or are representative of five independent experiments (c).

Supplementary Figure 9 CT images comparing hepatosplenomegaly and lymphadenopathy in patient A.1 before and after treatment with rapamycin.

Coronal reformations at the level of the tracheal bifurcation reveal more severe hepatosplenomegaly, hilar and mediastinal adenopathy, and splaying of tracheal bifurcation before (a) compared to after (b) rapamycin treatment.

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Lucas, C., Kuehn, H., Zhao, F. et al. Dominant-activating germline mutations in the gene encoding the PI(3)K catalytic subunit p110δ result in T cell senescence and human immunodeficiency.Nat Immunol 15, 88–97 (2014). https://doi.org/10.1038/ni.2771

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• Received: 20 September 2013
• Accepted: 21 October 2013
• Published: 28 October 2013
• Issue Date: January 2014
• DOI: https://doi.org/10.1038/ni.2771