The structure of purified kinetochores reveals multiple microtubule-attachment sites (original) (raw)

• Pfau, S.J. & Amon, A. Chromosomal instability and aneuploidy in cancer: from yeast to man. EMBO Rep. 13, 515–527 (2012).
  Article CAS PubMed PubMed Central Google Scholar
• Compton, D.A. Mechanisms of aneuploidy. Curr. Opin. Cell Biol. 23, 109–113 (2011).
  Article CAS PubMed PubMed Central Google Scholar
• Santaguida, S. & Musacchio, A. The life and miracles of kinetochores. EMBO J. 28, 2511–2531 (2009).
  Article CAS PubMed PubMed Central Google Scholar
• Cheeseman, I.M. & Desai, A. Molecular architecture of the kinetochore-microtubule interface. Nat. Rev. Mol. Cell Biol. 9, 33–46 (2008).
  Article CAS PubMed Google Scholar
• Takeuchi, K. & Fukagawa, T. Molecular architecture of vertebrate kinetochores. Exp. Cell Res. 318, 1367–1374 (2012).
  Article CAS PubMed Google Scholar
• DeLuca, J.G. & Musacchio, A. Structural organization of the kinetochore-microtubule interface. Curr. Opin. Cell Biol. 24, 48–56 (2012).
  Article CAS PubMed Google Scholar
• Westermann, S., Drubin, D.G. & Barnes, G. Structures and functions of yeast kinetochore complexes. Annu. Rev. Biochem. 76, 563–591 (2007).
  Article CAS PubMed Google Scholar
• Zinkowski, R.P., Meyne, J. & Brinkley, B.R. The centromere-kinetochore complex: a repeat subunit model. J. Cell Biol. 113, 1091–1110 (1991).
  Article CAS PubMed Google Scholar
• Joglekar, A.P. et al. Molecular architecture of the kinetochore-microtubule attachment site is conserved between point and regional centromeres. J. Cell Biol. 181, 587–594 (2008).
  Article CAS PubMed PubMed Central Google Scholar
• Cheeseman, I.M., Chappie, J.S., Wilson-Kubalek, E.M. & Desai, A. The conserved KMN network constitutes the core microtubule-binding site of the kinetochore. Cell 127, 983–997 (2006).
  Article CAS PubMed Google Scholar
• Hofmann, C. et al. Saccharomyces cerevisiae Duo1p and Dam1p, novel proteins involved in mitotic spindle function. J. Cell Biol. 143, 1029–1040 (1998).
  Article CAS PubMed PubMed Central Google Scholar
• Li, Y. et al. The mitotic spindle is required for loading of the DASH complex onto the kinetochore. Genes Dev. 16, 183–197 (2002).
  Article CAS PubMed PubMed Central Google Scholar
• Welburn, J.P. et al. The human kinetochore Ska1 complex facilitates microtubule depolymerization-coupled motility. Dev. Cell 16, 374–385 (2009).
  Article CAS PubMed PubMed Central Google Scholar
• Hanisch, A., Sillje, H.H. & Nigg, E.A. Timely anaphase onset requires a novel spindle and kinetochore complex comprising Ska1 and Ska2. EMBO J. 25, 5504–5515 (2006).
  Article CAS PubMed PubMed Central Google Scholar
• Asbury, C.L., Tien, J.F. & Davis, T.N. Kinetochores′ gripping feat: conformational wave or biased diffusion? Trends Cell Biol. 21, 38–46 (2011).
  Article CAS PubMed Google Scholar
• Schittenhelm, R.B. et al. Spatial organization of a ubiquitous eukaryotic kinetochore protein network in Drosophila chromosomes. Chromosoma 116, 385–402 (2007).
  Article CAS PubMed PubMed Central Google Scholar
• Joglekar, A.P., Bloom, K. & Salmon, E.D. In vivo protein architecture of the eukaryotic kinetochore with nanometer scale accuracy. Curr. Biol. 19, 694–699 (2009).
  Article CAS PubMed PubMed Central Google Scholar
• Wan, X. et al. Protein architecture of the human kinetochore microtubule attachment site. Cell 137, 672–684 (2009).
  Article CAS PubMed PubMed Central Google Scholar
• Welburn, J.P. & Cheeseman, I.M. Toward a molecular structure of the eukaryotic kinetochore. Dev. Cell 15, 645–655 (2008).
  Article CAS PubMed Google Scholar
• McIntosh, J.R. et al. Fibrils connect microtubule tips with kinetochores: a mechanism to couple tubulin dynamics to chromosome motion. Cell 135, 322–333 (2008).
  Article CAS PubMed PubMed Central Google Scholar
• Dong, Y., Vanden Beldt, K.J., Meng, X., Khodjakov, A. & McEwen, B.F. The outer plate in vertebrate kinetochores is a flexible network with multiple microtubule interactions. Nat. Cell Biol. 9, 516–522 (2007).
  Article PubMed PubMed Central Google Scholar
• Johnston, K. et al. Vertebrate kinetochore protein architecture: protein copy number. J. Cell Biol. 189, 937–943 (2010).
  Article CAS PubMed PubMed Central Google Scholar
• Brinkley, B.R. & Stubblefield, E. The fine structure of the kinetochore of a mammalian cell in vitro. Chromosoma 19, 28–43 (1966).
  Article CAS PubMed Google Scholar
• Jokelainen, P.T. The ultrastructure and spatial organization of the metaphase kinetochore in mitotic rat cells. J. Ultrastruct. Res. 19, 19–44 (1967).
  Article CAS PubMed Google Scholar
• Roos, U.P. Light and electron microscopy of rat kangaroo cells in mitosis. II. Kinetochore structure and function. Chromosoma 41, 195–220 (1973).
  Article CAS PubMed Google Scholar
• Rieder, C.L. The structure of cold stable kinetochore microtubules in metaphase PtK1 cells. Chromosoma 84, 145–158 (1981).
  Article CAS PubMed Google Scholar
• Miranda, J.J., De Wulf, P., Sorger, P.K. & Harrison, S.C. The yeast DASH complex forms closed rings on microtubules. Nat. Struct. Mol. Biol. 12, 138–143 (2005).
  Article CAS PubMed Google Scholar
• Westermann, S. et al. Formation of a dynamic kinetochore- microtubule interface through assembly of the Dam1 ring complex. Mol. Cell 17, 277–290 (2005).
  Article CAS PubMed Google Scholar
• Akiyoshi, B. et al. Tension directly stabilizes reconstituted kinetochore-microtubule attachments. Nature 468, 576–579 (2010).
  Article CAS PubMed PubMed Central Google Scholar
• Akiyoshi, B., Nelson, C.R., Ranish, J.A. & Biggins, S. Quantitative proteomic analysis of purified yeast kinetochores identifies a PP1 regulatory subunit. Genes Dev. 23, 2887–2899 (2009).
  Article CAS PubMed PubMed Central Google Scholar
• Wigge, P.A. et al. Analysis of the Saccharomyces spindle pole by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. J. Cell Biol. 141, 967–977 (1998).
  Article CAS PubMed PubMed Central Google Scholar
• Enquist-Newman, M. et al. Dad1p, third component of the Duo1p/Dam1p complex involved in kinetochore function and mitotic spindle integrity. Mol. Biol. Cell 12, 2601–2613 (2001).
  Article CAS PubMed PubMed Central Google Scholar
• Tanaka, K. et al. Molecular mechanisms of kinetochore capture by spindle microtubules. Nature 434, 987–994 (2005).
  Article CAS PubMed Google Scholar
• Tien, J.F. et al. Cooperation of the Dam1 and Ndc80 kinetochore complexes enhances microtubule coupling and is regulated by aurora B. J. Cell Biol. 189, 713–723 (2010).
  Article CAS PubMed PubMed Central Google Scholar
• Lampert, F., Hornung, P. & Westermann, S. The Dam1 complex confers microtubule plus end-tracking activity to the Ndc80 kinetochore complex. J. Cell Biol. 189, 641–649 (2010).
  Article CAS PubMed PubMed Central Google Scholar
• Wei, R.R., Sorger, P.K. & Harrison, S.C. Molecular organization of the Ndc80 complex, an essential kinetochore component. Proc. Natl. Acad. Sci. USA 102, 5363–5367 (2005).
  Article CAS PubMed PubMed Central Google Scholar
• Wang, H.W. et al. Architecture and flexibility of the yeast Ndc80 kinetochore complex. J. Mol. Biol. 383, 894–903 (2008).
  Article CAS PubMed PubMed Central Google Scholar
• Alushin, G.M. et al. The Ndc80 kinetochore complex forms oligomeric arrays along microtubules. Nature 467, 805–810 (2010).
  Article CAS PubMed PubMed Central Google Scholar
• Hill, T.L. Theoretical problems related to the attachment of microtubules to kinetochores. Proc. Natl. Acad. Sci. USA 82, 4404–4408 (1985).
  Article CAS PubMed PubMed Central Google Scholar
• Westermann, S. et al. The Dam1 kinetochore ring complex moves processively on depolymerizing microtubule ends. Nature 440, 565–569 (2006).
  Article CAS PubMed Google Scholar
• Rose, M.D., Winston, F. & Heiter, P. Methods in Yeast Genetics, 198 (Cold Spring Harbor Laboratory Press, 1990).
• Longtine, M.S. et al. Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae. Yeast 14, 953–961 (1998).
  Article CAS PubMed Google Scholar
• Gelbart, M.E., Rechsteiner, T., Richmond, T.J. & Tsukiyama, T. Interactions of Isw2 chromatin remodeling complex with nucleosomal arrays: analyses using recombinant yeast histones and immobilized templates. Mol. Cell. Biol. 21, 2098–2106 (2001).
  Article CAS PubMed PubMed Central Google Scholar
• Sikorski, R.S. & Hieter, P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122, 19–27 (1989).
  CAS PubMed PubMed Central Google Scholar
• Ohi, M., Li, Y., Cheng, Y. & Walz, T. Negative Staining and Image Classification - Powerful Tools in Modern Electron Microscopy. Biol. Proced. Online 6, 23–34 (2004).
  Article CAS PubMed PubMed Central Google Scholar
• Stalling, D., Westerhoff, M. & Hege, H.-C. The Visualization Handbook (Elsevier, 2005).
• Kremer, J.R., Mastronarde, D.N. & McIntosh, J.R. Computer visualization of three-dimensional image data using IMOD. J. Struct. Biol. 116, 71–76 (1996).
  Article CAS PubMed Google Scholar
• Franck, A.D. et al. Tension applied through the Dam1 complex promotes microtubule elongation providing a direct mechanism for length control in mitosis. Nat. Cell Biol. 9, 832–837 (2007).
  Article CAS PubMed PubMed Central Google Scholar