Jamile Otman - Academia.edu (original) (raw)
Related Authors
University of the Basque Country, Euskal Herriko Unibertsitatea
Uploads
Papers by Jamile Otman
Brazilian Journal of Microbiology, 2007
Archives of Disease in Childhood - Fetal and Neonatal Edition, 1999
Journal of Clinical Microbiology, 2003
Enteropathogenic Escherichia coli (EPEC) has been associated with infantile diarrhea and mortalit... more Enteropathogenic Escherichia coli (EPEC) has been associated with infantile diarrhea and mortality in humans in developing countries. While diarrhea is also a major problem among primates kept in captivity, the role of E. coli is unclear. This study was designed to characterize diarrheagenic E. coli recovered from the feces of 56 New World nonhuman primates, primarily marmosets ( Callithrix spp.). Seventeen of the 56 primates had signs of diarrhea and/or enteritis. E. coli recovered from feces from these animals was tested by PCR for genes encoding virulence factors of diarrheagenic E. coli and for patterns of adherence to HeLa cells. In addition, isolates were characterized by the fluorescence actin staining test and by their ability to induce attaching and effacing lesions. PCR for the eae gene was positive in 10 of the 39 (27%) apparently healthy animals and in 8 of the 17 (47%) animals with diarrhea and/or enteritis. Colonies of eae + E. coli were serotyped and examined by PCR f...
Brazilian Journal of Microbiology, 2007
Archives of Disease in Childhood - Fetal and Neonatal Edition, 1999
Journal of Clinical Microbiology, 2003
Enteropathogenic Escherichia coli (EPEC) has been associated with infantile diarrhea and mortalit... more Enteropathogenic Escherichia coli (EPEC) has been associated with infantile diarrhea and mortality in humans in developing countries. While diarrhea is also a major problem among primates kept in captivity, the role of E. coli is unclear. This study was designed to characterize diarrheagenic E. coli recovered from the feces of 56 New World nonhuman primates, primarily marmosets ( Callithrix spp.). Seventeen of the 56 primates had signs of diarrhea and/or enteritis. E. coli recovered from feces from these animals was tested by PCR for genes encoding virulence factors of diarrheagenic E. coli and for patterns of adherence to HeLa cells. In addition, isolates were characterized by the fluorescence actin staining test and by their ability to induce attaching and effacing lesions. PCR for the eae gene was positive in 10 of the 39 (27%) apparently healthy animals and in 8 of the 17 (47%) animals with diarrhea and/or enteritis. Colonies of eae + E. coli were serotyped and examined by PCR f...